摘要:Objective:Carbamylated EPO(CEPO) is a derivative of erythropoietin(EPO) by subjecting it to carbamylation. It does not stimulate erythropoiesis, but effectively protects tissue from injury. The present study was to investigate the effect of CEPO treatment using in vitro models of hypoxia/reoxygenation(H/R). Methods:Cardiomyocytes were exposed to hypoxia(95% N2 and 5% CO2) for 1 hour followed by 4 hours of reoxygenation(95% O2 and 5% CO2). CEPO was administered after hypoxia, just before reoxygenation. The apoptotic cardiomyocytes were determined by flow cytometry. The level of protein was assessed by western blot analysis. Results: CEPO treatment significantly decreased the apoptotic cardiomyocytes by 54.20% compared with H/R group. Western blot analysis showed that CEPO administration increased the level of Bcl-2(an antiapoptotic protein) by 62.22% compared with H/R group. Conclusion: Acute administration of CEPO protected cardiomyocytes from H/R-induced apoptosis. CEPO protected cardiomyocytes with a concomitant upregulation of Bcl-2 after H/R injury.

摘要:Objective:ATP-binding cassette transporters(ABC) A1 and G1 play an important role in mediating cholesterol efflux and preventing macrophage foam cell formation. In this study, we examined the regulation of ABC transporters by high glucose in human vascular smooth muscle cells(VSMCs), the other precursor of foam cells. Methods:Incubation of human VSMCs with D-glucose(5 to 30 mM) for 1 to 7 days in the presence or absence of antioxidant and nuclear factor(NF)-κB inhibitors, the expressions of ABCA1 and ABCG1 were analyzed by real time PCR and Western blotting. Results:High glucose decreased ABCG1 mRNA and protein expression in cultured VSMCs, whereas the expression of ABCA1 was not significantly decreased. Down-regulation of ABCG1 mRNA expression by high glucose was abolished by antioxidant N-acetyl-L-cysteine(NAC) and NF-κB inhibitors, BAY 11-7085 and tosyl-phenylalanine chloromethyl-ketone(TPCK). Conclusion:High glucose suppresses the expression of ABCG1 in VSMCs, which is the possible mechanism of VSMC derived foam cell transformation.

摘要:Objective:To construct the adenoviral expression vector system containing human hepatocyte growth factor (hHGF) cDNA, and to further study the transduction efficiency and the expression of HGF in mesenchymal stem cells (MSCs). Methods:The HGF cDNA was amplificated from the expression plasmid pCMV-HGF, and was subcloned into the adenovirus shuttle plasmid pDC316-IRES-EGFP vector containing a green fluorescence protein (GFP) reporter gene. Virus Ad-HGF was produced by homologous recombination in HEK293 package cells. Bone marrow derived MSCs were harvested and cultured, and then were transduced with Ad-HGF. The efficiency of Ad-HGF transduction was assessed by FACS analysis using GFP gene expression. And HGF/MSCs were generated. The HGF concentrations in supernatants of HGF/MSCs were determined by ELISA using anti-human HGF monoclonal antibody. Results: The recombinant, named pDC316-HGF-IRES-eGFP, was digested with restriction enzyme, and the DNA sequencing of HGF was identical to the report in Genebank and did not reveal any mutation. GFP expression could be observed on the second day after packing of the linearized pAd-HGF in HEK293 cells and 7.15×1010pfu/ml titer of Ad-HGF was obtained. Forty-eight hours after transduction, 96.89% of HGF/MSCs were GFP positive. Peak concentration levels of hHGF(103ng/mL) in the cultured supernatants were detected on day 2 post-transduction, and the adenovirus-mediated expression of HGF by MSCs was maintained for at least 2 weeks in vivo. Conclusion:Our data demonstrated that the adenovirus expression vector system pDC316-HGF-IRES-EGFP has been constructed successfully, and their effective expressions also have been obtained in MSCs. This will provide material basis for the next study on liver regeneration after small-for-size liver transplantation.

摘要:Objective: To investigate the effects of synthetic retinoid CD437 and acitretin on cell proliferation, apoptosis, cycle arrest and Bax/Bcl-2 protein expression of melanoma A375 cell. Methods:MTT assay was used to determine the anti-proliferative effects of CD437 and acitretin on melanoma A375 cell. Flow cytometry was performed to investigate the influence of CD437 and acitretin on cell cycle and cell apoptosis. SABC immunocytochemistry was employed for detection of Bax/bcl-2 protein expressions. Results:10-5 mol/L CD437 was more effective than acitretin in inhibiting proliferation and inducing apoptosis of A375 cell after 24 h treatment, growth inhibiting ratio and apoptosis ratio(58.6%vs43.25% and 28.03%vs17.13%, P < 0.05 respectively). CD437 promoted G0/G1 arrest in melanoma A375 cell, however acitretin could not. CD437 and acitretin could up-regulate the expression of Bax protein and down-regulate the expression of bcl-2 protein(P < 0.05). Conclusion:CD437 is more effective than acitretin in inhibiting proliferation and inducing apoptosis and cycle arrest on A375 cell. CD437 may have more potentialities than acitretin for subsidiary treatment of melanoma. Mitochondrial apoptosis pathway is partially involved in two drugs inducing apoptosis on A375 cell.

摘要:Objective:To investigate the effect of nifedipine(calciumchannel blocker) on the expression of collagen in gingival fibroblasts invitro. Methods:Primarily gingival fibroblasts were cultured and incubated with various concentrations of nifedipine(108μg/L, 360μg/L and 1200μg/L)for 5 days. Gingival fibroblasts were primarily cultured derived from nifedipine responders and non-responders in the presence of 360μg/L nifedipine. Enzyme-linked immunosorbent assay was used to evaluate the amount of type I collagen. Cell proliferation was measured by cell counting with evaluating MTT value. Results:The expressions of collagen and cell proliferation were significantly different among the high concentration groups and the others on the fifth day, especially higher in 360μg/L and 1200μg/L groups and also different among nifedipine responders and non-responders. Conclusion:The expression of collagen and cell proliferation may be concerned with the biological mechanism for gingival overgrowth.

摘要:思考“看病难看病贵”的形成之因与缓解之策,是为了下更大的决心,逐步解决 “看病难看病贵”问题-形成 “看病难看病贵”既有医德失位-医技欠佳-医价偏高和医改滞后等直接原因,也有政府社会管理职能缺失的间接原因,还有制度与观念层面的深层次原因-因此,逐步解决“看病难看病贵”必须从医疗环境-政府职能和观念制度等方面实行系统治理,保障公民健康权,体现负担公平性,从根本上缓解看病难-看病贵-

