• 2009年第29卷第6期文章目次
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    • >南京医科大学学报(英文版)
    • Evaluation of the adjuvanticity of artemisinin with soluble Leishmania major antigens in BALB/c mice

      2009, 29(6):359-372. DOI: 10.7655

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      摘要:Objective: To determine the adjuvant potential of artemisinin with a soluble leishmanial antigen in vaccinating BALB/c mice.Methods: Seventy two female BALB/c mice were randomly assigned into six groups. The mice were vaccinated with soluble Leishmania antigens (SLA) alone, artemisinin co-administered with SLA, SLA and Bacille Calmette Guérin (BCG) vaccine, and artemisinin and BCG alone. Unvaccinated mice formed the control group. The induction of cell-mediated immunity following vaccination was determined by measuring in vitro lymphocyte proliferation and the production of interleukin (IL)-4, IL-5 and gamma interferon (IFN-γ) determined by flow cytometry. Protection against L. major was determined by quantifying parasite burdens in L. major infected footpads using a limiting dilution assay and by measuring lesion sizes of the infected footpad compared to the contralateral uninfected footpad. Results: Mice receiving SLA plus artemisinin produced significantly high levels of IL-4 and IL-5 (P < 0.05) and low levels of IFN-γ, resulting in exacerbated disease. In addition, subcutaneous administration of SLA + artemisinin, artemisinin alone or SLA alone resulted in the development of large footpad swellings and high parasite loads that were comparable to those of the control unvaccinated mice (P > 0.05), resulting in exacerbated disease. Conclusion: These data suggest that artemisinin is not a suitable adjuvant for Leishmania vaccines. However, since artemisinin has been shown to be effective against Leishmania parasites in vitro and in vivo, further studies ought to be conducted to determine its immunochemotherapeutic potential when co-administered with Leishmania antigens.

    • Effects of Panax notoginseng saponins on apoptosis induced by hydrogen peroxide in cultured rabbit bone marrow stromal cells via altering the oxidative stress level and down-regulating caspase-3

      2009, 29(6):373-379. DOI: 10.7655

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      摘要:Objective: To investigate the effects of Panax notoginseng saponins (PNS) on hydrogen peroxide (H2O2)-induced apoptosis in cultured rabbit bone marrow stromal cells (BMSCs). Methods: The effects of different concentrations of PNS on proliferation and early osteoblast differentiation of BMSCs were determined by the MTT assay and an alkaline phosphatase (ALP) assay. An optimal effective concentration of PNS was determined and used in subsequent experiments. The cultured BMSCs were divided into three groups: untreated control, H2O2 treated, and PNS pretreatment of H2O2 treated. The oxidative stress level was assessed by superoxide dismutase (SOD) and malondialdehyde (MDA) assays. Flow cytometry was used to determine BMSC apoptosis by staining with annexinV-FITC/propidium iodide (PI). The activity of caspase-3 enzyme was measured by spectrofluorometry. Results: PNS (0.1g/L) significantly increased both BMSC proliferation rate and ALP activity, while it decreased the indicators of oxidative stress, caspase-3 activity, and the apoptosis rate of BMSCs induced by H2O2.. Conclusion: PNS, acting as a biological antioxidant, had a protective effect on H2O2-induced apoptosis in cultured rabbit BMSCs by decreasing oxidative stress and down-regulating caspase-3.

    • Effect of Interferon-alpha in systemic lupus erthematosus (SLE) serum on the differentiation and maturation of dendritic cells derived from CD34+ hematopoietic precursor cells

      2009, 29(6):380-385. DOI: 10.7655

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      摘要:Objective: To study the effect of interferon-alpha IFN-α in the serum of SLE patients on the differentiation and maturation of dendritic cells (DCs) derived from CD34+ hematopoietic precursor cells (HPCs). Methods: Serum samples from SLE patients and normal controls were collected and the concentration of IFN-α detected by ELISA. CD34+HPCs were purified from cord blood by a magnetic cell sorting system (MACS), and cultured to differentiate to DCs. Normal serum, normal serum with exogenous IFN-α, SLE serum with raised levels of IFN-α, or SLE serum with anti-IFN-α neutralizing antibody was added to the culture medium. The phenotype of DCs was analyzed by flow cytometry (FCM) and the capacity of DCs to stimulate allogenic T lymphocyte proliferation was evaluated in a mixed lymphocyte reaction by the Cell Counting Kit-8. Cytokine production was assessed by ELISA. Results: Serum levels of IFN-α were significantly higher in SLE patients than in normal controls and this correlated positively with disease activity. Cultured in SLE serum with raised levels of IFN-α, CD34+ HPCs could differentiate into DCs that expressed higher levels of HLA-DR, CD80 and CD86, and showed an enhanced allogenic T-cell stimulatory capacity, while producing lower levels of IL-12 and higher amounts of IL-10 compared with those DCs cultured in normal serum. Conclusion: Increased levels of IFN-α in SLE serum promotes the differentiation and maturation of DCs derived from CD34+ HPCs and could contribute to the pathogenesis of SLE.

    • Curcumin induces the expression of NF-κB and Bcl-2/Bax in human renal cell carcinoma cell line ACHN

      2009, 29(6):386-391. DOI: 10.7655

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      摘要:Objective: To explore the in vitro effects of curcumin on the proliferation and apoptosis of the human renal cell carcinoma cell line ACHN, and to investigate its mechanisms of action. Methods: The human renal cell carcinoma cell line ACHN was treated with different concentrations of curcumin for 24 h. The MTT assay was used to evaluate the cytotoxic effects of curcumin and flow cytometry was utilized to observe and detect the apoptosis of ACHN cells induced by curcumin. The expression levels of Bcl-2, Bax and NF-κBP65 mRNA were evaluated by Reverse Transcription-Polymerase Chain Reaction (RT-PCR), while the expression of Bcl-2, Bax, NF-κBP65 and IκB proteins was evaluated by Western blot. Results: The concentrations of curcumin used significantly inhibited the proliferation of ACHN human renal cell carcinoma cells in vitro in a dose and time-dependent manner (Ftime=5.55, P < 0.05; Fdose=110.05, P < 0.05). Obvious apoptosis of cells treated with different concentrations of curcumin could be observed by FCM. Compared with the control group, the apoptosis rates of curcumin-treated cells were markedly increased (F=96.35, P < 0.05). Lower dose of curcumin significantly induced the apoptosis of ACHN cells. With intervention of different concentrations of curcumin (0, 10, 20 and 40 μmol/L) for 24 h, the expression levels of Bcl-2 and NF-κBP65 mRNA in ACHN cells were decreased while the expression level of Bax mRNA was increased (P < 0.05), and Bcl-2, and NF-κBP65 protein decreased, while Bax and IκB protein increased compared with those in the untreated group. Conclusion: Curcumin inhibited proliferation and increased apoptosis of the human renal cell carcinoma cell line ACHN. These curcumin effects appear to involve up-regulating IκB, down-regulating NF-κB, and regulating the expression of the apoptosis genes Bcl-2/Bax.

    • Efficacy of gefitinib as a first-line single agent treatment in patients with advanced non-small cell lung cancer

      2009, 29(6):392-397. DOI: 10.7655

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      摘要:Objective: To assess the efficacy and toxicity of gefitinib as a single agent treatment in Chinese patients with advanced non-small cell lung cancer (NSCLC). Methods: Forty-five patients with advanced NSCLC were treated with gefitinib at 250 mg daily until the disease progressed or the patient could not tolerate the toxicity. Results: None of the patients achieved a complete response (CR), while 15 patients achieved a partial remission (PR) and 17 experienced a stable disease (SD). Thirteen patients continued to have a progressive disease (PD). The response rate and the disease control rate were 33.3% and 71.1%, respectively. The symptom remission rate was 72.5%, and the median remission time was 8 days. The median survival time was 15.3 months. The median progression-free survival time was 6.0 months. The most common toxicities included rash (53.3%) and diarrhea (33.3%). Dehydration and pruritus of the skin developed in 26.7% and 22.2% of the patients, respectively. Hepatic toxicity occurred in 6.7% of patients and oral ulceration occurred in 4.4% of patients. Conclusion: Single agent treatment with gefitinib is effective against advanced NSCLC, and is well tolerated in Chinese patients.

