Objective：To investigate the effect of geniposide on activity and endocytosis of murine macrophage RAW264.7 cells. Methods：The RAW264.7 cells were added with geniposide at different concentrations and further cultured for several hours. The proliferation of RAW264.7 cells was measured by CCK-8 assay. According to the results of CCK-8 assay，geniposide at appropriate concentrations were selected to culture with RAW264.7 cells for 24 h，and subsequently detected the pinocytosis activity of neutral red solution. After cultured with geniposide at the optimal concentration（2 mg/mL），RAW264.7 cells were detected the phagocytosis activity of Pseudomonas aeruginosa and intracellular bacterial survival. Results：Geniposide showed little cytotoxicity on RAW264.7 cells. The proliferation of RAW264.7 cells was not seriously affected by 0~8 mg/mL geniposide. Geniposide at 2 mg/mL could significantly promote the pinocytosis of neutral red solution by RAW264.7 cells，but inhibited the phagocytosis of Pseudomonas aeruginosa and intracellular bacterial survival. Conclusion：Geniposide has dual regulatory effect on the pinocytosis and phagocytosis of RAW264.7 cells.