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Over-expression of hypoxia-inducible factor 1 alpha increases angiogenesis of LNCaP cells
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    Objective:To evaluate the effect of HIF-1 α over-expression on angiogenesis in human prostate cancer cells. Methods:LNCaP cells(a human prostate cancer cell line) were transfected with the recombinant plasmid pcDNA3.1(-)-HIF-1α with Lipofectamine 2000 system. The positive clones were selected by G418 being further confirmed by Western blot and immunofluorescence. The expression levels of VEGF, iNOS and Ang-Ⅱ were determined. Results:The expression of HIF-1α in the LNCaP/HIF1α cells was significantly increased in transfected cells, which induced the up-regulation of VEGF, iNOS, whereas Ang-Ⅱ expression remained un- changed. Conclusion:Over-expression of HIF-1α can induce angiogenesis proteins and may improve the angiogenesis potency of prostate cancer.

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Yili Han, Dalin He, Yong Luo, Hepeng Cheng, Guangfeng Zhu.[J].南京医科大学学报(自然科学版),2007,(4):253-256

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  • 收稿日期:2007-02-26
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