• 2007年第1期文章目次
    全 选
    显示方式: |
    • >南京医科大学学报(自然科学版)
    • bFGF对成骨细胞整合素β1亚单位表达和细胞黏附的影响

      2007(1):1-5. DOI: 10.7655

      摘要 (1913) HTML (34) PDF 0.00 Byte (97) 评论 (0) 收藏

      摘要:目的:研究碱性成纤维细胞生长因子(bFGF)对成骨细胞整合素β1亚单位表达的影响,探讨纤维黏连蛋白(FN)与碱性成纤维细胞生长因子(bFGF)单独或联合应用时对成骨细胞在生物衍生骨支架上黏附的影响。方法:培养成骨细胞,以bFGF(100 ng/ml)刺激,接种于FN(10 μg/ml)修饰的生物衍生骨支架上,Western blot检测bFGF对FN受体整合素β1亚单位表达的影响, MTT检测组间细胞黏附效率差异,电镜观察细胞密度与形态。结果:Western blot检测发现应用bFGF刺激后,成骨细胞整合素β1亚单位表达增强;MTT检测发现单独应用FN与bFGF均可增加细胞在生物衍生骨上的黏附效率(P < 0.01),但二者联合应用时,产生的效应进一步增强,二者存在统计学上的交互作用(P < 0.01);电镜观察也证明,二者联合应用时细胞密度、铺展效果均优于单独应用。结论:bFGF可以显著增强成骨细胞FN受体整合素β1亚单位的表达,明显提高成骨细胞在FN修饰生物衍生骨支架上的黏附效率,bFGF与FN对于促进成骨细胞的黏附具有交互作用。

    • >南京医科大学学报(英文版)
    • Role of intraoperative transesophageal echocardiography in coronary artery bypass grafting

      2007(1):1-7. DOI: 10.7655

      摘要 (1780) HTML (40) PDF 1.69 M (2255) 评论 (0) 收藏

      摘要:Transesophapeal echocardiography (TEE) can be used as a diagnostic tool during cardiac surgery to direct the surgical procedure and diagnose unanticipated problems. TEE has also been one of the most important means of monitoring myocardial ischemia during coronary artery bypas grafting procedures. The cardiac anesthesiologist can apply intraoperative TEE in evaluating coronary artery anatomy and aorta atherosclerosis,assessing diastolic left ventricular function and preload,measuring intracardiac pressure and cardiac output,detecting ischaemic mitral regurgitation,intracardiac air and pericardial effusion.

    • >南京医科大学学报(社会科学版)
    • 医生收受回扣的行为不构成受贿罪——与裴显鼎先生商榷

      2007(1):5-9. DOI: 10.7655

      摘要 (1730) HTML (40) PDF 0.00 Byte (82) 评论 (0) 收藏

      摘要:十届全国人大常委会第22次会议通过了《刑法修正案(六)》,其中第7条对刑法第163条作了修改:将公司企业人员受贿罪的主体扩大解释为“公司、企业或者其他单位的工作人员”。针对裴显鼎先生关于“这一修正案的施行,为国有医院的普通医生收受回扣的行为按刑法第163条的规定,定企业人员受贿罪提供了法律依据”的解释,笔者认为,普通医生收受药品回扣的行为,既不能构成受贿罪,也不构成企业人员受贿罪;裴显鼎先生的观点犯了偷换概念的错误,是自相矛盾的,违背了我国刑法罪刑法定和不适用类推的原则。

    • >南京医科大学学报(自然科学版)
    • 11β-羟化类固醇脱氢酶1促进3T3-L1前脂肪细胞分化

      2007(1):6-10. DOI: 10.7655

      摘要 (2298) HTML (42) PDF 0.00 Byte (165) 评论 (0) 收藏

      摘要:目的: 应用3T3-L1细胞模型研究11β-羟化类固醇脱氢酶1(11β-HSD1)与前脂肪细胞分化的关系,探讨11β-HSD1在前脂肪细胞分化及肥胖中的作用。方法:构建11β-HSD1-SiRNA 表达质粒pGCsilencerTM H1/TetO1-11β-HSD1并稳定转染3T3-L1细胞,采用油红染色观察脂滴堆积情况,采用Western blot方法检测11β-HSD1在正常3T3-L1细胞分化过程中的表达;并用Real-time PCR检测脂肪细胞分化相关标志基因的变化,阐明11β-HSD1 对前脂肪细胞成脂分化的影响。结果:正常3T3-L1前脂肪细胞在上述三联诱导分化后脂滴堆积随着分化过程逐渐增加;在pGCsilencerTM H1/TetO1-11β-HSD1转染的3T3-L1诱导分化过程中可见脂滴堆积的减少。在3T3-L1前脂肪细胞分化模型中(days 0,2,4,6,8),11β-HSD1蛋白水平是上调的。3T3-L1前脂肪细胞分化模型中GR在分化模型早期(D4及D6)是上调的,但是在分化后期(D8)很快又下降。在正常3T3-L1前脂肪细胞分化早期LPL上调,随着分化后期FAS、PPARγ等明显上升,Pref-1作为脂肪细胞分化抑制因子,在分化早期升高,在分化后期明显下调。在pGCsilencerTM H1/TetO1-11β-HSD1转染后的3T3-L1细胞分化模型中上述有关标志基因发生变化。结论: 11β-HSD1 作为受体前调节剂促进前脂肪细胞分化,与肥胖和胰岛素抵抗相关。

    • >南京医科大学学报(英文版)
    • Establishment of simultaneous pancreas and kidney transplantation (SPK) model with cuff technique and portal venous drainage in rats

      2007(1):8-10. DOI: 10.7655

      摘要 (1646) HTML (40) PDF 77.38 K (2196) 评论 (0) 收藏

      摘要:Objective: To establish a simultaneous pancreas and kidney transplantation (SPK) model in the rat. Methods: SD rats served as donors and recipients. The donor portal vein and the recipient superior mesenteric vein were anastomosed and the donor renal veins and recipient renal veins were anastomosed by cuff method. Arterial reconstruction was carried out by end to side anastomosis of the donor abdominal aorta to the recipient abdominal aorta. Enteric drainage was performed by side to side anastomosis between donors’ duodenum and recipients’ jejunum. The donor ureter -bladder valve was anastomosed to the bladder of recipients. Results: Out of 30 cases of SPK transplantation, 24 had normal serum glucose and serum creatinine after operation. The successful rate was 80 %. Conclusion: This model of SPK in rats is stable and reliable, which could be applied for further scientific research.

    • >南京医科大学学报(社会科学版)
    • 建议设立“医务人员受贿罪”

      2007(1):10-12. DOI: 10.7655

      摘要 (1697) HTML (38) PDF 0.00 Byte (100) 评论 (0) 收藏

      摘要:面对不断突出的医务人员收受回扣的行为,当前立法却出现了空白缺失,理论上对于是否需要以及如何进行刑法规制存在争议,实践中做法也甚为混乱。要研究和解决这一问题,必须科学合理地分析医务人员收受回扣行为的特殊性及其与国家工作人员受贿行为,公司、企业或者其他单位人员受贿行为的区别,正视这种差异,通过设立独立的新的罪名来实现对医务人员收受回扣行为的有效打击。

    • >南京医科大学学报(英文版)
    • Expression of 8-hydroxy-2’-deoxyguanosine in gastric carcinomas

      2007(1):11-14. DOI: 10.7655

      摘要 (1727) HTML (36) PDF 1019.28 K (2064) 评论 (0) 收藏

      摘要:Objective: Reactive oxygen species may be involved in the progression of gastric carcinomas. To clarify whether the pathology of gastric carcinoma are related to oxidative DNA damage, the expression of 8-hydroxy-2’-deoxyguanosine (8-OHdG) was examined in 30 patients with gastric carcinomas. Methods: The expression of 8-OHdG and apoptosis in the gastric carcinoma were measured using the methods of immunocytochemistry and deoxynucleartididyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), respectively. Results: Of the 30 cases, 25(83%) showed stronger immunoreactivity than normal control. The patients with poorly differentiated gastric carcinoma had a larger tumor size and higher labeling indices of TUNEL- and 8-OHdG-positive cells than those with well and moderately differentiated gastric carcinoma. Conclusion: Our findings suggest that oxidative DNA damage is increased in association with necroinflammation in chronic gastric injuries and determination of 8-OHdG is useful in assessing high-grade malignancy in gastric carcinomas.

