摘要:Spinal cord injury(SCI) is a severe trauma to the central nervous system(CNS). This article reviews recent advances in cellular transplantation to treat SCI. Transplanted cells can supply new neurons to replace injured ones, promote regeneration of axons and myelin sheath, modulate the inflammatory response, and thus promote recovery from traumatic injury of the CNS. Cellular transplantation is a promising potential method for the treatment of SCI.

摘要:Tako-tsubo cardiomyopathy(TC) is a recently described acute cardiac syndrome, which the latest cardiomyopathy classification of the European Society of Cardiology describes as an unclassified cardiomyopathy. TC mimics acute myocardial infarction(AMI) and is characterised by ischaemic chest symptoms, an elevated electrocardiogram ST-segment, and moderately increased levels of cardiac disease markers. However, patients with TC have no coronary angiogram-detectable or non-obstructive coronary arterial disease(CAD), and left ventriculography documents transient left apical and middle ventricular wall dysfunction. In this review, we describe TC and evaluate epidemiological, clinical and instrumental features, pathophysiological mechanisms, therapy and prognosis of this syndrome，with a view to raising awareness of the disease.

摘要:Objective: To investigate the expression of herg1 gene in tumor tissues from gastric carcinomas and gastric carcinoma cell lines, and study the relationship between HERG K+ channel expressions and tumor cell proliferation and apoptosis. Methods: RT-PCR and PCR assays were used to detect the expression of herg1 gene in 64 gastric carcinomas and the gastric cancer cell line SGC-7901. Blocking the HERG K+ channels was used to evaluate their effects on tumor cell proliferation and apoptosis. Results:The statistically significant expression of herg1 gene was detected in all the gastric cancers and SGC-7901 cells, but not in normal tissues. The HERG K+ channel blocker, E-4031, increased the cell population in G0/G1(P < 0.05) and the number of apoptotic tumor cells(P < 0.05). Conclusion: HERG K+ channels were expressed in all gastric carcinomas tested and these channels appear to modulate tumor cell proliferation and apoptosis.

摘要:Objective:Myocardial ischemia/reperfusion(I/R) injury is the leading cause of death in the world. However, the details of the mecha-nism of its pathophysiology are still unknown. The present study was designed to investigate the role of connexin 43(Cx43) in acute models of myocardial I/R injury. Methods: Male C57BL/6 mice were subjected to myocardial ischemia(45 min) followed by reperfusion(4 hrs) in vivo. The whole operation was monitored using a two-lead ECG. Hearts were harvested and the level of protein was assessed by western blot analysis. Haematoxylin and Eosin(HE) staining was used to detect the extent of neutrophil infiltration. The expression level of IL-6 was detected by ELISA. Results: A murine myocardial I/R injury model was constructed successfully. Phosphorylated Cx43 decreased 83.45% while non-phosphorylated Cx43 increased 1.62- fold in the myocardium after I/R injury. Neutrophil infiltration and the expression of the inflammatory cytokine IL-6 increased in the myocardium following I/R. Conclusion: During myocardial I /R injury, cardiomyocyte Cx43 is dephosphorylated, and this may be associated with an inflammatory response.

摘要:Objective:To explore a new natural antibiotic. Methods:The chemical composition of the essential oil from Ganoderma japonicum (G. japonicum) mycelia was analyzed by gas chromatography-mass spectrometry(GC-MS). The antimicrobial activity of the oil was evaluated against eighteen microorganisms, including bacteria, mildew and yeast by using a disc diffusion method. Furthermore, the minimum inhibitory concentrations(MIC) and the minimum bactericidal concentrations(MBC) of the essential oil against twelve clinical pathogens were determined. Results:The main components of the oil were nerolidol, decadienal, linalool and benzyl alcohol. The antimicrobial results indicated that the oil inhibited all the tested bacterium, especially Methicillin-resistant Staphylococcus aureus (MRSA) in which the antibacterial activity exhibited a MBC of 1.03 mg/ml. Conclusion: The essential oil of G. japonicum mycelium has significant inhibitory activity. It is a potential medicinal resource that can be used as a natural antibiotic.

摘要:Objective:The purpose of this study was to evaluate the effects of Enterococcus faecalis FK-23(FK-23) on the immune function in mice. Methods:Mice were treated with three doses of the FK-23(0.05, 0.1, 0.2, 0.6 g/kg body weight) for 30 days. Delayed type hypersensitivity (DTH), monocyte phagocytic function, serum hemolysis antibody and natural killer cell activity were studied. Results:The significant increases in mice treated with 0.6 g/kg bw were found in DTH, phagocytic function of monocyte, serum hemolysis antibody and natural killer cell activity. Conclusion:The results from the present study suggest that FK-23 could improve both specific and non-specific immune function.

摘要:Objective: To observe the efficacy and safety of topical imiquimod 5% cream in the treatment of uncomplicated infantile hemangiomas(IHs). Methods: A total of 68 IHs were treated with topical imiquimod 5% cream. Among them, 36 were superficial, 22 were mixed, and 10 were deep. The size of IHs ranged from 1.0 cm×1.5 cm to an area of a whole forearm. All the hemangiomas were in a proliferative stage. Imiquimod was applied 3 times weekly in 44 patients and 5 times weekly in 24 patients for up to 36 weeks. Results: All superficial IHs improved, and 18 achieved complete clinical resolution, 10 had excellent improvement, 5 showed moderate improvements, and 3 patients displayed minimal improvement. Two mixed IHs showed excellent improvement, 3 showed moderate improvement and 5 mani-fested minimal improvements. The remaining 12 mixed IHs and all deep IHs did not respond to the therapy. The total incidence of local adverse events was 58.82%(40/68), which included erythema or edema, local itching, incrustation or peeling, erosion or ulceration, although most of these were mild to moderate reactions and did not affect the treatment. Scarring occurred in 2 mixed IHs. No systemic side effects developed. Conclusion: Imiquimod 5% cream may be a safe and effective alternative for the treatment of superficial IHs and some mixed IHs in which the superficial component predominates. An appropriate treatment duration for proliferative IHs treated with this therapy may be 24 weeks. Some local adverse events, such as crusting and erosion with possible scarring potential may occur and should be addressed by prompt, but temporary, discontinuation of the imiquimod. Topical imiquimod 5% cream can be prudently used in the treatment of IHs larger than 5.0 cm×5.0 cm in newborns and infants less than 6 months of age. To our knowledge, this is the largest IH group treated with imiquimod that has been reported in the literature to date.

