• 2008年第28卷第5期文章目次
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    • >南京医科大学学报(英文版)
    • Very small embryonic like(VSEL) stem cells

      2008, 28(5):265-268. DOI: 10.7655

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      摘要:It is generally accepted that adult bone marrow(BM) contains both hematopoietic stem cells(HSCs) and mesenchymal stem cells (MSCs). Recently, a rare population of stem cells different from HSCs and MSCs were identified in murine BM and human cord blood (CB), named as very small embryonic like(VSEL) stem cells. These cells are tiny round and CXCR4+ Sca-1+ Lin- CD45-, expressing SSEA-1/4, Oct-4 and Nanog, which have potent of differentiation into all three germ-layer lineages, such as cardiomyocytes, neural and pancreatic cells.

    • Dosimetry for 125I radioactive seed implantation therapy for hepatocellular carcinoma

      2008, 28(5):269-272. DOI: 10.7655

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      摘要:Hepatocellular carcinoma (HCC) is an aggressive malignancy. Early lesions respond well to hepatic resection or liver transplantation. However, only a few of HCC patients are suitable for surgical intervention. External beam radiation and chemotherapy is poorly efficacious. In the last 20 years, HCCs belonging to the radiosensitive tumor group has been confirmed. Along with the development of new radiotherapy technology and facilities, the research about brachytherapy(especially 125I seed implantation therapy) has provoked more interests in the world. Radioactive seed implantation therapy is a form of interstitial brachytherapy, with the property of local “conformal radiotherapy” and the advantages of minimal invasion, convenience, high performance, and minimal adverse effects. It is a promising therapy for HCC, however the dosimetry hasn′t yet been identified and lacks verification in prospective research. This report aims to further explore the best prescription dose and radioactivity for 125I interstitial implantation brachytherapy for HCC.

    • Comparison between two commercial immunoassays:Dr. Fooke ALLERG-O-LIQ versus Phadia ImmunoCAPR System in detecting allergen-specific IgE and total IgE values

      2008, 28(5):273-278. DOI: 10.7655

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      摘要:Objective: Beside patient’s history and skin prick testing(SPT) the detection of specific IgE(sIgE) represents an important tool of allergy diagnostics. In recent years different technologies for the detection of sIgE have been developed. The objective of this study is the comparison of the ALLERG-O-LIQ with the ImmunoCAPR System using seven inhalant and four food allergens. Methods: Sera from patients were collected and tested for sIgE to inhalant(d1, d2, d5, i6, e1, e5 and m3) and food allergens(f1, f2, f24, f24) by ALLERG-O-LIQ(Dr. Fooke Laboratorien GmbH) and by ImmunoCAPR System(Phadia). Further, samples were also tested for total IgE in both systems. Results: Prevalence of positive test results varied between 0/20(f24) and 11/20(e5) for ALLERG-O-LIQ and between 3/18(f23) and 11/20(d1/d5) for ImmunoCAPR. The qualitative agreement between both methods was found between 75%(f24) and 100%(d2) depending on the allergen. Overall qualitative agreement for inhalant(n = 140), food(n = 78) and all allergens(n = 218) tested was 92.1%(kappa = 0.84), 83.3%(kappa = 0.58), 89.0%(kappa = 0.77), respectively. Sensitivity, specificity, positive predictive value(PPV), negative predictive value(NPV) and diagnostic efficiency(DE) were found at 88.2%, 95.8%, 95.2%, 89.6%, 92.1%(inhalant allergens), 62.5%, 92.6%, 78.9%, 84.7%, 83.3%(food allergens) and 81.5%, 94.4%, 91.5%, 87.5%, 89.0%(all allergens). Conclusion: Good to excellent qualitative agreement between ALLERG-O-LIQ and ImmunoCAPR for the detection of specific and total IgE could be observed. The degree of agreement depended on the allergen and was higher in the group of inhalant allergens. The ALLERG-O-LIQ System represents a reliable test for the detection of specific and total IgE.

    • P53 codon 72 polymorphism and ovarian cancer risk: a meta-analysis

      2008, 28(5):279-285. DOI: 10.7655

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      摘要:Objective: p53 is a tumor suppressor gene and is involved in the etiology of ovarian cancer. Studies investigating the associations between the p53 codon 72 polymorphism and ovarian cancer risk showed conflicting results. We performed this meta-analysis from eligible studies to evaluate this purported relationship. Methods: This meta-analysis was performed from 9 case-control studies, including 825 ovarian cases and 1073 controls. The fixed and random effect models were used to estimate the odds ratios(ORs) for various contrasts of this polymorphism. Results: The combined results based on all studies showed that a significantly decreased risk was associated with the variant Pro/Pro genotype, compared with Arg/Pro+Arg/Arg genotypes(OR, 0.70; 95%CI, 0.51~0.95). When stratifying the studies by ethnicity, we found that individuals with the variant genotype Pro/Pro had a significantly decreased risk of ovarian cancer compared with Arg/Arg genotype(OR, 0.43; 95%CI, 0.20~0.89) and Arg/Pro+Arg/Arg genotypes(OR, 0.61; 95%CI, 0.37~0.99) among Africans. Conclusion: This meta-analysis suggests that the p53 codon 72 polymorphism may contribute to genetic susceptibility to ovarian cancer. More studies based on larger sample size should be performed to confirm the findings.

    • Using SELDI-TOF-MS technology for screening serum markers of hepatic carcinoma in rats

      2008, 28(5):286-289. DOI: 10.7655

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      摘要:Objective: To identify potential serum markers of hepatic carcinoma in rats through Surface-Enhanced Laser Desorption Ioniza-tion-Time of Flight-Mass Spectrometry(SELDI-TOF-MS) Technology. Methods: A rat model of hepatic carcinoma was established. The serum samples of hepatic carcinoma and normal rats were analyzed via SELDI-TOF-MS Technology. The changes of the serum protein fingerprint patterns were observed between the experimental group of hepatic carcinoma and the controls. The analysis was conducted by statistical software-Biomarker Wizard. Results: Fifty-six protein peaks in the serums were found. Within m/z 0-20 000, the protein peaks of m/z 1158, 8 835 and 15 302 of hepatic carcinoma serums were obviously higher in the rat models compared with those in the controls(P < 0.01). Conclusion: Three peaks were considered as potential biomarkers according to the serum protein fingerprint patterns of the hepatic carcinoma group and the control group.

    • Effect of behavior training on learning, memory and the expression of NR2B, GluR1 in hippocampus of rats offspring with fetal growth restriction

      2008, 28(5):290-294. DOI: 10.7655

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      摘要:Objective: To study effects of behavior training on learning, memory and the expression of NR2B, GluR1 in hippocampus of rat’s offspring with fetal growth restriction(FGR). Methods: The rat model of FGR was established by passive smoking method. The rats offspring were divided into the FGR group and the control group, then randomly divided into the trained and untrained group, respectively. Morris water maze test was proceeded on postnatal month(PM2/4) as a behavior training method, then the learning-memory of rats was detected through dark-avoidance and step-down tests. The expressions of NR2B and GluR1 subunits in hippocampal CA1 and CA3 areas were detected by immunohistochemical method. Results: In the dark-avoidance and step-down tests, the performance record of rats with FGR was worse than that of control rats, and the behavior-trained rats was better than the untrained rats, when the FGR model and training factors were analyzed singly. The model factor and training factor had significant interaction(P < 0.05). The expressions of NR2B and GluR1 subunits in hippocampal CA1 and CA3 areas of rats with FGR reduced. In contrast, the expressions of GluR1 and NR2B subunits in CA1 area of behavior-trained rats increased, when the FGR model and training factors were analyzed singly. Conclusion: These findings suggested that the effect of behavior training on the expressions of NR2B and GluR1 subunits in CA1 area should be the mechanistic basis for the training-induced improvement in learning-memory abilities.

