3D打印PLGA支架修复大鼠上颚骨缺损的实验研究
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国家自然科学基金(81771044);东南大学?南京医科大学合作研究项目(2242018K3DN16);江苏省青蓝工程项目,江苏省青年医学人才(QNRC2016853);江苏高校优势学科建设工程资助项目(2018?87)


Effects of PLGA scaffold fabricated by 3D printing on the repair of rat palate bone defect
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    摘要:

    目的:探讨3D打印聚乳酸-羟基乙酸共聚物(poly lactic-co-glycolic acid,PLGA)支架修复大鼠上颚骨缺损的效果。方法:将PLGA 溶解于丙酮中,利用3D打印机打印出间距为100 μm、单层厚度为60 μm的圆盘形网格状支架材料。将12只大鼠随机分为2组(n=6),制备直径为3 mm的上颚圆形骨缺损,实验组在骨缺损中植入3D打印的PLGA支架后缝合关闭缺损,对照组术后仅缝合关闭缺损。术后8周处死大鼠,行micro-CT及组织学检测观察缺损处骨组织修复情况。结果:成功制备3D打印PLGA支架。大鼠上颚骨缺损造模后8周,植入3D打印PLGA支架的实验组骨体积分数(bone-volume/total-volume,BV/TV)大于对照组(P < 0.05),骨缺损未愈合面积小于对照组(P < 0.05)。通过上颚骨组织HE染色,发现实验组骨缺损边缘有大量新生骨质,而对照组只有少量新生骨质。结论:3D打印PLGA支架能够促进大鼠上颚骨缺损的骨再生。

    Abstract:

    Objective:To observe the effects of 3D-printed poly lactic-co-glycolic acid(PLGA)scaffold on the repair of rat palate bone defect. Methods:The PLGA was dissolved in acetone,and disc-shaped mesh scaffolds having pores with diameters of 100 μm and a single layer thickness of 60 μm were printed using a 3D printer. Twelve rats were randomly divided into two groups(n=6)to create round palate bone defects with a diameter of 3 mm. The bone defect in the experimental group was implanted with a 3D-printed PLGA scaffold and sutured to close the defect,while in the control group the defect was sutured to close only. The rats were sacrificed after 8 weeks,and the bone defect repair was evaluated by using micro-CT and histological analysis. Results:3D-printed PLGA scaffolds were successfully fabricated. Eight weeks after the establishment of the palate defects model in rats,the bone-volume/total-volume(BV/TV)of the experimental group implanted with the 3D-printed PLGA scaffold was significantly higher than the control group(P < 0.05),and the unhealed area of the bone defect was also smaller than that of the control group(P < 0.05). HE staining of the palate bone tissue showed that a large amount of new bone was formed at the edge of the defect in the experimental group,while the control group had only a small amount of new bone. Conclusion:3D-printed PLGA scaffold can promote the bone regeneration in palate bone defect.

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杨祖坤,贾 璐,韩利萍,孙柳絮,郭 宇,夏 阳.3D打印PLGA支架修复大鼠上颚骨缺损的实验研究[J].南京医科大学学报(自然科学版),2020,(8):1125-1129

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  • 收稿日期:2019-11-10
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  • 在线发布日期: 2020-08-21
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