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Selection of DNA aptamer that specific binding human carcinoembryonic antigen in vitro
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This work was supported by the China National Natural Science Foundation(30670958)

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    Objective:To select the specific aptamer of carcinoembryonic antigen (CEA), one of the most attractive molecule for cancer target therapy and imaging. Methods: Seven rounds in vitro selection were performed against the purified CEA protein. Ligand-mediated target purification and Co-immunoprecipitation were adopted to verify the specific binding of the aptamer to the purified and native protein separately. Results:The CEA-specific aptamer which can bind both the purified and native protein with the high specificity was obtained. Conclusion:This is the first time the CEA specific apatmer was produced. The results in this study provides the preliminary evidence for further investigation and application of CEA-aptamer in the future.

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Lifeng Wang, Baorui Liu, Haitao Yin, Jia Wei, Xiaoping Qian, Lixia Yu.[J].南京医科大学学报(自然科学版),2007,(5):277-281

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  • 收稿日期:2007-06-06
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