    • The evaluation of Tracp5b as a marker for monitoring treatment results of bone metastasis in breast cancer patients

      2008, 28(5):295-298. DOI: 10.7655

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      摘要:Objective:To evaluate the sensitivity of serum tartrate-resistant acid phosphatase 5b(Tracp5b) activity in monitoring bisphosphonate treatment results of bone metastasis in breast cancer(BC) patients. Methods:The serum activities of Tracp5b, CEA, CA153 were mea-sured in 58 BC patients, including 26 without bone metastasis, 32 with bone metastasis. The serum activities of Tracp5b, CEA, CA153 were also measured in 19 patients with bone metastasis after 3 months of bisphosphonate treatment. Eighteen healthy women with age from 34 to 70 served as control. Results:Serum Tracp5b was significantly elevated in patients with bone metastasis compared with that in all any other groups(P < 0.05). The sensitivity of Tracp5b was 78.13% and the specificity was 86.36%. The sensitivity of CA153 was 37.50% and the specificity was 77.27%. The sensitivity of CEA was 21.88% and the specificity was 84.09%. The serum activity of Tracp5b decreased significantly(P < 0.05) after 3 months of bisphosphonate treatment, while the levels of CA153 and CEA were unchanged. Conclusion:Serum Tracp5b activity is a useful diagnostic marker for bone metastasis in BC patients and can be used to evaluate the treatment results of bisphosphonate.

    • Effect of ecdysterone on heteroptopic heart transplantation in rats

      2008, 28(5):299-303. DOI: 10.7655

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      摘要:Objective: To investigate the protective effects of ecdysterone(EDS) on the allograft heart transplant model of rats. Methods: Fifty healthy Sprayue-Dawley(SD) rats were divided into donors and recipients randomly. Hearts were harvested and placed heterotopically into allogenic recipients. All animals were divided into three groups: sham-operation group(only opening and closing the abdomen, n=10), EDS group(injected intraperitoneally with 20 mg/(kg·day) of EDS, n = 10), and control group (injected intraperitoneally with normal saline, n = 10). The pathological changes of myocardial tissue were analyzed by light microscopy and transmission electron microscopy and the levels of myocardial enzymes(GOT, LDH, CPK), SOD, ET-1, NO, MDA in serum were measured. Tissue samples underwent the detection of apoptotic cell death by in situ terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling for apoptotic index(AI) and also by immunohistochemistry method to study the expressions of Bcl-2 and Bax. Results: Pathological examination and transmission electron microscope observation showed greater myocardium damage in the control group. EDS group showed a decrease in the amount of myocardial enzymes, MDA, ET-1 and an increase in the levels of SOD, NO, compared to the control group. The AI of EDS group became lower than that of control group, meanwhile the EDS group increased the expression of Bcl-2 and decreased the expression of Bax. Conclusion: EDS has protective effects on heteroptopic heart transplantation, which provides a new idea for myocardial protection in heart transplantation. However, the mechanism of its protective effect needs further research.

    • Construction and identification of recombinant lentivirus-mediated gene transfer system for rat transducer of regulated CREB activity 1

      2008, 28(5):304-307. DOI: 10.7655

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      摘要:Objective: To construct a recombinant lentivirus vector which carries SD rat transducer of regulated CREB activity-1(TORC1) gene and examine its ability to express the TORC1 gene in vitro. Methods: The coding sequence of SD rat TORC1 gene was amplified using PCR and cloned into pGC-FU vector. 293T cells were transfected using Lipofectamine 2000 and packaged for the recombinant lentivirus particles. When the cloned sequence was identified to be right, the recombinant lentivirus particles were amplified in a large quantity. The titer of virus was determined by real-time PCR and the level of TORC1 expression was examined by Western blot. Results: The recombinant lentivirus vector carrying TORC1 was constructed successfully and could express TORC1 at a high level in 293T cells in vitro, and the titer determined by real-time PCR was 2×108 TU/ml. Conclusion: The recombinant lentivirus vector could express TORC1 gene at a high level, and was very helpful in the study of exploring the effect of TORC1 on spinal cord injury.

    • An animal model to create intervertebral disc degeneration characterized by radiography and molecular biology

      2008, 28(5):308-312. DOI: 10.7655

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      摘要:Objectives: To develop a rabbit model of intervertebral disc degeneration that more exactly simulates the pathological changes of human intervertebral disc degeneration. Methods: Twelve New Zealand white rabbits were utilized to establish three different disc injury models according to the following protocol; group A: anulus punctures were done with a 18-gauge needle at L2-L3 and L5-L6; Group B: intradiscal injection of interleukin-1 IL-1βwith a 23-gauge needle at L3-L4; and Group C: intradiscal injection of phosphate buffer saline(PBS) with a 23-gauge needle at L4-L5. The L1-L2 level was used as a control. Rabbits were killed after 24 weeks. The intervertebral disc height was measured by lateral plain radiographs. After the radiographic measurements were obtained, the interver-tebral discs were removed and analyzed for DNA, sulfated glycosaminoglycan(s-GAG) and water contents of nucleus pulposus. Results: The intervertebral disc height, s-GAG, and water contents in anulus needle punctures were significantly decreased in Group A, but the DNA content in the nucleus pulposus was significantly increased when compared to the control. The significant decrease of disc height and water contents were demonstrated, only the s-GAG and DNA contents did not show a significant difference in Group B when compared to the control. The significant decrease of disc height, s-GAG, water, and DNA contents did not show in Group C when compared to the control. Conclusion: The 18-gauge puncture models produced the most consistent disc degeneration in the rabbit lumbar spine.

    • A Streptococcus suis serotype 2 caused streptococcal toxic shock syndrome(STSS) in a patient

      2008, 28(5):313-316. DOI: 10.7655

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      摘要:Streptococcus suis (S. suis) is a Gram-positive, facultatively anaerobic coccus that has been implicated as the cause of a wide range of clinical disease syndromes in swine and other domestic animals. S. suis has also been implicated in disease in humans, especially among abattoir workers, swine and pork handlers. Here we report a case of streptococcal toxic shock syndrome(STSS) caused by S. suis in a 59-year-old man. Despite of intensive treatment, the patient died of shock with multiple organ failure 14 h after admission. One bacterial isolate obtained from blood culture was identified to the species level by biochemical tests and serological tests as S. suis serotype 2. Identification was confirmed by PCR amplification of genes encoding 16sRNA of S. suis and the capsule of S. suis serotype 2(cps 2J). Genes encoding virulence factors were also detected. An investigation to identify the source of S. suis revealed that several days before admission the affected man had been handling sick pigs or their meat. Transmission may occur through breaks in the skin of feet with tinea due to that no measures for personal protection was taken. This case should highten awareness of the potential for occupational exposure and human infection with S. suis.

    • Asthma-like tracheobronchial amyloidosis: a case report and review of the literature

      2008, 28(5):317-320. DOI: 10.7655

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      摘要:Tracheobronchial amyloidosis(TBA) is a rare disorder which refers to localized aberrant protein deposits within the airways. Because of the lack of characteristic presentation, long lasting and insidious course, patients with TBA are often misdiagnosed as other pulmonary diseases. At present bronchoscopic biopsy was the gold standard for the diagnosis of TBA. We present a case of TBA which was misdiagnosed as asthma due to asthma-like wheezing and the effectiveness of anti-asthmatic treatment. The definite diagnosis was confirmed by CT scan, bronchoscopic findings and Congo red staining of biopsy specimen. The literature involving the recent progres-sion of diagnosis and treatment of TBA that is reviewed.

