Abstract:Objective: A soothing liposome emulsion was developed by selecting Madecassoside and Ectoin as the primary ingredients. The transdermal absorption of the emulsion was subsequently evaluated. Method: The optimal ratio of Madecassoside and Ectoin, capable of eliciting anti-inflammatory and barrier repair activities, was identified through cellular assays. The preparation of hydroxy Madecassoside-Ectoin liposomes was conducted utilizing thin film hydration; subsequent characterization involved the determination of particle size, dispersibility coefficient, and charge using the Malvern particle size analyzer.The morphology was observed under projective electron microscope.The contents of Madecassoside and Ectoin in solution were determined by high performance liquid chromatography. The encapsulation rate and drug loading were determined by dialysis method. The transdermal characteristics of the liposome emulsion were evaluated via the Franz diffusion cell method. Results: A mass ratio of 1:9 for Madecassoside to Ectoin demonstrated optimal anti-inflammatory and barrier repair capabilities. Under these parameters, the liposome preparation conditions were optimized with a phosphorus-bile ratio of 5:1 and a drug-to-lipid ratio of 1:10.The encapsulation rate of Madecassoside in the liposomes was 51.33%, the drug loading capacity of Madecassoside was 0.35%, the encapsulation rate of Ectoin was 26.39% and the drug loading capacity of Ectoin was 1.2%. The particle size is 166.53 nm, the potential is -29.63 mV, and the PDI is 0.2.Over a 24 hour period, the solution demonstrated a lower cumulative permeability compared to the aqueous control, without exhibiting an abrupt release; instead, a sustained release effect was observed. Conclusion: The liposome emulsion prepared in this experiment has a high drug load and stable internal particle distribution, and can achieve slow-release effect on the skin surface and prolong the action time of the effective substance.