苦豆碱对缺氧/复氧诱导人脐静脉内皮细胞凋亡的影响
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Effects of aloperin on the apoptosis of human umbilical vein endothelial cells induced by hypoxia/reoxygenation
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    摘要:

    目的:探讨苦豆碱对缺氧/复氧诱导的人脐静脉内皮细胞凋亡的影响。方法:培养人脐静脉内皮细胞,随机分为对照组、缺氧/复氧组、苦豆碱(20、50和100 mg/L)预处理组和苦豆碱预处理+LY294002组,并进行相应处理。MTT法检测细胞增殖,流式细胞术检测细胞凋亡,比色法检测Caspase-3活性,Western blot法检测Bax、Bcl-2、Akt和p-Akt蛋白表达。结果:苦豆碱可提高缺氧/复氧抑制的人脐静脉内皮细胞增殖(P < 0.05),显著减少缺氧/复氧诱导的细胞凋亡(P < 0.05),逆转缺氧/复氧引起的Caspase-3活性和Bax表达的增加、Bcl-2表达的降低,提高Bcl-2/Bax值(P < 0.05)。此外,苦豆碱预处理可明显上调缺氧/复氧降低的p-Akt蛋白表达(P < 0.05),而PI3K/Akt抑制剂LY294002明显逆转苦豆碱抗缺氧/复氧诱导的凋亡作用(P < 0.05)。结论:苦豆碱通过激活PI3K/Akt通路抑制缺氧/复氧诱导的人脐静脉内皮细胞凋亡。

    Abstract:

    Objective:To investigate the protective effect of aloperin on the apoptosis of human umbilical vein endothelial cells(HUVECs)induced by hypoxia/reoxygenation. Methods:HUVECs were cultured and randomly divided into the control group,the hypoxia/reoxygenation group,the aloperin(20,50 and 100 mg/L)pretreatment group and the aloperin+LY294002 group,and treated accordingly. Cell proliferation and apoptosis were detected by MTT assay and flow cytometry,respectively. Caspase-3 activity was detected by colorimetry,and protein expression of Bax,Bcl-2,Akt and p-Akt were detected by Western blot. Results:Aloperin reversed the decrease of proliferation and increase of apoptosis induced by hypoxia/reoxygenation in human umbilical vein endothelial cells(P < 0.05). Aloperin alleviated the increase of Caspase-3 activity and Bax expression,and the downregulation of Bcl-2 induced by hypoxia/reoxygenation,and increased Bcl-2/Bax value(P < 0.05). In addition,aloperin significantly upregulated p-Akt protein expression(P < 0.05),while the anti-apoptosis effect of aloperin against hypoxia/reoxygenation was significantly reversed by PI3K/Akt inhibitor LY294002(P < 0.05). Conclusion:Aloperin could inhibit hypoxia/reoxygenation-induced apoptosis of HUVEC cells by activating the PI3K/Akt pathway.

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马会军.苦豆碱对缺氧/复氧诱导人脐静脉内皮细胞凋亡的影响[J].南京医科大学学报(自然科学版),2020,(10):1472-1477

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  • 收稿日期:2019-06-23
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  • 在线发布日期: 2020-11-10
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