The expression and significance of ADAM15 in asthma
Author:
Affiliation:
Fund Project:
摘要
|
图/表
|
访问统计
|
参考文献
|
相似文献
|
引证文献
|
资源附件
|
文章评论
摘要:
目的：研究整合素金属蛋白酶15（a disintegrin and metalloproteinase 15，ADAM15）在支气管哮喘中表达及可能机制。方法：收集本院32例哮喘患者及同期16例健康对照的临床资料及血清标本，酶联免疫吸附法检测血清ADAM15水平，分析其与外周血嗜酸性粒细胞数、血清总IgE相关性。构建OVA诱导的过敏性哮喘小鼠模型，免疫组化观察小鼠肺组织ADAM15表达部位和变化。不同细胞因子刺激人支气管上皮细胞系（16HBE），分别用实时定量PCR法和蛋白质免疫印迹法检测细胞ADAM15 mRNA和蛋白表达。结果：与对照组［（398.90±22.13）pg/mL］相比，哮喘患者血清ADAM15水平明显增高［（749.5±132.3）pg/mL，P=0.023］，与血清总IgE水平呈正相关（P=0.001 6，r=0.542 5）。ADAM15主要表达于过敏性哮喘小鼠肺组织支气管上皮细胞，其表达量较对照组小鼠升高。IL-13可上调支气管上皮细胞ADAM15 mRNA及蛋白水平。结论：哮喘中ADAM15表达增加，可能与IL-13介导的哮喘气道炎症有关。
Abstract:
Objective：This study aims to observe the expression and possible mechanism of a disintegrin and metalloproteinase 15（ADAM15）in asthma. Methods：The clinical data and serum samples of 32 asthma patients and 16 healthy controls were collected，enzyme-linked immunosorbent assay was used to detect the expression of serum ADAM15，and the correlation of peripheral blood eosinophils count and percentage，serum total IgE with ADAM15 was analyzed. A mouse model of OVA-induced allergic asthma was constructed，using immunohistochemistry to observe the expression of ADAM15 expression in lung tissue. Real-time quantitative PCR and Western blotting were used to detect the effect of different cytokines stimulation on the expression of ADAM15 in human bronchial epithelial cell line 16HBE. Results：The level of serum ADAM15 in the asthma group was higher than in the healthy control group［（749.5±132.3）pg/mL vs.（398.90±22.13）pg/mL，P=0.023］. ADAM15 is mainly expressed in bronchial epithelial cells in mouse lung tissue. After 24 hours of stimulation of exogenous IL-13（10 ng/mL），the mRNA and protein levels of ADAM15 were up-regulated. Conclusion：The expression of ADAM15 is elevated in asthma，and may be related with the IL-13-mediated airway inflammation.
