二甲双胍对脂多糖诱导的大鼠心肌细胞H9C2损伤的保护机制
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

上海市浦东新区科经委项目(PKJ2018?Y53)


Protective mechanism of metformin on injury of rat cardiomyocyte H9C2 induced by lipopolysaccharide
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:研究二甲双胍(metformin,Met)对脂多糖(lipopolysaccharide,LPS)诱导的大鼠心肌细胞H9C2损伤的保护作用及机制。方法:心肌细胞H9C2按照不同处理方式分为对照组、LPS组及3种不同浓度Met与LPS联合培养组。培养24 h后采用MTT法测定各组细胞活力,流式细胞技术检测各组细胞凋亡和活性氧水平,蛋白质印迹法检测各组Caspase3、BCL2、BAX、腺苷酸活化蛋白激酶(adenosine 5′-monophosphate -activated protein kinase,t-AMPK)、磷酸化腺苷酸活化蛋白激酶(phosphorylated adenosine 5′-monophosphate -activated protein kinase,p-AMPK)、Beclin1、Parkin的蛋白表达。结果:和LPS单独处理组相比,Met与LPS联合培养降低了LPS诱导的细胞活力增强和细胞凋亡增多,降低了BCL2、BAX蛋白表达,Caspase3表达未降低;p-AMPK、Beclin1、Parkin蛋白表达增加,细胞活性氧水平降低。结论:Met可能通过活化AMPK途径,增加细胞自噬、减少活性氧产生,减轻脂多糖对细胞的损伤,保护心肌细胞。

    Abstract:

    Objective:This study aims to investigate the protective mechanism of metformin(Met)on rat cardiomyocytes H9C2 injury induced by lipopolysaccharide(LPS). Methods:Rat cardiomyocytes H9C2 were divided into Control group,LPS group,Met of different concentrations added with LPS group,according to different treatments. Cells were cultured for 24 hours. MTT was used to detect the cell viability,and intracellular reaction oxygen species and apoptosis were detected by the flow cytometry. The protein levels of Caspase3,BCL2,BAX,t-AMPK(adenosine 5′-monophosphate -activated protein kinase),p-AMPK(phosphorylated adenosine 5′-monophosphate -activated protein kinase),Beclin1 and Parkin were measured by Western blot. Results:Compared with LPS treatment alone,combine of Met and LPS decreased cell viability and apoptosis induced by LPS. The expressions of BCL2 and BAX proteins were decreased,while the Caspase3 protein expression was not decreased. And the expressions of p-AMPK,Beclin1 and Parkin proteins were increased,and the level of reactive oxygen species was deduced. Conclusion:Met may increase autophagy and reduce the production of reactive oxygen species by activating AMPK pathway,alleviate the damage of LPS to cells and protect myocardial cells.

    参考文献
    相似文献
    引证文献
引用本文

高 婷,陈 忠.二甲双胍对脂多糖诱导的大鼠心肌细胞H9C2损伤的保护机制[J].南京医科大学学报(自然科学版),2019,(9):1298-1303

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2019-02-01
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2019-09-29
  • 出版日期:
通知关闭
郑重声明