摘要:近年来,在中药注射剂得到广泛应用的同时,其所引发的不良后果事件逐渐增多,中药注射剂的安全问题引起了人们的关注-文章首先对中药注射剂的安全现状进行了描述,分析了中药注射剂所引发的不良后果,并重点从生产环节-流通存储环节-使用环节-监管环节入手分析了影响中药注射剂安全的因素,最后从管理的视角提出了中药注射剂的安全策略-

摘要:农民工张海超为确诊个人患有尘肺病不惜冒生命危险“开胸验肺”,这一事件引发了社会对农民工职业病维权问题的关注-我国农民工职业病维权面临着工伤认定程序漫长和维权成本高等困境-究其原因主要是法律不健全,职业病鉴定机构监管的缺失以及社会保障缺失-若要化解农民工职业病维权困境,必须健全相关法律,完善工伤保险制度并加强政府主导作用,只有这样才可切实保障农民工职业病患者权益-

摘要:Objective:To investigate the efficacy and safety of the second generation argon plasma coagulation(VIO APC) in the ablation of Barrett’s Esophagus. Methods:A total of 35 patients with uncomplicated Barrett’s esophagus entered into a prospective, randomized, unblinded study comparing the treatment VIO APC combined with a proton pump inhibiter with a proton pump inhibiter administered alone. VIO APC was performed at a power setting of 40W, and argon gas flow at 1.5-2.0 L/min, and“forced”mode. Ablative treatment was repeated until either no Barrett’s epithelium remained or a maximum of 5 treatment sessions occurred. Results:In the ablation group, macroscopic complete ablation was achieved in 14 of 18 patients, and complete ablation confirmed by histology in 12 of 18 patients(P < 0.01). Buried glands were observed in 2 patients who had achieved macroscopic ablation. The Barrett’s mucosa averaged a reduction of 65%(range 50-75%) in the remaining 4 patients. In the control group, only 2 patients had partial regression, median 30%(range 20-40%). In the ablation group, post-treatment 4 patients had transient retrosternal pain, and 3 patients had mild epigastric discomfort. One patient had a small hemorrhage during the procedure, which ceased after norepinephrine and thrombosin were administered through the endoscope biopsy channel. No adverse events were observed in the control group. During 11.8(4-15) months follow-up, patients who had achieved the complete ablation have no evidence of relapse of Barrett’s esophagus. Conclusion:VIO APC with a relatively low power setting can effectively ablate the Barrett’s mucosa. No severe adverse events were observed. Long-term follow-up is needed to assess cancer prevention and the durability of the neo-squamous epithelium.

摘要:文章重点阐述了突发公共卫生事件的概念及特点,分析了突发公共卫生事件期间社会公众疑病-恐慌-焦虑-抑郁-强迫-盲从等社会心理特点,最后提出构建突发公共卫生事件的社会心理支持体系,包括建立良好的风险沟通机制-发挥主流媒体功能-建立应急处理机制-改善认知模式-增进人际交往-

摘要:Objective: To learn the status of tobacco use, and the knowledge, attitudes, and behaviors among female students in Bengbu Medical College. Methods: In a cross-sectional survey, questionnaires were completed by 634 female students in the medical college in 2007, including the prevalence of current smoking, their knowledge of the effects of tobacco use on health, and attitudes towards the smoking behaviors of young women. Results: Only 6.9% of female medical students were former smokers, and 4.9% of them were current smokers. There was no significant difference in the current smoking rate among the students from each department surveyed. Female students from urban areas were more likely to be current or attempted smokers than those from rural areas. The proportion of the students who were aware of the health risks of smoking was less than 45%. The students from the Department of Nursing had more knowledge regarding the harmful health effects of smoking than those from the other departments. There was no significant difference in attitudes towards the smoking behaviors of young women among the students from each department. Compared with female students from rural areas, the female students from urban areas were significantly more likely to think that a young woman who smoked was cool, mature and charming. Conclusion: The smoking prevalence of the female students in Bengbu Medical College is high. They are not aware of the smoking related risks and have erroneous beliefs and perceptions about female smoking behaviors.

摘要:食品安全问题源自于政府的多头监管和市场信息的不对称-部分生产经营者的主观故意以及我国目前食品检测技术的落后和消费者防范意识的薄弱-在解决食品安全问题方面,发达国家和地区的做法值得借鉴-当前,必须整合食品安全管理机构,建立全方位食品安全监控机制,完善食品安全法律体系,加强对民众的食品安全观教育,培育食品安全监管的社会力量-

摘要:Objective:To identify and isolate CD133 positive cancer stem-like cells (CD133+ cells) from the highly invasive human hepatocellular carcinoma cell line(MHCC97H), and examine their potential for clonogenicity and tumorigenicity. Methods: CD133+ and CD133- cells were isolated from MHCC97H cell line by magnetic bead cell sorting(MACS), and the potentials of CD133+ cells for colony formation and tumorigenicity were evaluated by soft agar cloning and tumor formation following nude mice inoculation. Results:CD133+ cells represent a minority(0.5-2.0%) of the tumor cell population with a greater colony-forming efficiency and greater tumor production ability. The colony-forming efficiency of CD133+ cells in soft agar was significantly higher than CD133- cells(36.8±1.4 vs 12.9±0.8, P ＜0.05). After 6 weeks, 3/5 mice inoculated with 1×103 CD133+ cells, 4/5 with 1×104 CD133+ cells and 5/5 with 1×105 CD133+ cells developed detectable tumors at the injection site, while only one tumor was found in mice treated with same numbers of CD133- cells. Conclusion: CD133 may be a hallmark of liver cancer stem cells (CSC) in human hepatocellular carcinoma(HCC), because the CD133+ cells identified and isolated with anti-CD133 labeled magnetic beads from MHCC97H cell line exhibit high potentials for clonogenicity and tumorigenicity. These CD133+ cells might contribute to hepatocarcinogenesis, as well as the growth and recurrence of human HCC, and therefore may be a useful target for anti-cancer therapy.

摘要:网瘾患者成了精神病人,孩子得了精神病就不得不花重金去治疗,那么戒网瘾用重典,包括封闭式的残酷军训-精神用药甚者电击治疗,一时各种机构-学校-训练中心等如雨前蚂蚁,各显神通-直到国家卫生部叫停电击疗法,这场闹剧才得以收场-文章就该事件从医学角度对《网络成瘾临床诊断标准》进行分析,提出网瘾患者大多为心理问题,对网瘾治疗治病需要先治医-

摘要:毛泽东民族资本主义观的转折点问题是学界关注的焦点,也存在较大分歧-毛泽东民族资本主义观的转变经历了一个过程,“九月会议”是转变的起点,而建国前后对民族资产阶级既利用又限制的政策则是这种转变的进一步发展,最后,在“三反五反”运动中,毛泽东完全改变了对民族资产阶级的认识,转而认为要消灭民族资产阶级-

摘要:Objective:The use of a combination of tumor markers may be an important tool in the early diagnosis of pancreatic cancer, which is the key to improving prognosis. The study aim was to investigate the diagnostic value of carbohydrate antigen 19-9(CA 19-9) and carcinoembryonic antigen(CEA) levels in patients with pancreatic cancer. Methods:An immunoradiometric assay was used to homochronously measure the serum CA19-9 and CEA levels in 78 pancreatic cancer cases and 64 healthy examinees in hospital. The normal reference values were CA19-9(0-39 U/ml) and CEA(0-3.4 ng/ml). Results: Mean serum CA19-9 and CEA levels in patients with pancreatic cancer(406.55±60.18 U/ml, 12.43±1.25 ng/ml) were significantly higher(P < 0.01) than those in healthy examinees(16.54±1.95 U/ml, 2.37±0.17 ng/ml). The sensitivity of the combined detection of CA19-9 and CEA (92.31%) was significantly higher(P < 0.05) than that of either marker alone(79.49%, 71.79%, respectively). In addition, the sensitivity to diagnose pancreatic cancer by detecting the serum CA19-9 and CEA levels was higher(P < 0.05) in stage ⅡB + Ⅲ + Ⅳ(87.04%, 79.23%) than stageⅠ+ⅡA(62.50%, 54.17%). Conclusion: The combined detection of CA19-9 and CEA could overcome the deficiency of using single marker detection by improving the sensitivity to diagnose pancreatic cancer. At the same time, CA19-9 and CEA detection could be used to assess mesenteric artery invasion and the metastasis of lymphatics and distant organs in pancreatic cancer.