摘要:Objective:To investigate the feasibility of the combination of detecting hypermethylated secreted frizzled-related protein 1(SFRP1) and secreted frizzled-related protein 2(SFRP2) in feces as a panel of biomarkers for colorectal cancer(CRC) screening. Methods:Methylation-specific PCR(MSP) was performed to analyze methylation status of SFRP1 and SFRP2 in a blinded fashion in tumor tissues and in matched stool samples from 39 patients with primary CRC, 34 patients with adenomas, 17 patients with hyperplastic polyps and 20 endoscopically normal subjects as normal controls. Simultaneously we analyzed the correlation of hypermethylated SFRP1 and SFRP2 with the clinicopathological features of CRC. Results:Hypermethylated SFRP1 was detected in 92.3%, 76.5%, 47.1% of tissue samples and in 89.7%, 64.7%, 35.3% of matched fecal samples from CRC, adenoma and hyperplastic polyp, respectively. Hypermethylated SFRP2 was detected in 87.2%, 67.6%, 35.3% of tissue samples and in 82.1%, 55.9%, 29.4% of matched fecal samples from CRC, adenoma and hyperplastic polyp, respectively. Of these two genes, at least one hypermethylated was 94.9%, 82.4%, 52.9% in tissue samples and 92.3%,73.5%, 47.1% in matched fecal samples from CRC, adenoma and hyperplastic polyp, respectively. In contrast, no hypermethylated SFRP1 and SFRP2 were detected in mucosa tissues of normal controls, only 2 cases of fecal samples was detected with hypermethylated SFRP1 and another 1 case was detected with hypermethylated SFRP2. Moreover, no significant associations were observed between hypermethylated SFRP1,SFRP2 and clinicopathological features of CRC. Conclusion:Hypermethylation of SFRP1 and SFRP2 in feces are novel epigenetic biomarkers of CRC and carried high potential for the remote detection of CRC as non-invasive screening method, and combined analysis of hypermethylated SFRP1 and SFRP2 in fecal could further increase the detection rate of CRC and premalignant lesions.

摘要:医疗损害赔偿无过错责任,可能导致“选择性医疗”“防御性医疗”的大面积采用-医疗费用的普遍上涨以及医务人员注意义务下降等诸多问题,而现行的法律制度可以对患者权益给予比较周到的保护,故不应建立医疗损害赔偿无过错责任制度-

摘要:This article provides the efficacy of the new device, Ligasure Vessel Sealing System(LVSS) for achieving hemostasis. LVSS is an alternative to suture ligatures, hemoclips, staplers, for ligating vessels and tissue bundles. This new device seals the vessels up to 7 mm in diameter by denaturing collagen and elastin within vessel wall and surrounding connective tissue.

摘要:知情同意权是患者在医疗活动中享有的一项法定权利-但基于医方在医疗活动中的多重角色和特殊地位,应该赋予其在一定条件下享有对应的抗辩权利-一般而言,在医方的强制医疗行为-履行告知义务的自由裁量行为-因不可抗力或者意外事件导致告知义务履行不能以及患者身处无力行使状态-拒绝和放弃-家属代理时具有过错等情况下,可以构成医方对患者知情同意侵权的抗辩事由-

摘要:Objective:Giant cell tumours(GCT) represent one of the most common benign tumours of bone. However, despite its benign nature they are aggressive lesions that have a tendency to recur. This study aims to report experience with the treatment of GCTs, and reviews the relationship between surgical management and clinical outcome. Methods:A retrospective review was performed with 70 patients (32 males and 38 females) who presented to our institution between 1991 and 2001 with GCT of bone. An evaluation of the oncological and functional results was conducted and patients were divided into three groups according to the treatment method; GroupⅠ:(46 patients) intralesional curettage and adjuvant therapy and packing with filling materials. GroupⅡ:(18 patients) en-bloc resection and arthrodesis or reconstruction. GroupⅢ:(6 patients) amputation. Results:The mean follow-up period was 10 years (range, 5-15 years). The overall rate of local recurrence was 14%, 22% in Group I, and only 4% in GroupⅡ and GroupⅢ. According to the Musculoskeletal Tumour Society(MSTS) score for functional outcome, the mean overall score for Group I was 27.9 (out of 30), 15.9 for GroupⅡ. Of note, the 9 patients within GroupⅡ who received endoprosthetic reconstruction, the mean overall MSTS functional score was 25.5. Conclusion:Intralesional curettage with adjuvant therapies and filling agents is often associated with a relatively high recurrence rate, however joint function is well preserved. Patients with more extensive, biologically aggressive, and/or recurrent tumours are best treated with en-bloc resection.

摘要:知情同意权是患者的一项基本权利,在“拒签事件”中受到理论挑战-文章分别从知情同意权利与医学行善原则的冲突-不同文化背景下知情同意权利实施的冲突和知情同意权在民法学上程序正义与实体正义的冲突进行分析,并在法伦理学的视角下对知情同意原则的适用提出反思-

摘要:Objective:To investigate the expression of cyclooxygenase-2 and its pathological effect in the experimental nonalcoholic fatty liver of rats, and to explore its possible mechanism. Methods:The rat NAFLD model was established by giving a fat-enriched diet. The blood samples were obtained form abdominal aorta and the levels of serum ALT, AST and IL-1, changes in the hepatic tissue 6-k-PGF1α TXB2 were measured. The expression level of COX-2 in rats livers were assayed by immunohistochemistry, RT-PCR and Western-blot. Results: Light microscope analysis revealed that hepatocytes were injured in the model group and slightly in the treatment group. The levels of serum TXB2 and IL-1 in the fatty liver rats were increased. Compared with the model group, the IL-1 and TXB2 increased significantly(P < 0.05), on the contrary, compared with the normal group, the hepatic tissue 6-Keto-prostagland decreased significantly in the model group(P < 0.05), the treatment group also increased but P > 0.05. There was no positive expression of COX-2 in hepatic tissue of normal rats. In the model group, there was positive expression of COX-2 antigen and the number of COX positive cells progressively increased at 4, 8, 12 wks. The intensity of expression of COX-2 had significantly increased(P < 0.05） and the intensity of COX-2 expression in the treated group decreased remarkably compared with the model group(P < 0.05). The expression of COX-2 mRNA and the level of COX-2 protein were significantly stronger in the liver of model rats compared with normal rats, and significantly weaker in treated rats, than in 8W and 12W model rats(P < 0.05). Conclusion:The increase of COX-2 expression in NAFLD is closely associated with the severity of liver inflammation and damage. COX-2 may play an important role in the progression of rat NAFLD, and the expression of COX-2 mRNA is downregulated by cyclooxygenase-2 inhibitor, which can depress the oxidative stress and control inflammatory response efficiently.

摘要:2003年8月27日审议通过的《中华人民共和国行政许可法》实施数年来,取得了巨大的成就,但也存在着一些问题-文章从立法宗旨-立法原则-主要制度等方面对行政许可法进行了阐述,指出了其在实施中存在的问题,并对此进行了多方面的分析,最后提出了将行政许可法落实到位的具体措施-

摘要:Objective:To investigate clinical features and imageology of renal cysts of high density, containing proteinaceous fluid and increase the diagnosis and treatment level of this special type renal cyst. Methods:Six cases were proven to be renal cysts of high density(pathologically) from 2002 to 2007 were reviewed. Among 6 cases, 1 was in the upper pole of kidney, 4 were medial and 1 was located in the anus perineum . All were 2-5 cm in size. Ultrasonography(US) excretory unognaphy, multiphase CT and renal angiography DSA imaging was performed for preoperative diagnosis. The preoperative diagnosis found renal neoplasms in 4 and renal cysts in 2. All of them were operated by partial nephrectomy. Results:All of the 6 renal high density renal masses were resected surgically, which were proved pathologically to be renal cysts; high density present. All of them contained proteinaceous fluid with benign cyst walls on histologic examination. No recurrence was seen in any of these cases during a long follow-up. Conclusion:CT and B-US have a higher diagnostic value, which can show the internal shape and character better. B-US or CT guided puncturing biopsy can be better applied to atypical renal cysts. Once the correct diagnosis is acquired, laparoscopic surgical treatment should be carried out.