    • >南京医科大学学报(自然科学版)
    • 腹主动脉灌注法分离胰腺星状细胞及其培养、鉴定

      2007(1):11-14. DOI: 10.7655

      摘要 (2344) HTML (36) PDF 0.00 Byte (104) 评论 (0) 收藏

      摘要:目的:通过腹主动脉插管灌注胶原酶分离胰腺星状细胞(pancreatic stellate cell, PSC),建立简单易行的PSC分离方法,并对PSC进行培养和鉴定。方法:大鼠腹主动脉插管灌注后,经胶原酶消化、Nycodenz密度梯度离心,分离得到PSC;通过观察细胞形态、胞质内脂滴和免疫细胞化学方法检测结蛋白(desmin)、神经胶质酸纤维蛋白(glial fibrillary acidic protein,GFAP)、α平滑肌肌动素(α-smooth muscle actin,α-SMA)的表达来鉴定PSC。结果: 腹主动脉灌注法分离得到PSC,产率、活力和纯度分别为:(15.3 ± 4.6) × 103/g体重﹑(95.0 ± 3.5)%﹑﹥80%;培养24 h后大多数细胞已贴壁,呈星状或多角形,48 h后表达desmin、GFAP,并在96 h 后表达α-SMA。结论:通过腹主动脉灌注法较容易分离得到PSC,其产率、活力和纯度较高,经鉴定后能够满足体外实验要求。

    • AQP4参与卵巢激素对单胺类神经递质的调节作用

      2007(1):15-18. DOI: 10.7655

      摘要 (2143) HTML (52) PDF 0.00 Byte (89) 评论 (0) 收藏

      摘要:目的:探讨AQP4对卵巢激素调节神经递质作用的影响。方法:应用雌性AQP4基因敲除型CD1小鼠与野生型CD1小鼠,测定AQP4基因敲除对小鼠血浆中雌孕激素水平的影响;两种基因型小鼠实施卵巢去势手术(OVX),测定纹状体和皮层中单胺类神经递质的含量。结果:AQP4基因敲除型小鼠动情后期血浆雌、孕激素水平显著低于野生型小鼠;去势后野生型小鼠纹状体多巴胺(DA)及皮层去甲肾上腺素(NE)含量明显降低,但去势不影响AQP4基因敲除型小鼠脑内相应的递质水平。结论:AQP4参与了卵巢激素对单胺类神经递质的调节。

    • >南京医科大学学报(英文版)
    • Critical visceral adipose tissue thresholds aassociated with type 2 diabetes mellitus in chinese population

      2007(1):15-20. DOI: 10.7655

      摘要 (1619) HTML (40) PDF 394.35 K (2075) 评论 (0) 收藏

      摘要:Objective: To compare intervertebral location L2-L3 with L4-L5 as landmarks for measuring abdominal fat distribution and to determine critical levels of visceral adipose tissue(VAT) at those planes, exceeding which may lead to the development of type 2 diabetes. Methods: Abdominal fat distribution was measured using computed tomography(CT) in 29 diabetics (19 male, 10 female) and 30 non-diabetics (18 male, 12 female). CT images obtained at two intervertebral locations L2-L3 and L4-L5 were used to measure the areas of total fat, VAT and subcutaneous adipose tissue(SCAT) using slice thickness of 5mm and an attenuation range from -190 to -30 Hounsfield units(HU). Data were analyzed using logistic regression and Receiver-operating characteristic(ROC) analysis. Results: At L2-L3, diabetes and obesity were correctly classified at 91.53% and 83.05% respectively, while at L4-L5, the same were correctly classified at 84.75% and 88.14% respectively. VAT compared to SCAT, had significantly higher correctly classified percent values for predicting diabetes in both measurement sites. At L2-L3, VAT≥177.29 cm2 or VAT≥51.52% of the total fat area had the highest correctly classified value for predicting diabetes in men, while VAT≥132.27 cm2 or VAT≥45.7% of the total fat area had the highest correctly classified value for predicting diabetes in women. At L4-L5, VAT≥130.82 cm2 or VAT≥45.54% of the total fat area had the highest correctly classified value for predicting diabetes in men, while VAT≥118.56 cm2 or VAT≥32.24% of the total fat area had the highest correctly classified value for predicting diabetes in women. Conclusion: L2-L3 plane is a better landmark for measuring abdominal fat distribution for predicting diabetes, while abdominal fat distribution measured at L4-L5 has better association with obesity. Regardless of the measurement site, VAT compared to SCAT, has significantly stronger association with diabetes.

    • >南京医科大学学报(社会科学版)
    • 非法行医罪客观方面若干问题探讨

      2007(1):15-17. DOI: 10.7655

      摘要 (1554) HTML (41) PDF 0.00 Byte (79) 评论 (0) 收藏

      摘要:非法行医罪是职业犯,因而“偶尔为之”者不应当认为是犯罪;行医之“医”是具有专业性和危险性的预防、治疗疾病的活动,巫术、饮食疗法等活动不是非法行医。此外,文章还探讨了非法行医罪构成要件中“情节严重”的若干情形。

    • 浅析医疗行为中患者有效同意的法律要件

      2007(1):18-21. DOI: 10.7655

      摘要 (1844) HTML (38) PDF 0.00 Byte (87) 评论 (0) 收藏

      摘要:在医疗行为中,医生充分的说明、患者具备同意能力,且自主自愿的同意是患者有效同意的法律要件。在医疗行为后,患者常以其同意无效为由,要求损害赔偿。因此,在医疗过程中,医方既要尊重患者的自己决定权,加强医患之间的沟通,又要规范自己的操作,加强自我保护意识。

    • >南京医科大学学报(自然科学版)
    • RhoA-ROCK信号通路介导缺氧所致乳腺癌细胞肌动蛋白细胞骨架重构

      2007(1):19-22. DOI: 10.7655

      摘要 (2322) HTML (40) PDF 0.00 Byte (158) 评论 (0) 收藏

      摘要:目的:探讨缺氧对乳腺癌细胞(MDA-MB-435细胞)肌动蛋白细胞骨架重构和细胞形态改变与RhoA-ROCK信号通路的关系。方法:对体外培养的MDA-MB-435细胞进行缺氧16 h处理,或在缺氧前用ROCK的特异性抑制剂Y-27632(10 μmol/L)预处理细胞1 h,再进行缺氧处理。用共聚焦激光扫描显微镜研究缺氧或使用Y-27632对MDA-MB-435细胞肌动蛋白细胞骨架重构的影响和细胞形态的改变。结果:乳腺癌细胞经16 h缺氧处理,肌动蛋白细胞骨架发生解聚,应力纤维的排列和分布以及细胞形态明显改变;事先使用Y-27632再进行缺氧处理,可防止缺氧所致乳腺癌细胞内肌动蛋白细胞骨架解聚,同时应力纤维在细胞内排列和分布恢复正常。结论:RhoA-ROCK信号通路可能参与缺氧所致乳腺癌细胞内肌动蛋白细胞骨架的重构和细胞形态的改变。

    • >南京医科大学学报(英文版)
    • Effect of NK cells on GVHD in H-2 haploidentical bone marrow transplantation in mice

      2007(1):21-24. DOI: 10.7655

      摘要 (1697) HTML (44) PDF 1.20 M (2049) 评论 (0) 收藏

      摘要:Objective: To study the effect of natural killer (NK) cells on graft-versus-host disease (GVHD) after H-2 haploidentical bone marrow transplantation(BMT) in mice. Methods:Murine model of H-2 haploidentical BMT was established by using Balb/c(H-2d) mouse as recipient, and Balb/c(H-2d)×C57BL/6(H-2b)(H-2d/b) mouse as donor. Lethally irradiated Balb/c(H-2d) mice were transplanted with the bone marrow cells from Balb/c(H-2d)×C57BL/6(H-2b)(H-2d/b) mice containing donor spleen cells and/or NK cells. GVHD and survival rates were studied by observation of clinical manifestations and pathological changes. Results:In the group of bone marrow +spleen cells, GVHD was induced in 90% mice; but in the group plus with low amount of NK cells, GVHD was induced in 20% mice; and in the group transplanted with high amount of NK cells, GVHD was induced only in 10% mice. Compared to the group transplanted only with BM plus spleen cells, the incidences of GVHD in the latter two groups decreased significantly(P < 0.01) and the survival rates at different periods of 15, 30, 45 and 60 days increased obviously(P < 0.01). Conclusion: In mouse H-2 haploidentical BMT, alloreactive NK cells can reduce the incidence of GVHD and increase the survival rate.