    • Diagnosis of a case of thymus area lymphoma

      2008, 28(5):321-324. DOI: 10.7655

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      摘要:This was a rare case, in which clinical manifestation was a thymus area tumor accompanied by proteinuria, kidney dysfunction and kidney enlargement. Tumor biopsy and bone marrow examination failed to clarify its diagnosis. Renal biopsy revealed diffuse infiltra-tion by atypical lymphoid cells in renal interstitium. Immunoperoxidase studies demonstrated this case was non-Hodgkin’s lymphoma(NHL) of the B cell type through the renal biopsy.

    • Benign symmetric lipomatosis of the knees

      2008, 28(5):325-327. DOI: 10.7655

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      摘要:Benign symmetric lipomatosis(BSL) is a rare disease characterized by the presence of multiple, symmetric and nonencapsulated fat masses in the face, neck and other areas. It is commonly seen in middle-aged Caucasian Mediterranean males, while its etiology is still not clear. The majority of the patients with BSL have a history of alcohol abuse and hepatopathy. BSL of the limbs is very rare. This article reports a unique case of a 60-year-old Chinese woman with involvement of the knees confirmed by the results of magnetic resonance imaging(MRI) and histopathology, which was not described previously in published literatures.

    • >南京医科大学学报(自然科学版)
    • 人肝细胞癌Huh7细胞中EP3受体不同亚型的鉴定及相关胞内信号转导通路的研究

      2008, 28(5):561-564569. DOI: 10.7655

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      摘要:目的:鉴定人肝细胞癌Huh7细胞中EP3受体不同亚型的类型及其介导的细胞内信号转导途径,探讨EP3对人肝细胞癌发生发展的影响及相关分子机制-方法:用RT-PCR实验检测Huh7细胞中EP3亚型的类型,给予EP3受体激动剂sulprostone处理后,用WST-8法检测细胞的增殖活性,用酶联免疫法测定细胞内环磷酸腺苷(cAMP)浓度的变化,用Western blotting法检测Akt-Erk等蛋白磷酸化水平的变化-结果:RT-PCR结果显示人肝细胞癌Huh7细胞中表达的EP3剪接体类型是EP3(5);WST-8实验结果显示,经10.0 μmol/L EP3受体激动剂sulprostone作用24 h后,细胞增殖率增加46.2%;酶联免疫法显示sulprostone可促使细胞内cAMP浓度显著增高;Western blotting实验显示,sulprostone作用30 min可使Huh7细胞中Akt蛋白磷酸化水平增加27%,而Erk蛋白磷酸化水平降低16.4%-结论:人肝细胞癌Huh7细胞中,表达的EP3亚型类型是EP3(5),EP3受体激动剂sulprostone可以促进Huh7细胞的增殖,通过EP3受体的介导促进胞内cAMP表达水平的增加,其机制可能部分涉及到Akt及Erk信号转导通路-

    • HIV-1假病毒的构建及对人原发性渗出性淋巴瘤细胞系的感染研究

      2008, 28(5):565-569. DOI: 10.7655

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      摘要:目的:分离-克隆水泡性口炎病毒(vesicular stomatitis virus,VSV)的包膜糖蛋白(VSV-GP)编码基因,包装人类免疫缺陷病毒1 型(human immunodeficiency virus-1,HIV-1)假病毒,并研究其对人原发性渗出性淋巴瘤细胞系BCBL-1细胞的感染状况-方法:根据GenBank 登记的VSV-GP基因核苷酸序列设计1对PCR 引物,其5′端分别引入HindⅢ和BamHⅠ酶切位点-以质粒pHCMV-G为模板,PCR 扩增VSV-GP 基因,经双酶切后定向克隆进真核表达载体pcDNA3.1(+)中,构建重组真核表达质粒pVSV-GP-重组质粒经酶切鉴定-核酸序列测定和分析后与含包装和复制功能缺陷的HIV-1基因组重组质粒pNL4-3.Luc.R-E-共转染人胚肾HEK 293T细胞,收获细胞进行Western blot,检测HIV-1 p24蛋白的表达;收获HIV-1假病毒感染BCBL-1细胞,进行虫荧光素酶(Luciferase)报告基因活性检测以及用ELISA的方法检测感染后细胞上清中HIV-1 p24蛋白的含量-结果:PCR 分离-克隆的VSV-GP序列全长1 536 bp,序列经过核酸分析证实;Western blot在HIV-1 p24蛋白预期位置检测到特异性条带;HIV-1假病毒感染BCBL-1细胞后上清可以检测到HIV-1 p24蛋白,并且测得Luciferase活性值较对照组显著增高(P < 0.05)-结论:成功包装了HIV-1 假病毒,并且该病毒可以感染BCBL-1细胞-

    • 白藜芦醇对鼠纤维肉瘤S180细胞周期影响及其机制研究

      2008, 28(5):570-574. DOI: 10.7655

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      摘要:目的:研究白藜芦醇对鼠纤维肉瘤S180细胞的抗肿瘤作用,并探讨其分子机制-方法:用MTT法检测白藜芦醇对鼠纤维肉瘤细胞系S180增殖的影响;PI单标流式细胞术检测白藜芦醇对S180细胞周期分布的影响;Western blot方法检测白藜芦醇对S180细胞cyclinD1,P21Cip1蛋白表达的影响-结果:白藜芦醇对S180细胞增殖呈双相作用:低浓度促进细胞增殖;高浓度抑制细胞增殖,其抑制作用呈时效和量效关系-白藜芦醇治疗组S180细胞周期发生变化,细胞周期被阻滞于G0/G1期-白藜芦醇以时间依赖方式下调周期蛋白cyclinD1的表达,上调白藜芦醇P21Cip1蛋白表达-结论:白藜芦醇低浓度促进S180细胞增殖,高浓度抑制细胞增殖-白藜芦醇可通过调节细胞周期蛋白cyclinD1,P21Cip1的表达调控细胞周期进程,抑制细胞增殖-

    • 非小细胞肺癌荷瘤鼠99mTc-acetate-octreotide显像与生长抑素受体表达水平的实验研究

      2008, 28(5):575-579. DOI: 10.7655

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      摘要:目的:探讨非小细胞肺癌(NSCLC)荷瘤鼠模型肿瘤组织中生长抑素受体(SSTR)的表达水平与99mTc-acetate-octreotide显像的相关性研究-方法:体外培养NSCLC细胞株SPC-A1,种植于balb/c裸鼠皮下成瘤,建立人NSCLC荷瘤鼠模型;行99mTc-acetate-octreotide显像,勾画ROI计算肿瘤与对侧正常组织(N/NT)放射性比值,并测定肿瘤及主要脏器单位组织的放射性摄取百分值(%ID/g),采用逆转录-聚合酶反应(RT-PCR)检测肿瘤组织中各SSTR亚型mRNA的表达水平,对SSTR亚型表达水平与T/NT放射性摄取比值进行相关性研究-结果:99mTc-acetate-octreotide NSCLC荷瘤鼠模型显像示肿瘤部位有较高的放射性浓聚,与对侧正常组织T/NT比值较高,4 h达2.38 ± 0.09;99mTc-acetate-octreotide荷瘤鼠体内生物学分布示药物在肿瘤部位有较高的摄取;RT-PCR示NSCLC组织中有着丰富的SSTR表达,SSTR3-SSTR5亚型表达水平较高,其中SSTR5的mRNA表达水平与肿瘤显像阳性荷瘤鼠T/NT比值呈正相关(r = 0.93,P < 0.01)-结论:NSCLC细胞株SPC-A1高表达SSTR3和SSTR5,其中SSTR5表达水平与肿瘤组织对99mTc-acetate-octreotide的摄取呈正相关-99mTc-acetate-octreotide受体显像对NSCLC有较好的影像诊断价值-