摘要:在长期的中国革命与建设中,毛泽东同志致力于马克思主义中国化,形成了独特的思想-总体来看,毛泽东同志关于马克思主义中国化的思想,主要包括了相互衔接的几个方面:学习马克思主义,抓好马克思主义中国化的前提;实施调查研究,奠定马克思主义中国化的基础;注重理论与实践相结合,把握马克思主义中国化的关键;强调实事求是,坚持马克思主义中国化的方向;推进理论创新,加快马克思主义中国化的进程-

摘要:Objective:To investigate the expression of autophagy related gene; Beclin1 in human non-small cell lung cancer(NSCLC) tissues. Methods:Protein expression of Beclin1 was determined by immunofluorescence staining and Western Blot, mRNA expression was analyzed by RT-PCR. Results:Immunofluorescence staining revealed that the level of Beclin1 expression in lung cancer was significantly lower than that in adjacent noncancerous tissues and normal tissues(expression rate 8.3%, P=0.000). The Beclin1 mRNA expression in lung cancer, adjacent noncancerous tissues and normal tissues was 1.372 (±0.475) 1.721 (±0.521) and 1.553(±0.554) when F = 15.0, P < 0.01. Beclin1 protein expression in lung cancer, adjacent noncancerous tissues and normal tissues was 3.453(±0.852) 5.423(±1.351) and 6.878(±0.997) F = 11.2, P < 0.01. Conclusion:The protein and mRNA expression of Beclin1 in lung cancer was much lower than those in and around cancer tissues and normal tissues, those differences having statistical significance. However in adjacent noncancerous tissues and normal tissues, the expression showed no difference.

摘要:学习型组织理论是目前西方管理界较为推崇并正在进行研究和实践的一种前沿的管理理论,本文从学习型组织的起源-内涵及学习型组织理论与思想政治工作理论的关系入手,对思想政治工作如何借鉴学习型组织理论,并结合自身特点有所创新作出了探讨-

摘要:健康是人类生存和发展的基础,也是人类获得幸福的基本前提-从现代文明发展的道路来看,生产发展-生活富裕-生态良好-生命健康-和谐幸福无疑是和谐社会的应有之意和内在要求,而人类健康作为判断人与自然以及人与社会之间关系和谐与否的标尺建设,在构建和谐社会的过程中发挥着非常重要的作用-文章将健康模式和医疗模式进行了对比,从健康管理的视角,着重探讨了推进健康保障是比推进医疗保障更经济-更有效-更高价值的社会保障模式-

摘要:Objective:Several studies have analyzed the clinical profiles of patients diagnosed with hypertrophic cardiomyopathy(HCM). We sought to identify its characteristics in a regional cohort of Nanjing and its adjacent region. Methods:Clinical profiles of 121 referred patients were collected and analyzed retrospectively. Data including family history, clinical symptoms, electrocardiography and recent echocardiography were collected. Results: The mean age of this population was 42±17 years(range from 6 to 76) at diagnosis of HCM. Most patients were male(60%). 48 patients(39.7%) has a family history, 19 had a sudden death in a first degree relative and 96(79.3%) were recognized with cardiac symptoms. Left ventricular outflow obstruction(gradient≥30 mmHg at rest) was presented in 26(21.5%) patients. ECG abnormalities comprised of arrhythmia in 54(51.4%) and abnormal T wave in 72(68.6%) patients. FS were higher in female than male(P = 0.001). Among younger patients(age≤50 years), LVDd and LVWP were smaller in females than males(P = 0.042 & 0.023 respectively). In older patients(age＞50 years), LVDs was higher in male(P = 0.016) and EF was higher in female(P = 0.048). Conclusion:HCM patients in the region are almost diagnosed with the presentation of cardiac symptoms; those without any symptoms could be recognized by ECG and family screening. Most cardiac hypertrophy affects the interventricular septum. LVDd, LVWP, LVDs, FS and EF showed significant differences related to age and gender.

摘要:在整个医疗保险体系中,作为医疗保险费用管理和控制的关键环节,如何选择适当的医疗费用支付方式是一个重要内容-为兼顾效率与公平这一双重目标,作者提出:一是要以总额预算付费方式作为医保费用支付体系的基础;二是要实行多元化-混合式的费用支付方式;三是结合中国国情有选择性的-分阶段的实施按病种付费方式-

摘要:Patients with abrupt onset of chest pain, ischemic ECG abnormalities and elevated levels of cardiac markers could be given a diagnosis of acute myocardial infarction. However, some other diseases should be taken into consideration in this clinical setting when coronary arteries are proven to be normal. Here we report a case of acute myopericarditis with clinical presentation of myocardial infarction and normal coronary anatomy. The Herpes Simplex Virus II was considered as the organism causing myopericarditis and the patient was recovered by the treatment with valacicloavir. A precise diagnosis is a prerequisite of successful treatment and favorable prognosis.

摘要:住院医师培训是医学发展和实施人才战略的重要环节-本文总结了青岛市立医院住院医师规范化培训的实践,提出了培训思路与改进建议,为提高住院医师规范化培训水平作出了有益的探索-

摘要:住院医师培养已从单纯学历提高的传统模式中转变出来,能力的培养特别是综合能力的培养已成为主流-本文阐述了住院医师能力构成,强调加强医学专业技能培养的同时,突出住院医师人文知识的培养-倡导养成终身学习的习惯-注重人文知识学习构筑交流平台,强化住院医师的团队合作精神为有效途径,培养和提高住院医师处理突发公共卫生事件和综合能力为实现手段-

摘要:在激烈的医疗市场竞争中,如何求生存求发展已成为医院经营中最大最深刻的课题-文章从门诊导医护理工作出发,提出树立“顾客满意”(CS)经营理念,强化服务质量意识,规范服务,改进工作质量,是实现患者及社会各方满意,提高医院竞争力的有力措施-

摘要:目的:分离和鉴定人类IL-10-IL-10受体(IL-10Rα)-IL-4及其受体IL-4Rα启动子序列,并分析IL-10-IL-4以及其受体的启动子在人类单纯疱疹病毒1型(HSV-1)激活卡波济肉瘤相关疱疹病毒(KSHV)过程中的启动活性-方法:以人类基因组DNA为模板,PCR扩增IL-10-IL-10Rα-IL-4Rα启动子区序列,并分别克隆载入pGL-3基本载体中虫荧光素酶(Luciferase)报告基因的上游-进一步将上述构建的重组质粒与本室保存的含IL-4启动子重组质粒分别转染HSV-1感染的BCBL-1细胞,并作Luciferase活性检测-结果:分离了IL-10-IL-10Rα和IL-4Rα的启动子区域序列,并成功克隆了含启动子序列重组报告质粒;HSV-1感染的BCBL-1细胞在进一步转染了IL-10-IL-10Rα-IL-4和IL-4Rα启动子重组报告质粒后,其Luciferase值与相应的对照比较显著升高(P < 0.05)-结论:在BCBL-1细胞中,HSV-1可通过直接激活IL-10-IL-4以及相应受体的启动子来上调它们的表达;在HSV-1感染的BCBL-1细胞中,IL-10和IL-4可能对KSHV的裂解复制起到促进作用-