    • Inhibition of cellular proliferation and induction of apoptosis in human esophageal carcinoma cell lines by extracts of Dioscorea bulbifera L and Chinese Angelica

      2009, 29(6):398-402. DOI: 10.7655

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      摘要:Objective: To investigate the antiproliferative and apoptogenic activities of polysaccharides extracted from Dioscorea bulbifera L (DBP) and Chinese Angelica (CAP) and a 3:2 mixture of these compounds (DCCP) on two esophageal carcinoma cell lines, EC9706 and Eca109. Methods: A MTT assay was used to detect the effects of DBP (10 μg/ml and 100 μg/ml), DCCP(17 μg/ml and 170 μg/ml) and CAP (10 μg/ml and 100 μg/ml) on the proliferation of EC9706 and Eca109 cells. DNA content analysis by flow cytometry was used to determine the cell cycle distribution, and Annexin V-FITC/PI stained fluorescence-activated cell sorter (FACS) was used to detect the apotosis rate of treated cells. Western blots were used to examine protein levels. Results: DBP and DCCP strongly inhibited the proliferation and viability of both the EC9706 and Eca109 cells. CAP enhanced the effects of DBP. DCCP primarily arrested the EC9706 and Eca109 cells at the G1 phase of the cell cycle. DCCP induced apoptosis in both esophageal carcinoma cell lines, and reduced the expression of pIκBα and Bcl-2 proteins. Conclusion: DCCP triggered apoptosis in esophageal carcinoma cells by inhibiting the NF-κB signaling pathway.

    • Verapamil inhibits 3T3-L1 preadipocyte differentiation

      2009, 29(6):403-409. DOI: 10.7655

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      摘要:Objective: To investigate the effect of the calcium channel blocker verapamil on adipocyte differentiation and its mechanism of action. Methods: Preadipocytes from 3T3-L1 strain mouse embryos were cultured and differentiated into matured adipocytes in vitro. Verapamil was added to the culture medium in the concentration of 30 μmol/L on Day 0. Cell differentiation was determined by Oil Red O staining and marker gene mRNA expression was evaluated and compared by RT-PCR. The fluo-3/AM probe and laser scanning confocal microscopy were used to measure intracellular calcium concentrations. Results: ①The differentiation rate of 3T3-L1 preadipocytes exposed to verapamil was lower than that of untreated cells. ②Verapamil promoted the retention of pref-1 gene expression. Lipoprotein lipase expression in the verapamil group was significantly lower than that in the control group on Day 4, Day 6 and Day 8 (P < 0.05) and resistin expression was significantly lower than that in the control group on Day 6, Day 8 and Day 10 (P < 0.05). Fatty acid synthase expression in the verapamil group was significantly lower than that in the control group from Day 2 (P < 0.05). ③ Intracellular concentrations of calcium [Ca2+]i in the verapamil group were significantly decreased compared with those in the control group on Day 2, Day 4 and Day 6 (P < 0.05), while there was no obvious difference between the two groups on Day 0 (P > 0.05). Conclusion: In 3T3-L1 preadipocytes verapamil significantly reduced adipocyte differentiation, down-regulated the mRNA expression of three marker genes for adipocytes differentiation, and prolonged the mRNA expression of an inhibitor of differentiation. The inhibitory effect of verapamil on differentiation may involve its role as a blocker of calcium influx in adipocytes.

    • Activation of angiotensin II type 1 receptors in the median preoptic nucleus induces a diuretic and natriuretic response in rats

      2009, 29(6):410-414. DOI: 10.7655

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      摘要:Objective: To investigate the effect of activation of angiotensin II (AngII) type 1 (AT1) receptors in the median preoptic nucleus (MnPO) of rats on renal sodium excretion. Methods: After anesthesia, the rats were injected into the MnPO via an implanted cannula. Urine samples were collected via a bladder cannula, and the urine sodium concentration was assayed with flame spectrophotometry. The serum level of endogenous digitalis-like factor (EDLF) and Na+,K+-ATPase activity in the renal cortex tissue were assayed respectively with a radioimmunoassay and with an ammonium molybdophosphate-based kit. Results: Both the urinary volume and the sodium excretion peaked 60 min after AngII was administered into the MnPO. The responses were accompanied by an increase in serum EDLF and a decrease in Na+,K+-ATPase activity in the renal cortex. The responses of diuresis and natriuresis, as well as an increase in serum EDLF and a decrease in Na+,K+-ATPase activity in the renal cortex induced by MnPO adminstration with AngII were inhibited by pior treatment with the AngII receptor blocking agent losartan into the MnPO. Conclusion: These results suggest that activation of AT1 receptors in the MnPO of rat induces diuretic and natriuretic responses. The responses are associated with an increase release of EDLF and with the inhibition of Na+,K+-ATPase activity in renal cortex tissue.

    • Ultrasonic evaluation of the relationship between left ventricular hypertrophy or left ventricular geometry and endothelial function in patients with essential hypertension

      2009, 29(6):415-419. DOI: 10.7655

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      摘要:Objective: To assess the relationship between left ventricular hypertrophy (LVH) or left ventricular geometry (LVG) and endothelial function in patients with essential hypertension (EH). Methods: Seventy-six patients and 30 normal subjects were first examined by echocardiography. Brachial artery dilatation induced by reactive hyperemia (DIRH) or nitroglycerin (DING) was detected using high-resolution ultrasonography. Results: DIRH was lower in patients with hypertension than in the controls, and the decrease in DIRH was greater in the patients with LVH than that in patients without LVH (4.36±2.54% vs 8.56±1.87 %; P < 0.0001). There were no significant differences in age, serum concentrations of total cholesterol, triglycerides or sugar, blood pressure and the brachial artery dilatation induced by nitroglycerin between the two groups (P > 0.05). While there was no significant difference in DIRH between the patients with normal left ventricular geometry or cardiac remodeling, the patients showing either eccentric or concentric left ventricular hypertrophy had lower DIRH than the patients with normal left ventricular geometry or cardiac remodeling. The DIRH was the lowest in patients with concentric hypertrophy. Although bivariate analysis showed that the left ventricular mass index (LVMI) correlated well with the brachial artery dilatation induced by reactive hyperemia, diastolic blood pressure and mean blood pressure (r=-0.61, P < 0.0001; r=0.27, P < 0.05; r=0.31, P < 0.05, respectively), a multivariate stepwise regression demonstrated that LVMI correlated only with the brachial artery dilatation induced by reactive hyperemia. Conclusion: Left ventricular hypertrophy was related to endothelial dysfunction in essential hypertension. The endothelial dysfunction might be basic and important in the progression of left ventricular hypertrophy.

    • Maxillary first premolars:Ⅰ.Morphology of the apical constriction

      2009, 29(6):420-424. DOI: 10.7655

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      摘要:Objective: To investigated the apical constriction morphology of maxillary first premolars in the Chinese population. Methods: Eighty recently extracted human maxillary first premolars from a native Chinese population were used. The number and shape of apical constrictions were recorded under a dental operating microscope (DOM) at 12.5×2.5 magnification. After access preparation, a new K-file was inserted into the canal until the tip of the file was just seen at the apical constriction under the DOM. The teeth with files in the canals were X-rayed from a mesiodistal direction using a direct digital radiography (DDR) system, and the distance between the file tip and the center of radiographic apex was directly measured from the computer screen using DDR measurement software. Results: The percentage of teeth with an apical constriction was 78.5% (102/130). The most common apical constriction shapes were oval (55.9%) and round (35.3%). The mean distance between the apical constriction and the anatomical tip of the root was 0.61 mm, and 84.3% (86/102) were within 1 mm. Conclusion: The most common shape of an apical constriction was oval or round, and the distance to the apex was mostly within 1 mm, indicating that root canal therapy should stop 1 mm from the radiographic apex.

