Objective：This study aims to investigate the effect of resveratrol（Res）on proliferation and collagen secretion in neonatal rat cardiac fibroblasts（CFs）and its possible mechanism. Methods：Neonatal rat CFs were isolated and identified by double enzyme digestion method，differential adhesion method and immunofluorescence from Sprague-Dawley rats born 1 day to 3 days. Two to three generations of CFs were randomly divided into four groups：Con（the normal control group），DMSO（the solvent control group），AngⅡ（the model group），AngⅡ+Res（the drug intervention group）. Then CCK8 and EdU staining kit were both used to detect cellular proliferation. Level of the cellular collagen secretion was measured by Hydroxyproline test kit. Moreover，the mRNA expression of AU-rich element RNA-binding factor 1（AUF1）and transforming growth factor-β1（TGF-β1）were examined by RT-qPCR，and the protein expression of AUF1 and TGF-β1 were detected by Western blot. Results：CFs were successfully extracted from SD rats and Vimentin was positive obviously. Compared with Con group，cellular proliferation and collagen secretion increased in AngⅡ group；the mRNA and protein expression of AUF1 and TGF-β1 increased as well. After the intervention of Res，AngⅡ+Res group had significantly lower cell proliferation and collagen secretion level than AngⅡ group；the mRNA and protein expression of AUF1 and TGF-β1 decreased as well. There was no statistically significant difference between the above indicators in the DMSO group and the Con group. Conclusion：Res can inhibit AngⅡ-induced proliferation and collagen secretion of cardiac fibroblasts，which may be related to the suppression of AUF1 and TGF-β1 expression.