Objective：This study aims to investigate the expression patterns of lncRNA HAND2-AS1，miRNA hsa-miR-520c-3p and integrin subunit beta 8（ITGB8） in placentas of preeclampsia（PE） patients and the role of HAND2-AS1 played in modulating vascular endothelial cells. Methods：Total 25 placentas of PE patients and 20 placentas of healthy controls were collected to detect the expressing abundance of HAND2-AS1，hsa-miR-520c-3p and ITGB8 using qRT-PCR technology. Proliferation，migration and angiogenesis of human umbilical vein endothelial cell（HUVEC） was detected through cell viability assay，wound healing assay and tube formation assay，respectively，when HAND2-AS1 was overexpressed or knock-down. Also，expression of hsa-miR-520c-3p and ITGB8 was detected using QPCR. Results：Compared with healthy controls，expression of HAND2-AS1 and ITGB8 increased and expression of hsa-miR-520c-3p decreased significantly in placentas of PE patients. In placentas of PE patients，the expression of HAND2-AS1 was negatively correlated with hsa-miR-520c-3p and positively correlated with ITGB8. The proliferation，migration and angiogenesis of HUVEC were impaired，the expression of hsa-miR-520c-3p decreased and the expression of ITGB8 increased when HAND2-AS1 was overexpressed. The result was the opposite when HAND2-AS1 was knocked-down. Conclusion：HAND2-AS1 inhibits proliferation，migration and angiogenesis of vascular endothelial cells，of which potential mechanism may be modulating expression of hsa-miR-520c-3p/ITGB8.