Objective：To investigate effects and mechanisms of PKM2 on hepatocellular carcinoma（HCC）cell. Methods：PKM2 expression was detected in liver cancer tissues and liver cancer cell lines（HepG2，Hep3B，HuH7 and smmc-7721）by quantitative PCR（qPCR），Western blot（WB）and immunohistochemistry（IHC）. PKM2 expression was inhibited by small interfering RNA（siRNA）in HCC cell lines. Effects of siRNA on the proliferation，invasion and migration was analyzed in HCC cell lines. In addition，we studied the effect of PKM2 siRNA on Hippo signaling pathway. Results：qPCR，WB and IHC showed PKM2 expression was significantly higher in HCC tissues than paracancer tissue，and in HCC cell lines than normal liver cell line（L02）. PKM2 siRNA effectively inhibited the proliferation，invasion and migration of HCC cells. Further study confirmed that Hippo signaling activity was inhibited in HCC cell lines compared with L02，and was enhanced in PKM2 siRNA-treated HCC cell lines. Inhibition of Hippo signaling activity significantly reversed inhibition of proliferation，invasion and migration in PKM2 siRNA-mediated HCC cell lines. Conclusion：PKM2 may inhibit phosphorylation of LAST1 and YAP，decrease Hippo signaling pathway，and promote HCC cell proliferation，invasion and migration.