Effects of endogenous peptide P1DA on proliferation，migration and invasion of ovarian cancer cells
Author:
Affiliation:
Fund Project:
摘要
|
图/表
|
访问统计
|
参考文献
|
相似文献
|
引证文献
|
资源附件
|
文章评论
摘要:
目的：探讨卵巢组织中来源于AHNK2蛋白的多肽（peptide 1 derived from AHNK2 protein，P1DA）对卵巢癌细胞系SKOV3、OVCAR3增殖、迁移、侵袭和凋亡的影响。方法：P1DA处理对数生长期的SKOV3和OVCAR3细胞后，CCK-8法检测细胞活性变化，以探索P1DA对卵巢癌SKOV3和OVCAR3细胞增殖的影响；Transwell实验检测P1DA处理后细胞迁移、侵袭能力变化；流式细胞术检测P1DA处理后细胞凋亡率变化，分析P1DA对两种卵巢癌细胞凋亡率的影响。结果：P1DA处理后，卵巢癌细胞SKOV3及OVCAR3的细胞活性明显增加，迁移、侵袭能力明显增加，细胞凋亡率则无明显变化。结论：卵巢组织内源性肽P1DA可促进卵巢癌SKOV3及OVCAR3细胞的增殖、迁移和侵袭能力，对其凋亡无显著影响。
Abstract:
Objective：This study aims to investigate the effects of ovarian tissue endogenous peptide 1 derived from AHNK2 protein（P1DA）on the proliferation，migration，invasion and apoptosis of ovarian cancer cell lines of SKOV3 and OVCAR3. Methods：SKOV3 and OVCAR3 cells in logarithmic growth phase were treated with P1DA. The cell activity was detected by CCK-8 assay to explore the effect of P1DA on the proliferation of SKOV3 and OVCAR3 cells. Transwell experiment was performed to detect the migration and invasion of SKOV3 and OVCAR3 cells treated with P1DA. Flow cytometry analysis was performed to analyze the apoptosis of SKOV3 and OVCAR3 cells treated with P1DA. And the effects of P1DA on apoptosis of ovarian cancer cells were analyzed. Results：The cell activity of SKOV3 and OVCAR3 cells treated with P1DA was significantly increased，the migrated and invaded cells in chamber were increased，and no difference was found in apoptosis of cells after treated with P1DA. Conclusion：Ovarian tissue endogenous peptide P1DA can promote the proliferation，migration and invasion of SKOV3 and OVCAR3，but has no effect on cell apoptosis.
