高频rTMS对健康大鼠认知功能与运动皮质LTP变化的相关性研究
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国家自然科学基金(81672258)


Correlation analysis of cognitive improvement and LTP⁃like plasticity in healthy rats after rTMS
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    摘要:

    目的:探究前额叶高频重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)是否能提高健康大鼠认知功能,以及认知功能与运动皮质长时程增强(long-term potentiation,LTP)样可塑性的相关性。方法:24只SD健康成年大鼠,随机分为control组和rTMS组(n=12),rTMS组接受为期4周的高频(10 Hz)rTMS,control组接受4周假刺激。使用水迷宫检测干预前后认知功能的改变;通过TMS结合肌电图运动诱发电位(motor evoked potential,MEP)评价干预前后运动皮质(M1区)LTP样可塑性的改变;通过Western blot检测干预后大鼠M1区NR1、NR2A、NR2B的蛋白表达改变。结果:前额叶高频rTMS可以提高健康大鼠空间情景记忆能力(P<0.05);前额叶高频rTMS可以增强M1区可塑性,表现为M1区LTP样可塑性增高及NR1和NR2A蛋白表达上调(P<0.05);且M1区LTP样可塑性的改变与空间情景记忆能力成正相关(P < 0.05)。结论:高频rTMS可改善健康大鼠的空间情景记忆及运动皮质LTP样可塑性,且认知功能与运动皮质LTP样可塑性呈正相关。

    Abstract:

    Objective:This study aims to investigate the contribution of M1 long-term potentiation(LTP)-like plasticity to the improvement of cognitive function by high frequency repetitive transcranial magnetic stimulation at healthy rat’s head. Methods:A total of 24 rats were randomly divided into control group and rTMS group(n=12). The cognitive function was detected by Morris water maze. The M1 plasticity was reflected by the motor-evoked potential(MEP) before and after rTMS on the primary cortex(M1),the protein expressions of NR1,NR2A and NR2B in the M1 region of rats after high frequency rTMS were detected by Western blot. Results:After treatment,the spatial episodic memory and M1 plasticity showed significant improvement versus the control group(P<0.05). High-frequency rTMS in the prefrontal cortex can enhance the plasticity of the M1 region,which is characterized by increased LTP-like plasticity and up-regulated NR1 and NR2A protein expression in the M1 region. And the changes of LTP-like plasticity in M1 region were positively correlated with spatial episodic memory ability (P<0.05). Conclusion:High-frequency rTMS could improve spatial episodic memory and cortical LTP-like plasticity in healthy rats,and the improvement in spatial contextual memory was positively correlated with motor cortical plasticity.

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徐兴俊,沈 滢,伍 琦,朱 奕,郭 川,戴文骏,章天娇,王 彤.高频rTMS对健康大鼠认知功能与运动皮质LTP变化的相关性研究[J].南京医科大学学报(自然科学版),2022,42(5):632-637

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  • 收稿日期:2021-12-03
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  • 在线发布日期: 2022-05-23
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