摘要:当前,普通医科院校学报在缺乏顶尖水平稿件的情况下,编辑专业修养的高低对刊物的学术影响力有重要作用-本文结合国外优秀期刊的经验,及国内多数医科院校学报的实际工作情况,对医科院校学报及其编辑专业修养上存在的问题进行了初步的探讨,提出做学者型编辑以促进医学学报的发展-

摘要:目的:研究眼镜蛇毒因子(CVF)耗竭体内补体对Thy-1肾炎大鼠肾小球系膜细胞(GMCs)凋亡病变及其生长阻滞和DNA损伤诱生蛋白45α(Gadd45α)基因表达的影响-方法:利用抗胸腺细胞抗血清(ATS)复制大鼠Thy-1肾炎(Thy-1 N)模型-大鼠随机分为正常对照组-Thy-1 N组和CVF+Thy-1 N组-取各组大鼠血清检测补体CH50活性-另取各组大鼠肾皮质,行电镜和TUNEL染色检查判断其肾组织细胞凋亡的病理变化;用RT-PCR和real-time PCR检测大鼠肾组织凋亡相关基因,即Gadd45α mRNA丰度;用Western blotting检测Gadd45α蛋白表达水平-结果:CVF+Thy-1 N组补体CH50活性明显下降,与Thy-1 N组和正常对照组相比差异明显(P < 0.01)-电镜和TUNEL染色观察到Thy-1 N组大鼠GMCs有凋亡病变,而CVF+Thy-1 N组凋亡病变则显著减轻-Thy-1 N组大鼠肾皮质Gadd45α mRNA 40 min开始上调,3 h达到峰值,而用CVF处理的Thy-1 N大鼠,Gadd45α mRNA丰度则明显低于Thy-1 N组(P < 0.01);Thy-1 N组Gadd45α蛋白水平3 h时显著增多,而CVF+Thy-1 N Gadd45α蛋白水平明显低于Thy-1 N组(P < 0.01)-结论:CVF耗竭补体可显著减轻大鼠Thy-1肾炎凋亡病变并抑制Gadd45α基因的表达-

摘要:随着世界经济全球化进程的加快,信息技术的广泛运用和不断更新,重视信息技术的发展已成为酒店业的共识-在这全新的时代背景下,高校酒店业如何在激烈的市场竞争中充分利用资源,克服自身的不足,是值得高校管理者思考的问题之一-作者分析当前高校酒店业信息化方面存在的差距以及未来的发展趋势,并为发展高校酒店的竟争力提出一系列建议-

摘要:加强大学生心理健康教育工作是促进大学生全面发展的重要途径和手段,是高校德育工作的重要组成部分-辅导员应正视心理育人的重要性,在平日的工作中将思想育人与心理育人有效结合,积极开展工作,培养出具有健全人格,健康体魄,能够全面发展的高素质人才-

摘要:目的:探讨CD137信号对CIK细胞的增殖和功能调节作用-方法:分离健康志愿者外周血单个核细胞(PBMCs),实验组在常规CIK培养体系中加入CD137单抗(CD137-CIK组),对照组在常规CIK培养体系中加入鼠IgG1同型对照(IgG1-CIK组)-苔盼蓝拒染法细胞计数分析细胞增殖;流式细胞术检测细胞表型及细胞内因子的表达;LDH酶释放法检测CIK细胞杀伤活性-结果:CD137-CIK组细胞体外扩增效率显著高于IgG1-CIK组,CD137-CIK组细胞浓度最高达(9.87 ± 0.57)×106/ml;IgG1-CIK组最高为(7.02 ± 0.68)×106/ml;CD137-CIK组和IgG1-CIK组细胞中CD3+CD56+细胞比例至28天分别达到(39.86 ± 4.69)%和(29.14 ± 5.12)%(P < 0.05);经CD137mAb作用后,其体外杀伤肺癌细胞株A549活性明显高于对照组(P < 0.05);第0-7-14-21天流式检测IFN-γ表达,实验组CD3+CD56+细胞IFN-γ的表达显著高于对照组-结论:CD137mAb介导的共刺激信号可以促进CIK细胞的体外增殖活性,并增强CIK细胞体外抗瘤作用-其中CD137-CIK组CD3+CD56+细胞的比例及其IFN-γ表达显著提高,这可能是CD137信号增强CIK抗瘤作用的重要原因-

摘要:随着知识经济时代高等教育社会地位的不断提高,发达国家如英国正在大力改革研究生教育以增强自己的竞争优势,其研究生教育改革和发展体现出众多特点,学位体系开放多样化-招生范围扩大化-国际化,课程内容综合化以及质量管理标准化的趋势越来越明显-尽管我国与英国研究生教育有很大差别,但许多改革可以借鉴-

摘要:目的:探讨哮喘大鼠肺组织中血管紧张素Ⅱ(AngⅡ)两种受体亚型(AT1R-AT2R)和β2肾上腺素受体(β2-AR)的表达及缬沙坦对两类受体的影响-方法:将50只SPF级Wistar大鼠随机分为正常对照组(A组)-哮喘组(B组)-缬沙坦15 mg/kg组(C1组)-缬沙坦30 mg/kg组(C2组)-缬沙坦50 mg/kg组(C3组)-用10%鸡卵白蛋白(OVA)致敏和1%OVA激发大鼠建立哮喘模型;分别用逆转录-聚合酶链反应(RT-PCR)和Western blot法检测肺组织中ATR和β2-AR mRNA和蛋白表达-结果:与A组相比,B组的AT1R阳性表达率明显增高(P < 0.05)-缬沙坦干预后,C1-C2-C3组的AT1R阳性表达率均明显下降(P均 < 0.05);相关性分析提示AT1R的表达与缬沙坦剂量呈负相关(r = -0.96)-A组未见AT2R表达,B组AT2R表达明显增加-缬沙坦干预后,C1组无AT2R表达,C2-C3组AT2R表达量低于B组(P均 < 0.01)-与A组相比,B组的β2-AR阳性表达率明显下调(P < 0.01),缬沙坦干预后,C1-C2-C3 组的β2-AR阳性表达率均明显上调(P均 < 0.05)-结论:大鼠肺组织中存在血管紧张素Ⅱ受体,并在哮喘发作时被活化,可能参与哮喘发病的病理生理过程-ATR与β2-AR的变化可能存在相关性-哮喘大鼠肺组织中AT1R和AT2R表达增加,β2-AR表达下调-AT1R拮抗剂缬沙坦能抑制AT1R表达,上调β2-AR表达-

摘要:目前在高校附属医院建设中,教育教学职能的优化是重要问题,本文提出了优化非直属附属医院教育教学职能的价值,并根据存在的问题和挑战,提出要强化学校对优化附属医院教育教学职能的指导,加强医院领导认识的重要性并结合医院自身的有关条件,不断优化附属医院教育教学职能等六项措施-