    • >南京医科大学学报(社会科学版)
    • 医疗侵权诉讼举证责任倒置实施难点及对策

      2007(1):22-25. DOI: 10.7655

      摘要 (1512) HTML (36) PDF 0.00 Byte (99) 评论 (0) 收藏

      摘要:医疗侵权诉讼举证责任倒置实施的难点在于:医学技术的高度复杂性和医学发展的局限性、患者不配合检查诊治、医患关系脱离很久的病例、患方院外的情况等均可以造成医疗机构的举证不能。对策建议:建立完整的举证责任分配制度,规范使用自由裁量权,协调与《医疗事故处理条例》存在的冲突,制定一部专门处理医疗损害赔偿纠纷的法律,建立强制性与自治性相结合的复合性医事仲裁机制和医疗侵权赔偿的风险分散机制。

    • >南京医科大学学报(自然科学版)
    • 结核杆菌ESAT-6抗原及Flt3配体双表达核酸疫苗的构建与体外表达

      2007(1):23-26. DOI: 10.7655

      摘要 (1916) HTML (40) PDF 0.00 Byte (96) 评论 (0) 收藏

      摘要:目的:构建可同时表达flt3 配体(flt3-ligand,FL)和结核杆菌6kD早期分泌蛋白(ESAT-6)的重组pIRES质粒,并在大鼠肾小球系膜细胞(GMC)中表达,为进一步研究结核杆菌DNA疫苗提供实验基础。方法:采用PCR方法将FL和ESAT-6基因分别定向克隆入真核双表达载体pIRES。在酶切分析及序列测定后,用脂质体转染至GMC细胞,Western blot 鉴定其体外表达。结果:核酸序列测定证实重组质粒构建正确,该重组质粒在体外GMC细胞中能表达FL和ESAT-6两种蛋白。结论: 成功构建了结核杆菌FL和ESAT-6双顺反子真核表达质粒,并在体外实现了共表达。

    • >南京医科大学学报(英文版)
    • Analysis of results related to the percent free prostate specific antigen among men without prostate diseases in Xi’an area

      2007(1):25-28. DOI: 10.7655

      摘要 (1544) HTML (38) PDF 96.42 K (2060) 评论 (0) 收藏

      摘要:Objective: To measure the percent of free prostate specific antigen(fPSA) among men without prostate diseases in Xi’an area, and to study the relationship of percent fPSA with age and pathological grade, clinical stage of prostate cancer(PCa) with percent fPSA, and to analyze the difference between the data in China and the overseas data to determine appropriate reference range for Chinese male. Methods: A total of 713 participants were enrolled into the study, with PSA, fPSA in serum measured and the percent fPSA calculated. Out of 713 cases, 679 without prostate diseases were divided into 5 groups by age, and then the relationships of PSA, fPSA and percent fPSA with age were studied, respectively. The relationship of pathological grade and clinical stage with percent fPSA of the 34 participants with PCa was also studied. With the help of the related data of men without prostate disease, the appropriate reference range for Chinese male was established. Results: The increases in PSA or fPSA were correlated with age, while there was no significant correlation between age and percent fPSA. The percent fPSA was also correlated with pathological grade and clinical stage of PCa. The percent fPSA of men without prostate disease in Xi’an area was significantly lower than that in the related overseas data. The reference range of percent fPSA for Chinese male was≥15%. Conclusion: Percent fPSA might be more useful than PSA in the detection of prostate cancer. As the percent fPSA is decreased, the pathological grade is decreased, and the clinical stage is increased, the malignant degree is increased. The reference range of ≥15% is more appropriate for Chinese male.

    • >南京医科大学学报(社会科学版)
    • 医疗纠纷的可仲裁性研究

      2007(1):26-29. DOI: 10.7655

      摘要 (1653) HTML (52) PDF 0.00 Byte (81) 评论 (0) 收藏

      摘要:医疗纠纷的可仲裁性是建立医疗纠纷仲裁制度的前提要件,它决定以仲裁方式解决医疗纠纷的合法性。文章分析了医疗纠纷的法律属性和仲裁制度自身的发展,针对当前医疗纠纷可仲裁性所面临的问题,从仲裁立法与仲裁制度的设计两个方面提出自己的见解,以期为医疗纠纷仲裁制度的研究提供参考。

    • >南京医科大学学报(自然科学版)
    • 反相高效液相色谱法测定兔房水中万古霉素含量

      2007(1):27-30. DOI: 10.7655

      摘要 (2041) HTML (39) PDF 0.00 Byte (142) 评论 (0) 收藏

      摘要:目的:应用反相高效液相色谱(RP-HPLC)技术,建立定量检测兔房水万古霉素的方法。方法:房水样品中加入适量10%硫酸锌,高速离心,蛋白质沉淀后进行RP-HPLC检测。检测条件:分析柱为C8柱;流动相为甲醇(A)-醋酸(B),梯度洗脱。兔眼结膜囊内滴入纳米-万古霉素滴眼液后于120 min收集房水样本检测万古霉素浓度。结果:房水中药物峰与杂质峰分离良好,在0.5~20.0 μg/ml范围内,峰面积与浓度呈良好的线性关系。1、10、18 μg/ml 3种浓度的平均回收率为(98.670 ± 0.321)%,日内和日间RSD分别为(3.40 ± 1.30)%,(3.80 ± 2.20)%。兔眼房水中万古霉素药物浓度在滴入纳米-万古霉素滴眼液后120 min为:0.83 ± 0.05 μg/ml。结论: 建立了简便、敏感的检测房水中万古霉素的RP-HPLC方法,可用于眼内万古霉素药代动力学研究。

    • >南京医科大学学报(英文版)
    • Correlation between hair selenium concentration and gastric cancer

      2007(1):29-31. DOI: 10.7655

      摘要 (1592) HTML (44) PDF 81.86 K (2005) 评论 (0) 收藏

      摘要:Objective: To investigate the correlation between hair selenium(Se) level and gastric cancer. Methods: Atomic fluorescence spectrophotometer(AFS) was used to detect the Se level in hair. Results: The Se concentration in patients with gastric cancer ranged from 0.25 to 2.33 μg/g(0.825 ± 0.51 μg/g), and that of health individuals ranged from 4.23 to 9.21 μg/g(6.29 ± 1.68 μg/g). The results showed that the Se concentration in the patients’ hair was significantly lower than that in controls(P < 0.01). Conclusion: There is a correlation between hair concentration and gastric cancer.

    • >南京医科大学学报(社会科学版)
    • 产科领域医疗纠纷的成因和防范对策

      2007(1):30-33. DOI: 10.7655

      摘要 (1666) HTML (45) PDF 0.00 Byte (69) 评论 (0) 收藏

      摘要:文章分析了产科领域发生医疗纠纷的社会原因、经济原因和医源性因素。社会原因包括民众苛求医疗,对产科风险缺乏足够认识、医疗期望值过高,社会舆论导向错误和医疗举证责任倒置的影响;经济因素包括不同等级医院的价格竞争打烂了医疗秩序,医院间产生利益冲突,以及产妇及其家属企图以纠纷方式既要医院提供治疗,又不想支付医疗费用。纠纷发生的医源性因素包括产科医师存在自身技术缺陷,医务人员服务意识差,缺乏人文关怀,科室管理不规范,制度不落实,而科室间协作差,诊断意见不统一是重要的医源性因素。加强医院管理,规范医疗行为,提高医疗技

    • >南京医科大学学报(自然科学版)
    • 树突状细胞表面共刺激分子在哮喘小鼠中的作用研究

      2007(1):31-35. DOI: 10.7655

      摘要 (2091) HTML (42) PDF 0.00 Byte (105) 评论 (0) 收藏

      摘要:目的:研究哮喘小鼠骨髓来源树突状细胞(DCs)的表型及共刺激分子在哮喘发病中的影响。方法: 用卵清蛋白建立哮喘模型,利用rmGM-CSF和rmIL-4体外诱导骨髓细胞分化为DCs,流式细胞仪检测DCs表面共刺激分子的表达,混合淋巴细胞反应检测DCs刺激同种异体的T淋巴细胞增殖的能力。结果: 在两种细胞因子的作用下,从骨髓中可以诱导出大量的DCs;且均表达DCs的表面标志(33D1)。哮喘组DCs表达CD86分子的水平高于对照组,而CD40、CD80在两组之间没有差异;哮喘组与对照组DCs均能强烈刺激同种异体T淋巴细胞的增殖,哮喘组DCs的刺激能力明显强于对照组。结论: 哮喘组DCs的抗原递呈能力增强,表明DCs在哮喘的发病中可能起着重要作用。

    • >南京医科大学学报(英文版)
    • Detection of micrometastases in bone marrow and sentinel lymph nodes of breast cancer patients

      2007(1):32-35. DOI: 10.7655

      摘要 (1904) HTML (38) PDF 95.53 K (2185) 评论 (0) 收藏

      摘要:Objective: To study the sensitivity and clinical significance of HE-staining,IHC and RT-PCR in detecting breast cancer micrometastases in bone marrow and sentinel lymph nodes(SLNs). Methods:After general anesthesia, all patients underwent bone marrow puncture and sentinel lymph node biopsy(SLNB) by 1% isosulfan blue, and then HE-staining,IHC and RT-PCR were used to detect micrometastases. Results:Of 62 patients with breast cancer whose axillary lymph nodes showed negative HE-staining results, 15 cases presented with positive RT-PCR and 9 cases showed positive IHC results positive in bone marrow micrometastases detection. PT-PCR and IHC showed good uniformity(kappa=0.6945)and there was significant difference in detective rate between these two methods(χ2=4.1667,P = 0.0412). In SLN samples, 13 showed positive RT-PCR results, while 7 showed positive IHC results. PT-PCR and IHC showed good uniformity(kappa=0.6483)and significant difference was also found in detective rate between these two methods(χ2=4.1667,P = 0.0412). Both bone marrow and SLN samples were RT-PCR positive in 3 cases, which indicated that bone marrow micrometastases did not always accompany SLN micrometastases(χ2=0.067,P = 0.796). Conclusion: Even if no axillary lymph node involvement or distant metastases are present in routine preoperative examination, micrometastases can still be detected in bone marrow or SLNs. Because the bone marrow micrometastases and axillary node micrometastses are not present simultaneously, combination test of multiple indicators will detect micrometastases more accurately.

