Objective：This study aims to prepare bionic bone scaffolds using 10 times simulated body fluid（10×SBF）mineralized silk fibroin/chitosan（SF/CS）electrospun scaffolds，and to compare the physical properties and the ability of inducing osteogenic differentiation of cells before and after mineralization. Methods：The compositions and properties of the scaffolds before and after mineralization were compared by scanning electron microscopy（SEM），Fourier transform infrared spectroscopy（FT-IR），water absorption and mechanical properties. The effects of biomimetic mineralization on the adhesion and proliferation of human bone marrow mesenchymal stem cells（HBMSC）on scaffolds were compared by living cell staining and Cell Counting Kit-8（CCK-8）. Alkaline phosphatase（ALP）staining and alizarin red staining（ARS）were used to compare the osteoinductive ability of the scaffolds before and after mineralization. Results：SEM，FT-IR，water absorption and mechanical properties confirmed the success of mineralization and the improvement of physical properties. Live cell staining and CCK-8 showed that biomimetic mineralization did not increase the cytotoxicity of the scaffolds. ALP and ARS showed that the scaffolds after mineralization were more likely to induce osteogenic differentiation of HBMSC. Conclusion：SBF biomimetic mineralized scaffolds are more suitable for bone tissue engineering.