摘要:目的:探讨表皮生长因子(EGF)对人激素非依赖性前列腺癌PC-3细胞环氧化酶-2(COX-2)表达的影响及可能的机制-方法:以1-10-100 μg/L不同浓度的EGF作用于PC-3细胞24 h,采用逆转录聚合酶链反应(RT-PCR)和Western blot测定COX-2的表达变化;再将磷脂酰肌醇-3激酶阻断剂(LY294002,20 μmol/L) -促分裂原活化蛋白激酶阻断剂(SC203580,20 μmol/L)和表皮生长因子受体阻断剂(ZD1839,2 μmol/L)分别与10 μg/L EGF作用于PC-3细胞,Western blot测定C0X-2蛋白的变化-结果:PC-3细胞在1~100 μg/L浓度的EGF刺激以后的表达显著上调,和对照组有明显区别(P < 0.05)-SC203580-LY294002和ZD1839显著抑制COX-2上调(P < 0.05)-结论:EGF可诱导PC-3细胞COX-2的表达上调,促分裂原活化蛋白激酶和磷脂酰肌醇-3激酶参与了其中的调节过程-

摘要:本文根据教学论的方法和临床教学医院的实际情况,建立了一套对临床教学见习质量进行控制和综合评价的体系,采用Delphi法针对教学医院临床教学评价的多方面的指标进行多因子的筛选以及各因子之间的关系的描述和计算,采用图论中有向树的概念建立了数学模型,力图使计算简单且能得到较为完整-合理的评价结果-

摘要:目的:探讨中药白芍的有效成分——芍药苷对人胃癌SGC-7901细胞株NF-κB的活性以及细胞凋亡的影响-方法:将芍药苷以不同浓度及不同时间作用于胃癌SGC-7901细胞-应用Western Blot方法检测各组胞核NF-κB表达情况,并应用胞核NF-κB活性检测(ELISA法)进一步确定胞核内NF-κB的表达情况,流式细胞仪检测联合应用芍药苷及5-氟尿嘧啶(5-FU)对胃癌细胞凋亡的影响-结果:芍药苷对胃癌SGC-7901细胞胞核NF-κB表达有明确的抑制作用,Western Blot以及胞核NF-κB活性检测结果均显示这种抑制存在着时间及剂量依赖现象,流式细胞术检测结果表明芍药苷对5-FU诱导的肿瘤细胞凋亡有明显的促进作用-结论:芍药苷抑制了细胞内NF-κB的表达,并借助这种作用增强5-FU诱导的细胞凋亡效应-

摘要:从医学形态学素材资源库实例出发,探讨了现代学习理论中行为主义学习理论-人本主义学习理论和建构主义学习理论对于医学形态学素材资源库素材选取的指导意义-

摘要:目的:利用已构建好的在肝癌细胞中定向表达p16基因的腺病毒载体AdAFP-p16,研究腺病毒介导p16基因的表达对肝癌裸鼠移植瘤的抗癌活性-方法:培养肝癌细胞SMMC-7721,于裸鼠右侧腹部近腋下皮肤注射1×106细胞数细胞悬液,建立裸鼠肝癌移植瘤模型-成瘤后给于瘤体内AdAFP-p16病毒注射治疗,病毒总量1×109 pfu/只,观察AdAFP-p16对肝癌模型的抗肿瘤疗效以及p16基因表达引起的肝癌细胞病理变化-结果:AdAFP-p16能够介导p16基因在肝癌细胞中特异性表达,具有明显的肿瘤生长抑制作用,抑制率可达60.12%(P < 0.01),引起肝癌细胞的坏死-结论:AFP启动子控制的p16基因在肝癌细胞中定向表达,能够抑制肝癌模型的生长,对肝癌综合治疗具有重要意义-

摘要:通过对6名大学英语教师的课堂观察,结合相关二语阅读的教学研究文献,调查并分析了国内某高校阅读教学的基本模式及其潜在不足-同时对一个英国二语课堂的系列观察显示阅读教学存在有别国内课堂的其他做法,尽管教学背景不同,这些做法一定程度上能够给中国的外语教师提供有益的启示,主要表现在三方面:①注重阅读过程;②改变教师角色;③适当改编现有教材-

摘要:目的:通过比较2 Hz及100 Hz跨皮神经电刺激(TENS)治疗大鼠慢性单发佐剂性关节炎症痛时三种神经肽含量的变化,探讨其在TENS治疗中的作用-方法:采用油包水型完全弗氏佐剂(CFA)右侧踝关节腔内注射致炎,TENS治疗,放射免疫分析法测定致炎前及致炎后1~9周大鼠脊髓背半侧组织中与疼痛有关的神经肽:八肽胆囊收缩素(CCK-8)-孤啡肽(OFQ)及内吗啡肽-2(EM2)含量的动态变化-结果:致炎后2~5周,CCK-8含量明显升高(P < 0.05,P < 0.01),100 Hz TENS使关节炎时脊髓CCK-8含量升高的时间推迟至第3周且维持时间明显缩短;100 Hz较2 Hz TENS能更明显的升高炎症后期降低的脊髓OFQ含量;致炎后1~9周,关节炎大鼠脊髓EM2含量均明显下降(P < 0.01),100 Hz TENS 使炎症所致EM2降低时间推迟至第2周,2 Hz和100 Hz TENS均可使炎症后期(5~9周)EM2含量恢复正常-结论:2 Hz及100 Hz TENS对慢性炎症痛大鼠脊髓组织中有关的神经肽,CCK-8-OFQ和 EM2的影响可能是两者疗效存在差异的机制之一-

摘要:兴趣是学习的强大动力,在医学临床实习的教学中,注重教者魅力-引入情景设置和病案导入等兴趣诱导方法,可激发学生的学习兴趣和探索欲望,培养临床诊疗思路,增强教学效果-

摘要:《牙齿美容修复》是一门为了适应新医学模式下的人才培养需要而开设的口腔医学选修课,主要面向非口腔专业医学生-通过问卷调查的方式对《牙齿美容修复》选修课的必要性-课程设置以及课程效果等进行总结-分析-结果表明《牙齿美容修复》经过两年的教学实践,96%的学生对牙齿美容修复选修课持欢迎态度,98.4%的学生认为达到或者基本达到了预期的学习目的,取得了预期的教学效果-

摘要:目的:研究过氧化物酶增殖因子激活受体γ(PPARγ)途径在选择性COX-2抑制剂尼美舒利影响胃癌细胞凋亡和增殖机制中的作用-方法:体外培养人胃癌SGC-7901细胞,给予不同浓度PPARγ抑制剂GW9662及尼美舒利干预,同时设立对照组-采用四甲基偶氮唑蓝(MTT)比色法检测药物作用后细胞生长抑制率,流式细胞仪(FCM)观察药物作用后细胞凋亡及细胞周期的变化-结果:MTT结果显示经GW9662作用后,尼美舒利对SGC-7901细胞增殖的抑制作用减弱,且在一定范围内呈剂量依赖性;FCM检测结果显示GW9662能抑制尼美舒利的促细胞凋亡作用,使G0/G1期细胞比例降低,S期细胞比例升高-结论:PPARγ途径很可能是选择性COX-2抑制剂尼美舒利影响胃癌细胞增殖及凋亡的途径之一-

