静磁场连续曝磁对牙髓干细胞增殖和分化的影响
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金(81771044);中国博士后基金(2015M571647);江苏省博士后基金(1402044B);江苏高校优势学科建设工程资助项目(2018-87);江苏省青年医学人才(QNRC2016853);东南大学南京医科大学合作研究项目(2242018K3DN16)


Effects of continuous exposure to static magnetic field on the proliferation and differentiation of dental pulp stem cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:研究静磁场连续曝磁对牙髓干细胞增殖和分化的影响。方法:搭建静磁场下细胞培养装置,磁通强度(35±5)mT,以常规培养的细胞为对照组。用扫描电镜检测磁场下与常规培养细胞的形态,以CCK-8检测细胞的增殖情况,同时检测细胞的碱性磷酸酶活性,并进行茜素红染色以说明矿化物形成情况。结果:扫描电镜显示,磁性培养的细胞与常规培养的细胞在形态上没有显著差异;磁场下与常规培养下的细胞增殖情况也没有差异;但是磁场下细胞的碱性磷酸酶活性显著增高(P < 0.05),且矿化物的形成也显著增多(P < 0.05)。结论:静磁场连续曝磁对牙髓干细胞的形态和增殖没有影响,但促进了其成骨方向的分化。

    Abstract:

    Objective:This stady aims to observe the effects of continuous exposure to static magnetic field(SMF)on the proliferation and differentiation of dental pulp stem cells(DPSCs). Methods:The magnetic cell culture set was used with the flux intensity about (35 ± 5)mT. The normal cultured cells were used as control. The morphology of the cells was detected by scanning electron microscopy(SEM). The cell proliferation was detected by CCK-8,and the alkaline phosphatase activity(ALP)was also measured. Alizarin red staining(ARS) was used to detect the mineral synthesis. Results:There were no significant differences in SEM images and cell proliferation between magnetic cultured cells and normal cultured cells,but the ALP activity of magnetic cultured cells was significantly increased(P < 0.05),and the mineralization formation of cells under magnetic culture was significantly more than that of normal cultured cells(P < 0.05). Conclusion:Continuous exposure to SMF neither changed the cell morphology nor the cell proliferation,but significantly promoted the differentiation of DPSCs.

    参考文献
    相似文献
    引证文献
引用本文

夏 阳,陈慧敏,胡姝颖,孙剑飞,郭 宇,贾 璐.静磁场连续曝磁对牙髓干细胞增殖和分化的影响[J].南京医科大学学报(自然科学版),2020,(2):191-194

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2019-03-17
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2020-03-14
  • 出版日期:
通知关闭
郑重声明