    • 非小细胞肺癌中VEGF-C?PDGF-BB与淋巴管生成和淋巴结转移的关系

      2008, 28(5):580-584653. DOI: 10.7655

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      摘要:目的:探讨血管内皮生长因子C(VEGF-C)和血小板衍生生长因子BB(PDGF-BB)在非小细胞肺癌(NSCLC)中的表达与淋巴管生成和淋巴结转移的关系-方法:分别应用免疫组化SP法和Envision System法检测40例NSCLC和10例肺良性病变组织中VEGF-C和PDGF-BB的表达;应用淋巴管特异标志Podoplanin检测NSCLC中淋巴管密度(LVD),并做相关统计分析-结果:VEGF-C和PDGF-BB在NSCLC中阳性表达率均显著高于肺良性病变(VEGF-C:62.5% vs 10%,P < 0.05;PDGF-BB:60% vs 10%,P < 0.05)-淋巴结阳性组的VEGF-C阳性表达率显著高于淋巴结阴性组(94.1% vs 39.1%,P < 0.05),但未发现PDGF-BB表达与淋巴结有关(76.5% vs 47.8%,P > 0.05)-NSCLC中淋巴结阳性组的LVD显著高于淋巴结阴性组(16.58 ± 2.38 vs 9.88 ± 1.93,P < 0.05),VEGF-C和PDGF-BB阳性表达组的LVD均显著高于阴性表达组(VEGF-C:14.74 ± 3.62 vs 9.37 ± 1.35,P < 0.05;PDGF-BB:13.84 ± 4.23 vs 11.06 ± 2.90,P < 0.05)-NSCLC中VEGF-C与PDGF-BB的表达具有显著相关性(rs=0.422,P < 0.05)-结论:淋巴管生成是淋巴结转移的一个重要因素;PDGF-BB参与了淋巴管生成,但对于促进淋巴结转移可能不是必要的;VEGF-C通过参与淋巴管生成而促进淋巴结转移,其预测NSCLC发生淋巴结转移可能性的价值优于PDGF-BB;PDGF-BB和VEGF-C在促进淋巴管生成的作用上可能具有相关性-

    • 吡那地尔对人肺动脉平滑肌细胞KATP通道mRNA表达的影响

      2008, 28(5):585-588. DOI: 10.7655

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      摘要:目的:研究经典KATP开放剂吡那地尔对原代培养人肺动脉平滑肌细胞KATP通道mRNA表达的影响,以探讨KATP通道在肺动脉高压发生发展中的分子生物学机制-方法:分离人3~4级肺小动脉,原代培养人肺动脉平滑肌细胞-分成ET-1组-ET-1+吡那地尔组及ET-1+吡那地尔+格列本脲组-以Trizol法抽提总RNA,逆转录为cDNA,采用Real-time PCR方法检测各组细胞KATP通道SUR2B和Kir亚单位 mRNA表达量-结果:ET-1组KATP通道SUR2B mRNA表达量较对照组明显减少,为对照组的0.09 ± 0.01倍(P < 0.05,n = 3)-加用吡那地尔可拮抗ET-1作用,提高SUR2B mRNA表达量,为ET-1组的10.94 ± 1.13倍,为对照组的0.97 ± 0.03倍(P > 0.05,n = 3)-格列本脲可拮抗吡那地尔作用,减少SUR2B mRNA表达量,为ET-1+吡那地尔组的0.10 ± 0.01倍,为对照组的0.07 ± 0.02倍(P < 0.05,n = 3)-各组细胞KATP通道Kir6.1 mRNA表达量无统计学差异-结论:KATP开放剂吡那地尔可增加原代培养人肺动脉平滑肌细胞KATP通道SUR2B mRNA表达量,这可能是其调节肺动脉平滑肌细胞KATP通道表达和功能的机制-

    • Aquaporin-1 mRNA在人非小细胞肺癌组织中的表达和意义

      2008, 28(5):589-591612. DOI: 10.7655

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      摘要:目的:探讨 Aquaporin-1(AQP1)mRNA在人非小细胞肺癌组织中的表达和意义-方法:以相应患者手术切除的正常肺组织为对照,采用半定量 RT-PCR技术检测人非小细胞肺癌(17例鳞癌和18例腺癌)组织中 AQP1 mRNA的表达水平-结果:人肺腺癌组织中AQP1 mRNA表达水平为0.419 ± 0.135,高于正常肺组织中的表达水平(0.250 ± 0.124),二者之间差异有统计学意义(P < 0.05);人肺鳞癌组织中 AQP1 mRNA表达水平为0.341 ± 0.099,与正常肺组织中 AQP1 mRNA表达水平(0.264 ± 0.114)之间无统计学差异(P > 0.05)-结论:AQP1 mRNA在人肺腺癌组织中表达增加,提示 AQP1可能与人肺腺癌的发生和发展有关系-

    • 苦参碱?γ干扰素对大鼠肺纤维化干预作用

      2008, 28(5):592-596. DOI: 10.7655

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      摘要:目的:探讨苦参碱-γ干扰素(IFN-γ)对博莱霉素诱导的大鼠肺纤维化的干预作用及其可能的机制-方法:SD雄性大鼠60只,分为健康对照组-肺纤维化模型组-苦参碱干预组-IFN-γ干预组,各组分别于7 d-14 d-28 d处死5只,行HE染色及Masson染色半定量判断肺组织肺泡炎及肺纤维化程度-免疫组化测肺组织TGF-β1-CTGF-I型胶原及Ⅲ型胶原的蛋白水平表达-结果:苦参碱干预组-IFN-γ干预组大鼠肺泡炎-肺纤维化程度均较模型组好转-与模型组相比,苦参碱-IFN-γ都能够抑制肺组织中的TGF-β1-CTGF-I型胶原及Ⅲ型胶原的表达(P < 0.05)-结论:苦参碱及IFN-γ对大鼠肺纤维化模型均有一定的疗效,其作用机制可能是通过抑制TGF-β1和CTGF的表达来减少Ⅰ型胶原及Ⅲ型胶原的生成-