摘要:目的:检测卵巢过度刺激综合征(OHSS)大鼠体内IL-1-IL-6-IL-10的变化,探讨OHSS的发病机制-方法:将Wistar雌性清洁大鼠随机分成生理盐水组和OHSS组-卵巢过度刺激组皮下注射孕马血清促性腺激素(PMSG)10 IU,每日1次,连续4天,第5天皮下注射100 IU人绒毛膜促性腺激素(HCG)一次建立OHSS模型;对照组皮下注射生理盐水每天1次,连续5天-HCG注射后48 h,取大鼠血-卵巢测IL-1-IL-6-IL-10(酶联免疫法)-结果:OHSS组血和卵巢IL-1均高于对照组,有显著差异(P < 0.05);两组血IL-6-IL-10含量极低,无法测出-OHSS组卵巢IL-10含量显著降低,有统计学意义(P < 0.05)-结论:大鼠OHSS全身症状可能与主要由IL-1介导的炎症相关,而IL-6和IL-10参与卵巢局部炎症-

摘要:目的:运用RNA干扰(RNA interference,RNAi)技术阻断胰腺癌细胞株PANC-1中NF-κB P65基因表达,并研究该基因沉默后对细胞增殖与凋亡的影响-方法:利用阳离子脂质体LipofectamineTM2000将化学合成的人NF-κB P65的小干扰RNA转染入胰腺癌细胞株PANC-1中-RT-PCR法测定胰腺癌细胞内NF-κB P65mRNA与细胞周期素D1(cyclinD1)mRNA的表达-ELISA法检测NF-κB亚单位P65的DNA结合活性的改变-MTT法测定细胞生长曲线观察细胞增殖的抑制情况-流式细胞仪测定细胞凋亡率及细胞周期的变化-结果:化学合成的人NF-κB P65 siRNA能有效地抑制PANC-1细胞中NF-κB P65与cyclinD1基因在mRNA水平上的表达(P < 0.01),同时ELISA结果显示,RelA siRNA组的p65亚单位与DNA结合活性明显低于阴性对照组和空白对照组(P < 0.05)-RelA siRNA组中细胞增殖减慢,凋亡率较对照组明显增加(P < 0.01),同时G1期细胞所占的比例较对照组增加了10%,S期和G2/M期细胞则分别减少了4%与6%-结论:体外实验初步证明NF-κB P65基因在胰腺癌细胞株增殖分化与凋亡方面扮演重要角色,通过沉默其表达可抑制胰腺癌细胞株PANC-1增殖并诱导其凋亡-

摘要:目的:观察慢性粒细胞白血病(CML)初发和急变患者外周血BCR/ABL融合基因和转录因子FoxO3a的表达变化及其意义-方法:利用EVA Green建立实时荧光RT-PCR检测 BCR/ABL-FoxO3a和GAPDH的方法,检测25例CML初发和25例CML急变患者的外周血样本中BCR/ABL融合基因和FoxO3a的表达,以及30例正常人外周血样本中FoxO3a的表达,以GAPDH为内参基因,各组基因表达水平的差异采用△△CT相对定量法-结果:CML急变患者外周血BCR/ABL融合基因的表达水平是初发患者的4.72倍,正常人外周血样本中FoxO3a的表达水平分别是CML初发和急变患者的143.39倍和8.18倍-结论:BCR/ABL融合基因的高表达与转录因子FoxO3a的低表达可能与CML不同阶段的病理过程相关联-

摘要:目的:观察生长抑素(SST)或肠血管活性多肽(VIP)对大鼠肠淋巴细胞归巢肠相关淋巴组织的影响并初步探讨其作用机制-方法:VIP或SST体外孵育肠淋巴细胞,51Cr 标记细胞后回输入大鼠体内,测定51Cr-细胞在肠相关淋巴组织(GALT)的数量-RT-PCR测定肠淋巴细胞SSTR-1~5和VIPR-1~2mRNA的表达-结果:经SST或VIP孵育的淋巴细胞在Peyer淋巴结数量较对照组减少,其在小肠弥散淋巴组织的数量较对照组无显著改变-肠淋巴细胞表达SSTR3和VIPR2基因-结论:生理状态下,SST或VIP抑制肠循环淋巴细胞归巢GALT,降低肠黏膜免疫功能,这一作用可能是通过SSTR-3和VIPR-2实现的-

摘要:目的:建立从血清样品直接扩增乙型肝炎病毒(HBV)全长DNA的方法,构建HBV全基因组克隆-方法:用长链PCR从血清样品一次性扩增HBV全长DNA,测序鉴定后插入质粒puc19构建含HBV全基因组的重组质粒,再从重组质粒扩增X基因并进行序列分析-结果:经DNA序列测定和酶切分析,获得HBV全基因组克隆,从克隆的HBV全基因组扩增到X基因-结论:从血清中可直接扩增到HBV全基因组DNA,为整体水平研究HBV基因组的变异与其致病及宿主抗感染免疫之间的关系奠定了实验基础-

摘要:目的:比较产前给药氨溴索和糖皮质激素(地塞米松-倍他米松)对大鼠胎肺形态发育的影响-方法:12只孕鼠随机分成四组:生理盐水对照组,氨溴索组,地塞米松组,倍他米松组-通过光镜观察-图像分析及电镜观察比较各组孕鼠的胎仔肺组织形态发育差异-结果:光镜下各治疗组呼吸膜周径大于对照组(P < 0.05),肺泡表面积大于对照组(P < 0.01),肺泡间隔均低于对照组(P < 0.01)-倍他米松组与地塞米松组相比肺泡间隔小(P < 0.01),呼吸膜周径及肺泡表面积大(P < 0.01)-透射电镜下各治疗组肺泡Ⅱ型上皮内可见板层小体,细胞器多见,倍他米松组和地塞米松组上述改变尤为明显;而对照组内未见板层小体-细胞器少见-结论:氨溴索-地塞米松-倍他米松均能促进肺发育,且倍他米松效果优于地塞米松-氨溴索无激素用药的毒副作用,可能成为产前促进胎肺成熟的更好的选择-

摘要:目的:研究单唾液酸四已糖神经节苷脂(GM-1)对脑缺血再灌注后诱导型一氧化氮合成酶(iNOS)的影响并探讨其可能机制-方法:采用线栓法制作大鼠大脑中动脉闭塞模型,缺血2 h再灌注22 h-将雄性SD大鼠随机分成假手术组,模型对照组和GM-1处理组(15-30-60 mg/kg 3个给药剂量组)-通过红四氮唑(TTC)染色和图像分析计算各组脑梗死体积,采用生物化学法测量各组伤侧脑中NO含量和iNOS活性-结果:与模型对照组相比,GM-1中-高剂量处理组脑梗死体积缩小,iNOS活性和NO量下降,低剂量组变化不明显-结论:脑缺血后iNOS升高与脑损伤关系密切,而GM-1可以对抗iNOS和NO上升,并减轻脑缺血损伤-

