The effects of inhibitor of differentiation 3 on female mice fertility
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摘要:
目的：通过构建Id3-Gdf9条件性敲除（CKO）小鼠并研究雌性小鼠的表型，从而确定分化抑制因子3（inhibitor of differentiotion 3，Id3）对雌性小鼠生育能力的影响。方法：通过交配繁殖获得Id3-Gdf9 CKO小鼠，以HE染色、卵泡计数对CKO小鼠卵巢中的卵泡发育情况进行检测，以卵母细胞体外成熟实验和体外超排实验对卵母细胞的体外成熟进行检测，通过交配实验对生育力进行检测。结果：qPCR、Western blot结果显示卵母细胞中Id3被成功敲除；对3、8周小鼠卵巢进行HE染色并对各级卵泡进行计数，结果表明CKO小鼠中各级卵泡发育正常；卵母细胞体外成熟以及体外超排实验显示CKO小鼠中卵母细胞体外成熟正常；交配实验表明CKO小鼠生育能力正常。结论：在正常饲养条件下，条件性敲除Id3不影响正常卵泡的发育以及雌性小鼠的生育能力。
Abstract:
Objective：The phenotype of female mice was studied by constructing Id3-Gdf9-Cre+ conditional knockout（CKO）mice to further determine the effect of differentiation inhibitor Id3 on female mice fertility. Methods：The development of follicles was detected in the ovaries of the conditional knockout mice with Id3-Gdf9-Cre+ by HE staining and follicle counting. The oocyte maturation was detected by maturation experiments in vitro and superovulation experiments in vitro. And mice fertility was detected by mating experiment. Results：The results of qPCR and Western blot showed that we had successfully knocked out Id3 in oocytes. HE staining was performed on 3 weeks and 8 weeks mice ovaries and the follicles were counted at various levels，and the follicles developed at all levels were normal. Oocyte maturation experiments and superovulation experiments showed that oocyte maturation was normal in vitro. Mating experiment showed that CKO mice have normal fertility. Conclusion：Under normal feeding conditions，conditional knockout of Id3 does not affect normal follicles development or fertility of female mice.