摘要:Objective:To assess the effects of lightwand and laryngoscopic intubation techniques in patients undergoing laparoscopic cholecystectomy(LC). Methods: 300 ASA physical status I and II patients, undergoing LC, were randomly assigned to two groups, with 150 cases in each group. Patients in the LS group underwent endotracheal intubation using a standard direct-suspension laryngoscopic technique. Patients in the LW group were intubated by using transillumination with a lightwand. Mean arterial pressure and heart rate were recorded before induction, and at 1, 3 and 5 min after intubation. The incidence and of sore throat, hoarseness, and dysphagia was assessed twenty-four hours after surgery. Results: This study demonstrated no clinically significant difference in cardiovascular variables between the two techniques. Patients had a significantly lower incidence of sore throat, hoarseness, and dysphagia when the lightwand was used for intubation. Conclusion: This study suggests that lightwand intubation may decrease the incidence of postoperative sore throat, hoarseness, and dysphagia, thereby potentially increasing satisfaction in surgical patients. Therefore, more frequent use of the lightwand is recom-mended for endotracheal intubation.

摘要:“整形美容”已日益成为一个热门话题-文章采用问卷调查法和个案访谈法相结合的方式对福州市女性白领的整形美容时尚消费情况进行实证调查,进而对调查的结果进行社会学分析,希望此调查分析能够大致勾勒出城市女性白领整形美容消费的现状-意愿-困扰因素和动机-

摘要:Objective:To investigate the method and effect of nephron-sparing surgery in the treatment of small renal cell carcinoma. Methods: From August 1997 to October 2008, 48 cases of small renal cell carcinomas were confirmed by surgery and pathology, and reviewed retrospectively. Of the 48 cases, there was 1 patient with bilateral tumors, 8 with solitary kidney tumors, 1 with unilateral tumor and a damaged contralateral kidney, and 38 with unilateral tumors and a normal contralateral kidney; 9 underwent tumor enucleation and the remaining patients received partial nephrectomy. Results:There were no local tumor recurrences and/or tumor metastasis at a mean follow--up of 60 months. Conclusion: Confirming conclusions from other centers, we have found that nephron-sparing surgery is an effective treatment for small renal cell carcinomas.

摘要:血液是生命之源,输血是一种支持与代偿性的治疗措施,《献血法》颁布实施至今9年来,我国无偿献血事业取得了举世瞩目的成就-但是,与人民群众健康需求相比,我国无偿献血工作还存在许多问题和不足-为了有效地预防和控制血源性疾病的传播,保护供血者和受血者的身体健康,必须进一步加强公民无偿献血的法制建设-

摘要:在传统的医疗过失侵权诉讼中,为了体现行外人士对医疗这一专家型行业的尊重,医学判断在诉讼中发挥着重要的甚至决定性的作用-知情同意诉讼,虽被归入医疗过失侵权诉讼,却提供了一个不同于传统的医疗领域——诊断治疗——的情景-知情同意的独特性改变了医学判断在诉讼中的格局-以患者为取向的信息披露标准的建立意味着法律的关注点已从“专家”整体转移到“患者”;对“医学判断”所管辖领域的精细分割也说明,医学判断只应在证明医疗信息的感知和启用“医疗特权”上有应用价值,医疗信息的实质性判断已让位于一般人知识,而且对“医疗特权”的严

摘要:Primary germinoma in the spinal cord is very rare. Preoperative diagnosis is important because germinomas are sensitive to radiation therapy and chemotherapy so that treatment trends and prognosis differ from other intramedullary spinal cord tumors. Preoperative radiologic diagnosis is very difficult because of this rarity. In this case a 22-year-old woman with a 4-month history of gradual numbness and weakness of both lower extremities was found to have a solid tumor in the thoracic cord between the T9 and T11 vertebral bodies. No other neoplastic lesion was found inside or outside the central nervous system. The patient underwent surgery, and the intramedullary lesion was almost totally resected. Serum HCG was elevated postoperatively without pregnancy. The pathological diagnosis was conclusively that of a germinoma. The previous 23 cases of primary spinal cord germinoma were reviewed for comparison.

摘要:文章就非那根案例,围绕美国食品药品管理局(FDA)与州侵权法庭的法律优先适用和政府监管与侵权救济的问题展开讨论-根据FDA“有效改变规则”,该案实则不存在法律优先适用问题,但基于此的争论对《联邦药品食品化妆品法》与州侵权法,乃至药品的政府监管与侵权救济的关系的探究有着重要意义-

摘要:目的:通过医用卫生材料招标采购这几年来的实践情况进行数据分析,客观评价当前医用卫生材料招标采购制度实施的情况-效果-存在的问题及原因,提出解决方案及对策-方法:采用比较分析法,以南京市的招标前后的数据进行对比分析;对专家进行咨询,对招标采购相关人员进行访谈-结果:医用卫生材料使用量增长速度很快,医院总收入增长与医用卫生材料支出相关程度高,医用卫生材料增长速度与高值耗材增长速度相关程度高-2003~2007年南京市医用卫生材料集中招标采购中,部分产品价格有明显下降-结论:医用卫生材料招标采购政策方向是对的,

摘要:目的:从循证护理角度探讨健康教育对慢性阻塞性肺疾病的疗效影响-方法:检索主要医学数据库,按一定标准筛选文献并进行质量评价,采用Review Manager软件从遵医行为-圣·乔治呼级系统问卷(SGRQ)评分-肺功能检查-知识掌握-住院天数-住院次数-就诊次数-6分钟步行测试等8个方面进行Meta分析-结果:在遵医行为-SGRQ评分-肺功能检查(FEV1/FVC%)-住院天数方面健康教育组与对照组之间存在显著性差异,而在肺功能检查(FEV1-FEV1%)-知识掌握-住院次数-就诊次数-6分钟步行测试方面两组

摘要:了解医科大学在校女生生殖健康知识的掌握情况以及相关的行为-态度和需求,为教育者和管理者提供可靠的依据-方法:采用整群抽样的方法,对某医科大学一-二-三年级在校女生1 858人进行问卷调查-结果:医科大学在校女生掌握了一定的生殖健康知识,但对于常见的避孕方法“皮下埋植”,知晓率只有14.8%;知道非淋菌性尿道炎是性病的仅占16%-4.1%的人有过性生活,7.2%的人有过手淫行为-50%以上赞成婚前性行为,19.4%赞成性开放-95.7%认为有必要对大学生进行生殖健康教育-结论:医科大学在校女生的生殖健康知识

摘要:政府承担着保护和促进公民健康的责任,公立医院作为政府举办的非营利性医疗机构,在政府履行健康责任中发挥着重要的平台作用-文章对政府通过公立医院来实现其健康责任的方式与机制进行了探讨和研究-

摘要:文章从患者日益增长的就医需求出发,应用Delphi研究方法,通过对江苏省30位医院管理-医学研究方面的专家进行调研,匿名问卷统计分析,冀求建立三级医院人性化医疗服务评价指标体系-对于完善人性化医疗服务的模式-提供人性化医疗服务的制度保障,提高医疗服务质量具有重要的意义-