摘要:目的:通过观察持续气道正压通气(CPAP)对合并难治性高血压(RH)的阻塞性睡眠呼吸暂停综合征(OSAS)患者的血压和血浆醛固酮(ALD)-血浆肾素活性(PRA)的影响,以探讨CPAP治疗对OSAS合并RH患者血压的疗效及可能的机制-方法:选择13例合并RH的中重度OSAS患者,分别于CPAP治疗前及CPAP治疗3个月时测量和比较睡前(10:00PM)-夜间(02:00AM)和清晨(6:00AM)的血压及晨起(6:00AM)卧位血浆ALD-PRA水平,ALD-PRA检测采用放射免疫分析法-结果:与CPAP治疗前相比,CPAP治疗3个月时相比变化如下:睡前-夜间和清晨血压均显著下降(均为P < 0.01),其中收缩压比较为10:00PM时:(152.2 ± 19.2)mmHg vs(135.5 ± 2.8)mmHg,2:00AM时:(156.6 ± 19.4)mmHg vs(133.1 ± 2.4)mmHg,6:00AM时:(172.1 ± 23.7)mmHg vs(151.5 ± 3.0)mmHg;舒张压比较为10:00PM时:(98.9 ± 15.6) vs(84.2 ± 4.6)mmHg,2:00AM时:(102.8 ± 16.6)mmHg vs(81.5 ± 4.6)mmHg,6:00AM时:(50.9 ± 16.9)mmHg vs(50.7 ± 4.3)mmHg;晨起卧位ALD明显降低[(195.5 ± 15.1)ng/L vs(148.3 ± 19.4)ng/L,P < 0.01],但PRA无明显变化[(0.20 ± 0.12)μg/(L·h) vs(0.27 ± 0.14)μg/(L·h), P > 0.05)-结论:CPAP治疗可显著改善OSAS患者的RH并降低血浆ALD浓度-OSAS患者的RH可能与血浆ALD浓度增高有关-

摘要:目的:探讨系统性红斑狼疮(SLE)患者血清凋亡调节蛋白sFas和可溶性细胞间黏附分子(sICAM-1)对疾病活动性及治疗转归的预测价值-方法:采用酶联免疫吸附试验(ELISA)检测SLE患者和正常人血清sFas和sICAM-1的水平,分析sFas和sICAM-1的含量与SLE患者的疾病活动度指数SLEDAI和免疫指标(ANA-dsDNA等)的相关性,研究用sFas和 sICAM-1指标判断疾病的活动性,并进行了SLE病例的前瞻性和治疗前后对照研究-结果:SLE患者血清sFas和sICAM-1平均水平分别为4.18 -滋g/L和464.5 -滋g/L,显著高于正常对照组2.27 -滋g/L和190.4 -滋g/L-SLE患者血清sFas和sICAM-1水平活动期均高于非活动期(P < 0.001),ANA高滴度组高于ANA低滴度组,dsDNA抗体阳性组高于dsDNA抗体阴性组,肾损害组高于非肾损害组;且SLE患者血清sFas和sICAM-1水平与SLE患者SLEDAI呈正相关(r = 0.688和r = 0.728)-以非活动性SLE组的x ± s作为评判临界值,同时定量检测sFas( > 4.45 -滋g/L)-sICAM-1( > 438.6 -滋g/L)对SLE活动性的预测具有较好的灵敏度-特异度-结论:sFas和 sICAM-1与SLE发病有关,可反映SLE患者的疾病活动性,同时定量检测可有助于预测患者的病情活动和转归-[关键词] 系统性红斑狼疮; sFas; 可溶性细胞间黏附分子; SLEDAI

摘要:目的:观察急性心肌梗死延迟PCI术后自体骨髓单个核细胞移植对心肌灌注及左室功能的影响-方法:20例急性心肌梗死患者在病情稳定后7~10天,行延迟PCI术-随机分成骨髓细胞移植组(10例)和对照组(10例),观察术前-术后1个月-术后6个月左心室舒张末内径(LVDd),左室射血分数(LVEF)-以及术前-术后1个月的经静脉心肌超声造影(IMCE)-结果:移植组LVEF由术前(37.26 ± 4.21),1月后上升到(54.42 ± 5.26)(P < 0.05);而对照组差异不显著[(38.86 ± 4.63) vs (40.28 ± 4.56)](P > 0.05)-LVDd移植组术后6个月与术前比较差异不显著[(50.23 ± 3.42) vs (52.48 ± 3.26)](P > 0.05);而对照组[(50.96 ± 2.68) vs (64.31 ± 3.28)](P < 0.05),心脏有扩大趋势-经静脉心肌超声造影(IMCE), A·β(dB/s)移植组由术前的(2.37 ± 0.16)增加到术后的(15.60 ± 0.24);较对照组(2.06 ± 0.12至7.98 ± 0.23)增加更为明显(P < 0.05)-结论:急性心肌梗死延迟PCI术后自体骨髓单个核细胞移植可改善患者左室收缩功能,延缓左室扩大;可改善梗死区心肌灌注,明显增加心肌血流量-

摘要:目的:观察银杏叶提取物对椎基底动脉供血不足(VBI)患者的临床疗效-方法:VBI患者96例随机分为治疗组(60例)和对照组(36例)-治疗组采用银杏叶提取物注射液治疗,对照组采用脉络宁注射液治疗,疗程均为14天-观察临床疗效,治疗前后凝血酶原时间(PT)-血纤维蛋白原(Fbg)-血脂水平-血液流变学指标及经颅多普勒超声(TCD)所示血流速度的变化-结果:治疗14天后治疗组总有效率86.7%,对照组总有效率69.4%,两组比较差异显著(P < 0.05) -治疗组红细胞压积-血浆黏度-Fbg-胆固醇及甘油三脂均显著低于治疗前及对照组,PT与治疗前及对照组相比明显延长(P均 < 0.05)-TCD显示治疗组椎动脉及基底动脉收缩期平均血流速度与治疗前及对照组相比明显增快(P < 0.05)-结论:银杏叶提取物治疗VBI疗效确切,值得临床推广应用-

摘要:目的:研究银杏提取物对大鼠缺血再灌注的心肌保护作用及其作用机制-方法:Wistar大鼠先用银杏提取物(0.1 mg/kg)预处理,1 h后拮扎冠状动脉左前降支50 min,之后再灌注5 h-用氯化三苯四唑(TTC)染色测量梗死灶的大小-用电泳迁移率变动分析法(EMSA)分析Stat的活化-Coll样受体(TLLR)和MyD86的结合用免疫印记法进行分析-结果:银杏提取物的处理使再灌注5 h鼠的心肌梗死面积降低58.1%-当银杏提取物在心肌缺血后10 min给药时也被观察到了相同的保护作用-银杏提取物诱导心肌保护的机制包括降低TLLR和MyD86的结合,降低了Stat的活性-并使得I/R后心肌细胞凋亡数目减少-结论:提示了银杏提取物能够对大鼠在体缺血再灌注的心肌起到保护作用并且这种保护作用可能与抑制TLLR介导Stat信号传导途径激活途径相关,后者在心肌I/R损伤中起着重要作用,并促使心肌细胞凋亡的减少-

摘要:目的:测定大肠癌患者外周血血浆游离DNA中含表皮生长因子和卵泡抑素结构域的跨膜蛋白(TPEF)基因启动子区异常甲基化率及K-ras基因12密码子突变率,评估联合检测此二个分子标记非损伤性筛选和诊断大肠癌的可行性-方法:提取大肠癌患者外周血血浆DNA,采用突变富集PCR-RFLP法检测K-ras基因突变,甲基化特异性PCR法检测TPEF基因甲基化异常-结果:42例大肠癌患者血浆标本中,27例检测到TPEF甲基化异常,16例检测到K-ras基因12密码子突变,联合二个分子标记,大肠癌患者诊断符合率为76.2%-结论:联合检测K-ras-TPEF基因异常将可能是一种非损伤性筛选和诊断大肠癌的有效方法-

