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Effects of different dose endothelin-1 on expession of peroxisome proliferator-γ in cultured vascular smooth muscle cells of adult rats
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    Objective: To investigate the effects of endothlin- 1 (ET- 1 ) on vascular smooth muscle cells(VSMCs ) proliferation and the expression of Peroxisome proliferator-activated receptorγ (PPAR-γ) in VSMCs. Methods: VSMCs of 16-week-old wistar rats thoracic aorta were cultured. VSMCs were treated by ET-1 for 48 h and observed of the proliferation by MTT. The expression of PPAR-γmRNA and protein in cultured VSMCs treated by different concentration of ET-1 for 48 h was detected by RT-PCR and Western blot.Results: Compared with control group, VSMCs treated by ET-1 proliferated with the increase concentration of ET-1. There was significant differences among different groups ( P < 0.01). Meanwhile, the expression of PPAR-γ both in mRNA and in protein levels deceased. The expression of PPAR-γ in VSMCs was gradually decreased along with the increase concentration of ET-1. There was significant differences among different groups ( P <0.01). Compared with control group, the expression of both PPAR-γ mRNA and PPAR-γ in ET-1 treated groups were lower( P < 0.01). Conclusion: ET-1 could induce VSMCs proliferation and the expression of PPAR-γ in VSMCs, which demonstrates that high dose ET-1 obviously weakens the function of PPAR-γ to increase VSMCs proliferation.

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Zhengli Di, Xiaolin Niu, Jin Wei, Nanping Wang.[J].南京医科大学学报(自然科学版),2005,19(6):

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