兰索拉唑诱导心肌细胞氧化应激损伤的机制研究
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国家自然科学基金(81673515);江苏省自然科学基金(BK20161591)


Mechanism of lansoprazole inducing oxidative stress injury in myocardial cells
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    摘要:

    目的:探索兰索拉唑(lansoprazole,LPZ)对大鼠心肌细胞H9C2的潜在损伤效应及相关机制。方法:以10 μmol/L LPZ孵育H9C2细胞不同时间(0 h、12 h、24 h、48 h)后,采用DCFH-DA荧光探针检测细胞内活性氧(reactive oxygen species,ROS)水平,Western blot检测内质网应激蛋白(GRP78、CHOP)及凋亡相关蛋白(Cleaved-Caspase-12、Cleaved-Caspase-3、Cyt C、Bax/Bcl-2)的表达。此外,兰索拉唑及ROS抑制剂N-乙酰-L-半胱氨酸(N-acetyl-L-cysteine,NAC)单独或联合孵育细胞48 h后,检测H9C2细胞内ROS水平,以及内质网应激和凋亡相关蛋白的表达情况。结果:LPZ可以时间依赖性增加ROS的水平,诱导GRP78和CHOP的表达,并进一步增加Cleaved-Caspase-12、Cleaved-Caspase-3、Cyt C、Bax/Bcl-2的表达。NAC显著降低LPZ诱导的ROS水平,降低GRP78、CHOP以及Cleaved-Caspase-12、Cleaved-Caspase-3、Cyt C、Bax/Bcl-2的表达水平。结论:LPZ可诱导心肌细胞凋亡,机制可能与氧化应激和内质网应激有关。

    Abstract:

    Objective:This study aims to explore the potential damage effect and related mechanisms of lansoprazole(LPZ)on rat cardiomyocytes(H9C2). Methods:After incubating H9C2 cells with 10 μmol/L LPZ for 0 h,12 h,24 hand 48 h,DCFH-DA fluorescent probe was used to detect the level of reactive oxygen species(ROS). And the expression of endoplasmic reticulum stressproteins(GRP78,CHOP)and apoptosis-related proteins(Cleaved-Caspase-12,Cleaved-Caspase-3,Cyt C,Bax/Bcl-2)was detected by Western blot. In addition,the level of ROS,as well as the expression of endoplasmic reticulum stress and apoptosis-related proteins were evaluated after H9C2 cells incubating with LPZ alone or in combination with ROS inhibitor N-Acetyl-L-cysteine(NAC)for 48 h. Results:Lansoprazole could time-dependently increase the level of ROS,induce the expression of GRP78 and CHOP,and further increase the expression of Cleaved-Caspase-12,Cleaved-Caspase-3,Cyt C,and Bax/Bcl-2. NAC significantly reduced LPZ-induced ROS levels,and decreased the expression levels of GRP78,CHOP,Cleaved-Caspase-12,Cleaved-Caspase-3,Cyt C and Bax/Bcl-2. Conclusion:LPZ could induce cardiomyocyte apoptosis,and the mechanism may be related to oxidative stress and endoplasmic reticulum stress.

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钱徐萍,张学会,孙鲁宁,王 雨,孙诗钰,马梦圆,程紫萍,陈安九,王永庆.兰索拉唑诱导心肌细胞氧化应激损伤的机制研究[J].南京医科大学学报(自然科学版),2022,42(6):802-808

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  • 收稿日期:2022-02-01
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  • 在线发布日期: 2022-06-21
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