    • >南京医科大学学报(社会科学版)
    • “四诊合参”解决简易民事纠纷

      2007(1):34-37. DOI: 10.7655

      摘要 (1943) HTML (33) PDF 0.00 Byte (105) 评论 (0) 收藏

      摘要:将“四诊合参”的中医学思想运用到法庭调查、法庭辩论和法庭调解三个既紧密联系又相互独立的诉讼活动中,使之有机结合,能够真正实现简易程序的价值功能——诉讼效益与诉讼公正,并使“四诊合参”在法律的语境中得到新的诠释。

    • >南京医科大学学报(英文版)
    • Role of myeloid differentiation factor 88 in HSP60 signal transduction in dendritic cells

      2007(1):36-41. DOI: 10.7655

      摘要 (1883) HTML (34) PDF 1.07 M (2211) 评论 (0) 收藏

      摘要:Objective: To explore the role and mechanism of myeloid differentiation factor88(MyD88) in HSP60 signal transduction in dendritic cells. Methods:Mouse DCs were cultured from murine bone marrow cells. The DC marker CD11c was detected by flow cytometry, then DCs were divided into control group, HSP60 groupand RNA interference group. Control group was cultured under normal condition, and HSP60 group was cultured with 10 μg/ml of HSP60. RNA interference group was first cultured with MyD88 siRNA for12 hours and then HSP60 was added into the culture mixture. All groups were cultured for 48 hours. Immunochemistry was used to detect the concentration of MyD88 and NF- κB. Western blot was used to detect the concentration of MyD88. Flow cytometry and mixed lymphocyte reaction(MLR) were used to detect the phenotype and functional properties of DCs. ELISA was used to detect the concentration of TNF-α, IFN-γ and IL-12 in the supernatant. Results:The expression of CD11c in murine bone marrow DCs was 88.76%. HSP60 stimulation increased the expression of CD80, CD86, MHC-Ⅱin DCs and TNF-α, IFN-γ, IL-12 secretion in the supernatant. HSP60 stimulation also increased the level of MyD88 in the cytoplasm and promoted the shift of NF-κB to karyon and the proliferation of allogeneic T cells. MyD88 siRNA could decrease MyD88 and inhibit these effects induced by HSP60. Conclusion:HSP60 activates DCs through MyD88-dependent pathway. MyD88 plays a critical role in HSP60 signal transduction. Inhibition of MyD88 may be a novel way for treating disease correlated with HSP60.

    • >南京医科大学学报(自然科学版)
    • 经鼻持续气道正压通气对阻塞性睡眠呼吸暂停低通气患者血清脂联素和血压的影响

      2007(1):36-38. DOI: 10.7655

      摘要 (1975) HTML (38) PDF 0.00 Byte (113) 评论 (0) 收藏

      摘要:目的:探讨在阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者中持续气道正压通气(NCPAP)对血清脂联素和血压的影响,以及血清脂联素水平和高血压之间可能存在的相关关系。方法:选择23例男性中、重度OSAHS患者,于NCPAP治疗前1天和NCPAP治疗中、后检测血清脂联素、平均动脉压(MAP)。结果:OSAHS患者接受NCPAP治疗后,血清脂联素水平逐渐升高而MAP逐渐降低;血清脂联素水平和MAP显示与NCPAP治疗前相比,NCPAP治疗的第3天和第7天均未见统计学差异(均为 P > 0.05),但在NCPAP治疗的第14天差异显著(P < 0.01)。结论:OSAHS可引起低脂联素血症,后者可能参与了OSAHS并发高血压的发病,且可被NCPAP治疗所纠正。

    • >南京医科大学学报(社会科学版)
    • 动物致人损害的侵权责任及其赔偿探析

      2007(1):38-41. DOI: 10.7655

      摘要 (1891) HTML (38) PDF 0.00 Byte (108) 评论 (0) 收藏

      摘要:动物致害责任是一项古老的民事责任制度,历史悠久,但是我国学者对此研究甚少。近年来随着宠物豢养的增多,因动物致人损害而引发的民事纠纷不断出现,引起人们对这一特殊侵权行为的关注。文章着重研究了动物致人损害的侵权责任及其赔偿:动物致害责任作为一种古典的危险责任之一,采用无过错责任原则;动物致害责任中的动物是指为特定人所占有的,具有自主性伤害能力的动物,因此不包括处于野生状态的动物;且动物致害必须是动物之独立动作造成他人的人身、财产损害;动物致人损害的侵权责任,实行举证责任倒置原则;另外,动物致人损害侵权行为造成

    • >南京医科大学学报(自然科学版)
    • 持续气道正压通气对合并2型糖尿病的OSAHS患者胰岛素抵抗的影响

      2007(1):39-42. DOI: 10.7655

      摘要 (1985) HTML (35) PDF 0.00 Byte (107) 评论 (0) 收藏

      摘要:目的:通过观察了持续气道正压通气(CPAP)治疗对合并2型-糖尿病(2型-DM)的阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者的胰岛素抵抗(IR)的影响, 以验证OSAHS是否为引起糖尿病患者IR增加的独立危险因素。方法:选择25例合并2型-DM的肥胖OSAHS患者,将他们的呼吸暂停/低通气指数(AHI)为37.7 ± 18.0;体重指数(BMI)被控制在29.1 ± 2.3 kg/m2;通过6个月的CPAP治疗观察治疗前后反映IR的稳态模式评估(HOMA)指数变化。结果:在BMI无明显改变的情况下,6个月的CPAP治疗后HOMA指数较治疗前明显降低(1.84 ± 1.98 vs 3.33 ± 2.96, P < 0.0001)。结论:CPAP治疗可显著改善OSAHS患者的IR增高,提示OSAHS是IR的独立危险因素。

    • >南京医科大学学报(社会科学版)
    • 完善医疗事故赔偿的设想

      2007(1):42-44. DOI: 10.7655

      摘要 (1609) HTML (40) PDF 0.00 Byte (95) 评论 (0) 收藏

      摘要:通过对《医疗事故处理条例》中有关医疗事故赔偿的分析,指出其在保护患者及其家属合法权益的同时,还存在诸多不完善之处。根据现状,提出进一步完善医疗事故赔偿的几点设想,保护医患双方的合法权益。

    • >南京医科大学学报(英文版)
    • Ultrastructure of Amelanotic Melanocytes from Human Hair Follicles

      2007(1):42-46. DOI: 10.7655

      摘要 (2028) HTML (34) PDF 1.69 M (2330) 评论 (0) 收藏

      摘要:Objective: To investigate the ultra structure of amelanotic melanocytes (AMMC). Methods: The hair follicles obtained from normal human scalp by 0.50% collagenase type V treatment were washed with 0.1 mol/L phosphate buffer salt (PBS). Hair-follicle cell suspensions were prepared by trypsin treatment and cultured in melanocyte medium. Remaining keratinocytes were removed by differential trypsinization. 100μg/ml geneticin was used to eliminate the contaminating fibroblasts. At third passage, the cells were trypsinized, and then washed in phosphate-buffered saline and processed for transmission electron microscopy. Results: Under transmission electron microscope, the cultured cells showed round or oval shape, with single large nuclear and the karyotheca were double deck. There were obvious euchromosome within the nucleus, and sparse heterochromosome. There were various organelles in the cytoplasm, including plentiful melanosomes with nearly similar size, mitochondria, rough endoplasmic reticule(RER) and ribosome. The electron density granules in most of the melanosomes disposed along concentric circularities. Golgi apparatus in the cells was inconspicuous. Conclusion: The ultra structure of AMMC from human hair follicles is different from that of epidermal melanocytes, and these characteristics determine the functional immature of AMMC.

