小鼠不同脂肪组织对冷应激的适应性反应
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国家自然科学基金(82070849);江苏省“双创团队”项目


The adaptive responses of different adipose tissues to cold stress in mice
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    摘要:

    目的:明确C57BL/6J小鼠不同脂肪组织对于冷刺激适应性应答的相似点与差异。方法:建立冷刺激组及对照组小鼠模型,测量体重、进食量等;HE染色观察各脂肪组织切片形态;荧光定量PCR法检测棕色化、线粒体功能等相关基因的表达变化;Western blot检测产热等相关蛋白的表达情况;免疫组化检测脂肪组织交感神经支配;流式细胞术分析脂肪组织中巨噬细胞的分型。结果:与米色和白色脂肪相比,棕色脂肪组织的产热相关基因解偶联蛋白1表达量最高,但冷刺激不能进一步促进其表达;而冷刺激能够显著诱导米色脂肪组织中产热基因和蛋白的表达。冷刺激能够促进棕色脂肪和米色脂肪中交感神经支配和巨噬细胞M2型极化。结论:冷刺激能够诱导米色脂肪组织棕色化,同时促进交感神经支配和巨噬细胞的M2型极化;冷刺激对内脏白色脂肪组织的影响最小。

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    Objective:This study aims to clarify the similarities and differences of adaptive responses of different adipose tissues in C57BL/6J mice to cold stress. Methods:Mice were divided into two groups,the control group which were housed under room temperature individually and the cold stress group were housed under 6 ℃. Body weight,food intake and adipose tissue weight were measured. Adipose tissue morphology were observed by HE staining. The genes related to browning,mitochondrial function were quantified by qRT-PCR. The proteins related to thermogenesis were detected by Western blot. Adipose tissue sympathetic innervation was shown by IHC staining. Macrophages polarization were tested by flow cytometry. Results:Thermogenic gene Ucp-1 expression were most abundant in brown adipose tissues but could not be promoted by cold. By contrast,thermogenic gene expression could be stimulated by cold stress in beige adipose tissue in both mRNA and protein levels. Simultaneously,sympathetic innervation and macrophages M2 polarization could be induced in both brown and beige adipose tissue. Conclusion:Cold stress could promote sympathetic innervation and macrophage M2 polarization in beige adipose tissue which could induced browning. And cold stimulation has minimal role in regulating visceral white adipose tissues regarding themogenic gene expression and macrophage polarization.

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耿 蓉,罗 岩,孙 翠,向李倩,刘 煜.小鼠不同脂肪组织对冷应激的适应性反应[J].南京医科大学学报(自然科学版),2022,42(6):809-814

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  • 收稿日期:2022-02-11
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  • 在线发布日期: 2022-06-21
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