摘要:目的:探讨医院信息系统中医嘱执行单对护理工作的帮助与存在的不足-方法:基于现有的医院信息系统,根据日常工作的需求,引入医嘱执行单的使用-信息系统自动生成提供执行时间确认-医嘱执行单打印-结果:信息系统自动生成的医嘱执行单,数据准确,内容全面,分类清晰-经抽样统计,使用该执行单后处理医嘱时间与之前相比有显著的缩短(P < 0.05)-结论:使用信息系统自动生成医嘱执行单,有效减少了工作时间,是提高工作效率的一种行之有效的方法-

摘要:目的:了解南京某三级甲等医院本科学历护士的科研认识及态度,为加强护士的科研培训提供依据-方法:对南京某三级甲等医院已取得本科学历的245名护士进行科研认识及态度的问卷调查,对所收集的资料进行SPSS11.5统计软件处理-结果:护士科研认识总得分12~24分,平均(20.69±3.07)分-影响科研认识的主要因素是最初学历,即初始学历是本科组对护理科研重要性的认识比最初始学历是中专组和大专组充分-护士科研态度总得分为2~8分,平均(6.84±1.09)分-结论:本科护士对护理科研的重要性有较充分的认识,对护

摘要:道德教育是一个完整的-有机的形态,具有其生态本性-文章从此逻辑起点出发,提出道德教育研究本身具有其生态性,拘泥于某一特定学科视角局限的道德教育研究缺乏全局性的总体把握-因此,从道德教育的生态整体性出发,从伦理学-教育学-社会学-心理学等多学科视角,从支持系统-操作系统-环境系统和转化系统建构方面,全面分析道德教育,建构起多学科研究视野中的道德教育生态体系,是解决当前道德危机和德育困境的重要途径-

摘要:明末理学家朱用纯的《朱子家训》在中国历史上广为流传,脍炙人口-在当今多元文化的时代背景下,朱子家训对大学生的养成教育-感恩教育与心理教育均有积极的现实意义-

摘要:文章从隐性知识与创新能力培养的关系入手,初步探讨在医学人才创新能力培养中,发挥隐性知识作用的重要性,提出推进导师制-建立创新型学习组织和加强实践教学等建议-

摘要:高校学生社团日益成为校园文化建设的主阵地,社团文化对于和谐校园建设具有不可替代的重要作用-学生社团建设应准确定位-科学引导-创新机制-以生为本-彰显和谐,不断克服制约其作用发挥的消极因素,保护多样性-突出主体性-彰显平等性-扩大开放性,在体现多元价值诉求-保持身心健康-促进人际和谐-实现知行统一等方面推动学生和谐个性成长,为和谐校园建设提供科学理念-文化内核-制度保障-动力机制和良好文化生态-

摘要:临床诊疗通常是由症状导向疾病,强调症状诊断-这要求医学生能够从某一症状联想到可能与之相关的疾病,通过发散性和逆向思维进行诊断和鉴别诊断-但目前临床医学教学过程往往由系统疾病的病理机制引申出相关症状,与临床诊疗实践相反,因此要注重培养医学生的发散性和逆向诊断思维-文章拟对医学生发散性和逆向诊断思维能力的培养进行初步探讨-

摘要:目的:研究前列腺素E2(PGE2)对人肝细胞癌HepG2细胞E-cadherin表达的调节作用及其分子机制,探讨其与肝癌侵袭转移的关系,为肝癌的治疗提供新的研究思路-方法:COX-2瞬时转染人肝细胞癌HepG2细胞,Western blot实验检测其E-cadherin表达的变化;PGE2及PI-3K抑制剂LY294002处理人肝细胞癌HepG2细胞,应用Western blot和RT-PCR分别测定肝癌细胞内E-cadherin蛋白水平和mRNA水平的变化,并观察60 min内Akt磷酸化水平的变化-结果:COX-2过表达转染HepG2细胞后,E-cadherin的表达明显降低;5-10-20 μmol/L PGE2处理HepG2细胞24 h后,E-cadherin表达水平与对照组比较分别下降了22.31%-36.3%-47.83%(P < 0.05);20 μmol/L PGE2处理HepG2细胞 24-48-72 h,E-cadherin蛋白和mRNA的表达与对照组比均明显降低(P < 0.01)-PI-3K抑制剂LY294002能部分阻断PGE2对E-cadherin表达的调节作用;经PGE2处理的HepG2细胞Akt磷酸化水平显著升高,并且在15 min时达到最高-结论:PGE2可以抑制人肝细胞癌HepG2细胞中E-cadherin的表达,并很可能通过激活Akt信号转导通路而发挥作用-

摘要:目的:观察密码子优化能否增强乙型肝炎病毒表面抗原中蛋白核酸疫苗的免疫原性-方法:根据乙型肝炎病毒(adr亚型)表面抗原中蛋白(MHBs)的氨基酸序列,在不改变其氨基酸序列的基础上,设计并人工合成了密码子优化的基因,并将该基因克隆到核酸疫苗载体pSW3891中,构建了密码子优化的表达MHBs 核酸疫苗(命名为:pSW3891/MHBs/adr/opt,简称opt)-用上述核酸疫苗与表达野生型MHBs核酸疫苗(命名为:pSW3891/MHBs/adr,简称adr)及空载体质粒pSW3891一起瞬时转染293T 细胞,应用蛋白质印迹法检测转染细胞中MHBs的表达-采用肌肉注射法,以opt-adr及pSW3891,分别对BALB/c小鼠进行免疫-用ELISA方法检测免疫后小鼠血清中HBs抗体的滴度,ELISPOT法检测免疫小鼠表面抗原特异性分泌 IFN-γ的脾细胞-结果:opt和adr均可在体外293T 细胞中高效表达,且opt的蛋白表达量要高于野生型-opt免疫组小鼠特异性抗体滴度和免疫小鼠表面抗原特异性分泌 IFN-γ脾细胞数量都要显著的高于adr免疫组小鼠-结论:密码子优化可以增强乙型肝炎病毒DNA疫苗在体外的蛋白表达量及免疫小鼠的体液免疫和细胞免疫原性-

摘要:目的:研究睾丸去神经支配对性激素水平的影响,探讨精索神经对精子发生的调控机制-方法:健康成年雄性SD大鼠48只,随机分为假手术组(SO)-精索上神经切除组(SSN)-精索下神经切除组(ISN)和精索上神经+精索下神经切除组(SSN+ISN),每组12只大鼠-解剖显微镜下建立睾丸去神经支配大鼠模型,分别于术前7天-术后1-7-14-21-28天于大鼠眼眶后静脉丛处取血,放射性免疫的方法检测血清中睾酮(T)-卵泡刺激素(FSH)-黄体生成素(LH)-泌乳素(PRL)和雌二醇(E2)水平-结果:术后1天各组血清T水平均显著下降(P < 0.05),术后7天回升,此后去神经组T水平持续下降(P < 0.05),SSN+ISN组较SSN组和ISN组下降更为显著(P < 0.05),SO组无明显变化(P > 0.05);术后1天各组血清LH水平均显著升高(P < 0.05),术后7天稍有回落,此后去神经组LH水平持续上升,SSN+ISN组较SSNG和ISN组上升更为显著,SO组无明显变化(P > 0.05);各组血清中FSH水平均于术后1天显著升高(P < 0.05),后逐渐恢复至术前水平;去神经组血清中PRL水平于手术后显著升高(P < 0.05),SO组无明显改变(P > 0.05);术后去神经组血清E2水平均显著下降(P < 0.05),SO组仅术后1天显著下降(P < 0.05),后恢复到术前水平(P > 0.05)-结论:精索神经对多种性激素水平具有调节作用,这可能是其实现生精调控作用的重要途径之一-

