Objective：Taking advantage of the PiggyBac transposon system to generate a stable HeLa cell line that can inducibly express Cas9，and to construct a sgRNA library for human nuclear receptor genes to screen for cisplatin resistance genes. Methods： PB-TRE-Cas9-NLS-IRES-hrGFP-Zeo vector was constructed and transferred into HeLa cells together with PB-CAG-rtTA and the transposase vectors. Stable cell lines were obtained by drug screening and picking up single clones. Constructed the sgRNA virus library targeting nuclear receptor genes to infect the stable cell line，screened the clones resistant to cisplatin，and carried out the sequencing and identification of the target genes. Results：Five gene loci were edited efficiently in the stable cell line. Clones resistant to cisplatin were obtained after infection of sgRNA library，and most clones harbored VDR sgRNA and mutant VDR gene. Conclusion：Using PiggyBac transposon system，stable HeLa cell line with inducible expression of Cas9 was successfully constructed. The cell line can be used for efficient gene editing at multiple sites and high-throughput library screening for cisplatin resistance genes.