    • >南京医科大学学报(自然科学版)
    • 哮喘小鼠气道平滑肌细胞增殖及细胞周期蛋白D1表达的差异

      2007(1):43-46. DOI: 10.7655

      摘要 (2123) HTML (34) PDF 0.00 Byte (152) 评论 (0) 收藏

      摘要:目的:研究哮喘小鼠气道重建模型中气道平滑肌细胞(ASMC)的细胞增殖、细胞周期及细胞周期蛋白D1(cyclin D1)表达的变化,探讨cyclin D1对支气管哮喘气道重建中平滑肌细胞增殖过程及细胞周期的影响,进而为哮喘的诊断和治疗提供新的依据。方法:用Balb/c小鼠建立哮喘气道重建模型后进行ASMC原代培养,并以原代培养的正常小鼠ASMC为对照组,以四唑盐比色试验(MTT)检测细胞增殖能力,流式细胞术(FCM)测定细胞周期,FCM检测cyclin D1表达。结果:与对照组比较,哮喘组细胞的细胞增殖显著增快(P < 0.05);细胞周期中S 期比例明显增高;cyclin D1在胞质中表达明显增加。结论:在哮喘气道重建过程中ASMC经历了一定程度的过增殖过程,cyclin D1的表达增加参与了细胞增殖,气道重建环境可刺激气道平滑肌细胞增殖能力。

    • >南京医科大学学报(社会科学版)
    • 阿司匹林防治心血管疾病的认识过程与哲学思考

      2007(1):45-47. DOI: 10.7655

      摘要 (1712) HTML (38) PDF 0.00 Byte (88) 评论 (0) 收藏

      摘要:阿司匹林从发明至今已有百余年历史,随着对其研究的不断开展,其在心血管疾病方面的防治作用正广受人们关注。对阿司匹林步步深入的认识历史带给人们许多哲学思考:认识的反复与无限;认识的源泉与动力;理论与实践的相辅相成。

    • >南京医科大学学报(英文版)
    • Response of the xenograft endothelium in the concordant xenotransplantation

      2007(1):47-50. DOI: 10.7655

      摘要 (1683) HTML (37) PDF 1.17 M (1975) 评论 (0) 收藏

      摘要:Objective: To investigate the response of the xenograft endothelium in the concordant hamster to rat cardiac xenotransplantation and the mechanism of acute vascular rejection. Methods: The animals were divided into 5 groups randomly: control group, CsA group, splenectomy group, D0 splenectomy+CsA group and D3 splenectomy+CsA group. Hamster heart was heterotopicaly transplanted to rat abdominal cavity. The graft survival was monitored by palpation of the rat abdominal wall. The histological and ultrastructural changes of the xenogafts were investigated. NF-κB and P-selectin expression in the xenograft were detected. Heme Oxigenase-1 and Bcl-2 expression were also detected in the xenografts of different groups. Results: The mean survival time of the xenografts in control group, CsA group, splenectomy group, D0 splenectomy+CsA group and D3 splenectomy+CsA group was 3.4 ± 0.55, 3.8 ± 0.45, 6.4 ± 1.52, 30 and 7.4 ± 1.14 days. The rejected graft showed typical acute vascular rejection in control group, CsA group,splenectomy group and D3 splenectomy+CsA group. Endothelial cells of the rejected xenograft showed dramatic assembly of ribosomes and expansion of the rough endoplasmic reticulum. However, the endothelium of the long-term survived grafts in D0 splenectomy+CsA group showed normal architecture. NF-κB and P-selectin expression were detected in the rejected xenografts. HO-1 expression was observed in the long-term survived xenografts in D0 splenectomy+CsA group. Conclusion: The endothelial cells of the xenograft might be activated during the acute vascular rejection. Expression of HO-1 might inhibit the upregulation of NF-κB and adhesion molecular which decreases the activation of the endothelium of the graft.

    • >南京医科大学学报(自然科学版)
    • 氯胺酮雾化吸入对哮喘大鼠肺一氧化氮合酶表达的影响

      2007(1):47-50. DOI: 10.7655

      摘要 (2321) HTML (38) PDF 0.00 Byte (104) 评论 (0) 收藏

      摘要:目的:探讨氯胺酮雾化吸入对哮喘大鼠肺一氧化氮合酶(NOS)表达的影响。方法: 40只Brown Norway大鼠随机分为对照组(C组)、哮喘模型组(A组)、氯胺酮1组(K1组)、氯胺酮2组(K2组)、氯胺酮3组(K3组),每组8只。A组用卵蛋白辅以百日咳杆菌菌苗及氢氧化铝为佐剂注射致敏,2周后雾化吸入卵蛋白激发。K1、K2、K3组大鼠以同样方法致敏,在激发前分别雾化吸入12.5、25.0、50.0 mg/ml的氯胺酮。C组注射和雾化吸入PBS。取肺组织提取RNA,以RT-PCR法分析NOS mRNA的表达,并作肺组织病理学检查。结果:A组肺组织切片显示为急性气道炎症性改变,与A组相比,K1、K2、K3组炎症状态明显减轻。iNOS mRNA的表达与C组比较,A组明显增强,并有显著性差异(P < 0.01),与A组比较,K1、K2、K3组iNOS mRNA表达明显减弱,有显著性差异(K1组P < 0.05,K2组、K3组P < 0.01)。K1、K2、K3组间无明显差异。eNOS mRNA各组表达无显著性差异,nNOS在各组呈微弱表达。结论:氯胺酮雾化吸入可抑制卵蛋白所致的大鼠肺iNOS mRNA高表达,并改善肺部炎症,对肺损伤有保护作用。雾化吸入氯胺酮12.5 mg/ml已达到治疗效果。

    • >南京医科大学学报(社会科学版)
    • 以人为本——构建社会主义和谐社会的核心伦理和时代原则

      2007(1):48-52. DOI: 10.7655

      摘要 (1554) HTML (32) PDF 0.00 Byte (78) 评论 (0) 收藏

      摘要:和谐社会是一个不断实现的过程。和谐社会“重”在构建,但是在不同的历史时期,和谐社会的内涵、程度以及构建和谐社会的途径并不相同。“以人为本”这一原则既是人类力求解决人类和谐与冲突的张力失衡的核心伦理原则,也是我国社会的特质以及我国所处的特定的历史时空和方位所决定的时代原则。

    • >南京医科大学学报(英文版)
    • Qualitative and quantitative analysis of Chinese herb fructus chaenomelis

      2007(1):51-54. DOI: 10.7655

      摘要 (1936) HTML (41) PDF 323.51 K (2157) 评论 (0) 收藏

      摘要:Objective: To establish reliable methods for evaluating the quality of Chinese herb fructus chaenomelis. Methods: Qualitative analysis by Thin layer chromatography(TLC) , reference substances were Chaenomeles speciosa(Sweet) Nakai and oleanolic acid,a mixed solvent of chloroform-methanol(40 ∶ 1) was employed as the mobile phase,color developing agent was 10% sulfuric acid-ethanol solution.In the system of high performance liquid chromatography(HPLC), a Prontosil Eurobond C18 column (250 mm×4.0 mm,5 μm ) was used,the mobile phase was composed of acetonitrile-methanol-0.4% ammonium acetate solution(55 ∶ 25 ∶ 20),the flow rate was 1.0 ml/min with UV detected at 210 nm, the column temperature was maintained at room temperature. Results: In the system of TLC, oleanolic acid was separated successfully. In HPLC, the linear ranges of oleanolic acid and ursolic acid were 5.89~13.73 μg (R=0.9990)and 6.84~15.96 μg (R=0.9990), respectively. The average recoveries of oleanolic acid and ursolic acid were 97.52%(RSD=2 .58%), 98.21%(RSD=2.23%), respectively. Conclusion: The established TLC method can easily distinguish Chinese herb fructus chaenomelis from other commonly used crude drugs of the same family .The HPLC method for determining oleanolic acid and ursolic acid is simple, reproducible, accurate and feasible. The methods reported in this paper can be used scientifically and effectively to evaluate the quality of Chinese herb fructus chaenomelis.

    • >南京医科大学学报(自然科学版)
    • 爱维治对食管手术患者血糖、血浆胰岛素、乳酸与红细胞磷酸果糖激酶活性的影响

      2007(1):51-54. DOI: 10.7655

      摘要 (2073) HTML (42) PDF 0.00 Byte (83) 评论 (0) 收藏

      摘要:目的:观察麻醉前使用爱维治对食管手术患者血糖、血浆胰岛素、乳酸与红细胞糖酵解限速酶磷酸果糖激酶(PFK)活性的影响。方法:择期40例食管癌手术患者随机分为2组:对照组采用异氟醚吸入全麻,维持异氟醚呼气末浓度1.2 MAC;爱维治组麻醉方法同对照组,但麻醉前30 min予爱维治20 mg/kg静滴。分别于麻醉前(T1)、手术90 min(T2)、术后60 min(T3)、术后第1天晨(T4)和术后第2天晨(T5)抽取外周静脉血,测定血糖、胰岛素、血乳酸及红细胞内PFK活性,计算胰岛素敏感性指数(ISI)。结果:①两组血糖值与麻醉前值相比均升高(P < 0.05),T3时爱维治组血糖值低于对照组(P = 0.034); ②T4时爱维治组血浆胰岛素浓度与麻醉前值比较无显著上升(P = 0.732),但对照组血浆胰岛素浓度明显升高(P = 0.003),两组之间比较差异有显著性 (P = 0.035); ③两组ISI在T4时与麻醉前值相比均降低(P < 0.05),但爱维治组明显高于对照组(P = 0.045);④T4时爱维治组红细胞PFK活性与麻醉前值比较无明显降低 (P = 0.055),但对照组红细胞PFK活性明显降低(P = 0.001),两组之间比较差异有显著性(P = 0.044); ⑤T3时爱维治组血乳酸浓度未明显上升(P = 0.114),但对照组血乳酸浓度较麻醉前值明显升高(P = 0.000),两组之间比较差异有显著性(P = 0.036)。结论:爱维治能减轻食道癌手术患者手术应激引起的胰岛素抵抗和高血糖反应,增强红细胞磷酸果糖激酶的活性,抑制乳酸生成。