摘要:目的:初步探索人类免疫缺陷病毒1型(HIV-1)反式激活蛋白(Tat)对卡波济肉瘤相关疱疹病毒(KSHV)感染人神经母细胞瘤细胞系SK-N-SH细胞(SK细胞)的影响-方法:将原发性渗出性淋巴瘤(PEL)细胞系BCBL-1细胞与SK细胞共培养,观察SK细胞的细胞病变效应(CPE)并采用Real-time PCR检测KSHV在SK细胞中的复制-重组质粒Tat+pcDNA3.1(+)和空载体pcDNA3.1(+)分别转染SK细胞,经G418筛选获得抗性克隆,以RT-PCR和Western blot检测Tat基因的表达-通过BCBL-1细胞与SK-Tat/SK-vector细胞共培养,采用Real-time PCR检测SK-Tat/SK-vector细胞中KSHV裂解期基因ORF50和26的mRNA表达-结果:细胞共培养后,SK细胞出现CPE且SK细胞中检测到KSHV的基因-RT-PCR和Western blot均在Tat预期位置检测到特异性条带-Real-time PCR检测显示Tat降低KSHV ORF50和26 mRNA转录水平-结论:初步探索表明,在细胞共培养过程中Tat蛋白抑制KSHV在SK细胞中的复制-

摘要:目的:克隆人类疱疹病毒8型(human herpesvirus 8,HHV-8)ORF50截短基因和编码病毒IL-6(vIL-6)全长基因,将其置于大肠杆菌中作融合表达,并纯化融合表达的vIL-6-方法:分别以本实验室先前构建的重组质粒pcDNA3.1+ORF50和pcDNA3.1+vIL-6为模板,PCR扩增目的基因片段,克隆到原核表达载体pGEX-6p-1中,构建含HHV-8 ORF50截短基因的重组原核表达质粒pORF50-C1,pORF50-C2,pORF50-C3和含vIL-6全长基因的重组原核表达质粒pvIL-6-重组质粒经酶切鉴定和核酸序列测定正确后转化大肠杆菌(E.coli)BL21(DE3)感受态细胞,以异丙基硫代-β-D-半乳糖苷(IPTG)诱导表达融合蛋白-用glutathione Sepharose 4B 亲和层析柱纯化vIL-6表达产物-用SDS-聚丙烯酰胺凝胶电泳(PAGE)和Western blot检测GST融合蛋白的表达和纯化情况-结果:核酸序列分析的结果表明,克隆的序列与基因数据库中已登记的HHV-8相应序列呈现100%同源;IPTG诱导后的菌体,经SDS-PAGE,显示有相应大小的融合表达蛋白产生-Western blot 亦在预期的位置检测到特异性条带-结论:在E.coli中获得了正确表达的HHV-8 ORF50基因3个截短片段和vIL-6全长基因,并成功纯化了vIL-6融合蛋白-

摘要:目的:观察成年ROSA26小鼠中枢神经系统半乳糖苷酶(X-Gal)的表达与分布-方法:应用X-Gal组织化学结合尼氏染色及免疫组织化学双标技术研究β-gal在该嵌合体脑内的表达与分布特征-结果:X-Gal组织化学染色显示在该线系小鼠脑和脊髓中均广泛表达转基因β-gal活性产物,但以灰质为主-X-Gal 和尼氏双重染色表明,β-gal在神经元-室管膜细胞和脉络丛上皮细胞中均有表达-免疫组化进一步显示β-gal广泛表达于神经元中,但在星形胶质细胞中则未见表达-结论:ROSA26小鼠中枢神经细胞稳定而广泛的表达β-gal, 此转基因模式动物将可用于神经细胞培养-移植及其它相关神经生物学研究-

摘要:目的:探讨Bmi-1在抗皮肤衰老中的作用-方法:使用组织学,免疫组织化学和Western-blot的实验方法比较分析了同窝2周和4周龄Bmi-1基因敲除纯合子小鼠和野生型小鼠皮肤表型的差异-结果:Bmi-1纯合子小鼠出现皮肤变薄-角质层增厚-皮下脂肪和胶原减少等衰老表型-通过增殖细胞核抗原(PCNA)免疫组织化学染色,发现Bmi-1纯合子小鼠中PCNA阳性细胞较同窝野生型小鼠明显减少-Western-blot结果显示细胞周期依赖性激酶抑制因子p16-p19和p27在Bmi-1纯合子小鼠皮肤组织中都明显升高-结论:Bmi-1能够通过抑制细胞周期依赖性激酶抑制因子的表达,刺激皮肤细胞增殖而发挥抗皮肤衰老的作用-

摘要:目的:建立小鼠急性乙型肝炎病毒感染动物模型,在此基础上观察慢病毒载体携带小干扰RNA(small interfering RNAs,siRNAs)对乙型肝炎病毒(HBV)复制和表达的影响-方法:36只Balb/c小鼠随机等分为3组(n = 12):①空白对照组(NC组):通过尾静脉注射pTHBV2(包含HBV全基因组真核表达质粒),建立小鼠急性HBV感染模型;②干扰组(E组):将pTHBV2与pWPT-HBV-siRNA1(携带靶向HBV S区的siRNA1的慢病毒)通过尾静脉共注射小鼠;③无关干扰组(I组):pTHBV2和pWPT-HBV-siRNA2(携带非靶向HBV的无关siRNA2的慢病毒) 通过尾静脉共注射小鼠-于注射后第4天和第7天取小鼠血清和肝脏组织-采用ELISA法检测血清中HBsAg(HBV表面抗原);选择逆转录聚合酶链反应方法(RT-PCR)检测肝组织HBV S-mRNA水平;用免疫组织化学法检测肝组织HBcAg(HBV核心抗原)和HBsAg-结果:与NC组比较,E组小鼠血清中HBsAg水平在第4和第7天分别下降89.76%和94.81%(P < 0.01);RT-PCR结果提示E组肝组织HBV S-mRNA水平明显降低(P < 0.05);免疫组织化学法检测结果显示E组肝组织HBcAg﹑HBsAg阳性细胞数明显减少(P < 0.05)-I组与NC组比较,上述检测指标无统计学差异(P > 0.05)-结论:慢病毒载体携带siRNAs能够显著抑制小鼠体内HBV的复制和表达,并且抑制作用在第7天较第4天更强,没有减弱的趋势-

摘要:目的:研究大鼠的肠缺血预处理对肝脏缺血再灌注损伤(HIRI)的远端保护作用及其机制-方法:30只大鼠随机分为3组,假手术组(Sham组)仅做肝门分离;缺血再灌注组(IR组)采用肝门阻断法(Pringle’s法)缺血30 min,再灌注120 min,建立缺血再灌注模型;远端预处理组(RIPC组)阻断肠系膜上动脉10 min,再灌注10 min,然后同IR组-分别取门静脉血清测肝酶(ALT-AST)-内毒素;取肝-肠组织分别作病理学检查-MPO活性测定;取肝组织作免疫组化TNF-α和细胞间黏附分子-1(ICAM-1)测定-结果:RIPC组较IR组:①血清ALT-AST-内毒素减少(P < 0.05);②肝和肠组织损伤病理评分-MPO活性降低(P < 0.05);③肝组织内TNF-α和ICAM-1表达减少-结论:对大鼠肠的缺血预处理可以发挥远端预处理作用,减轻肝脏的缺血再灌注损伤,可能是通过减少肝脏前炎性因子的表达,减少中性粒细胞的黏附聚集而发挥作用-