    • 氯胺酮对哮喘模型大鼠氧化应激的影响

      2008, 28(5):597-600688. DOI: 10.7655

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      摘要:目的:研究氯胺酮对哮喘大鼠氧化应激反应产物及相关蛋白表达-方法:采用鸡卵蛋白(OVA)辅以百日咳杆菌菌苗和氢氧化铝为佐剂注射致敏大鼠-雾化吸入OVA激发-51只大鼠随机分成对照组(C组)鸡卵蛋白组(A组),氯胺酮雾化吸入组(K组),氯胺酮腹腔注射组(V组)-C组采用PBS替代OVA进行雾化吸入-A组在激发前给予PBS雾化吸入,K1-K2-K3组大鼠在激发前分别给予12.5-25.0及50.0 mg/ml浓度的氯胺酮雾化吸入,V1-V2组在激发前分别给予50 μg/kg和100 μg/kg的氯胺酮腹腔注射-取大鼠血清-肺组织,分别测定样本中抗氧化应激能力及氧化应激致炎症下游产物P38蛋白的激活-结果:氯胺酮处理组抗OH·-O2·-能力所测得值均高于A组,其中K1-K2-V1组与A组比较差异有显著性(P < 0.01),K3-V2组与A组比较差异有显著性(P < 0.05)-氯胺酮处理组SOD活力明显高于A组,其中K1-K2-V1组与A组比较差异有显著性(P < 0.01),K3-V2组与A组比较差异有显著性(P < 0.05),与C组相比,A组的抗氧化应激能力均降低(P < 0.01)-与C组相比,A组p38的激活程度(pp38/p38)增高(P < 0.01)-p38的激活程度在氯胺酮处理组均降低于A组,其中K1-K2-V1组与A组比较(P < 0.01),K3-V2组与A组比较(P < 0.05)-结论:氯胺酮雾化吸入和腹腔注射可抑制卵蛋白所致的哮喘大鼠肺氧化应激反应的增高,并抑制p38蛋白的激活-雾化吸入氯胺酮12.5 mg/ml和腹腔注射50.0 μg/kg已达到治疗效果-

    • P19细胞向心肌细胞分化过程中FHL1基因的表达变化

      2008, 28(5):601-604. DOI: 10.7655

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      摘要:目的:观察FHL1(Four and a half LIM domains protein 1)基因在P19细胞向心肌细胞诱导分化过程中表达水平的变化,探讨FHL1基因与心肌细胞分化之间的关系-方法:P19细胞经1%二甲基亚砜(DMSO)诱导悬浮培养4天形成细胞聚集体,将聚集体用生长培养基黏附培养至14天,观察细胞跳动情况,用肌钙蛋白I(cTnI)抗体行Western Blot以鉴定心肌细胞分化,并采用RT-PCR-Western Blot技术在细胞分化成熟的不同时段检测P19细胞中FHL1基因mRNA和蛋白的表达水平-结果:DMSO诱导4天后,用生长培养基黏附培养至第8天,P19细胞出现自发性节律跳动的细胞团片,Western Blot检测cTnI蛋白呈阳性-FHL1基因在P19细胞向心肌细胞分化过程中,随细胞分化成熟该基因表达水平逐渐上调,Western Blot检测结果与RT-PCR检测结果基本一致-FHL1基因在分化第0~8天表达显著增高,各时间点之间差异显著(P < 0.05);第8~14天表达趋于稳定,各时间点之间差异无显著性(P > 0.05)-结论:FHL1基因在P19细胞向心肌细胞分化过程中表达逐渐上调,可能参与心肌细胞的分化和发育-

    • CSX/Nkx2.5基因在大鼠胚胎心脏发育过程中的表达

      2008, 28(5):605-608. DOI: 10.7655

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      摘要:目的:检测CSX/Nkx2.5基因在大鼠胚胎心脏发育过程中的mRNA表达变化及其蛋白定位-方法:取大鼠胚胎d12-d15-d17-d19心脏,采用半定量反转录聚合酶链反应(RT-PCR)技术检测心脏组织中CSX/Nkx2.5基因mRNA的表达丰度,并应用免疫组化的方法对其进行定位分析-结果:在大鼠胚胎d12-d15-d17-d19心脏中,CSX/Nkx2.5 基因mRNA均有表达,且呈逐渐上调趋势;其蛋白主要表达于心房-心室-肌小梁等处心肌组织,而在心内膜-心外膜-瓣膜-流出道-大血管等处未见表达-结论:CSX/Nkx2.5 基因的表达随大鼠胚胎心脏的生长发育逐渐上调,且特异表达于胚胎心脏心肌细胞的细胞核,与心脏发育密切相关-

    • 山羊心房颤动模型复律后心房肌细胞凋亡水平的改变

      2008, 28(5):609-612. DOI: 10.7655

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      摘要:目的:研究心房颤动(房颤)复律后心房肌细胞凋亡水平的变化,以探讨房颤复律后心房组织学重构逆转与心房肌细胞凋亡关系-方法:18只山羊随机分为3组(每组6只):窦性心律组(A组);慢性房颤组(B组);房颤后恢复窦性心律组(C组)-三组均行开胸手术,左心耳缝入起搏电极,A组不起搏,3个月后处死;B-C组持续快速起搏(400 ± 10次/min)制作慢性房颤模型,B组起搏3个月后处死;C组起搏3个月后停止起搏,用心律平静脉注射或直流电复律,维持窦律3个月后处死-取左心房组织,用TUNEL法标记并用流式细胞仪检测心房肌细胞凋亡-结果:慢性房颤组左房心肌细胞凋亡比窦性心律组显著增高(P < 0.05),房颤后复律组左房心肌细胞凋亡仍高于窦性心律组,但比慢性房颤组有所下降(P < 0.05)-结论:房颤复律前后心房肌细胞凋亡水平随心房组织学重构的进展而升高,随重构的逆转而下降-

    • Akt参与热休克蛋白27保护大鼠心肌细胞过氧化损伤

      2008, 28(5):613-617. DOI: 10.7655

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      摘要:目的:探讨小分子热休克蛋白27保护大鼠心肌细胞过氧化损伤的机制-方法:①以稳定转染pCDNA3.1/Hsp27质粒并稳定高表达Hsp27的大鼠心肌细胞株H9c2(H9c2-Hsp27)为研究对象,对照组采用稳定转染pCDNA3.1质粒的H9c2(H9c2-Vector)细胞;②氧化应激诱导和检测:500 -滋mol/L H2O2处理细胞后进行如下分析:培养上清中的LDH活性-细胞形态学改变-细胞内Akt激活水平;③Akt在Hsp27保护H2O2诱导H9c2 损伤中的作用:以Akt抑制剂Triciribine处理H9c2-Hsp27,观察其对Hsp27保护H2O2诱导H9c2 形态学变化的影响-结果:①H2O2诱导H9c2细胞向培养上清释放的LDH显著增加(P < 0.01),但与对照组相比,Hsp27高表达显著抑制了H2O2诱导的LDH释放(P < 0.01);②H2O2处理后,H9c2细胞Akt磷酸化水平增加(P < 0.01或P < 0.05),但与H9c2-Vector比较,H9c2-Hsp27的Akt磷酸化水平进一步增强(P < 0.05);③Akt磷酸化被抑制后,Hsp27对H2O2 诱导H9c2细胞形态学改变的保护作用消失-结论:Akt激活是Hsp27保护大鼠心肌细胞过氧化损伤的重要机制-

    • 阿替洛尔对过氧化氢损伤乳鼠心肌细胞有保护作用

      2008, 28(5):618-620634. DOI: 10.7655

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      摘要:目的:观察阿替洛尔对过氧化氢(H2O2)致心肌细胞氧化损伤的保护作用,并探讨此作用的机制-方法:用H2O2作用于新生SD大鼠心肌细胞,建立心肌细胞过氧化氢损伤模型-用阿替洛尔预处理后,观察心肌细胞状态,测定心肌细胞存活率-乳酸脱氢酶(LDH)-丙二醛(MDA)活性-结果:阿替洛尔提高心肌细胞过氧化损伤的细胞存活率(P < 0.05),减少LDH和MDA的生成(P < 0.05)-结论:阿替洛尔对心肌细胞过氧化损伤有一定起保护作用-

