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Effects of Oxymatrine on the NF-kappa B expression of HaCaT cells
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    Abstract:

    Objective: To study the effect of oxymatrine on the expression of nuclear factor-kappa B(NF-κB) of Human benign epidermal keratinocytes line(HaCaT cells). Methods:HaCaT cells were cultured with different concentration of Oxymatrine(10μg/ml, 50μg/ml and 100μg/ml) for 24 h, then 10-5 mol/L Substance P was added to the cells. After 30 min, NF-κB expression in the cells was observed by immunocytochemistry, NF-κB P65 protein expression was evaluated by Western blot, and the mRNA expression of NF-κB P65 was evaluated by reverse transcription polymerase chain reaction(RT-PCR). The 10-5 mol/L Substance P and culture medium were added to the Substance P group and control group, respectively. Results:In control group, expression rate of positive cells, the expressions of protein and mRNA of NF-κB were all low. In Substance P group, when 10-5 mol/L Substance P was added, the expres-sions were all increased(P < 0.05). But in Oxymatrine groups, the expression rate of positive cells, the expressions of protein and mRNA were all descended in a concentration-dependent manner(P < 0.05 or P < 0.01). Conclusion:Oxymatrine can down-regulate the expression of NF-κB of the HaCaT cells and may play an important role in regulating anti-inflammation and immunity.

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Caiqing Zhang, Jie Feng, Xiaoning Yan, Wenbing Li, Gang Hu.[J].南京医科大学学报(自然科学版),2008,28(3):178-182

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  • 收稿日期:2007-11-14
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