摘要:目的:构建检测抗原特异性细胞毒性T淋巴细胞的单链MHC-肽四聚体-方法:通过在MHC重链基因中引入生物素化位点,并在轻链和重链基因中引入同一限制性酶切位点连接MHC轻链和重链构建可生物素化的单链MHC分子-结果:重组子以包涵体形式表达得到单链MHC分子,重折叠-生物素化-荧光标记后得到活性单链MHC-肽四聚体-结论:单链MHC分子操作简便,提高了四聚体构建效率-

摘要:目的:研究经脾脏注射未成熟树突状细胞(iDC)对大鼠小肠移植免疫耐受的诱导-方法:健康成年F344大鼠为供体,SD大鼠为受体-随机分为4组(n = 10),A组(阴性对照组):移植前7天,受体经阴茎背静脉注射生理盐水1 ml;B组(阴性对照组):移植前7天,受体经脾脏注射生理盐水1 ml;C组(iDC阴茎背静脉注射组):移植前7天,受体经阴茎背静脉注射供体的iDC,细胞数为2 × 106个;D组(iDC脾脏注射组):移植前7天,受体经脾脏注射供体的iDC,细胞数为2 × 106个-4组1周后行大鼠异位节段性小肠移植术-观察各组受体存活情况,移植小肠采用病理学检查及细胞凋亡检测-结果:D组大鼠移植小肠存活时间为(17.50 ± 1.05)天,较A组(7.17 ± 1.17)天-B组(7.33 ± 1.51)天-C组(12.83 ± 2.79)天显著延长;D组移植小肠炎性细胞浸润-黏膜组织结构破坏程度和黏膜细胞的凋亡数目明显低于A-B-C组-结论:经脾脏注射供体来源的iDC,可在一定程度上诱导受体产生免疫耐受,显著延长受体大鼠小肠移植术后的存活时间-

摘要:目的:探讨肠内免疫营养与生态营养对胃癌术后患者营养状态和免疫水平的影响-方法:将60例胃癌患者双盲-随机分为普通肠内营养组(enteral nutrition,EN组,15例)-普通肠内营养+生态营养组(enteral microeco-pharmaceutics nutrition,EMN组,15例)-肠内免疫营养组(immue-enhancing enteral nutrients,EIN组,15例)-肠内免疫营养组+生态营养组(enteral microecopharmaceutics immunonutrition,EMIN组,15例),比较4组患者的术后营养状况-免疫功能-氮平衡以及菌群失调情况-结果:EIN组与EN组相比,EIN组能提高术后IgG-IgA水平,减少肠管内大肠杆菌-球杆菌含量,增高双歧杆菌-乳杆菌含量(P < 0.05),EMN组与EN组相比,EMN组能提高术后IgG-IgA水平,减少肠管内大肠杆菌-球杆菌含量,增高双歧杆菌-乳杆菌含量(P < 0.05),EMIN组与EN组相比,EMIN组能提高术后总蛋白-白蛋白-前白蛋白-IgG-IgA,IgM-CD3-CD4以及CD4/CD8水平,加速纠正负氮平衡,减少肠管内大肠杆菌-球杆菌含量,增高双歧杆菌-乳杆菌含量(P < 0.05),EMIN组与EMN组-EIN组相比,EMIN组术后总蛋白-白蛋白-前白蛋白-IgG-IgA,IgM-CD3-CD4以及CD4/CD8水平-氮平衡指数,肠管内双歧杆菌-乳杆菌含量明显高于EMN组-EIN组,肠管内大肠杆菌-球杆菌含量明显低于EMN组-EIN组(P < 0.05)-EN组-EIN组以及EMN组的术后总蛋白-白蛋白-前白蛋白-IgM-CD3-CD4-CD4/CD8水平以及净氮平衡指数差异无统计学意义(P > 0.05)-结论:胃癌术后,联合应用肠内免疫营养与生态营养对胃癌术后患者营养支持,与单独使用肠内免疫营养或者生态营养相比,能更好的促进患者蛋白质合成-免疫功能恢复-纠正负氮平衡以及肠道菌群失衡-

摘要:目的:观察不同浓度脂多糖(lipopolysaccharides,LPS)对离体小鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)增殖功能的影响-方法:分别采取野生型和Toll样受体4(toll like receptor4,TLR4)基因缺失小鼠骨髓以贴壁分离法培养骨髓间充质干细胞,取第3~6代的MSCs,通过流式细胞术检测MSCs表面标志显示CD34-CD45阴性;CD105-CD29-CD44阳性-以不同浓度LPS(0.1-1.0-5.0-10.0-20.0 -滋g/mL)分别与野生型小鼠MSCs共培养24 h和48 h,采用Cell Count Kit-8比色法观察LPS对MSCs增殖功能的影响,此外采用TLR4基因缺失小鼠MSCs与LPS 1.0 -滋g/ml共培养,探讨LPS促进MSCs增殖的可能机制-结果:LPS(0.1~5.0 -滋g/ml)能显著提高离体野生型小鼠MSCs的增殖能力(P < 0.05),LPS浓度在5.0 -滋g/ml时对MSCs增殖功能影响最大,随着LPS浓度的继续增大,MSCs的增殖功能反呈下降趋势,但LPS 20.0 -滋g/ml组增殖能力仍高于对照组(P > 0.05);LPS对LR 4基因缺失小鼠MSCs增殖能力无显著影响-结论:适量的LPS能增加MSCs的增殖能力,机制可能是通过激活Toll样受体信号通路实现的-

摘要:目的:探讨迷迭香酸对小鼠单侧输尿管梗阻(UUO)模型肾间质纤维化的抗氧化保护作用-方法:将雄性C57BL/6J小鼠随机分成3组:假手术对照组(Sham组,n = 8)-UUO模型组(结扎左侧输尿管制作UUO模型,n = 24)-UUO迷迭香酸干预组(UUO+RA组,n = 24)-除Sham组外,其余2组根据不同的观察时间细分为3-7-14天亚组(n = 8)-术后第3-7-14天分别处死各组小鼠,组织病理学方法观察肾小管间质损伤情况;Western blotting方法检测肾组织中波形蛋白(Vimentin)和E-钙黏蛋白(E-cadherin)表达;比色法测定小鼠左肾皮质匀浆中脂质过氧化标志物MDA以及抗氧化酶SOD的含量-结果:UUO模型组与假手术组比较:随着模型损伤时间的延长,肾间质损伤程度逐渐加重, Vimentin和MDA含量明显增高,E-cadherin和SOD含量明显降低-迷迭香酸干预组与UUO模型组比较:3天时肾脏病理-Vimentin-E-cadherin以及MDA-SOD含量无明显改变;7天时肾间质纤维化减轻,Vimentin- MDA含量降低,E-cadherin-SOD含量增高;14天时肾间质纤维化明显减轻,Vimentin-MDA含量明显降低,E-cadherin-SOD含量明显增高-结论:迷迭香酸可能通过减少单侧输尿管梗阻侧肾皮质脂质过氧化物的产生-增加抗氧化酶的含量,从而改善小鼠氧化应激所致的肾间质纤维化-

