Gef26对果蝇原代细胞F⁃actin的调控作用
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国家自然科学基金(30928014,31600824)


Gef26 regulates F⁃actin organization in Drosophila primary culture
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    摘要:

    目的:探讨Gef26对果蝇原代细胞发育的影响。方法:培养野生型、gef26突变体(gef266/+和gef263/+)以及gef266/+;Abl4/+双突变体果蝇胚胎的原代细胞,观察细胞形态。对细胞骨架F-actin进行荧光染色,观察细胞骨架的分布。结果:在果蝇胚胎的原代细胞中,野生型肌肉细胞形态正常,gef26突变体的肌肉细胞形态明显异常。gef266/+;Abl4/+双突变体肌肉细胞形态正常,即阿贝尔森酪氨酸激酶(Abelson tyrosine kinase,Abl)突变挽救gef26突变体肌肉细胞形态异常的表型。野生型肌肉细胞中的F-actin呈现规整的形态和连续排布,而两株gef26突变体肌肉细胞中的F-actin排布却呈现出不规整形态和不连续排布。gef266/+;Abl4/+双突变体细胞中的F-actin呈现出规整的形态和连续的排布,即Abl突变挽救gef26突变体F-actin组装紊乱的表型。结论:Gef26可能通过Abl信号通路调控肌动蛋白细胞骨架的组装。

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    Objective:To investigate the role of Gef26 in Drosophilia primary culture. Methods:Through primary culture of Drosophilia wide type,gef26 mutants and gef266/+;Abl4/+ mutants,the cell shape is observed,and by fluorescent staining of F-actin the distribution of cytoskeleton can be observed. Results:In Drosophila primary culture, wild type muscle cells have normal morphology, and the morphology of muscle cells of the gef26 mutants is obviously abnormal. The gef266/+;Abl4/+ mutants muscle cells have a normal morphology, namely the phenotype of gef26 mutant muscle cells with abnormal morphology rescued by Abelson tyrosine kinase(Abl) mutation. F-actin in wild type muscle cells showed a regular morphology and continuous arrangement, while that in the gef26 mutants muscle cells showed irregular morphology and discontinuous arrangement. Meanwhile, F-actin in gef266/+; Abl4/+ double mutant cells showed a regular morphology and continuous arrangement, that is, the phenotype of gef26 mutant with disordered F-actin arrangement rescued by Abl mutation. Conclusion:Gef26 regulates cell cytoskeleton organization through Abl signaling during muscle cells development.

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张晨晨,刘力娟,欧梦竹. Gef26对果蝇原代细胞F⁃actin的调控作用[J].南京医科大学学报(自然科学版),2020,(10):1431-1436

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  • 收稿日期:2019-08-14
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  • 在线发布日期: 2020-11-10
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