    • >南京医科大学学报(社会科学版)
    • 国内药品零售业发展中存在的问题与对策探讨

      2007(1):53-56. DOI: 10.7655

      摘要 (1853) HTML (37) PDF 0.00 Byte (80) 评论 (0) 收藏

      摘要:近年来国内药品零售业的发展一方面是行业规模持续快速扩张和企业的经营集中度进一步加深,另一方面市场竞争日趋激烈,差异化经营逐渐成为打造企业核心竞争力的取胜之道。发展中存在的问题突出体现在费率高企与亏损率不低、市场不成熟、竞争无秩序、药品流通环节多、批零一体化程度不高等方面。因此切实提高服务水平与服务质量,并进而在发挥企业内部优势和提高企业抗风险能力的基础上,避免各环节在相互争利中损失效益,便具有了十分重要的意义。

    • >南京医科大学学报(英文版)
    • Effect of IL-10 gene transmission on the PTg-stimulated splenocytes proliferation and Th1 cytokines production from experimental autoimminue thyroiditis rats

      2007(1):55-58. DOI: 10.7655

      摘要 (1825) HTML (38) PDF 834.38 K (2050) 评论 (0) 收藏

      摘要:Objective: To investigate the effects of gene therapy with IL-10 on PTg-induced proliferation of splenocytes and Th1 cytokine production from PTg-stimulated splenocytes. Methods: EAT rats were divided into four groups:group A (PBS+PLL) , group B(pORF+PLL), group C (pORFmIL10+PLL), and group D (pORFmIL10+ MEM). The substances mixed with lipofectamine were injected into the thyroid tissues of rats on the 18th dday after immunization. The rats were sacrificed at the 8th week. In vitro proliferative responses to ConA and different concentration of PTg were measured by culturing 4×105 splenocytes pulsed with 18.5KBq of[3H] thymidine for the final 12h and then harvested for liquid scintillation counting. In vitro splenocytes were cultured with PTg(25 mg/L). Th1 cytokine IFN-γ,TNF-αand IL-2 were detected by ELISA. Results: The proliferative response to PTg was suppressed in group C, compared with that of group A and B(P < 0.05). The levels of IFN-γ,TNF-αand IL-2 in the supernatant of PTg-stimulated splenocytes were 3548.25 ± 779.47 pg/ml, 27.66±10.50 pg/ml and 3617.73± 609.15 pg/ml, respectively, which were much lower in group C than those in group A and B(P < 0.01, P < 0.05, P < 0.001, respectively). Conclusion: IL-10 gene transmission in thyroid tissues could inhibit PTg specific proliferation of splenocytes from EAT rats and the secretion of Th1 cytokines from PTg-stimulated splenocytes.

    • >南京医科大学学报(自然科学版)
    • 2型糖尿病与恶性肿瘤临床相关性的研究

      2007(1):55-58. DOI: 10.7655

      摘要 (2210) HTML (35) PDF 0.00 Byte (116) 评论 (0) 收藏

      摘要:目的:从临床的角度探讨2型糖尿病与恶性肿瘤的相关性及临床意义。方法:调查了2000~2005年资料相对完整的所有含有2型糖尿病的死亡患者,据恶性肿瘤的存在与否分为肿瘤组(MG)和非肿瘤组(NMG),比较两组之间人数构成、性别、糖尿病病程、血压以及部分血生化学检查等指标,同时分析了肿瘤的发病率和分布情况。结果:2型糖尿病患者终末期平均年龄(73.57±10.17)岁,恶性肿瘤发生率高达28.35%(57/201);消化道肿瘤、血液系统肿瘤、肺癌占据前3位,收缩血压、HOMA-IR、血浆胰岛素、甘油三脂等水平升高。结论:2型糖尿病好伴发恶性肿瘤,且有其特殊的分布规律。恶性肿瘤确切的发病机制尚不明确,胰岛素抵抗可能是其原因之一。

    • >南京医科大学学报(社会科学版)
    • 浅谈公立医院医疗慈善救助基金的设立

      2007(1):57-59. DOI: 10.7655

      摘要 (1887) HTML (39) PDF 0.00 Byte (109) 评论 (0) 收藏

      摘要:在2006年全国卫生工作会议上首次明确提出公立医院要建立医疗救助基金。医疗慈善救助基金的建立有十分重要的现实意义,是解决目前公立医院欠费困扰、切实帮助弱势人群的需要;是顺应国际惯例,为慈善资金帮助医疗弱势人群搭建平台的需要;是解决民政部门实行的医疗救助基金医院无法受益的需要;也是树立公立医院良好的公益形象,为合法赞助提供渠道的需要。文章分析了基金建立的可行性和面临的主要困难,提出了基金的建立、管理、募集等一系列设想。

    • >南京医科大学学报(英文版)
    • Molecular mechanism of epididymal protease inhibitor modulating the liquafication of human semen

      2007(1):59-62. DOI: 10.7655

      摘要 (1828) HTML (41) PDF 370.24 K (2725) 评论 (0) 收藏

      摘要:Objective: To study the molecular mechanism of epididymal protease inhibitor(Eppin) modulating the liquafication of semen. Methods: Human semenogelin cDNA(nucleotides 82-849) and Eppin cDNA(nucleotides 70-423) were generated by PCR and cloned into pET-100D/TOPO.Recombinant Eppin and Sg were produced by BL21(DE3). The association of Eppin with Sg was studied by far-western and radioautography.In vitro the digestion of Sg by PSA in the presence or absence of recombinant Eppin was studied. The effect of anti-Q20E(N-terminal) and C-terminal of Eppin on Eppin-Sg binding was monitored. Results: Eppin binds Sg on the surface of human spermatozoa with C-terminal Eppin(aa75-133).Recombinant Sg was digested with PSA ,many low molecular weight fragments were produced, when Eppin is bound to Sg,then digested by PSA ,producing incomplete digestion and a 14.5-14.8 kDa fragmen. Antibody binding to the N-terminal of Eppin did not affect Sg digestion. Addition of antibodies to the C-terminal of Eppin inhibited the modulating effects of Eppin. Conclusion: Eppin modulates the digestion activity of PSA through binding Sg.The active site locates at C-terminal.

    • >南京医科大学学报(自然科学版)
    • 急性白血病血清可溶性细胞间黏附分子-1和血管细胞间黏附分子-1表达异常的初步研究

      2007(1):59-62. DOI: 10.7655

      摘要 (1928) HTML (40) PDF 0.00 Byte (75) 评论 (0) 收藏

      摘要:目的:观察急性白血病(AL) 血清中可溶性细胞间黏附分子-1(sICAM-1)和可溶性血管细胞间黏附分子-1(sVCAM-1)的表达水平及其临床意义。方法: 在60例初诊AL,以及治疗后达到完全缓解(CR)的16例患者中采集外周血分离出血清。采用酶联免疫吸附试验(ELISA)方法检测sICAM-1和sVCAM-1的水平,并探讨其与临床特征之间的关系。结果:在初诊AL患者中sICAM-1和sVCAM-1的水平明显高于CR组和正常对照组,CR组sICAM-1与sVCAM-1水平和正常对照组无显著差异(P < 0.01, P < 0.01)。在初诊AL中,能够达到CR的患者其sICAM-1与sVCAM-1水平要低于未达到CR患者(P < 0.05,P < 0.05)。在高白细胞白血病(WBC > 100 × 109/L)中,sICAM-1和sVCAM-1的水平和其他非高白细胞白血病无差异(P = 0.059,P = 0.146)。具有良好核型组和其对应组之间sICAM-1和sVCAM-1无统计学差异(P = 0.262,P = 0.558)。sICAM-1和sVCAM-1不同水平的患者生存期无差异。结论:AL血清sICAM-1和sVCAM-1表达水平异常且影响疗效,但无预后意义。

    • >南京医科大学学报(社会科学版)
    • 加强学生党建,建立保持学生党员先进性的长效机制

      2007(1):61-64. DOI: 10.7655

      摘要 (1697) HTML (32) PDF 0.00 Byte (83) 评论 (0) 收藏

      摘要:保持共产党员先进性要重视把好党员入党关。在重视大学生建党工作的同时,如何加强学生党建,提高党员质量是建立保持学生党员先进性长效机制的基础工程。文章立足于对南京医科大学学生党建工作的调研,结合学生党建工作实际,分析学生党建工作的基本情况和存在问题,对做好学生党建工作、确保高校学生党员先进性提出对策建议。