摘要:目的:制备并构建神经导向因子netrin-1(NT-1)和EGFP双基因共表达的重组腺病毒载体,为后续的基因转染做准备-方法:采用基因克隆技术克隆目的基因NT-1,并将目的基因克隆入含有报告基因EGFP的穿梭载体pDC316质粒;构建的质粒pDC316-netrin经酶切及测序鉴定正确后,通过脂质体lipofectamine2000的介导与腺病毒包装质粒pBHGlox_E1.3Cre共转染至人胚肾细胞HEK293,经同源重组后得到携带鼠NT-1的重组腺病毒Ad5-netrin-CMV-EGFP-应用PCR鉴定重组腺病毒,空斑传代纯化病毒并反复冻融扩增病毒-以50%组织培养感染剂量法(TCID50)测定病毒滴度-结果:PCR鉴定证实重组腺病毒含有鼠NT-1基因,病毒滴度为5.2 × 109pfu/ml,EGFP活性检测腺病毒感染阳性细胞率在40%以上-结论:成功制备Ad5-netrin-CMV-EGFP腺病毒,为以后基因治疗研究打下基础-

摘要:目的:研究大鼠孕期维生素D(VitD)缺乏对其子鼠长骨中维生素D受体(VDR)蛋白表达的影响-方法:成熟雌性SD大鼠36只随机分为模型组和对照组各18只,模型组以不含VitD的大鼠饲料避光喂养,对照组以标准饲料正常光照喂养-2周后与成熟SD雄性大鼠交配,分别取孕20天(E20d)-生后1天(B1d)和生后7天(B7d) 子鼠左侧股骨-用Western blotting方法检测子鼠股骨VDR蛋白水平的表达-结果:VDR蛋白在E20d-B1d和B7d子鼠股骨的表达量,模型组均显著低于对照组(P均 < 0.05)-结论:孕鼠VitD缺乏可下调子鼠长骨VDR蛋白表达水平,可能为子鼠骨骼发育不良机制之一-

摘要:目的:观察TNF-α诱导Molt-4细胞的凋亡现象以及cyclin B1的表达,探讨膜受体途径细胞凋亡机制-方法:用TNF-α处理指数生长期的Molt-4细胞;应用API-免疫印迹电泳等方法检测细胞凋亡和cyclin B1的表达-结果:Molt-4细胞经TNF-α诱导后出现了明显的细胞凋亡,细胞凋亡主要发生在细胞周期的G1期,cyclin B1在G1期呈阳性表达-结论:TNF-α诱导的细胞凋亡具有细胞周期特异性,cyclin B1在G1期细胞中表达升高可能与G1期细胞凋亡有关-

摘要:目的:探讨接受早期介入治疗的高危急性冠脉综合征(ACS)患者,血小板膜糖蛋白(GP) Ⅱb/Ⅲa受体拮抗剂的最佳应用时机-方法:选择接受早期冠脉介入治疗(PCI)的高危不稳定型心绞痛/非ST段抬高心肌梗死(UA/NSTEMI)患者162例,按替罗非班使用时机分常规早期治疗组和延期选择性治疗组,分别评估两种治疗方案对PCI术前-术后心肌组织水平灌注和30天复合心血管事件发生率的影响-结果:PCI术前TMPG分级0~1级患者共65例(40.1%),其中早期治疗组27例(32.9%),延期治疗组38例(47.5%);统计显示早期治疗组PCI术前TMPG分级0~1级发生率显著低于延期治疗组(P < 0.05)-30天复合心血管事件及出血发生率两组无差异(P > 0.05)-结论:GP Ⅱb/Ⅲa拮抗剂早期治疗能改善PCI术前心肌微循环,提高组织水平灌注-

摘要:目的:研究静脉注射重组人脑利钠肽(rhBNP)治疗急性心肌梗死伴心力衰竭患者的疗效-方法:2006年8月~2008年8月住院的急性心肌梗死伴心力衰竭患者81例,随机分为常规治疗组(n = 43)和rhBNP治疗组(n = 38),常规治疗组:予病因-强心-利尿-扩血管治疗,rhBNP治疗组:常规治疗基础上加静脉注射rhBNP[90 s匀速静脉注射冲击量1.5 μg/kg, 0.0075 μg/(kg·min)维持静脉注射48 h]-应用USCOM无创血流动力学监测系统监测各组治疗前-治疗后30 min-1-3-6-12-24和48 h的血流动力学参数和48 h的尿量-血肌酐-血钾-血钠-结果:rhBNP静脉注射后外周血管阻力(SVR)较治疗前下降(P < 0.05), 每搏输出量(SV)﹑心脏指数(CI)﹑总尿量(UR)较治疗前升高(P < 0.05),血肌酐(CR)较治疗前下降(P < 0.05),较之常规治疗组的变化更明显-结论:急性心肌梗死伴心力衰竭患者静脉注射rhBNP较之单纯常规治疗有着更好的血流动力学效应和临床效果-

摘要:目的:评价使用丙酸氟替卡松的哮喘患者,其β2-肾上腺素受体基因(ADRB2)Arg16Gly的变异对沙美特罗临床反应性的影响-方法:62例哮喘患者,给予丙酸氟替卡松/沙美特罗(FSC)(100 -滋g/50 -滋g),2次/天吸入,治疗12周,治疗结束后有2~4天的导出期;以DNA直接测序法,确定62例ADRB2 5个位点的基因型及单倍型-结果:①哮喘在沙美特罗治疗期间,无论Arg16Gly为哪一种基因型,哮喘控制指标如峰值呼气流速(PEF)-1秒钟用力呼气容积(FEV1)-沙丁胺醇的使用量以及哮喘症状评分较其基础值都有持续地明显地改善(P < 0.001),Arg/Arg患者的PEF较其基础值增加了(114.4 ± 21.5)L/min,Gly/Gly与Arg/Gly患者分别增加了(100.3 ± 14.7)L/min和(103.3 ± 23.7)L/min,但3种基因型的哮喘控制指标的变化的比较,差异无显著性(P > 0.05);其他的3个哮喘指标也有相似的改变;②对沙美特罗的反应性不受单倍型配对的影响(P > 0.05);③在导出期,所有患者的哮喘控制指标都有相似的下降,但无基因型方面的差异(P > 0.05)-结论:在长期吸入糖皮质激素的情况下,Arg16Gly基因型或单倍型不会影响对沙美特罗的治疗反应性-然而,目前尚需要大规模的前瞻性的临床药物遗传学研究以及遗传流行病学研究来进一步阐明-

摘要:目的:使用蛋白芯片技术检测患者血清中的抗ENA抗体谱,评价蛋白芯片技术检测抗ENA抗体谱的性能-方法:收集249例患者血清,用自身抗体IgG检测蛋白芯片进行抗ENA抗体谱检测,应用PBT型生物芯片识别系统进行图像分析,以免疫印迹方法作为对照-结果:自身抗体IgG检测蛋白芯片与免疫印迹法方法相比,符合率分别为:抗SS-A/Ro抗体82.2%-抗SS-B/La抗体88.2%-抗Scl-70抗体94.7%-抗Jo-1抗体95.9%-抗Sm抗体92.5%-抗u1RNP抗体88.5%-抗Rib-P抗体82.2%-结论:自身抗体IgG检测蛋白芯片可应用于抗ENA抗体谱检测,与免疫印迹技术相比,检测结果无显著性差异-

