MicroRNA⁃190调控衰老相关的脂肪能量代谢稳态
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国家自然科学基金(31900841,32071166)


MicroRNA⁃190 regulates aging⁃related fat energy metabolism homeostasis
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    摘要:

    目的:探究微小RNA(microRNA,miR)-190在衰老相关的能量代谢紊乱中的作用和潜在分子机制。方法:利用实时荧光定量PCR(real-time fluorescent quantitative -polymerase chain reaction,RT-PCR),对衰老小鼠脂肪组织中miR-190的表达水平进行检测;构建细胞衰老模型,利用RT-PCR检测脂肪细胞中miR-190的变化;利用RT-PCR检测转染miR-190的脂肪细胞中产热基因的变化;用双荧光素酶报告基因实验验证miR-190的靶基因;利用antagomir注射抑制衰老小鼠脂肪中产生miR-190,检测代谢表征。结果:衰老进程中,脂肪组织中miR-190表达升高;衰老细胞模型中,miR-190的表达同样上调;脂肪细胞中过表达miR-190会导致产热基因的表达降低;产热关键基因PRDM16是miR-190的直接靶基因;抑制衰老小鼠中miR-190的产生,可以有助于改善衰老小鼠的能量代谢稳态。结论:miR-190可能通过靶向PRDM16影响脂肪组织产热,继而影响能量代谢稳态,加速衰老。

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    Objective:This study aims to explore the role and potential molecular mechanism of microRNA(miR)-190 in aging-related energy metabolism disorders. Methods:Real-time fluorescent quantitative PCR(RT-PCR)was used to detect the expression level of miR-190 in the adipose tissue of aging mice;a cellular senescence model was constructed and RT-PCR was used to detect the changes of miR-190 in adipocytes;RT-PCR was used to detect the changes in thermogenesis genes in the adipocytes transfected with miR-190;the target genes of miR-190 were verified by the dual luciferase reporter gene experiment;the metabolic homeostasis was determined in aged mice injected with miR-190 antagomir. Results:During the aging process,the expression of miR-190 in adipose tissue was elevated;in the senescent cell model,the expression of miR-190 was also up-regulated;overexpression of miR-190 in adipocytes would lead to a decrease in the expression of thermogenesis genes;the key genes for thermogenesis PRDM16 is the direct target gene of miR-190;inhibiting the level of miR-190 in aging mice can help improve the energy metabolism homeostasis of aged mice. Conclusion:miR-190 may affect adipose tissue heat production by targeting PRDM16,which in turn affects energy metabolism homeostasis and accelerates aging.

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彭丹丹,李 言. MicroRNA⁃190调控衰老相关的脂肪能量代谢稳态[J].南京医科大学学报(自然科学版),2021,(12):1728-1734

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  • 收稿日期:2021-07-30
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  • 在线发布日期: 2021-12-30
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