血浆外泌体miR⁃409⁃3p的异常表达与冠状动脉搭桥术围术期神经认知障碍的相关性分析
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国家自然科学基金青年项目(82101278,81801061)


Correlation analysis of plasma exosomal miR⁃409⁃3p in perioperative neurocognitive disorders of patients undergoing coronary artery bypass graft surgery
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    摘要:

    目的:分析血浆外泌体miR-409-3p与冠状动脉搭桥术围术期神经认知障碍(perioperative neurocognitive disorders,PND)的相关性。方法:共纳入76 例冠状动脉搭桥术患者,于术前1 d和术后7 d进行神经心理测试评估,并根据评估结果将患者分为PND组(n=24)和非PND组(n=52)。提取所有患者血浆外泌体,分别采用透射电镜、粒径分析、Western blot对其进行鉴定。采用qRT-PCR检测外泌体miR-409-3p的表达水平。Logistic 回归分析PND发生的危险因素。结果:两组在年龄、手术时间及教育年限方面差异有统计学意义。血浆外泌体miR-409-3p在PND组中的表达高于非PND组。年龄、手术时间及外泌体 miR-409-3p为PND 发生的独立危险因素。结论:高表达的血浆外泌体miR-409-3p可作为冠状动脉搭桥术PND的新型分子标志物。

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    Objective:This study aims to analyze the correlation between plasma exosomal miR-409-3p and perioperative neurocognitive disorders (PND) after coronary artery bypass grafting. Methods:A total of 76 patients undergoing coronary artery bypass graft surgery were enrolled in this study. Neuropsychological test assessments were performed 1 day before and 7 days after surgery,and the patients were divided into PND group(n=24) and non-PND group(n=52) according to the evaluation results. The plasma exosomes of all patients were extracted,and were identified by transmission electron microscopy,particle size analysis,and Western blot. The qRT-PCR was used to detect the expression level of exosomal miR-409-3p. Logistic regression analysis was used to evaluate the risk factors for PND. Results:There were statistical differences between the two groups in age,operation time and education years. The expression of plasma exosomal miR-409-3p in PND patients was higher than that in non-PND patients. Age,operation time,and plasma exosomal miR-409-3p were independent risk factors for PND. Conclusion:The highly expressed plasma exosomal miR-409-3p can be used as a new molecular marker for PND in patients undergoing coronary artery bypass graft surgery.

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司林杰,李娜娜,陈艺南,胡柳青.血浆外泌体miR⁃409⁃3p的异常表达与冠状动脉搭桥术围术期神经认知障碍的相关性分析[J].南京医科大学学报(自然科学版),2022,42(3):340-344

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  • 收稿日期:2021-10-15
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  • 在线发布日期: 2022-03-28
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