    • >南京医科大学学报(自然科学版)
    • 利福昔明胶囊治疗急性肠道感染随机双盲双模拟多中心疗效观察

      2007(1):63-65. DOI: 10.7655

      摘要 (2639) HTML (51) PDF 0.00 Byte (93) 评论 (0) 收藏

      摘要:目的:评价利福昔明治疗急性肠道感染的有效性和安全性。方法: 采用随机双盲双模拟试验方法,完成可供评估病例216例,其中试验组109例,对照组107例。试验组用药利福昔明胶囊(200 mg/次,每6 h 1次) + 氧氟沙星胶囊模拟胶囊(2粒,每日3次),对照组用药氧氟沙星胶囊(每次200 mg,每日3次) + 利福昔明胶囊模拟胶囊(2粒,每6 h 1次),疗程3天。结果:在治疗急性肠道感染中,利福昔明和氧氟沙星在止泻时间、大便常规复常率、细菌清除率、主要症状复常率等方面差异无显著性。两组的总有效率分别为95.41%和95.33%,不良反应发生率分别为3.64%、2.75%。利福昔明和氧氟沙星的疗效和安全性在统计学上差异无显著性。结论:利福昔明胶囊治疗急性肠道感染安全、有效。

    • >南京医科大学学报(社会科学版)
    • 培养创新型临床医学人才的思考

      2007(1):65-68. DOI: 10.7655

      摘要 (1811) HTML (38) PDF 0.00 Byte (99) 评论 (0) 收藏

      摘要:文章从创新及创新型人才的概念入手,通过调研指出,目前临床医学人才培养存在的主要问题,提出在新时期要转变教育教学观念、强化创新意识教育、增强创新思维训练、着力创新人格塑造,以培养创新型临床医学人才。

    • >南京医科大学学报(自然科学版)
    • 髂-股联合闭塞性疾病腔内治疗的技术探讨和疗效观察

      2007(1):66-69. DOI: 10.7655

      摘要 (1995) HTML (37) PDF 0.00 Byte (81) 评论 (0) 收藏

      摘要:目的:评价髂股动脉联合硬化狭窄,闭塞性疾病腔内治疗的安全性,临床成功率和短期通畅率。方法:2005年5~12月,19例髂股动脉联合病变的患者接受了腔内治疗,其中严重跛行(fontain Ⅱb)15例,静息痛3例,溃疡1例。髂动脉完全闭塞8例,严重狭窄(≥50%)11例。股总动脉严重狭窄或闭塞3例,股浅动脉狭窄6例闭塞13例。全部病例均采用对侧股动脉逆行入路跨过主动脉分叉对患侧髂股动脉行腔内治疗。结果:本组病例无围手术期死亡,总的并发症发生率5.2%(1例支架内血栓形成),技术成功率100%,平均随访5.5个月(1~8个月)短期随访腔内治疗通畅率为89%(17/19)。结论: 对于导致严重下肢缺血的髂股联合硬化闭塞性病变的腔内治疗是一安全、可靠易行的微创手段,具有良好的短期临床效果,但其中、远期疗效确有待进一步观察。

    • >南京医科大学学报(社会科学版)
    • 科学构建高校内部人员结构辩证关系的几点思考

      2007(1):69-71. DOI: 10.7655

      摘要 (1593) HTML (35) PDF 0.00 Byte (83) 评论 (0) 收藏

      摘要:如何在高等教育步入大众化教育阶段之际,不断深化教学改革、全面提高教育质量是中国高等学校面临的严峻挑战。文章通过分析高校内部人员结构的现状、特点,进而分析高等学校内部人员结构辩证关系的现状和存在的不足,提出通过转变思想、制定政策、科学实践三大途径,科学构建高校内部人员结构辩证关系,营造和谐互助的校园环境,促进教育教学工作,实现教育、学习、管理的协调发展。

    • >南京医科大学学报(自然科学版)
    • 乳腺癌细胞中p53、C-erbB-2、p21WAF1和 CDK4的表达及临床意义

      2007(1):70-73. DOI: 10.7655

      摘要 (2002) HTML (41) PDF 0.00 Byte (143) 评论 (0) 收藏

      摘要:目的:探讨乳腺癌组织中p53、C-erbB-2、p21WAF1和 CDK4的表达及临床意义。方法:采用免疫组化SP法检测120例乳腺癌组织石腊切片上p53、C-erbB-2、p21WAF1和 CDK4的表达。结果:p53、C-erbB-2、p21WAF1和 CDK4阳性表达率分别为53.3%、63.3%、58.3%、41.6%;阳性产物主要位于细胞核中,p53、C-erbB-2的表达与肿瘤组织分级(P < 0.05)、淋巴结转移(P < 0.01)显著相关,p21WAF1的表达与淋巴结转移(P < 0.01)显著相关,无淋巴结转移组明显高于有淋巴结转移组;与组织分级及PR、ER表达无显著相关。CDK4阳性表达与肿瘤组织分级(P < 0.05)、淋巴结转移(P < 0.01)显著相关,与PR、ER无相关。结论: p53、C-erbB-2、p21WAF1和 CDK4 与乳腺癌的发生发展密切相关,可作为判断肿瘤浸润转移、指导治疗和估计预后的参考指标。特别是p53的表达水平及淋巴结转移可能是判断乳腺癌的术后生存的独立有效指标,而综合应用上述指标可能更有助于预后的判断。

    • >南京医科大学学报(社会科学版)
    • 浅谈人本主义思想与图书馆管理

      2007(1):72-74. DOI: 10.7655

      摘要 (1577) HTML (40) PDF 0.00 Byte (135) 评论 (0) 收藏

      摘要:文章阐述了人本主义管理思想的基本观点,分析了在图书馆管理活动中如何以图书馆员为主体,实行人性化管理,以激发图书馆员的潜力,以利于更好地开展图书馆工作。

    • >南京医科大学学报(自然科学版)
    • 低声压动态超声造影技术对肝脏局灶性病变诊断价值的研究

      2007(1):74-77. DOI: 10.7655

      摘要 (2358) HTML (38) PDF 0.00 Byte (110) 评论 (0) 收藏

      摘要:目的:评价低声压超声造影技术在肝占位性病变中的鉴别诊断价值。方法:应用Sonovue 超声造影剂对181例共209个肝脏病灶行超声造影,并对造影结果进行分析。病灶经病理证实或随访6~12个月证实。结果:144个原发性肝细胞性肝癌(HCC)病灶和血管肉瘤、胆管细胞癌、结核各1个病灶呈现“快进快出”模式;25个血管瘤病灶呈现“慢进慢退”;21个肝硬化不典型增生结节,动脉期无增强,门脉期增强;5例转移性肝癌均为快速廓清,但动脉相增强方式不同;1例FNH和炎性假瘤呈“快进慢出”;5例脓肿为快速融合性增强;3例炎性假瘤2例结核未见造影增强。结论:低声压超声造影技术对肝脏局灶性病变定性诊断提供一种选择模式,超声造影较CDFI可显著提高局灶性肝脏病变的发现和定性诊断率。

    • >南京医科大学学报(社会科学版)
    • 以人为本,优化大学德育教育

      2007(1):75-77. DOI: 10.7655

      摘要 (1560) HTML (36) PDF 0.00 Byte (78) 评论 (0) 收藏

      摘要:2006年11月4日~11月24日,江苏省高校两课教师一行27人,赴加拿大多伦多大学考察大学教育,考察的着力点集中在学校管理、德育教育和心理咨询三方面。文章结合在多伦多大学的学习,集中探讨大学德育教育的理念和路径。

    • 八年制医学生临床和科研能力培养的思考

      2007(1):78-80. DOI: 10.7655

      摘要 (1762) HTML (36) PDF 0.00 Byte (77) 评论 (0) 收藏

      摘要:八年制医学教育是中国医学教育的新起点,现在正处于试行阶段。在2006年第三届中国八年制医学教育峰会上专家指出,八年制培养的学生应具有较强的临床实践技能和初步的科学研究能力,为发展潜力远大,创新能力和国际竞争力强的临床医学专门人才。笔者就八年制学生临床与科研能力的培养模式与培养目标进行了分析探讨,期望能对八年制医学教育学制改革提供一些有益的信息。

    • >南京医科大学学报(自然科学版)
    • 巨核细胞生长因子对实验性血小板减少小鼠的外周血小板的影响

      2007(1):78-80. DOI: 10.7655

      摘要 (1899) HTML (43) PDF 0.00 Byte (101) 评论 (0) 收藏

      摘要:目的:评估巨核细胞生长因子(MES)对实验性血小板减少小鼠的促血小板生成作用。方法:取雄性小鼠60只,按血小板数及体重均衡随机分6组。每组动物均腹腔注射给药连续8天,实验期间分别在0、5、9、14天测定各组动物的红细胞、血小板数目及血小板聚集实验,在15天测定网织血小板。结果:MES能使血循环中血小板数量升高,血小板聚集功能始终没有下降,网织血小板数升高。结论:MES这种巨核细胞生长因子能促血小板生成,且不引起血小板功能的变化。在血小板减少性疾病的治疗方面有着广阔的应用前景。