    • Recurrent cervical chordoma: A case illustration

      2009, 29(6):425-429. DOI: 10.7655

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      摘要:A case is described of multi-recurrent cervical chordoma in a man over a 5 year period. The clinical features were of progressive spinal cord compression. The authors report a chordoma at C4 that recurred 3 times in five years. The patient underwent four operations and suffered distant metastases. This case confirms that thorough resection of the tumor during the first surgery and post-operative adjuvant treatment are the best assurance of a good prognosis with a chordoma. Multiple surgeries can stimulate biological activity of a chordoma and make its recurrence and distant metastases much more likely. The authors discuss the diagnosis, surgical treatment and the relationship between the histopathological changes and malignancy of a spinal chordoma after four operations. To our knowledge, this represents the first report of a 4th surgery for cervical chordoma.

    • Treatment of hepatic venous stenosis by transfemoral venous balloon dilation following living donor liver transplantation: a case report

      2009, 29(6):430-432. DOI: 10.7655

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      摘要:Hepatic venous stenosis may be a cause of graft failure in living donor liver transplantation (LDLT). Balloon dilation and metallic frame approaches have been used successfully to treat hepatic venous stenosis. Here, we report the effect of transfemoral venous balloon dilation for treating a child with hepatic venous stenosis after LDLT.

    • >南京医科大学学报(自然科学版)
    • 稳定表达GFP-LC3的RAW264.7细胞系的建立

      2009, 29(6):757-761766. DOI: 10.7655

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      摘要:目的:建立稳定表达GFP-LC3的小鼠巨噬细胞RAW264.7细胞系-方法:构建pcDNA3.1-GFP-LC3真核表达载体,应用转染技术将该质粒导入RAW264.7细胞,用G418筛选稳定表达的细胞系-真核细胞中GFP-LC3的表达分别用荧光显微镜与Western blot方法检测,并利用该稳定表达细胞系观察内质网应激时细胞发生自噬的情况-结果:成功获得2株转染并经G418反复筛选的RAW264.7细胞系,在倒置荧光显微镜下观察可见绿色荧光的表达率在95%以上,Western blot结果证实了GFP-LC3融合蛋白的表达-激光共聚焦显微镜和Western blot均证明内质网应激可以诱导自噬的发生-结论:用pcDNA3.1-GFP-LC3 转染的RAW264.7细胞经G418筛选,可成功建立GFP-LC3稳定表达系,从而为后续功能实验提供有用的细胞研究模型-

    • miR-196a2成熟区单核苷酸多态性对靶基因LSP1表达的影响

      2009, 29(6):762-766. DOI: 10.7655

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      摘要:目的:研究miR-196a2成熟区单核苷酸多态性rs11614913(T/C)对预测靶基因LSP1表达的影响-方法:构建含有不同基因型miR-196a2的真核表达载体pCDNA3.1-miR196a2-C和pCDNA3.1-miR196a2-T;同时利用化学合成含有不同基因型的成熟miR-196a2探针miR-196a2-C和miR-196a2-U-将靶基因LSP1的3’UTR序列克隆至pMIR-REPORTTM Luciferase 载体中而获得LSP1报告基因载体pMIR-LSP1-采用所构建的miR-196a2真核表达载体以及化学合成的成熟miR-196a2 探针分别与靶基因LSP1报告基因载体组建两套转染体系,分别共转染于HEK293-A549和CHO三种细胞后进行荧光素酶活性分析-结果:miR-196a2真核表达载体与靶基因LSP1报告基因表达载体共转染于HEK293-A549和CHO三种细胞后进行荧光素酶活性分析,pCDNA3.1-miR196a2-C等位基因荧光素酶相对活性与pCDNA3.1-miR196a2-T等位基因相比均有显著降低(P < 0.05);含不同基因型的化学合成的成熟miR-196a2 探针与靶基因LSP1报告质粒共转染HEK293- A549和CHO三种细胞,miR-196a2-C等位基因荧光素酶活性与miR196a2-T等位基因相比也有显著降低(P < 0.05)-结论:荧光素酶活性强弱间接反映了miR-196a2与靶基因LSP1结合能力的大小,当miR-196a2 成熟区rs11614913位点为C时,miR-196a2可能更为有效地与预测靶基因LSP1结合,从而在转录后水平调控靶基因表达-

    • 5-Aza-CdR对肺癌A549细胞DNA甲基转移酶活性的影响

      2009, 29(6):767-770778. DOI: 10.7655

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      摘要:目的:研究5-氮杂-2’-脱氧胞苷(5-Aza-2’-deoxycitydine,5-Aza-CdR)处理肺癌细胞株A549后,DNA甲基化转移酶(DNA methyltransferase,Dnmts)转录水平及其活性的变化,以及细胞生长状态的改变-方法:5-Aza-CdR处理A549细胞株,半定量RT-PCR检测癌细胞Dnmts的mRNA转录水平;酶活性连续循环比色法检测Dnmts的催化活性;流式细胞仪(FCM)及MTT法检测细胞的生长状态-结果:Dnmts的转录水平5-Aza-CdR处理前后水平分别为:Dnmt1(1.23 ± 0.253,1.15 ± 0.166)-Dnmt3b(0.760 ± 0.164,0.649 ± 0.181),两组无显著性差异(P > 0.05)-两组Dnmts催化活性分别为:Dnmt1(0.195 ± 0.030,0.153 ± 0.041)-Dnmt3b(0.172 ± 0.029,0.116 ± 0.050),药物组催化活性下降(P < 0.05)-FCM结果提示细胞周期阻滞于G1/G0期,两组凋亡率分别为0.62 ± 0.56,7.60 ± 1.92,凋亡率明显增加(P < 0.01)-MTT结果示药物组细胞生长受抑制-结论:5-Aza-CdR抑制A549细胞株增殖,促进凋亡,对Dnmts的抑制作用是通过降低催化活性实现,不影响其转录水平-

    • 1,25-(OH)2D3在促进大鼠外周血间充质干细胞扩增中的作用

      2009, 29(6):771-774. DOI: 10.7655

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      摘要:目的:探讨大鼠外周血中是否存在非黏附间充质干细胞(MSCs)以及1,25-(OH)2D3能否刺激它们形成成纤维细胞集落形成单位(CFU-f)-方法:从大鼠外周血中分离单核粒细胞(PBMGC)组份,在缺乏和添加10-8 mol/L 1,25-(OH)2D3条件下进行贴壁培养-培养至4天和8天时,将未贴壁细胞转移到新的培养皿中继续培养,即:“pour-off”培养,培养16天形成的细胞进行亚甲基蓝染色,计数CFU-f数目,并对4天“pour-off”培养形成的细胞进行免疫细胞化学染色-结果:PBMGC培养及4天和8天非黏附PBMGC培养都能形成CFU-fs,而1,25-(OH)2D3能够明显地促进它们形成CFU-f-非黏附细胞形成的克隆表达波形蛋白(vimentin)-α-平滑肌肌动蛋白(α-SMA)和Ⅲ型胶原,而不表达Ⅰ型胶原-结论:大鼠外周血中存在非黏附MSCs,在体外培养能够形成CFU-fs,而1,25-(OH)2D3具有促进外周血MSC扩增的作用-

