白藜芦醇诱导胃癌BGC⁃823细胞凋亡的分子机制
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吉林省省级产业创新专项资金项目(2018C049?4);吉林省教育厅科研项目[吉教科合字(2016)第133号];吉林市科技局项目(201750259);吉林化工学院校级重大项目(20180101)


Molecular mechanism of apoptosis in gastric cancer BGC823 cells induced by resveratrol
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    摘要:

    目的:探究白藜芦醇对人胃癌细胞BGC823的作用及其分子机制。方法:采用MTT法检测白藜芦醇对BGC823细胞增殖的影响;流式细胞术测定白藜芦醇对BGC823细胞凋亡、活性氧水平和线粒体膜电位的影响;Western blot法检测白藜芦醇对BGC823细胞cleaved caspase-3和cleaved caspase-9蛋白表达的影响。结果:MTT结果显示白藜芦醇对胃癌BGC823细胞具有明显的增殖抑制作用,且呈时间和剂量依赖关系;31.25~500.00 μmol/L白藜芦醇作用24 h和48 h可诱导BGC823细胞凋亡,500 μmol/L作用24 h和48 h,细胞凋亡率分别达(40.42±2.64)%和(75.02±2.36)%。流式细胞术结果显示白藜芦醇作用24 h后可使BGC823细胞内活性氧(reactive oxygen species,ROS)增多;与对照组相比,62.50~500.00 μmol/L白藜芦醇作用细胞24 h后,细胞出现了显著的线粒体膜电位降低(P < 0.05);Western blot结果显示白藜芦醇以浓度依赖方式上调cleaved caspase-9和cleaved caspase-3的表达。结论:白藜芦醇可以显著诱导细胞凋亡,其诱导细胞凋亡与ROS增多、线粒体膜电位降低和调节cleaved caspase-9和cleaved caspase-3的表达有关。

    Abstract:

    Objective:This study aims to investigate the anticancer activities and molecular mechanisms of resveratrol on human gastric cancer BGC823 cells. Methods:Growth of BGC823 cells affected by resveratrol was detected by MTT assay. Effects of resveratrol on apoptosis,reactive oxygen species levels and mitochondrial membrane potential of BGC823 were determined by flow cytometry. Expression changes of cleaved caspase-3 and cleaved caspase-9 proteins in BGC823 cells affected by resveratrol were detected by Western blot. Results:The results of MTT showed resveratrol inhibited the proliferation of BGC823 in a time-and dose-dependent manner. Treatment with 31.25-500.00 μmol/L resveratrol for 24 h and 48 h induced apoptosis of BGC823 cells,and the apoptosis rate reached (40.42±2.64)% and (75.02±2.36)% in resveratrol concentration of 500 μmol/L at 24 h and 48 h,respectively. Flow cytometric analysis showed that BGC823 cells treated with resveratrol were accumulated in ROS levels in cells for 24 h. Compared with the control group,BGC823 cells showed a significantly decreased in mitochondrial membrane potential after treated with 62.50-500.00 μmol/L resveratrol for 24 h(P < 0.05). Western blot showed that expressions of cleaved caspase-9 and cleaved caspase-3 protein in BGC823 cells were increased after resveratrol treatment in a dose-dependent manner. Conclusion:Resveratrol can significantly induce cell apoptosis,which is related to the increase of ROS,the decrease of mitochondrial membrane potential and the regulation of cleaved caspase-9 and cleaved caspase-3 expression.

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于 明,荣家闵,葛雅琨,张仁文,劳 军,张元新.白藜芦醇诱导胃癌BGC⁃823细胞凋亡的分子机制[J].南京医科大学学报(自然科学版),2019,(9):1292-1297

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  • 收稿日期:2019-01-05
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  • 在线发布日期: 2019-09-29
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