Objective: To observe the differences of partial physiologic indexes including weight, food intake,Lee's index, the wet weight of fat pad, plasma lipids and the genetic expression of LPL mRNA in adipose tissue between obesity-prone rats (OP) and obesity-resistant rats (OR) on a high-fat diet. Methods: After 1 week of free access to a high-fat diet (HFD), 48 rats were separated on the basis of 1 week body weight percentage gained in OP (OP≥P75)or OR ( OR≤ P25) groups. Rats were continuously fed on the HFD for another 4 weeks. The body weight and food intake were recorded in the course of model-making. And the Lee's index, the plasma lipid and lipoproteius, the wet weight of both epididymal and retroperitoneal fat pad were measured after the rat was killed. And the level of LPL mRNA exgain, the cumulative energy intake, the Lee' s index, and the wet weight of fat pad at both epididymal and retroperitoneal sites were significantly higher than those in OR rats, but there was no significant difference in the level of plasma weight in OP rats were about 6.45 and 4.25 times of those in OR rats. Meanwhile, the cumulative energy intake in OP level of LPL mRNA expression in adipose tissue, there was a significant level of LPL mRNA in adipose tissue of OP rats compared with that in OR rats. Conclusion: The physiologic differences exist between OP and OR rats. Besides a higher level of energy intake, the higher energy efficiency associated with LPL mRNA expression in adipose tissue may also contribute to the enhancement of susceptibility to obesity in OP rats.