    • 雷公藤甲素对人子宫内膜癌抑制作用的裸鼠体内研究

      2009, 29(6):775-778. DOI: 10.7655

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      摘要:目的:探讨雷公藤甲素(triptolide)对人子宫内膜癌细胞株HEC-1B裸鼠皮下移植瘤的生长抑制作用及其作用机制- 方法:将人子宫内膜癌细胞株HEC-1B细胞接种于裸鼠皮下,建立HEC-1B皮下移植瘤模型,然后随机分为雷公藤甲素高中低剂量组8-4-2 μg/(只·天)和顺铂40 μg/(只·天)及生理盐水对照组共5组,治疗10~15天,观察治疗前后裸鼠体重及移植瘤体积的变化-SABC免疫组织化学染色法检测移植瘤组织中Bcl-2及内皮细胞生长因子(vascular endothelial growth factor,VEGF)的表达-结果:高剂量雷公藤甲素组和顺铂用药组抑制肿瘤作用强,抑瘤率都为50%;高剂量雷公藤甲素组中的Bcl-2及VEGF的表达明显低于生理盐水对照组(P < 0.05)-结论:雷公藤甲素对裸鼠皮下移植瘤有明显的生长抑制作用,其机制可能与其诱导肿瘤细胞凋亡及抑制肿瘤血管形成有关-

    • 提高开胸结扎法建立猪心肌梗死模型成功率探讨

      2009, 29(6):779-783. DOI: 10.7655

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      摘要:目的:探讨提高开胸结扎法建立猪急性心肌梗死模型成功率的方法-方法:幼猪16只,结扎组(10只)复合麻醉,机械通气,正中切口开胸结扎前降支中下1/3,术前-术中-术后静滴-术中浸润利多卡因;假结扎组(6只)穿线不结扎;心电图-饲养3周后冠状动脉造影-心肌核素显像检查-心脏标本HE及切片2,3,5-氯化三苯基四氮唑(TTC)染色-结果:结扎组手术成功率90%-心电图ST段明显抬高;3周后冠状动脉造影提示前降支中远端完全闭塞;核素心肌显像提示前壁-心尖部放射性稀疏或缺如;HE染色见均匀一致的纤维结缔组织增生;切片TTC染色不着色-结论:手术前后静滴及表面浸润利多卡因,正中切口开胸结扎冠状动脉前降支是建立猪心肌梗死模型有效的方法,可明显提高成功率-

    • 福莫特罗和ICI118551对大鼠骨髓间充质干细胞来源的成骨样细胞功能的影响

      2009, 29(6):784-789. DOI: 10.7655

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      摘要:目的:研究选择性β2肾上腺素能激动剂福莫特罗(Formoterol)和阻滞剂ICI118551对体外由大鼠骨髓间充质干细胞(MMSC)成骨分化的影响,探讨β2肾上腺素能受体信号对骨代谢的影响-方法:取3周龄雌性SD大鼠,全贴壁筛选法培养MMSC,用条件培养液诱导向成骨细胞分化后分别加入不同浓度Formoterol和ICI118551,检测细胞碱性磷酸酶,骨钙素的分泌水平和细胞增殖率-结果:MMSC成功诱导分化为成骨样细胞,不同浓度(10-5~10-9 mmol/L)的Formoterol均可抑制成骨样细胞分泌碱性磷酸酶,骨钙素和细胞增殖-高浓度ICI118551(10-5和 10-6 mmol/L)抑制成骨样细胞分泌碱性磷酸酶,骨钙素和细胞增殖,低浓度ICI118551(10-8 和10-9 mmol/L)则可促进成骨样细胞分泌碱性磷酸酶,骨钙素和细胞增殖-结论:β2肾上腺素能受体激动剂可抑制体外成骨样细胞的功能和细胞增殖,而阻滞剂对成骨样细胞功能和增殖的影响与其浓度有关-

    • 白藜芦醇对恶性黑色素瘤生长抑制作用的体外及体内研究

      2009, 29(6):790-793864. DOI: 10.7655

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      摘要:目的:研究白藜芦醇在体外和体内对恶性黑色素瘤的抗肿瘤效果-方法:采用MTT法测定白藜芦醇对小鼠B16及人A375细胞的增殖抑制率;用Western blot方法检测白藜芦醇对B16细胞中p-Akt蛋白表达的影响;建立B16皮下种植瘤小鼠模型,观察不同剂量的白藜芦醇对小鼠皮下瘤的生长抑制作用-结果:体外实验发现在白藜芦醇对B16及A375细胞均显示了良好的浓度依赖性;Western blot显示了不同浓度的白藜芦醇有效的抑制了p-Akt蛋白的表达,提示其抗肿瘤机制可能与此有关;体内实验证实不同浓度的白藜芦醇对恶性黑色素瘤的生长产生了明显的抑制作用-结论:体外和体内实验证实白藜芦醇有效的抑制了恶性黑色素瘤的生长,并且发现其抗肿瘤机制可能通过抑制p-Akt蛋白的表达来实现-这为白藜芦醇在恶性黑色素瘤的临床治疗的可能应用提供了实验基础,也为我国中草药的抗肿瘤效果的开发提供了具体思路-

    • 胰岛素样生长因子-1对胶质瘤C6细胞垂体瘤转化基因的影响

      2009, 29(6):794-796. DOI: 10.7655

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      摘要:目的:探讨胰岛素样生长因子-1(Insulin-like-growth factor-1,IGF-1)在体外对胶质瘤C6细胞垂体瘤转化基因(pituitary tumor transforming gene,PTTG)表达的影响-方法:体外培养胶质瘤C6细胞,给与不同浓度的IGF-1(0.1-1.0-10.0 ng/ml)作用24 h后半定量逆转录聚合酶链式反应(RT-PCR)法检测PTTGmRNA,Western blot检测PTTG蛋白表达-结果:不同浓度IGF-1作用后C6细胞PTTG mRNA表达与蛋白水平均增高,差异有统计学意义(P < 0.01),且随着浓度的增加,其升高越明显-结论:IGF-1可以上调PTTG 的表达,并剂量依赖性-

    • 高糖高脂饮食加链尿佐菌素建立实验性大鼠2型糖尿病模型

      2009, 29(6):797-800806. DOI: 10.7655

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      摘要:目的:高糖高脂饮食加链尿佐菌素诱导实验性大鼠2型糖尿病模型的建立并观察稳定性-方法:60只SD雄性大鼠随机平均分为四组:对照组-链尿佐菌素(streptozotocin)组(STZ组)-高糖高脂饲养组(H.E组)和高糖高脂饲养+STZ组(H.E+STZ组)-STZ组和H.E+STZ组一次性腹腔内注射STZ35 mg/kg-4周后监测体重-胰岛素-血糖-血脂-胰岛素敏感指数(ISI)和葡萄糖耐量实验曲线下面积(AUC)-结果:H.E组和H.E+STZ组大鼠空腹血浆胰岛素-血清甘油三酯(TG)-总胆固醇(TC)-ISI较对照组和STZ组增高(P < 0.01);STZ组及H.E+STZ组大鼠空腹血糖-AUC较对照组和H.E组升高(P < 0.01);注射STZ后4周H.E+STZ组空腹血糖-TG-TC-ISI较对照组和STZ组高(P < 0.01)-结论:高糖高脂喂养能使大鼠胰岛素敏感指数明显下降,并且辅以小剂量链尿佐菌素后2型糖尿病大鼠模型成模率高-稳定性好-效果好-造模时间短-