    • 缬沙坦对心肌梗死大鼠心肌胶原重塑?ASK1的影响

      2008, 28(5):621-624. DOI: 10.7655

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      摘要:目的:探讨心肌梗死模型下血管紧张素Ⅱ(AngⅡ)受体拮抗剂缬沙坦对凋亡信号调节激酶1(ASK1)介导心室重塑信号通路的影响-方法:复制心肌梗死大鼠模型,苦味酸天狼猩红染色法检测心肌胶原容积分数,免疫印迹法检测心肌ASK1蛋白含量并测定大鼠血流动力学和左心室质量指数-结果:缬沙坦组左心室质量指数-胶原容积分数-ASK1表达均显著低于心肌梗死组(P < 0.05)-结论:ASK1的激活参与心肌梗死后心室重塑过程,缬沙坦能干预ASK1介导通路并改善心肌胶原重塑-

    • Fas(TNFRSF6)基因位点A670G多态性与子痫前期的相关性研究

      2008, 28(5):625-628. DOI: 10.7655

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      摘要:目的:探讨Fas(TNFRSF6)基因位点A670G多态性与子痫前期(pre-eclampsia)的相关性-方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对140例子痫前期患者(轻度子痫前期75例,重度子痫前期65例)和170例同期入院产检并在南京医科大学第一附属医院分娩的正常孕妇,进行Fas(TNFRSF6)基因位点A670G多态性的分析,比较各基因型及等位基因的频率在病例组和正常组间-轻度子痫前期组和重度子痫前期组间的差别-结果:G等位基因型频率在子痫前期组(56.4%)显著高于对照组(36.2%)(P < 0.001),G等位基因型频率在重度子痫前期组(64.6%)显著高于轻度子痫前期组(49.3%)(P = 0.010)-结论:Fas(TNFRSF6)基因位点A670G多态性的G等位基因可能是子痫前期的重要遗传危险因素,并与病情的严重程度相关-

    • IL-1B及IL-1RN基因多态性与江苏地区宫颈癌遗传易感性相关

      2008, 28(5):629-634. DOI: 10.7655

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      摘要:目的:探讨炎症因子IL-1B(T-31C和C-511T)及其拮抗基因IL-1RN基因多态性与宫颈癌遗传易感性的关系-方法:采用病例对照研究,对经组织学确诊的宫颈癌新发病例273例按年龄和城乡频数匹配正常对照402例,以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法进行多态性检测,比较不同基因型与宫颈癌发病风险的关系-结果:在调整年龄-城乡等因素后,携带-511CT基因型比携带CC基因型个体发生宫颈癌的危险性增加93%(调整OR=1.93,95%CI=1.00~3.72);携带-511TT基因型比携带-511CC基因型个体发生宫颈癌的危险性增加115%(调整OR=2.15,95%CI=1.02~4.53)-分层分析表明,携带IL-1B T-31C变异基因型的个体发生宫颈癌的风险仅在年龄≥50岁(调整OR=1.72,95%CI=1.01~2.93)-妊娠次数≥2次(调整OR=1.83,95%CI=1.07~3.11)和农村地区女性(调整OR=1.63,95%CI=1.04~2.54)中显著-IL-1RN各基因型在病例组和对照组中分布的差异均无统计学意义-结论:IL-1B C-511T多态性可能与江苏地区妇女的宫颈癌易感性相关-

    • TNF-α及sTNFRⅠ对子宫内膜异位症在位内膜基质细胞的作用

      2008, 28(5):635-639. DOI: 10.7655

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      摘要:目的:探讨肿瘤坏死因子α(tumor necrosis factor,TNF-α) 和可溶性肿瘤坏死因子受体(soluble TNFR I,sTNFR I)对体外培养的子宫内膜异位症(Endometriosis)患者在位内膜基质细胞的影响,为子宫内膜异位症的生物学治疗提供新思路-方法:对EMS 患者的在位子宫内膜基质细胞进行体外培养,分别用不同浓度的TNF-α(0-0.1-1.0-10.0和100.0 ng/ml)或者同一浓度TNF-α(1 ng/ml)培养不同时间(4-8-12-24-48和72 h)或者TNF-α(1 ng/ml)和sTNFRⅠ(2 -滋g/ml)对基质细胞进行刺激并收集细胞上清液,利用双抗体夹心ELISA 检测细胞培养上清液中IL-6-基质金属蛋白酶-3(matrix metalloproteinases,MMP-3)的水平-结果:TNF-α可促进体外培养的EMS 子宫内膜基质细胞IL-6-MMP-3的分泌,而且IL-6 -MMP-3浓度与TNF-α之间存在剂量-时间依赖关系(P < 0.05),而sTNFRⅠ具有抑制TNF-α的作用-结论:TNF-α在EMS的发病机制中发挥重要的作用,其对EMS 患者子宫内膜基质细胞的促黏附-侵袭-增殖等作用可能是通过促进IL-6-MMP-3 的分泌来完成的;sTNFRⅠ有望用于EMS生物学治疗-

    • 神经营养因子相关基因在骨髓基质细胞分化为神经元中的表达

      2008, 28(5):640-645. DOI: 10.7655

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      摘要:目的:体外诱导骨髓基质细胞分化为神经细胞,并观察诱导分化前后神经营养因子及其受体相关基因的表达变化,以探索骨髓基质细胞诱导分化为神经细胞的可能机制-方法:体外培养经Percoll(1.073 g/L)分离获得的大鼠骨髓基质细胞并传代扩增,用二甲亚砜(DMSO),丁羟回醚(BHA)诱导分化,采用倒置相差显微镜观察细胞形态学变化,分化后细胞用神经丝蛋白(NF)抗体,胶质源性纤维酸性蛋白(GFAP)抗体作细胞免疫荧光检测-采用半定量RT-PCR检测分化前和分化后2 h,6 h,12 h,24 h和48 h神经生长因子(NGF),脑源性神经因子(BDNF),神经营养因子-3(NT-3)及其相应受体TrkA,TrkB,TrkC和神经营养因子低亲和力受体p75NTR的表达变化-结果:①骨髓基质细胞体外诱导分化后2 h即出现形态学变化,表现为细胞胞体呈球形,突起增多,形态类似神经元-细胞免疫荧光检测显示神经丝蛋白(NF)抗体标记的神经元细胞阳性,GFAP阴性-②在骨髓基质细胞分化前后都有NGF和BDNF持续表达,但分化后NGF表达降低,TrkA,TrkB在分化前细胞中不表达,在诱导分化开始后12 h可以检测到他们的表达,而NT-3,TrkC在分化前后都不表达-③p75NTR在诱导分化后6 h开始出现表达,12 h后显著降低并很快消失-结论:骨髓基质细胞在体外可以定向诱导为神经细胞,诱导分化前后神经营养因子(NT)及其Trk受体和p75受体的表达发生了显著变化,NT与Trk受体及p75受体作用的信号途径可能在骨髓基质细胞分化为神经细胞过程中起重要作用-