摘要:目的:探讨困难型腹腔镜胆囊切除术(laparoscopic cholecystectomy,LC)的处理-方法:回顾性分析292例困难型腹腔镜胆囊切除术-结果:急性胆囊炎198例,其中结石嵌顿172例,炎症发作超过72 h的37例;困难型慢性胆囊炎94例,其中,慢性萎缩性胆囊炎40例,慢性结石嵌顿68例-中转开腹7例(2.4%),其中主动中转6例,被动中转开腹1例-手术时间35~210 min,平均(64.13 ± 27.83)min,术中出血20~400 ml,平均(52.60 ± 27.16)ml,放置腹腔引流管201例-结论:困难型LC术中,把握好困难型LC的原则及技巧,则安全且可行-

摘要:目的:应用咪唑安定-丙泊酚作镇痛镇静辅助在电子结肠镜下对老年患者进行息肉治疗,评价其疗效和安全性-方法:将140例结肠镜下进行息肉电切治疗老年患者随机分为2组:镇静组(80例)静脉注射咪唑安定和丙泊酚,至患者进入睡眠状态后进行结肠镜检查及息肉治疗;对照组(60例)按常规进行结肠镜下息肉治疗-观察2组检查前-检查中-检查后的血压-脉搏-呼吸-指血氧饱和度以及检查反应和进镜时间-检查成功率-结果:镇静组操作过程中可见一过性血压和心率下降,与检查前比较有显著性差异,但检查结束后又恢复原来水平;对照组检查过程中均有不同程度的血压升高-心率加快,与检查前比较有显著性差异;两组操作过程前-中-后的SpO2变化无显著性差异,镇静组进镜时间和检查反应明显优于对照组-结论:丙泊酚和咪唑安定辅助结肠镜检查进行老年患者结肠息肉治疗是安全有效的,其进镜时间和检查反应优于常规结肠镜下进行息肉治疗-

摘要:目的:探讨复杂困难胆囊行腹腔镜切除术的可行性和安全性-方法:回顾分析本院自2002年1月~2007年12月施行腹腔镜胆囊切除术(laparoscopic cholecystectomy,LC)1 188例,其中复杂切除困难的169例,分析其手术治疗效果及优点-结果:169例复杂困难胆囊行腹腔镜胆囊切除术中,158例在腔镜下完成,均采取胆囊管逆行分离法切除成功,中转11例,总体中转开腹率6.92%;合并胆总管损伤1例,胆漏1例,出血1例,戳孔感染3例,随访2~24个月,胆总管残余结石3例,经ERCP取石成功-除中转开腹者,余术后 4~6 天痊愈出院,疗效满意-结论:采用逆行法腹腔镜胆囊手术对复杂困难胆囊行切除,只要方法适当,是安全可行的,必要时可中转开腹处理-

摘要:目的:探讨多排螺旋CT血管成像对于腹腔干及其分支变异的显示方法及其准确性-方法:回顾性分析2008年1月~9月行上腹部CTA成像患者180例,采用多平面重建-最大密度投影-容积再现显示腹腔干及其分支起源-走行;记录各种重建方法显示腹腔干及其分支的解剖变异情况-结果:所有患者的腹腔干及其分支血管显示均满意;其中13例患者有腹腔干及其分支血管的变异-容积再现显示全部13例患者的解剖变异,最大密度投影显示8例,曲面重建未能显示任何变异情况-结论:CT血管成像可以清晰地显示腹腔干及其分支变异;在显示变异血管方面,容积再现优于其他后处理方法-

摘要:目的:了解近年南京儿童医院下呼吸道感染性疾病中肺炎克雷伯菌的感染现状-产超广谱-茁-内酰胺酶(ESBLs)及耐药情况-方法:回顾分析本院微生物室2004年1月~2007年12月从痰标本中分离的肺炎克雷伯杆菌共计1 786株,进行kirby-bauer(简称K-B)琼脂扩散法抗生素敏感实验,按照2004~2006年每年美国临床实验室标准化委员会标准进行判读,并计算该细菌的耐药率(R%)-结果:2004-2005-2006-2007年分别送检痰标本966-3 758-7 802-9300份,阳性率为53.31%-48.56%-49.29%-54.53%-肺炎克雷伯杆菌分离率为21.74%-14.46%-17.70%-14.37%,产ESBLs率为69.64%-62.50%-65.41%-72.01%-对亚胺培南非产ESBLs肺炎克雷伯杆菌和产ESBLs肺炎克雷伯杆菌均高度敏感,耐药率 < 4%,对头孢美他醇的耐药率稳定在22%~32%之间,且有下降趋势-产ESBLs肺炎克雷伯杆菌对常用的-茁-内酰胺类抗生素高度耐药-对头孢哌酮/舒巴坦的耐药率由2005年的52.63%逐渐下降为2007年的8.26%-结论:肺炎克雷伯杆菌是儿童下呼吸道感染的主要病原,其中产ESBLs肺炎克雷伯杆菌占大多数,表现为多重耐药-对产ESBLs肺炎克雷伯杆菌所致重症感染可首选碳青霉烯类或抗生素与酶抑制的复合制剂治疗-

摘要:目的:研究水中分娩对新生儿的影响-方法:2005年6月~2008年5月,选择无妊娠合并症和并发症的单胎-头先露初产妇,其中自愿选择水中分娩52例为观察组,另52例选择产床分娩为对照组,比较两组新生儿出生后1-5 min Apgar评分,新生儿脐动脉血pH-PO2-PCO2值,出生后2-5天新生儿行为神经评分(neonatal behavioral neurological assessment,NBNA)和耳拭子细菌培养结果-结果:水中分娩组和对照组新生儿Apgar评分-脐动脉血气分析和NBNA评分差异无显著性(P > 0.05),两组入新生儿病房治疗发生率差异无显著性(P > 0.05),新生儿耳拭子培养均为阴性,无新生儿感染发生-结论:水中分娩新生儿未见不良结局-只要严格掌握适应证,遵守操作流程,遵循无菌操作的原则,助产得当,水中分娩对新生儿是安全的-

摘要:目的:比较波前像差引导LASIK手术和常规LASIK手术矫治近视的效果-方法:2003年6月~2007年6月行波前像差引导的LASIK手术及同期行常规LASIK手术的所有患者,排除门诊随访数据不全的患者-年龄18~50岁,平均(24.53 ± 3.56)岁,屈光度-2.00~-12.00D,按屈光度分组:A组(-2.00~-3.00D)-B组(-3.25~-6.00D)-C组(-6.25~-10.00D)-D组(-10.00D以上)-复查术后1个月和6个月的视力-总体像差-对比敏感度-屈光度-结果:①视力及屈光度 :波前像差引导的LASIK手术在各组中均较稳定,而常规LASIK手术的视力在A组-B组较稳定, C组-D组有回退;②像差RMS值:波前像差引导的LASIK手术与常规LASIK手术比较在A组无差别,在B组-C组-D组有显著性差别;③对比敏感度(CS1-CS2-CS3-CS4):波前像差引导的LASIK手术与常规LASIK手术比较A组在4种条件下无差别,B组-C组在CS2-CS3条件下下降,D组在CS1-CS2-CS3-CS4条件下有显著性差别-结论:波前像差引导下的LASIK手术矫治中高度近视具有更好的稳定性和较好的视觉质量-

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