    • 藤黄酸与草酸铂间的相互作用及机制探讨

      2009, 29(6):801-806. DOI: 10.7655

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      摘要:目的:探讨藤黄酸(gambogic acid,GA)联合奥沙利铂(Oxaliplatin,Oxa)体外对人低分化胃癌细胞株BGC-823和人高分化胃癌细胞株MKN-28的作用及可能机制-方法:采用MTT法测定GA-Oxa单药及联合作用于指数增殖期BGC-823细胞和MKN-28细胞的效应;应用中效原理进行统计分析,绘制单药及联合用药对肿瘤细胞生长的影响曲线,确定联合用药时的效应(Fa)-合用指数(CI)的关系,判定药物间的相互作用;用流式细胞仪检测GA联合Oxa对肿瘤细胞凋亡的影响;采用RT-qPCR Taqman探针方法检测GA对Oxa疗效相关基因ERCC1-BRCA1 mRNA表达的影响,推断GA与Oxa间相互作用的分子机制-结果:GA联合Oxa作用于指数增殖期BGC-823细胞,当Fa= 0.10,CI≈1,两药合用效应相加;当Fa > 0.10,CI < 1,两药合用产生协同效应,凋亡细胞比例明显增加;当Fa < 0.10,两药合用产生拮抗效应;GA联合Oxa作用于指数增殖期MKN-28细胞,当Fa= 0.50,CI≈1,两药合用效应相加;当Fa > 0.50,CI < 1,两药合用产生协同效应;当Fa < 0.50,两药合用产生拮抗效应;GA联合Oxa作用于BGC-823细胞产生了较好的协同效应-GA作用后BGC-823细胞的ERCC1及BRCA1 mRNA明显下调-结论:影响药物疗效相关基因的表达,可能是GA与Oxa联合应用产生协同效应的机制之一-

    • 骨髓增生异常综合征骨髓组织中类胰酶阳性肥大细胞水平的研究

      2009, 29(6):807-810. DOI: 10.7655

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      摘要:目的:探讨骨髓增生异常综合征(MDS)患者骨髓组织中类胰酶阳性的肥大细胞表达水平-方法:用骨髓塑料冷包埋技术结合免疫组织化学的方法检测10例健康供者,8例IDA患者-20例MDS(11例低危组,9例高危组)-17例急性髓性白血病(AML)患者骨髓类胰酶阳性的肥大细胞的数量,并作对比分析-结果:IDA组(63.43 ± 44.29)与健康供者的类胰酶阳性肥大细胞数(61.43 ± 56.19)类似(P值 > 0.05)-MDS组(195.95 ± 117.39)较健康供者类胰酶阳性的肥大细胞数增多(P值 < 0.05)-MDS高危组(229.25 ± 139.68)较低危组(171.73 ± 98.07)高,但无统计学差异(P值 > 0.05));AML组(608.44 ± 342.58)较MDS组明显增多(P值 < 0.01)-各组类胰酶阳性肥大细胞数与外周血常规-骨髓原始粒细胞数-嗜酸性粒细胞数-嗜碱性粒细胞数无相关性-结论:MDS和AML患者骨髓组织中均存在类胰酶阳性肥大细胞数目异常增多现象,它可能在MDS的病理发生发展中起一定作用-

    • 冠心病患者外周血CD4+ Foxp3+ 调节性T细胞与细胞因子水平及临床意义

      2009, 29(6):811-814. DOI: 10.7655

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      摘要:目的:探讨不同类型冠心病患者外周血CD4+ Foxp3+ 调节性T细胞(CD4+ Foxp3+ Treg)与细胞因子的变化及临床意义-方法:分别采用流式细胞术和酶联免疫吸附法(ELISA)检测21例急性心肌梗死-16例不稳定型心绞痛-21例稳定型心绞痛患者外周血CD4+ Foxp3+ Treg百分率及肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)-白介素-10(interleukin-10,IL-10)-干扰素γ(interferon γ,IFN-γ)-白介素-4(interleukin-4,IL-4)-转化生长因子β(transforming growth factor β,TGF-β)的表达水平-结果:①急性冠脉综合征患者外周血CD4+ Foxp3+ Treg百分率显著低于正常人(P < 0.001);②急性心肌梗死患者血浆TNF-α表达水平显著高于稳定型心绞痛患者(P < 0.05),其CD4+ Foxp3+ Treg百分率与血浆IL-10水平呈显著正相关(r = 0.751,P =0.005);③冠心病患者外周血CD4+ Foxp3+ Treg百分率与血浆TNF-α-IFN-γ-IL-4及TGF-β表达水平无显著相关性;④冠心病患者外周血CD4+ Foxp3+ Treg百分率与血清总胆固醇-甘油三酯-高密度脂蛋白-低密度脂蛋白无显著相关性(P > 0.05)-结论:CD4+ Foxp3+ Treg的下降和TNF-α的升高可能参与急性冠脉综合征的发生发展-

    • 丙酸氟替卡松对体外培养鼻黏膜上皮细胞和体内鼻息肉组织中水通道蛋白5表达的影响

      2009, 29(6):815-819. DOI: 10.7655

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      摘要:目的:通过丙酸氟替卡松(FP)对体外原代培养的鼻黏膜上皮细胞和体内鼻息肉组织中水通道蛋白5(AQP5)表达的影响,初步探讨AQP5在鼻息肉发病中的可能机制-方法:将人鼻黏膜上皮细胞进行体外原代细胞培养14天,传代后FP组细胞加入丙酸氟替卡松浓度为0.2%的完全培养液,非FP组细胞加入不含丙酸氟替卡松的培养液继续培养,7天后爬片固定,采用免疫组化技术检测细胞中AQP5的表达-并测定阳性细胞的光密度值和灰度值-将12例慢性鼻窦炎鼻息肉患者(均为初发型),3个月内无局部及全身激素使用史,取其鼻息肉组织,术前应用FP喷雾剂喷鼻7天,治疗7天后术中再次取其鼻息肉组织,术后病理均证实为鼻息肉组织,采用免疫组化技术检测鼻息肉组织中AQP5的表达和分布-并测定阳性细胞的光密度值和灰度值-结果:在体外培养的上皮细胞FP组的AQP5 表达的灰度值明显降低(P < 0.05),光度值则升高(P < 0.05),在体内鼻息肉组织中,患者使用FP后鼻息肉体积明显缩小,,用药后AQP5在腺体细胞和淋巴细胞中表达的灰度值明显升高(P < 0.05),光密度值则明显低于使用前(P < 0.05)-结论:在FP作用下培养的鼻黏膜上皮细胞中的AQP5表达增加,可能解释在体情况下有利于组织间液的排除,减轻中鼻甲和下鼻甲的水肿,而在鼻息肉组织中,应用FP后,鼻息肉体积缩小,水肿减轻,可能与AQP5在腺体和淋巴细胞中表达减少有关,说明AQP5在鼻息肉的形成和发展过程中起到一定的作用-

    • GFAP?NF在原发性脑干损伤后的表达及诊断价值分析

      2009, 29(6):820-824. DOI: 10.7655

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      摘要:目的:研究原发性脑干损伤后胶质纤维酸性蛋白(GFAP)-神经丝蛋白(NF)染色的表达变化及其诊断价值-方法:收集明确死于原发性脑干损伤和脑外疾病者共65例,分为损伤组(25例)和对照组(其中死于心血管疾病20例-非心血管疾病20例),通过对中脑-脑桥和延脑切片,HE染色光镜下观察,免疫组化GFAP- NF染色观察,对所得数据作统计学处理-结果:损伤组中脑干出现星形细胞的急性反应及神经轴索的特征性改变,损伤组与对照组相比,脑干的星形细胞数量及轴索肿胀程度有明显差异(P<0.05)- 结论:上述病理变化对原发性脑干损伤的尸解病理诊断有一定的价值,通过测量星形细胞数量及轴突横径,为脑干损伤导致死亡的诊断提供了一种可参考的量化指标-