    • 辅酶Q10能有效抑制鱼藤酮诱导的细胞毒性作用

      2008, 28(5):646-649. DOI: 10.7655

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      摘要:目的:探讨辅酶Q10对鱼藤酮介导的多巴胺能神经元损伤是否具有保护作用并研究其机制-方法:原代培养孕14 d的胎鼠中脑多巴胺能神经元,以不同剂量的鱼藤酮建立细胞凋亡模型-通过TH免疫细胞化学进行鉴定,测定LDH值以了解细胞活力,以罗丹明123染料进行流式细胞仪检测线粒体膜电位(△Ψm)-结果:鱼藤酮干预多巴胺能神经元24 h后,细胞活力及△Ψm均明显下降,与0 nmol/L鱼藤酮组比较,15 nmol/L及30 nmol/L鱼藤酮组的LDH值(U/ml)显著增加(P < 0.05),其△Ψm(%)则明显下降(P < 0.05);辅酶Q10 12 h能显著缓解细胞△Ψm的下降,与15 nmol/L鱼藤酮组比较,50 μmol/L及100 μmol/L辅酶Q10预处理组的△Ψm(%)明显上升(P < 0.05)-结论:辅酶Q10能有效抑制鱼藤酮诱导的细胞毒性作用-

    • 单?多循环缺血预处理对缺血性肾损伤保护作用的研究

      2008, 28(5):650-653. DOI: 10.7655

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      摘要:目的:探讨单-多循环两种缺血预处理方案对大鼠肾脏急性缺血再灌注损伤的保护作用-方法:40只雄性SD大鼠经切除右肾-分离出左肾动脉后随机均分为4组:①缺血再灌注(I/R)组:直接夹闭左肾动脉45 min恢复血供24 h;②单循环缺血预处理(SCP)组:夹闭左肾动脉10 min,开放血流10 min,仅一个循环,余同I/R组;③多循环缺血预处理(MCP)组:夹闭左肾动脉2 min,开放血流5 min,反复三个循环,余同I/R组;④假手术(Sham)组:暴露术野60 min后,直接缝合-24 h后取材行生化-病理检查及肾小管损伤评分-结果:sham组未见明显改变;I/R-SCP-MCP组血肌酐(Scr)水平及肾小管损伤评分明显高于sham组(P < 0.01),MCP组低于I/R组,差异显著(P < 0.01);SCP组低于I/R组,差异显著(P < 0.05);MCP组低于SCP组,差异显著(P < 0.01)-结论:缺血预处理可从功能和组织学上减轻肾脏的急性缺血再灌注损伤,多循环预处理方案的保护作用在24 h内强于单循环的方案-

    • 低锌对未成熟大鼠视网膜锌离子转运体3(ZnT-3)mRNA表达的影响

      2008, 28(5):654-657. DOI: 10.7655

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      摘要:目的:通过观察视网膜锌离子转运体3(ZnT-3)mRNA表达对低锌饮食的反应,探索低锌是否会引起ZnT-3转录水平的调节-方法:将36只雄性SD大鼠(4周龄)随机分为三大组分别喂养2周(第1组)-4周(第2组)-6周(第3组),每组大鼠又随机分为两个亚组分别给予正常锌含量饮食和低锌饮食,第3组低锌饮食大鼠从第4周开始恢复正常饮食-用逆转录-聚合酶链反应(RT-PCR)法检测ZnT-3以及碳酸酐酶2(CA2)-碳酸酐酶14(CA14)的mRNA相对表达量-结果:大鼠锌摄入低下2周后,与空白对照组相比视网膜CA2和CA14的mRNA水平有所降低,而ZnT-3 mRNA表达量有所增高;锌摄入低下4周后,视网膜ZnT-3-CA2和CA14 mRNA表达量均明显下降;恢复正常锌饮食后2周,实验组视网膜ZnT-3-CA2和CA14的mRNA的表达回升与对照组无差异-结论:饮食锌的变化将导致大鼠视网膜ZnT-3在转录水平上的调节,含锌酶CA2和CA14mRNA表达也同时发生变化-为保持视网膜组织内锌含量的稳定,ZnT-3mRNA表达量随饮食含锌量降低的时间和程度而改变-

    • 膜联蛋白A10在人食管鳞癌及其癌旁组织中的表达及临床意义

      2008, 28(5):658-661. DOI: 10.7655

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      摘要:目的:探讨膜联蛋白A10(annexin A10)在人食管鳞癌和癌旁组织中的表达以及临床意义-方法:应用半定量RT-PCR-定量PCR-Western blot法,检测40对配对人食管鳞癌和癌旁组织中annexin A10表达差异;并分析该表达差异与临床病理特征如性别-年龄-肿瘤大小-浸润深度-临床分期-淋巴结转移和细胞分化的关系-结果:40对配对人食管鳞癌组织中,annexin A10 mRNA表达水平癌组织77.06 ± 37.11,癌旁组织66.99 ± 17.62;蛋白表达水平癌组织为1.73 ± 1.30,癌旁组织1.26 ± 1.18,癌组织和配对癌旁组织表达差异有统计学意义(P < 0.05)-annexin A10在癌组织和癌旁组织的表达差异在淋巴结转移组和未转移组,在高中低分化组均有显著性差异,而与性别-年龄-肿瘤大小-浸润深度-临床分期关系无统计学意义-结论:annexin A10在人食管鳞癌中表达上调,并随肿瘤分化程度降低-淋巴结转移而表达增高-annexin A10可能成为食管鳞癌诊断和预后的一个新的指示靶点-

    • 溃疡性结肠炎患者P-选择素检测和血小板计数的临床意义

      2008, 28(5):662-664. DOI: 10.7655

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      摘要:目的:探讨溃疡性结肠炎(ulcerative colitis,UC)和血小板计数及血小板活化状态的关系-方法:对54例活动期溃疡性结肠炎-26例缓解期溃疡性结肠炎和正常对照组30例采用血细胞记数仪测定血小板数量,流式细胞术检测P-选择素(CD62p)-结果:①血小板计数:活动期UC患者血小板计数[(212.2 ± 97.6) × l09/L]明显高于缓解期[(168.7 ± 67.8) × l09/L]和对照组[(166.8 ± 81.4) × l09/L](P < 0.01);缓解期患者与对照组相比无明显差异(P > 0.05)-②P-选择素水平:与对照组(3.9 ± 1.2%)和缓解期(8.6 ± 2.1%)患者组比较,活动期溃疡性结肠炎患者P-选择素(13.3 ± 3.4)%显著升高(P < 0.01),缓解期患者P-选择素仍高于正常对照组(P < 0.05)-③在活动期UC患者中,对于不同的病情程度,血小板计数与P-选择素水平有显著性差异-结论:活动期溃疡性结肠炎体内存在血小板活化;P-选择素和血小板计数的变化对评价病情活动性与严重程度均有良好的价值-

    • M2A胶囊内镜在45例小肠疾病诊断中的应用再探

      2008, 28(5):665-666. DOI: 10.7655

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      摘要:目的:对M2A胶囊内镜在小肠疾病中的诊断价值进行再评估-方法:对经胃镜-全结肠镜-全消化道钡餐检查-腹部血管造影等检查无阳性发现的45例病变疑在小肠的患者,在进行适当的肠道准备后应用胶囊内镜进行小肠检查,再评估胶囊内镜检查在小肠疾病诊断中的应用价值-结果:45例中发现小肠病变39例(86.67%),包括小肠血管畸形19例-小肠非特异性炎性病变5例-增生性病变2例-Crohn病3例-小肠钩虫病1例-小肠息肉5例-末端回肠炎3例,6例未见异常发现-检查过程中患者无任何不适,也无并发症发生,所获取的图像质量良好-结论:胶囊内镜能清晰安全卫生地观察小肠,对小肠疾病的诊断具有较高应用价值,可作为小肠疾病检查的首选方法-

