YY1通过下调长链非编码RNA SOX2OT抑制胰腺癌细胞的迁移和侵袭
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国家自然科学基金(81572337)


YY1 suppresses migration and invasion of pancreatic ductal adenocarcinoma cells by downregulating lncRNA SOX2OT
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    摘要:

    目的:研究转录因子YY1对胰腺癌细胞迁移和侵袭的影响和分子机制。方法:使用定量RT-PCR和Western blot检测YY1和SOX2OT的表达。通过划痕实验和Transwell侵袭实验,研究YY1在胰腺癌细胞迁移和侵袭中的作用。在YY1过表达或YY1敲低的胰腺癌细胞中,通过SOX2OT过表达或RNA干扰进行功能回复实验。结果:发现YY1过表达抑制BXPC-3和PANC-1细胞中的SOX2OT表达,相反,YY1干扰促进SOX2OT的表达。此外,YY1过表达抑制BXPC-3细胞在体外的迁移和侵袭,相反YY1干扰促进细胞的迁移和侵袭。在YY1过表达的BXPC-3细胞中过表达SOX2OT,或者在YY1敲低的细胞中干扰SOX2OT的表达,可以逆转YY对细胞迁移和侵袭的作用。结论:YY1通过下调SOX2OT表达抑制胰腺癌细胞的迁移和侵袭。

    Abstract:

    Objective:This study aims to observe the effects and mechanisms of transcription factor YY1 on the migration and invasion of pancreatic cancer cells. Methods:In this study,YY1 and SOX2OT expression were detected by using quantitative RT-PCR and Western blot. The role of YY1 in the migration and invasion of pancreatic cancer cells was studied by cell wound healing assays and transwell cell invasion assays. The functional recovery experiments were performed by overexpression or RNA interference of SOX2OT in YY1 overexpression or YY1 knockdown pancreatic cancer cells. Results:It was found that YY1 overexpression decreased,while YY1 knockdown increased SOX2OT expression in BXPC-3 and PANC-1 cells. Furthermore,YY1 overexpression suppressed,whereas YY1 knockdown enhanced,the migration and invasion properties of BXPC-3 cells in vitro. Overexpression of SOX2OT in YY1 overexpressed BXPC-3 cells or RNA interference of SOX2OT in YY1 knockdown cells can partly reverse the effect of YY on cell migration and invasion. Conclusion:The present study suggested that YY1 suppresses migration and invasion of pancreatic cancer cells in a SOX2OT-dependent mechanism.

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汪冬艳,张静静. YY1通过下调长链非编码RNA SOX2OT抑制胰腺癌细胞的迁移和侵袭[J].南京医科大学学报(自然科学版),2021,(2):187-192

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  • 收稿日期:2020-04-30
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  • 在线发布日期: 2021-03-10
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