    • 双向电泳质谱法研究女性肺腺癌转移淋巴结的蛋白表达

      2009, 29(6):825-830872. DOI: 10.7655

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      摘要:目的:分析肺腺癌转移淋巴结与正常淋巴结组织蛋白质表达差异-方法:运用蛋白质组技术,对肺腺癌转移淋巴结与正常淋巴结组织蛋白质进行分离和比较分析,并在蛋白质和mRNA水平进行进一步验证-结果:获得了背景清晰-分辨率和重复性较好的双向凝胶电泳图谱,比较分析显示与正常淋巴结蛋白质表达水平相比,肺腺癌转移淋巴结有23个蛋白点的表达量存在明显差异,其中新增蛋白点4个,缺失1个,14个蛋白点表达水平明显上调,4个蛋白点表达水平明显下调-共鉴定了11个肺癌转移相关蛋白,其中,候选蛋白膜联蛋白1(ANX1)-细胞骨架蛋白(CK18)-Rho-GDP解离抑制剂1(GDIR)-原肌球蛋白3(TPMF)-蛋白谷氨酰胺γ-谷氨酰转移酶(TGLC)和白介素18(IL-18)在肺腺癌转移淋巴结显著低表达,而候选蛋白核氯离子通道蛋白1(CLI1)-蛋白质二硫键异构酶(ER60)-肌酸激酶-硫氧还蛋白过氧化物酶1(PDX1)和热休克蛋白60(CH60)在肺腺癌转移淋巴结表达水平比正常淋巴结明显提高-结论:肺腺癌转移淋巴结与正常淋巴结蛋白质表达存在明显差异,这11种蛋白质可能与肺腺癌淋巴结转移机制有关-

    • 5-氮杂-2’-脱氧胞苷抑制肾癌细胞增殖及逆转γ-catenin基因甲基化作用

      2009, 29(6):831-835. DOI: 10.7655

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      摘要:目的:研究甲基化抑制剂5-氮杂-2’-脱氧胞苷(5-Aza-CdR)对人肾癌OS-RC-2细胞生长的抑制作用及对γ-catenin基因甲基化状态的影响,初步探讨肾癌发病机制及临床治疗的可行性-方法:不同浓度5-Aza-CdR处理肾癌细胞OS-RC-2后,采用倒置显微镜观察5-Aza-CdR处理OS-RC-2肾癌细胞株前后细胞形态学变化;四唑蓝(MTT)比色观察细胞经药物处理前后的生长活性;流式细胞仪检测5-Aza-CdR对OS-RC-2肾癌细胞株凋亡的影响;Western blot检测5-Aza-CdR处理OS-RC-2肾癌细胞株后γ-catenin蛋白表达的变化;甲基化特异性PCR(methylation-specific PCR,MSP)检测细胞处理前后γ-catenin 基因的甲基化状态-结果:形态学观察显示,用药后癌细胞体积缩小,核固缩,染色质凝聚成块;5-Aza-CdR明显抑制肾癌细胞OS-RC-2的生长;细胞凋亡率增高;药物处理后γ-catenin蛋白表达恢复;未经5-Aza-CdR处理的OS-RC-2细胞中γ-catenin基因启动子区域高甲基化,经10-5 mol/L 5-Aza-CdR处理72 h后,γ-catenin基因启动子区域高甲基化得到逆转-结论:5-Aza-CdR能有效逆转肾癌OS-RC-2细胞γ-catenin基因的异常甲基化,恢复γ-catenin蛋白表达,诱导肾癌细胞凋亡-

    • PLGA/Ⅰ型胶原/壳聚糖复合人工硬脊膜生物相容性及力学性能的实验

      2009, 29(6):836-839. DOI: 10.7655

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      摘要:目的:研究经Ⅰ型胶原-壳聚糖改性的PLGA膜的生物相容性及力学特性,研制出新型人工硬脊膜替代材料-方法:制作PLGA膜(膜Ⅰ)-PLGA/Ⅰ型胶原/复合膜(膜Ⅱ)-PLGA/Ⅰ型胶原/壳聚糖(9 ∶ 1)复合膜(膜Ⅲ)-PLGA/Ⅰ型胶原/壳聚糖(5 ∶ 5)复合膜(膜Ⅳ),并行接触角-吸水率-细胞毒性实验及材料力学实验的研究-结果:接触角:膜Ⅱ < 膜Ⅲ < 膜Ⅳ < 膜Ⅰ,P < 0.01;吸水率:膜Ⅰ < 膜Ⅳ < 膜Ⅲ < 膜Ⅱ,P < 0.01;细胞毒性实验示:第1天,各膜组间OD值差异无统计学意义,P > 0.05-第3-7天,膜Ⅰ与Ⅱ-Ⅲ各组间,膜Ⅲ与膜Ⅳ组间差异有统计学意义,P < 0.05-PLGA膜经Ⅰ型胶原和壳聚糖改性后,可以促进细胞在膜上的黏附-贴壁能力-各膜的力学性能比较,膜Ⅰ与膜Ⅱ-Ⅲ-Ⅳ之间差异有统计学意义,P < 0.05,膜Ⅱ-Ⅲ和Ⅳ组间的差异无统计学意义-结论:PLGA膜的表面复合Ⅰ型胶原/壳聚糖(9 ∶ 1)可以提高复合膜的生物相容性和力学特性-

    • 大鼠局灶性脑缺血再灌注损伤时SB202190的保护作用

      2009, 29(6):840-844. DOI: 10.7655

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      摘要:目的:研究SB202190对大鼠局灶性脑缺血再灌注损伤的影响,探讨SB202190在脑缺血保护中的作用及可能机制-方法:采用线栓法建立大鼠大脑中动脉缺血再灌注损伤模型-雄性SD大鼠,随机分成5组:空白对照组-假手术组-缺血再灌注组-给药组及溶媒组-每组6只大鼠,给药组及溶媒组分别侧脑室注射p38MAPK特异性抑制剂SB202190及1 %DMSO-各组分别进行大鼠神经功能的行为学评分;Nissl染色观察缺血区细胞形态变化;免疫组化检测Bcl-2-Bax阳性神经元变化-结果:缺血再灌注后24 h大鼠神经功能评分显著低于假手术组,有统计学差异(P < 0.05),给药组大鼠神经功能评分显著高于缺血再灌注组,有统计学差异(P < 0.05)-而溶媒组神经功能评分与缺血再灌注组相比无统计学差异(P > 0.05)-再灌注后24 h镜下可见大部分细胞萎缩,胞浆减少,胞核浓染,细胞间隙增大-给药组与缺血再灌注组相比再灌注后24 h细胞形态较好,坏死程度较轻-免疫组化检测结果:空白对照组及假手术组可见极少数Bcl-2-Bax阳性细胞散在分布,两组间无统计学差异(P > 0.05);再灌注后24 h可见大量Bax阳性细胞,与假手术组相比有统计学差异(P < 0.05),而Bcl-2阳性细胞无明显变化(P >0.05);与缺血再灌注组比较给药组再灌注后24 h Bax阳性细胞明显减少,有统计学差异(P < 0.05),Bcl-2阳性细胞与缺血再灌注组比较明显增多,有统计学差异(P < 0.05)-结论:p38MAPK特异性抑制剂SB202190可以改善大鼠局灶性脑缺血再灌注后产生的神经功能缺损症状及神经细胞形态学改变;SB202190能改变局灶性脑缺血再灌注后Bcl-2及Bax的表达,这可能是SB202190抗细胞凋亡的机制之一-