    • 检测血浆sCD74有助于肺癌诊断

      2008, 28(5):667-669. DOI: 10.7655

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      摘要:目的:探讨检测血浆游离CD74的浓度在鉴别肺癌和肺部良性疾病中的意义-方法:取肺癌患者和慢性阻塞性肺病患者的外周血5 ml,乙二胺四乙酸(ethylenediamine tetraacetic acid,EDTA)抗凝,离心取血浆-70℃保存-用双抗体夹心酶联免疫吸附测定法测定血浆中的sCD74的浓度-结果:按照病理类型实验组分为腺癌组-鳞癌组;对照组为慢性阻塞性肺病患者-腺癌组-鳞癌组-对照组的sCD7水平分别为(2.51 ± 1.36)ng/ml-(2.14 ± 1.16)ng/ml和(1.91 ± 0.78)ng/ml,腺癌患者血浆sCD74含量高于鳞癌患者和对照组-腺癌患者和鳞癌患者相比,无统计学差异(P = 0.293 > 0.05);腺癌患者与对照组相比,有统计学差异(P = 0.037 < 0.05);鳞癌患者和对照组相比,无统计学差异(P = 0.893 > 0.05)-结论:检测血浆sCD74可能有助于肺癌的诊断-

    • 多发伤患者早期N末端脑利钠肽前体动态变化及其伤情评估价值

      2008, 28(5):670-672. DOI: 10.7655

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      摘要:目的:探讨多发伤患者早期血浆N末端脑利钠肽前体(NT-proBNP)的动态变化及其与合并心肌损害的关系,同时评价其伤情评估价值-方法:收集2005年8月~2007年4月间多发伤住院患者15例,按照APACHEⅡ评分将患者分为重伤组(≥15分)7例与一般伤害组(<15分)8例-分别于入院后第1-3-5-7天进行NT-proBNP与cTnI动态检测-结果:多发伤患者第1天血浆NT-proBNP与APACHEⅡ评分呈正相关(r = 0. 601,P < 0.001)-两组患者第1-3-5-7天NT-proBNP及cTnI比较均具有统计学意义,其中重创组升高更为显著(组间比较,P < 0.01)-结论:多发伤患者NT-proBNP动态变化可能与心肌损伤有关,NT-proBNP可以用来进行多发伤患者早期伤情评估-

    • 经口咽入路治疗颅颈区腹侧畸形的解剖及临床研究

      2008, 28(5):673-676. DOI: 10.7655

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      摘要:目的:探讨经口咽入路显微手术治疗颅颈交界区腹侧畸形的要点,并对相应的显微解剖学基础加以讨论-方法:选用福尔马林固定的成人尸头标本15例进行解剖学观察;并应用该入路治疗颅颈交界区腹侧畸形16例,对其临床资料进行回顾性总结-结果:经口咽入路手术可以达到斜坡下部至颈3椎体上缘,双侧椎动脉到中线的距离C1,2水平约20.1 mm,C2,3水平约16.2 mm;16例患者中痊愈11例,好转4例,无效1例,手术并发症包括1例脑脊液漏,1例严重的舌肿胀,无死亡病例- 结论:经口咽入路显微手术具有手术径路短,创伤小-并发症少的优点,是治疗颅颈交界区腹侧畸形的首选方法-

    • 腘血管压迫综合征28例的诊断和外科处理

      2008, 28(5):677-678. DOI: 10.7655

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      摘要:目的:探讨腘血管压迫综合征的临床特点及治疗方法-方法:对1992~2005年诊治的28例腘血管压迫综合症患者进行回顾分析-术前通过临床表现-彩色超声多普勒-数字减影血管造影(DSA)-磁共振血管造影(MRA)-计算机断层扫描血管成像(CTA)等检查明确定性及定位诊断-结果:28例均行手术治疗,结果证实为腘血管压迫综合征-结论:腘血管压迫综合征强调早期诊断-早期治疗-治疗以解除异常压迫-重建血运为主-外科手术是治疗该病的唯一方法-

    • 经食管超声心动图在小儿微创房间隔缺损封堵术中的应用

      2008, 28(5):679-680. DOI: 10.7655

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      摘要:目的:回顾性分析经食管超声心动图(TEE)在小儿微创房间隔缺损(ASD)封堵术中的应用-方法:本组22例,年龄1~10岁,术前TEE检查均为继发孔中央型房间隔缺损,于手术开始前,在全身麻醉下插入经食管探头,再次明确ASD大小,残缘长度,选择合适的封堵器-术中在TEE严密监测引导下,行右前胸第四肋间胸骨旁1.5~2.0 cm微创小切口经胸膜外房间隔缺损封堵术,并即刻评价封堵效果,确认是否有残余分流或并发症-结果:22例手术均成功完成封堵,TEE显示封堵器与ASD边缘吻合紧密,无残余分流及并发症-结论:TEE对选择适合封堵术的ASD患者,选择封堵器的大小,指导封堵器的释放,以及疗效评价均具有重要作用-

    • 肝脏局灶性结节增生的超声造影诊断

      2008, 28(5):681-684. DOI: 10.7655

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      摘要:目的:探讨肝脏局灶性结节增生(FNH)在超声造影中的特征性表现及与病灶大小的关系-方法:经病理学检查证实的FNH 26例,采用美国GE公司LOGIQ9及意大利百胜公司DU8彩色多普勒系统,使用意大利Bracco公司造影剂Sonovue进行超声造影成像,造影时记录肝实质及病灶的充填过程-充盈方式及增强程度-结果:在直径大于3.0 cm的15例病灶中,14例(93.3%)在动脉相出现“轮辐状”增强征象-13例(86.6%)在延迟相病灶中央或偏心出现瘢痕的征象-结论:对于大于3.0 cm的病灶可以根据超声造影中特有的辐轮征和中央瘢痕来作为FNH的诊断依据-

    • 多层螺旋CT颈?脑血管成像48例临床应用价值

      2008, 28(5):685-686692. DOI: 10.7655

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      摘要:

    • 脑膜血管型神经梅毒2例误诊分析

      2008, 28(5):687-688. DOI: 10.7655

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      摘要:目的:探讨脑膜血管型神经梅毒的临床表现及误诊原因-方法:对2例脑膜血管型神经梅毒病例进行回顾性分析-结果:2例均表现智能障碍,脑室伴管腔梗死,其临床表现与多种神经疾病有相似之处,极易误诊-2例患者均长期未明确诊断并被误诊,最终经查梅毒螺旋体特异性抗体组套(血清TPPA检测阳性)明确诊断-结论:掌握脑膜血管型神经梅毒的临床特点,规范化的诊疗程序及详尽的病史采集是减少误诊的有效措施-

    • 江苏地区乳腺癌危险因素的病例对照研究

      2008, 28(5):689-692. DOI: 10.7655

      摘要 (2238) HTML (57) PDF 0.00 Byte (116) 评论 (0) 收藏

      摘要:目的:调查江苏地区女性乳腺癌危险因素,为确定高危人群-提出预防措施提供依据-方法:采用以医院为基础的病例对照研究,对江苏地区经病理证实的515例乳腺癌患者,按照年龄和地区匹配,选取对照515例进行统一的问卷调查,用SPSS13.0软件进行单因素和多因素logistic分析-结果:在单因素分析中,达到显著性水平的危险因素为首次活产年龄大-有流产史-初潮年龄小-有肿瘤家族史等,达到显著性水平的保护因素为母乳喂养及口服避孕药-非条件logistic多元回归分析显示,有统计学意义的乳腺癌危险因素是肿瘤家族史-有流产史,重要保护性因素是母乳喂养-结论:江苏地区女性乳腺癌发病不仅与月经生育史有关,还与肿瘤家族史有一定联系-