双酚S暴露对小鼠脑⁃肠⁃菌轴神经递质代谢稳态的影响
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金(81773484)


Bisphenol S exposure affected the mice neurotransmitter metabolism homeostasis through the brain⁃gut⁃microbiota axis
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨双酚S(bisphenol S,BPS)暴露对小鼠脑-肠-菌轴神经递质代谢稳态的影响。方法:通过皮下注射建立C57BL/6J雄性小鼠BPS亚慢性暴露模型,基于UHPLC-MS/MS液质联用技术检测前额皮层、血清和肠中色氨酸与酪氨酸通路神经递质的水平;通过16S rRNA基因测序分析技术检测小鼠肠道菌群的表达。结果:BPS暴露引起小鼠前额皮层、血清和肠道中色氨酸与酪氨酸通路多种神经递质代谢紊乱:色氨酸通路中,与对照组小鼠相比,BPS组小鼠前额皮层、血清及肠道中的5-羟色胺(5-hydroxytryptamine,5-HT)含量降低,3-羟基犬尿氨酸(3-HK)在皮层与血清中显著上升;酪氨酸通路中,BPS暴露组小鼠前额皮层中左旋多巴(L-dopa)、多巴胺(dopamine,DA)水平明显上升,而在肠道中显著降低。此外,BPS暴露小鼠肠道菌群出现区别于对照小鼠的差异性分布,且不动杆菌属、乳杆菌属和一种未分类的梭菌属在两组间比较具有显著性差异。结论:BPS暴露可通过脑-肠轴影响中枢及外周色氨酸通路与酪氨酸通路神经递质代谢,同时可造成肠道菌群紊乱。

    Abstract:

    Objective:To investigate the effect of bisphenol S(BPS)exposure on the homeostasis of neurotransmitter metabolism in the mice brain-gut-microbiota axis. Methods:A subchronic BPS exposure model using male C57BL/6J mice was established. The neurotransmitters in tryptophan and tyrosine pathways in the prefrontal cortex,serum and intestine were detected with UHPLC-MS/MS. The gut microbiota analysis was detected based on 16S rRNA gene sequencing. Results:BPS exposure resulted in neurotransmitter metabolism disorders of tryptophan and tyrosine pathways in the mice prefrontal cortex,serum and intestine. In the tryptophan pathway,when compared with the control group,5-HT was decreased in the prefrontal cortex(PFC),serum and intestine of the BPS-exposed group,while 3-HK was greatly increased in the PFC and serum. In the tyrosine pathway,BPS exposure significantly increased L-dopa and DA levels in the PFC,but decreased the counterparts in the intestine. Additionally,BPS exposure led to a differential distribution of gut microbiota in BPS-exposure mice. In the genus level,the Acinetobacter,Lactobacillus and an unclassified_Clostridiales exerted great differences between the two groups. Conclusion:BPS exposure can affect central and peripheral neurotransmitter metabolism in tryptophan pathway and tyrosine pathway through the brain-gut axis. Additionally,BPS exposure caused gut microbiota disturbance.

    参考文献
    相似文献
    引证文献
引用本文

邓媛媛,王 军,姚佳希,余 静,陈旭锋,高 蓉.双酚S暴露对小鼠脑⁃肠⁃菌轴神经递质代谢稳态的影响[J].南京医科大学学报(自然科学版),2020,(5):663-668

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2019-10-29
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2020-06-10
  • 出版日期:
通知关闭
郑重声明