Objective: To observe the effects of Bushen Zhuanggu Formul on interleukin 6 (IL-6) level and bone metabolism of the ovariectomized rats, and to explore the possible mechanism of preventing and treating postmenopausal osteo porosis by traditional Chinese Formul. Methods: SD rats were randomly divided into 4 groups: normal control group (N),ovariectomized control group (OVX), estrogen-treated group (OVX+E) traditional and Chinese medicine-treated group (OVX+CM). After treatment, IL-6 levels in rat bone tissue media and serum were examined by ELISA. The proximal tibias(PTM) of rats were undecalcified for quantitative bone histomorphometry. Results: Compared with OVX group, both Bushen Zhuanggu medicine and estrogen decreased IL-6 level in rat bone tissue media. However, the effects of Bushen Zhuanggu Formul were weaker than those of estrogen. Furthermore, Bushen Zhuanggu Formul and estrogen produced a reduction in bone turnover by inhibiting bone formation and bone resorption at the same time. Conclusion: Bushen Zhuanggu Formul prevents ovariectomic bone loss by inhibiting IL-6 production in rat bone tissues.