Objective:This study aims to use bioinformatics methods to screen the differentially long non-coding RNA(LncRNA) in vaginal epithelial tissues of women with vaginal lubrication disorder(LD),and to construct patients with vaginal lubrication disorder based on the hypothesis of lncRNA-mRNA co-expression network to further explore the potential pathogenesis of patients with vaginal LD,in order to provide new ideas for the diagnosis and treatment of patients with vaginal LD. Methods:Using |Log2FC|≥2 and correct P ralue<0.05,the expression profile of long-chain non-coding RNA and mRNA between LD group and normal control group were identified. Using Cytoscape software,a lncRNA-mRNA network of candidate lncRNAs was constructed. Using Gene Ontology(GO)and KEGG Pathway,the biological functions of mRNAs in co-expression network were analyzed. Results:A total of 499 up-regulated lncRNAs,337 down-regulated lncRNAs,and 1 582 up-regulated mRNAs,633 were selected in the LD group and the normal control group based on the next-generation sequencing technology according to the criteria of |Log2FC|≥2 and correct P ralue<0.05. Subsequently,a lncRNA-mRNA co-expression network was constructed based on differentially expressed lncRNA and mRNA through 100 lncRNAs and 311 mRNAs. Finally,the functional enrichment analysis of the lncRNA-mRNA network showed that mRNA was mainly related to myocardial-related diseases and functions. Conclusion:The pathogenesis of LD may be related to blood circulation dysfunction,local muscle dysfunction,and cGMP pathway,etc. This requires further studies at the cellular level,animal level and even human level to confirm.