摘要:目的:探讨宫颈癌组织中survivin基因表达的特征及其临床意义-方法:应用RT-PCR及免疫组化方法检测survivin基因在64例宫颈癌组织-17例不典型增生(CIN)及14例正常宫颈组织中的表达,分析其与临床病理特征的相关性-结果:RT-PCR显示宫颈癌组织中survivin基因的mRNA水平显著高于正常宫颈组织,并且随宫颈癌的病理分级增高而增高(P < 0.05)-SP法显示宫颈癌组织中survivin基因的表达较正常组织及CIN均明显升高(P < 0.05);且与宫颈癌的病理分级及临床分期有关-结论:survivin基因在宫颈癌中异常表达与宫颈癌的发生密切相关,可以作为反映宫颈癌恶性潜能的一项新指标-

摘要:目的:探讨DNA倍体-PCNA表达与放射敏感细胞对宫颈癌的诊断价值,分析和评价3项指标对选择放疗及判定疗效的意义-方法:46例临床拟诊为宫颈癌的患者放疗前测DNA倍体-PCNA表达,行细胞学和病理学检查确诊,于放疗中-后测DNA倍体-PCNA表达和细胞学-病理学检查,观察宫颈细胞的放射反应变化和判断放疗效果-结果:DNA倍体的异倍体量与宫颈癌的异型性和放射敏感性成正相关;PCNA表达与宫颈癌病理分级和放射敏感性成正相关-细胞学放疗前-后诊断与病理学对比分析,其敏感性为100%-特异性为95.65%,其敏感细胞和反应细胞能客观反映宫颈癌的放疗敏感程度和放疗效果-结论:DNA倍体-PCNA表达与细胞学检查可以作为宫颈癌定性和分级诊断的客观依据,可以作为选择放疗和判定疗效的客观指标,三者联合应用对宫颈癌的诊断与判定疗效意义更大-

摘要:目的:对于那些FOLFOX治疗失败的转移性结直肠患者使用周剂量CPT-11联合5-FU/LV挽救化疗,观察治疗效果和不良反应-方法:入组患者共24例为转移性结直肠癌,经草酸铂及5-FU/LV治疗失败后,予CPT-11 60 mg/m2,LV100 mg/m2, 5-FU 500 mg/m2,每周1次,连续3周,每4周重复为1个疗程,患者最多接受6个疗程-结果:全组病例均可评价疗效及不良反应-PR为3例,SD为13例,有效率12.5%(95%可信区间3.3%~32.4%),疾病控制率66.7%(95%可信区间47.8%~85.6%),平均TTP为3.5个月(范围1.5~7.5个月)-结论:周剂量伊立替康联合5-FU/LV为草酸铂治疗失败的转移性结直肠癌挽救治疗方案,不良反应轻微,疗效与FOLFIRI相当,适合于先前强烈化疗患者-

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摘要:目的:探讨1,25(OH)2D3 对大鼠胰腺移植急性排斥反应的免疫调控作用-方法:以F344大鼠为供体,Lewis大鼠为受体,分A组(急性排斥对照组);B组(雷帕霉素治疗组);C组[1,25(OH)2D3治疗组],每组15例;观察术后各组血糖变化,移植物存活以及组织病理学改变;流式细胞检测受体血-脾以及移植物CD4+T细胞-CD8+T细胞-CD4+CD25+ T细胞表达水平;ELISA检测受体血清IL-2-4-10-12表达水平;在诱导Lewis大鼠骨髓细胞定向分化为树突状细胞(DC)时加入1,25(OH)2D3,流式细胞仪检测CD80,CD86及MHCⅡ类分子表达情况-结果:预防性使用1,25(OH)2D3-保护移植物功能(血糖5.12 ± 0.89 VS 12.41 ± 1.16),延长移植后受体平均存活时间;CD8+T细胞计数C-B与A组相比均有减少,差异有统计学意义(P < 0.01)-CD4+T细胞C-B与A组的差异有统计学意义-CD4+CD25+调节性T细胞A-B-C组逐渐升高,有统计学意义(P < 0.01),且以C组较为明显(C组 VS A组P < 0.01);血清Th1类细胞因子浓度明显降低,Th2类细胞因子浓度明显增高;在体外实验中,1,25(OH)2D3显著抑制了DC的成熟过程及其表面共刺激分子的表达-结论:1,25(OH)2D3可抑制DC发育成熟,诱导产生基因耐受性树突细胞,促进调节性T淋巴细胞的增生,抑制致病性T淋巴细胞,抑制急性排斥反应,延长受体存活时间-

摘要:目的:探讨虾青素对去卵巢骨质疏松模型大鼠股骨的密度-骨矿含量的影响及机制-方法:将72只15周龄SD大鼠随机分为6组,空白组做假手术,其余5组做卵巢切除术,术后用药12周后处死,测定右侧股骨骨密度-骨矿含量-血清雌二醇-碱性磷酸酶含量及子宫湿重-结果:虾青素高-中-低剂量(20-15-10 mg/kg)组-尼尔雌醇(1.05 mg/kg)组能对抗骨密度-骨矿含量-血清雌二醇的下降-血清碱性磷酸酶的升高(与模型组相比P < 0.05);各剂量组-尼尔雌醇组能对抗子宫指数的减轻(与模型组相比P < 0.05)-结论:虾青素可以改善骨质量,抑制大鼠去卵巢骨质疏松的发生,具有类似雌激素的效应-

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摘要:目的:探讨多排螺旋CT对肺组织灌注成像合适的注射速率-方法:研究采用配对设计,处理因素为对比剂的注射速率,分别为3-4-5-6 ml/s,每组15例志愿者随机入组,分成4组共60例进行灌注扫描,所得图像经后处理测量肺门层面的两肺组织对比剂至峰值时间及峰值-结果:注射速率对两肺组织至峰值时间有显著性影响-其中注射速率为5 ml/s-6 ml/s组肺组织至峰值时间及峰值无统计学显著性差异(P > 0.05),3 ml/s-4 ml/s两组与5 ml/s-6 ml/s两组依次相比较肺组织至峰值时间延长,峰值降低-结论:注射速率≥5 ml/s时肺组织灌注成像可获得满意效果-

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摘要:目的:调查某医科大学在校学生中打鼾分布情况及相关危险因素-方法:采用整群抽样的方法抽取2001级11个班级中的328名同学进行问卷调查和体格检查,并对部分相关因素进行分析-结果:收到问卷328份(应答率100%),有效问卷328份(有效率100%)-其中打鼾者占36.6%,经常打鼾者占8.2%,男性高于女性(P < 0.05),打鼾的危险因素有肥胖(P < 0.05)-悬雍垂过度(P < 0.05)-颈围(P < 0.05),而仰卧会加重打鼾程度(P < 0.05);父母打鼾情况-吸烟-饮食-运动-心理状况分布未见有统计学差异-结论:大学生打鼾流行率为36.6%,打鼾影响大学生健康的重要问题之一,应当引起重视-

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