Objective: Previous studies have shown that glioma cells induced thc migration of nerve stem cells (NSCs)in vivo. This study was exploring whether this situation could happen in vitro. Methods: Supematant from C6 glioma cell lines or astrocytes cultured in serum-free medium growing in logarithmic phase were separately placed in lower chambers and NSCs were placed in the upper chambers of Trans-well culture system. After 36 h co-incubation, these NSCs spheres occurred on the middle membrane were counted. Results: Results demonstrated that the supernatant from C6 glioma cell culture, not from the astrocytes, enhanced the migration of NSCs ( P ＜0.01). Conclusion: Some components in C6 glioma cell culture can atract the migration of NSCs.