Objective：Chronic periodontitis patients often suffer from dentin hypersensitivity（DH） due to gingival recession or root surface exposure . The aim of the study is to evaluate the changes of dentin tablets and hypersensitivity teeth after Nd：YAG laser，Gluma desensitizer and their combination treatment. Methods：Forty-eight severe periodontitis teeth were extracted and randomly divided into four groups：Control group，Nd：YAG laser group，Gluma desensitizer group and Gluma +Nd：YAG group. The Scanning electron microscopy（SEM）was used to observe the opening of dentinal tubules. One hundred DH teeth from 30 patients with periodontitis were selected and randomly divided into four groups as above；Visual analogue scale（VAS）was used to assess the sensitivity of clinical patients after temperature and mechanical stimuli at baseline and one month after treatment. Results：SEM showed that the diameter of dentinal tubules of Nd：YAG laser group，Gluma desensitizer group and Gluma +Nd：YAG group were smaller than that of the control group（P < 0.05），and the exposure rate was significantly decreased in Gluma +Nd：YAG group（P < 0.05）. The VAS of Nd：YAG laser group，Gluma desensitizer group and Gluma +Nd：YAG group was significantly lower after treatment than before treatment（P < 0.05）. The Gluma +Nd：YAG group obtained the most effective results than any other groups，which differed significantly from the control group（P < 0.05）. Conclusion：Combining Gluma and Nd：YAG to treat DH can effectively reduce dentinal tubules exposed and the sensitivity of DH teeth.