Objective：This study aims to establish a genotyping method based on the iPLEX analysis of MassARRAY platform to detect human platelet antigen （HPA）. Methods：The information of single nucleotide polymorphism mutation or base deletion in HPA1-29W system was searched by literature，and 29 gene mutations were identified. According to MassARRAY mutation site labeling method，primer design fixed format and primer design software，a total of 90 primers（30 forward，30 reverse amplification primers and 30 extension primers）were designed online. The sensitivity，specificity and repeatability were analyzed using 12 synthetic plasmids which were inserted into HPA1-29W wild-type or mutant sequences and 50 samples with known HPA typing. The results of 15 randomly selected samples were screened by the first generation sequencing and mass spectrometry typing to establish the detection method. Results：The results of mass spectrometry based on MassARRY platform were consistent with that of the sequencing method. The sensitivity，specificity and repeatability rate of the mass spectrum method were 100%，100% and 100%，respectively. Conclusion：The method of HPA1-29W gene mass spectrometry based on MassARRAY platform is successfully established，which is suitable for HPA typing in Chinese population，and has clinical application prospect.