    • 112例淋巴结病变免疫球蛋白轻链限制性的检测

      2007(1):81-83. DOI: 10.7655

      摘要 (2417) HTML (39) PDF 0.00 Byte (87) 评论 (0) 收藏

      摘要:目的:比较原位杂交技术(In suit hybridization,ISH)和免疫组织化学方法(Immunohischemical,IHC)检测B细胞性淋巴瘤(B-cell lymphoma)和反应性增生病变(Reactive hyperplasia,RH)中免疫球蛋白轻链限制性的灵敏度和精确度。方法:收集112例淋巴结病变[B-cell lymphoma(n = 62)和RH(n = 50)],用ISH和IHC法检测免疫球蛋白轻链κ、λ的限制性。结果:ISH法,62例B细胞性淋巴瘤轻链有限制性45例(72.58%),50例淋巴结反应性增生病变没有限制性表达。IHC法,62例淋巴瘤有轻链限制性29例(46.77%),50例反应性增生病例6例有限制性表达(12%)。统计检验两种方法检测的结果显示表达有显著性差异(χ2 = 8.9338,P < 0.05)两种方法差异有显著性。结论:在诊断与鉴别诊断B细胞性淋巴瘤和反应性增生病变中,用ISH法检测轻链κ、λ mRNA 的基因序列,比用IHC法检测免疫球蛋白轻链蛋白κ、λ 限制性更灵敏和精确。

    • >南京医科大学学报(社会科学版)
    • 医学留学生教学改革的规则与展望

      2007(1):81-84. DOI: 10.7655

      摘要 (1576) HTML (39) PDF 0.00 Byte (74) 评论 (0) 收藏

      摘要:文章参照医学教育国际标准,结合南京医科大学全英文授课临床医学专业留学生教学工作的实践,对医学留学生培养方案、课程体系建设、师资培养、教学管理、留学生管理和国际交流合作方面进行了理论和实践研究,认为全英文授课有助于推动医学留学生教学工作的发展。

    • >南京医科大学学报(自然科学版)
    • 短串联重复序列检测法在江苏地区亲子鉴定的应用

      2007(1):84-86. DOI: 10.7655

      摘要 (2120) HTML (39) PDF 0.00 Byte (95) 评论 (0) 收藏

      摘要:目的:分析短串联重复序列(STR)基因座在江苏汉族人群亲子鉴定的应用。方法:取在本中心进行亲权鉴定的1 016个案例共2558份样本,提取DNA。用PCR特异性扩增出18个STR基因位点,聚丙烯酰胺凝胶电泳分型检测。以亲子关系指数(RCP)≥99.99%为认定亲权关系,以≥3个基因位点不符为排除亲权关系。结果:1016个案例中,认定亲权关系为758例,占75%,排除亲权关系为258例,占25%。19例表现出1个STR基因座突变现象。结论:STR基因检测位点多,多态性高、分布广泛、灵敏度高、易检测。联合使用多个STR位点进行检测可作为亲子鉴定的主要技术手段。

    • >南京医科大学学报(社会科学版)
    • 美国的标准化考试对完善中国考试制度的启示

      2007(1):85-88. DOI: 10.7655

      摘要 (1958) HTML (54) PDF 0.00 Byte (85) 评论 (0) 收藏

      摘要:标准化考试从一个侧面反映了美国教育的现状。保持世界一流教育的观念已深深扎根于美国人的潜意识之中。因此不断改进教育质量,提高教育水平,成为美国历届政府孜孜以求的目标,而标准化考试则被认为是达到这一目标的手段。在美国这个多元文化并存、强调个性自由发展的国度里,各界人士对标准化考试众说纷纭。同样,中国教育界对标准化考试也是各执一说。在这种情况下,标准化考试在美国的得失对中国完善考试制度提供了借鉴。

    • >南京医科大学学报(自然科学版)
    • 左心转流在心血管外科手术中的应用

      2007(1):87-88. DOI: 10.7655

      摘要 (2940) HTML (45) PDF 0.00 Byte (90) 评论 (0) 收藏

      摘要:目的: 探讨左心转流在心血管外科手术中应用的体会。方法:回顾性分析2001年1月至2005年12月应用左心转流治疗心血管外科病患者15例的经验。结果: 所有患者除1例术后出血经治疗后痊愈外,均取得了满意的治疗效果。结论:左心转流为心血管疾病的外科治疗提供了一个良好的方法。

    • 103例老年患者内侧半月板后角损伤的治疗

      2007(1):89-90. DOI: 10.7655

      摘要 (2625) HTML (105) PDF 0.00 Byte (76) 评论 (0) 收藏

      摘要:目的:探讨老年性内侧半月板后角损伤的临床特点及关节镜手术的方式。方法:60岁以上老年患者共103名,行关节镜检查术,术中证实内侧半月板后角损伤,并进行内侧半月板部分切除,回顾总结该组患者的临床表现、X线片和MRI的意义,并对患者的术前、术后进行Lysholmn评估。结果:老年患者内侧半月板后角损伤大多无明显的损伤史,但有临床症状,MRI和X线片敏感性不高,症状和体查是诊断的重要依据,术后患者症状明显好转。结论: 内侧半月板后角损伤是老年人的常见病,其诊断和治疗有其特殊性,关节镜下部分半月板切除能很好的改善老年人的膝关节的疼痛,提高生活质量。

    • 组合式外固定架结合有限内固定治疗SchatzkerⅥ型胫骨近端骨折

      2007(1):91-92. DOI: 10.7655

      摘要 (1930) HTML (38) PDF 0.00 Byte (97) 评论 (0) 收藏

      摘要:目的:探讨组合式外固定架结合有限内固定治疗SchatzkerⅥ型胫骨近端骨折的方法与疗效。方法:自2001年1月至2005年10月应用此技术治疗SchatzkerⅥ型胫骨近端骨折15例。技术要点包括:①闭合或小切口撬拨复位,大的主要骨折块做有限内固定;②以组合式外固定架完成骨折的外固定。其中6例跨膝关节固定。结果:全部病例随访6~20个月,平均14个月;外固定时间4~6个月,平均5.2个月; 14例一期愈合,1例开放骨折延期愈合,3个月后行二期自体髂骨植骨后愈合;4例开放骨折无深部感染发生,3例发生针道浅表感染,换药后感染控制。依据Sanders膝关节评分标准评定结果: 优5例,良6例,可3例,差1例。结论:组合式外固定架结合有限内固定是治疗SchatzkerⅥ型胫骨近端骨折较好的选择,它在恢复下肢轴线及关节稳定性方面具有一定的优势,并且可以明显减少切开复位的皮肤坏死和局部严重感染的并发症。

    • 固骼生在骨折与骨折不愈合治疗中的应用

      2007(1):95-96. DOI: 10.7655

      摘要 (2354) HTML (42) PDF 0.00 Byte (80) 评论 (0) 收藏

      摘要:

    • 急性胆囊炎经腹腔镜胆囊切除的可行性及相关处理

      2007(1):97-98. DOI: 10.7655

      摘要 (1736) HTML (43) PDF 0.00 Byte (76) 评论 (0) 收藏

      摘要:

    • 腹腔镜胆囊切除术中处理不同病理状况Calot三角无胆管损伤的体会

      2007(1):99-100. DOI: 10.7655

      摘要 (1867) HTML (38) PDF 0.00 Byte (80) 评论 (0) 收藏

      摘要:

    • 钬激光治疗尿道狭窄38例报告

      2007(1):100-101. DOI: 10.7655

      摘要 (1696) HTML (57) PDF 0.00 Byte (84) 评论 (0) 收藏

      摘要:

    • 高危前列腺增生症患者的TUVP治疗(附92例报告)

      2007(1):102-103. DOI: 10.7655

      摘要 (1754) HTML (42) PDF 0.00 Byte (86) 评论 (0) 收藏

      摘要:

    • 肠气囊肿症1例的CT诊断价值

      2007(1):104-105. DOI: 10.7655

      摘要 (1898) HTML (36) PDF 0.00 Byte (82) 评论 (0) 收藏

      摘要:

    • 高频彩超综合分析对乳腺癌腋窝淋巴结定性诊断的价值探讨

      2007(1):106-107. DOI: 10.7655

      摘要 (2092) HTML (38) PDF 0.00 Byte (76) 评论 (0) 收藏

      摘要:

    • 胎儿超声系统检查的探讨

      2007(1):107-109. DOI: 10.7655

      摘要 (1709) HTML (38) PDF 0.00 Byte (75) 评论 (0) 收藏

      摘要:

    • 不动杆菌属分离株的分布及耐药性分析

      2007(1):109-110. DOI: 10.7655

      摘要 (1728) HTML (41) PDF 0.00 Byte (81) 评论 (0) 收藏

      摘要:

    • PICC导管部分脱出原因分析与护理对策

      2007(1):111-112. DOI: 10.7655

      摘要 (2570) HTML (40) PDF 0.00 Byte (67) 评论 (0) 收藏

      摘要: