B10细胞在放射性肺纤维化进程中的动态变化
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国家自然科学基金面上项目(81872554);福建省自然科学基金资助项目(2018J01169)


Dynamic changes of B10 cells in the process of radiation⁃induced pulmonary fibrosis
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    摘要:

    目的:探究B10细胞放射性肺纤维化进程中的动态变化。方法:将30只6~8周C57BL/6小鼠随机分为未照射对照组、照射后2 d组(IR2d组)、照射后14 d 组(IR14d组)、照射后3个月组(IR3m组)、照射后5个月组(IR5m组),应用电离辐射建立放射性肺纤维化的动物模型,通过HE染色法、Masson染色观察肺组织的病理变化,免疫荧光染色法观察α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)表达,流式细胞术检测B10细胞在肺、脾脏中的数目变化。结果:胸部照射后早期小鼠肺组织呈现炎性反应;照射后3个月出现肺泡结构破坏,间质填充,照射后5个月大量胶原填充,α-SMA表达较对照组明显增加。IR2d组、IR14d组肺组织中的B10细胞比例较对照组明显增加,而IR3m组B10细胞较IR2d组明显下降;照射后2 d脾脏中B10细胞开始增加,照射后14 d达到峰值,照射后3个月较照射后2 d、14 d明显下降。结论:放射性肺纤维化进程中存在B10细胞浸润,呈现早期升高晚期降低的趋势。

    Abstract:

    Objective:This study aims to explore the dynamic changes in the process of radiation-induced pulmonary fibrosis(RIPF) of B10 cells. Methods:30 C57BL/6 mice,6-8 weeks old,were randomly divided into non-irradiated(control)group,2 days after irradiation(IR2d),14 days after irradiation(IR14d),3 month after irradiation(IR3m)and 5 month after irradiation(IR5m)group.The radiation-induced pulmonary fibrosis model was established by using ionizing radiation. HE staining and Masson staining were used to observe the pathological changes of lung tissue,and the expression of α-SMA was observed by immunohistochemistry. Flow cytometry was used to detect the infiltration changes of B10 cells in lung tissues and spleens in the RIPF model. Results:The lung tissues of mice showed inflammatory response in the early stage after chest irradiation. Furthermore,the alveolar structure destruction and interstitial filling began to appear 3 months after irradiation,and a large amount of collagen was filled 5 months after irradiation. The expression of α-SMA was significantly higher than that of the control group,and pulmonary fibrosis was more severe. The proportion of B10 cells in lung tissue was significantly increased at 2 d and 14 d after chest irradiation compared with the non-irradiated group,while was significantly lower in IR3m group than in IR2d group,B10 cells in the spleens began to increase at IR2d,and reached a peak at IR14d,and was significantly lower in IR3m group than in IR2d group and IR14d group. Conclusion:There were B10 cells infiltration in the process of radioactive pulmonary fibrosis,with a trend of early increase and late decrease.

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潘晓娴,王彩虹,王 锃,陈金梅,蓝瑞隆,陈俊英,张纬建,黄 菲,洪金省. B10细胞在放射性肺纤维化进程中的动态变化[J].南京医科大学学报(自然科学版),2021,(6):804-809

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  • 收稿日期:2020-12-09
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  • 在线发布日期: 2021-06-26
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