Objective: To study the effect of ischemia-reperfusion injury(IRI) and hepatic artery ischemia(HAI) on CD14 expression in canine auto-transplantation livers. Methods:Liver orthotopic auto-transplantation models were applied with 30 healthy male Xi’an canines which were randomly divided into a control group, simultaneous reperfusion(SR) group and HAI group. CD14 protein expression, Malonaldehyde (MDA) Contents in hepatic tissues and ALT values in serum were detected, and the pathological changes of hepatic tissues was investigated under the light microscopy. Results:The level of CD14 protein expression in SR and HAI group tended to be time-dependent and both higher than controls with statistical significance(P ＜0.01); The peak values of these two groups both occurred at 4 h, but the level in HAI group (11.94±0.43) was evidently higher than that in SR group(3.04±0.34). MDA contents in liver tissue, ALT values in serum and pathological changes showed the same changing tendency as CD14 expression. Conclusion:①Up-regula-tion of CD14 expression may be the receptor-mechanism of Kupffer cells(KCs) activation in liver transplantation. ②HAI can up-regulate CD14 expression after portal vein reperfusion, improve the activity of KCs further more, increase OFRs production and coop-erate with portal reperfusion, and finally aggravate the grafts injury.