Objective: To explore influences of CHD4 gene expression on proliferation and apoptosis of acute T lymphoblastic leukemia cells and to identify its promoter. Methods: qRT-PCR and Western Blot analysis were used to verify transfection efficiency of siRNA-CHD4. CCK-8 assay and Flow Cytometry assay were adopted to detect the influence of expression of CHD4 on apoptosis and proliferation in Jurkat cells. PCR, DNA ligase and E.coli were used to clone the promoter region of CHD4 gene into pGL3-Basic to construct luciferase reporter plasmids. By means of transient transfection and dual-luciferase reporter assay, we analyzed the promoter activity of CHD4. Result: Flow cytometry showed that, compared with the control group, CHD4 inhibited the apoptosis of Jurkat cells, the Jurkat cells transfected siRNA-CHD4 were significantly increased in the G0/G1 phase and decreased in the S phase (7%-10%) (P < 0.01), CCk-8 essay identified that CHD4 gene promoted the proliferation of Jurkat cells (P < 0.05). Plasmids containing CHD4 gene candidate promoter and truncated sequence plasmids were successfully constructed. Compared with empty vector, plasmids containing CHD4 gene candidate promoter sequences were significantly more active (P < 0.05).The core promoter of CHD4 gene located in 233 bp to 13 bp relative to transcription start site, which contained the NF-?B ,MZF1 transcription factor binding sites, NF-?B had positive function to the CHD4 promoter activity. Conclusion: Our results suggested that CHD4 inhibited apoptosis and induced proliferation in Jurkat cells. The core promoter of CHD4 located in -233/-13bp relative to TSS, NF-?B bound to the core promoter of CHD4 in vivo and it had positive function to the promoter activity of CHD4.