ADAM15在支气管哮喘表达及意义
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国家自然科学基金(81870039)


The expression and significance of ADAM15 in asthma
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    摘要:

    目的:研究整合素金属蛋白酶15(a disintegrin and metalloproteinase 15,ADAM15)在支气管哮喘中表达及可能机制。方法:收集本院32例哮喘患者及同期16例健康对照的临床资料及血清标本,酶联免疫吸附法检测血清ADAM15水平,分析其与外周血嗜酸性粒细胞数、血清总IgE相关性。构建OVA诱导的过敏性哮喘小鼠模型,免疫组化观察小鼠肺组织ADAM15表达部位和变化。不同细胞因子刺激人支气管上皮细胞系(16HBE),分别用实时定量PCR法和蛋白质免疫印迹法检测细胞ADAM15 mRNA和蛋白表达。结果:与对照组[(398.90±22.13)pg/mL]相比,哮喘患者血清ADAM15水平明显增高[(749.5±132.3)pg/mL,P=0.023],与血清总IgE水平呈正相关(P=0.001 6,r=0.542 5)。ADAM15主要表达于过敏性哮喘小鼠肺组织支气管上皮细胞,其表达量较对照组小鼠升高。IL-13可上调支气管上皮细胞ADAM15 mRNA及蛋白水平。结论:哮喘中ADAM15表达增加,可能与IL-13介导的哮喘气道炎症有关。

    Abstract:

    Objective:This study aims to observe the expression and possible mechanism of a disintegrin and metalloproteinase 15(ADAM15)in asthma. Methods:The clinical data and serum samples of 32 asthma patients and 16 healthy controls were collected,enzyme-linked immunosorbent assay was used to detect the expression of serum ADAM15,and the correlation of peripheral blood eosinophils count and percentage,serum total IgE with ADAM15 was analyzed. A mouse model of OVA-induced allergic asthma was constructed,using immunohistochemistry to observe the expression of ADAM15 expression in lung tissue. Real-time quantitative PCR and Western blotting were used to detect the effect of different cytokines stimulation on the expression of ADAM15 in human bronchial epithelial cell line 16HBE. Results:The level of serum ADAM15 in the asthma group was higher than in the healthy control group[(749.5±132.3)pg/mL vs.(398.90±22.13)pg/mL,P=0.023]. ADAM15 is mainly expressed in bronchial epithelial cells in mouse lung tissue. After 24 hours of stimulation of exogenous IL-13(10 ng/mL),the mRNA and protein levels of ADAM15 were up-regulated. Conclusion:The expression of ADAM15 is elevated in asthma,and may be related with the IL-13-mediated airway inflammation.

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傅 恒,王丽娜,杨月美,郭 帅,姚 欣. ADAM15在支气管哮喘表达及意义[J].南京医科大学学报(自然科学版),2021,(5):684-689

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  • 收稿日期:2021-03-03
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  • 在线发布日期: 2021-06-02
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