    • 碱性成纤维细胞生长因子对肺癌A459细胞pim-3表达影响的实验研究

      2009, 29(6):845-847. DOI: 10.7655

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      摘要:目的:探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,FGF-2)对肺癌A459细胞株pim-3的影响-方法:不同浓度的(0-25-50-100 ng/ml)FGF-2在体外作用于肺癌A459细胞,半定量逆转录聚合酶链式反应(RT-PCR)法检测pim-3 mRNA,Western blot检测pim-3蛋白表达-结果:与空白对照组比较,RT-PCR 和Western blot检测结果显示pim-3 mRNA及其蛋白的表达在各FGF-2作用组均显著增高,各组间比较差异有统计学意义(P < 0.01)-结论:FGF-2可以量效的方式上调pim-3 的表达-

    • 汉语量词加工的脑功能磁共振研究

      2009, 29(6):848-852. DOI: 10.7655

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      摘要:目的:通过考察大脑在加工量词时的活动情况,探讨量词加工的相关功能区及不同量词功能区可能存在的差异- 方法:对18名健康志愿者行名量词和动量词语义(正确与错误)判断任务,比较不同任务各脑区激活强度及范围的差异-结果:名量词主要激活区域:双侧额中回-左侧额下回-双侧扣带回-双侧楔叶-楔前叶-双侧角回-双侧颞下回-左侧颞中回-舌回-右侧小脑-动量词主要激活的区域:双侧颞中回-双侧颞下回-双侧扣带回-双侧基底节-双侧角回-双侧额中回-左侧额下回-左侧岛叶-双侧小脑-结论:动量词与名量词激活的区域大部分较为一致,两者激活强度上存在差异-但是它们都需要双侧大脑半球各脑区相互协调合作-

    • 卵巢交界性肿瘤39例临床分析

      2009, 29(6):853-856. DOI: 10.7655

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      摘要:目的:探讨卵巢交界性肿瘤的临床特点及治疗方法-方法:回顾分析9年间收治的BOT患者共39例-结果:35例随访至终点的病例中死亡1例,死亡率2.9%-复发5例,复发率14.7%,其中Ia期1例,Ib期1例,Ic期2例,Ⅲ期1例-复发病例中浆液性3例,1例同时有微乳头结构及微浸润-6例肿瘤剥除者中3例复发,18例单侧附件切除者中1例复发-结论:不同FIGO分期是卵巢交界性肿瘤复发的影响因素-手术是卵巢交界性肿瘤的主要治疗方法,早期有生育要求的患者可选择保守性手术,并尽量选择附件切除术;晚期患者宜行根治性手术-

    • 中西医治疗急性胰腺炎52例疗效报告

      2009, 29(6):857-859. DOI: 10.7655

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      摘要:目的:观察中西医结合方法治疗急性胰腺炎的临床疗效-方法:将2005年11~2007年11月间的102例急性胰腺炎患者随机将分为治疗组和对照组,其中治疗组52例,对照组50例;对照组应用西医常规方法治疗,治疗组在对照组基础上以大承气汤为基本方,根据实际情况加味治疗,观察两组用药对急性胰腺炎的临床疗效-结果:治疗组与对照组比较,治愈率与总有效率有显著性提高(P < 0.05)-结论:采用中西医结合方法治疗急性胰腺炎的临床治愈率显著优于单纯采用西医方法治疗,且价格便宜,值得推广-

    • 吉西他滨联合顺铂一线治疗晚期非小细胞肺癌50例临床观察

      2009, 29(6):860-862. DOI: 10.7655

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      摘要:目的:评价吉西他滨联合顺铂(GP方案)一线治疗晚期非小细胞肺癌(NSCLC)的近期疗效及不良反应-方法:对50例均经病理诊断的晚期NSCLC患者采用GP方案化疗,具体为第1-8天吉西他滨1000 mg/m2,静滴30 min;第1-2-3天顺铂25 mg/m2,静滴,21天为1周期,分别化疗2~6周期,后按WHO标准评价疗效和不良反应-结果:总有效率42.0%,中位疾病进展时间和中位生存期分别为6.2个月和10.5个月,主要的不良反应是骨髓抑制和恶心呕吐反应-结论:GP方案一线治疗晚期NSCLC疗效较好,不良反应较轻,值得临床推广-

    • 醒脑静注射液治疗36例小儿脑梗死的疗效明显

      2009, 29(6):863-864. DOI: 10.7655

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      摘要:目的:观察醒脑静注射液治疗小儿脑梗死的疗效-方法:36例脑梗死患儿随机分为两组:醒脑静治疗组(18例)在常规治疗的基础上加用醒脑静注射液,每次0.3 ml~0.5 ml/(L·d),疗程为10天;对照组常规治疗-结果:醒脑静组和对照组治愈率分别为83.3%和44.4%,差异有统计学意义(P < 0.05),两组在意识恢复-肢体肌力恢复-面神经瘫痪恢复-锥体束征消失的时间比较上,醒脑静治疗组明显短于对照组,差异有统计学意义(P < 0.05)-结论:醒脑静注射液在治疗小儿脑梗死疗效好-

    • 纤支镜治疗支气管扩张并感染32例临床观察

      2009, 29(6):865-866. DOI: 10.7655

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      摘要:

    • 牙面湿润方式对复合树脂微渗漏的影响

      2009, 29(6):867-868. DOI: 10.7655

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      摘要:目的:比较不同牙面湿润方式对复合树脂微渗漏的影响-方法:选择24颗离体恒磨牙,在牙体颊舌侧分别制备48个V类洞,随机分为4组-用不同的湿润方式处理酸蚀后的牙面-用Adper Single Bond2 黏接剂,z250复合树脂常规充填固化,经温差循环(5℃~55℃)100次,亚甲蓝染色,双目显微镜下观察牙体剖面充填体与洞壁间龈牙合壁染料渗透情况-结果:4组间鸦壁微渗漏无统计学差异(P > 0.05),4组间龈壁微渗漏有显著统计学差异(P < 0.05),75%乙醇再湿润组龈壁微渗漏明显低于吹干组(P < 0.01)-结论:以乙醇为溶剂的全酸蚀黏接系统,在湿黏接中的湿润方式以75%乙醇溶液棉球湿润洞壁微渗漏最少-

    • 仰卧位颈部手法牵引治疗颈椎间盘突出症20例的疗效观察

      2009, 29(6):869-870. DOI: 10.7655

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      摘要:目的:观察仰卧位颈部手法牵引治疗行颈椎器械牵引有不适反应的颈椎间盘突出症的疗效-方法:20例行颈椎器械牵引有不适反应的患者在药物治疗,物理治疗,颈部制动和推拿的基础上增加仰卧位颈部手法牵引-由专人对每位患者的疗效进行评定和记录-结果:经过30次治疗后,优良率75%,无效率10%,总有效率90%-结论:颈椎间盘突出症在传统的治疗方法中增加仰卧位颈部手法牵引可以明显提高行颈椎器械牵引有不适反应患者的临床疗效-

    • 食管胃颈部吻合术中吻合器的应用体会

      2009, 29(6):871-872. DOI: 10.7655

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      摘要:目的:探讨颈部食管胃吻合时管状吻合器的使用方法-方法:回顾分析48例食管癌患者作食管癌根治术并用管状吻合器进行食管胃颈部吻合的经验-结果:术后吻合口瘘2例,因肺部感染死亡1例;术后吻合口狭窄3例,经扩张后好转-结论:颈部食管胃吻合术中使用管状吻合器具有很好的临床应用价值-

    • 光索导引在急症气管插管中的应用(附87例报告)

      2009, 29(6):873-874. DOI: 10.7655

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      摘要:

    • 应用光学相干断层扫描仪检测垂体瘤患者视网膜神经纤维层厚度

      2009, 29(6):875-878. DOI: 10.7655

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      摘要:目的:探讨光学相干断层扫描仪(optical coherence tomography,OCT)测量视网膜神经纤维层厚度(retinal nerve fiber layer,RNFL)在垂体瘤患者诊断与视功能预后分析中的意义-方法:应用OCT测量正常人76例(76眼)和垂体瘤患者39例(39眼)的各象限和平均RNFL厚度进行比较并计算平均RNFL厚度和视野平均缺损的相关性-结果:垂体瘤患者各象限和平均RNFL厚度均比正常人减少,差异有统计学意义(P < 0.000)-平均RNFL厚度和视野平均缺损呈正相关(R=0.41,P=0.000)-结论:OCT测量RNFL厚度为临床上垂体瘤的诊断提供了一种新的手段-

    • 射频消融治疗高龄患者房室结折返性心动过速

      2009, 29(6):879-881. DOI: 10.7655

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      摘要:目的:回顾性分析射频消融(RFCA)治疗高龄患者房室结折返性心动过速(AVNRT)的有效性和安全性-方法:2005年1月至2008年1月阵发性房室结折返性心动过速的高龄患者21例采用射频消融治疗-结果:21例患者首次消融成功20例,消融成功率95.2%,1例术中失败患者拒绝二次手术,术后共随访9~36个月,均未复发,复发率0%-并发症发生率9.5%,1例为肺气肿患者发生少量气胸,1例放电时出现Ⅰ度房室传导阻滞(PR间期0.28 s),无死亡病例-结论:射频消融治疗高龄患者房室结折返性心动过速是有效的-安全的-

    • 脑胶质瘤ADC值与病理分级的相关性研究

      2009, 29(6):882-884. DOI: 10.7655

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      摘要:目的:探讨磁共振扩散加权成像(DWI)的表面弥散系数(ADC)值对脑胶质瘤的诊断价值-方法:对43例病理证实的胶质瘤患者进行MRI平扫-增强和DWI,分别测量肿瘤的实质部分-肿瘤坏死囊变区-肿瘤周边区域及对侧相应部位正常脑白质的ADC值和相对ADC值-结果:①低级胶质瘤(1~2级)组的肿瘤实质部分的平均ADC值和rADC值均明显高于高级胶质瘤(3~4级)组(P < 0.01)-②胶质瘤实质部分的ADC值和rADC值均与肿瘤组织的病理分级呈明显负相关(P均 < 0.01,r = -0.767和-0.792)-结论:ADC值与胶质瘤的病理分级有明显相关性,可作为胶质瘤术前评价的依据-

    • 彩色多普勒示踪引导鼻空肠管置放术

      2009, 29(6):885-886. DOI: 10.7655

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      摘要:目的:探讨彩色多普勒示踪引导鼻空肠管置放术的可行性-方法:30例拟行肠内营养(EN)支持的ICU住院患者作为研究对象,以Flocare螺旋型鼻空肠管经鼻带导丝置入胃腔,通过尾端注水人工制造多普勒效应,彩色多普勒示踪通过幽门,无强阻力下持续推送直至十二指肠-空肠段,腹部X线平片最终确认鼻空肠管位置后拔出导丝固定鼻空肠管行EN支持治疗-结果:彩色多普勒示踪引导鼻空肠管置放术30例,成功26例(86.7%),失败4例(13.3%),术中所有患者病情均无特殊变化-结论:彩色多普勒示踪引导鼻空肠管置放术是一种操作简便快捷-安全可靠的置管技术,为危重症患者早期肠内营养支持提供了一条更有效的途径-

    • MRI表面弥散系数在诊断乳腺良恶性肿瘤中的价值

      2009, 29(6):887-889. DOI: 10.7655

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      摘要:目的:探讨弥散加权成像(DWI)的表面弥散系数(ADC)鉴别乳腺良恶性病变的价值-方法:对经手术病理证实的36 例乳腺病变(恶性肿瘤20 例,良性病变16 例)进行磁共振DWI检查,并计算病变的ADC 值-结果:20例乳腺癌及16例良性乳腺病变完成弥散成像-恶性肿瘤组ADC 值(0.784±0.178)×10-3mm2/s 明显小于良性病变ADC 值(1.734±0.345)×10-3 mm2/s(P < 0.05,b=800 s/mm2)-结论:ADC 值是鉴别乳腺良恶性病变的一个很有价值的参数-

    • 定量CT肺功能检查对术后肺功能的预测

      2009, 29(6):890-892. DOI: 10.7655

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      摘要:目的:探讨通过定量CT分析(QCT)的方法来预测患者术后的肺功能情况-方法:搜集了22例因肺部肿块行手术切除的患者,术前均行肺动能检查及肺部CT扫描-利用CT肺功能软件,计算出全肺的功能性肺组织的体积(TFLV)和切除肺叶的功能性肺组织的体积(RFLV)-根据术前肺功能检查的数据和TFLV-RFLV得到肺功能的术后预测值-将预测值与术后肺功能的实测值进行Pearson相关分析-结果:预测值与术后肺功能的实测值有较好的相关性-结论:QCT能预测患者术后的肺功能-

    • X线钼靶定位引导下细针穿刺在乳腺微小病变诊断中的作用

      2009, 29(6):893. DOI: 10.7655

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      摘要:目的:探讨乳腺X线定位穿刺留置导丝活检技术对乳腺微小病灶的诊断价值-方法:回顾性分析临床触诊阴性而乳腺钼靶X线片显示的微小病灶36例,采用乳腺钼靶X线定位下穿刺,留置导丝于微小病灶区,引导手术将病灶切除活检-结果:36例微小病灶均一次性定位成功,手术切除完整-病理检查:恶性病变11例,其中浸润性导管癌7例,导管内癌2例,导管内癌伴早期浸润1例,髓样癌1例;良性病变25例-结论:乳腺钼靶穿刺定位活检术定位准确,能确定乳腺微小病灶的性质,是目前诊断早期乳腺癌的有效方法-

    • ICU护士术前访视对心脏手术后焦虑水平的影响

      2009, 29(6):896-897. DOI: 10.7655

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      摘要:目的:探讨ICU护士的术前访视对心脏手术患者术后焦虑水平的影响-方法:将42例心脏手术患者分为观察组和对照组,对照组术前1天进行常规的术前护理和健康教育,同时手术室护士也进行术前访视,观察组除了进行常规术前护理和健康教育,以及手术室护士的术前访视外,还有ICU护士的术前访视-分别于术前1天和术后一周发放焦虑自评量SAS表测评,并比较两组手术前后的焦虑水平-结果:观察组患者术后焦虑水平虽然有所上升,但比对照组焦虑水平低(P < 0.05)-结论:ICU的护士进行术前访视可有效降低患者术后焦虑水平,对促进患者健康有重要意义-

    • 原发性甲状腺恶性淋巴瘤临床病理分析(附6例报告)

      2009, 29(6):898-899. DOI: 10.7655

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      摘要:目的:探讨原发性甲状腺恶性淋巴瘤(PLT)的临床病理特征和鉴别诊断-方法:分析6例原发性甲状腺恶性淋巴瘤的临床资料及组织病理学和免疫表型特点,按WHO(2001)淋巴造血组织肿瘤分类进行组织学分类-结果:PLT多发于妇女,多为单侧或双侧甲状腺肿块-最常见为B细胞性,免疫组化:肿瘤细胞LCA-CD20和CD79α阳性,TG及AE1/3阴性-结论:PLT极少见,确诊依赖组织学和免疫组织化学检测-该病需要和慢性淋巴细胞性甲状腺炎(CLT)及小细胞未分化癌等肿瘤鉴别-

    • 小肠系膜淋巴结滤泡树突状细胞肉瘤1例并文献复习

      2009, 29(6):900-903. DOI: 10.7655

      摘要 (2068) HTML (55) PDF 0.00 Byte (142) 评论 (0) 收藏

      摘要:

    • 897例老年肺部感染病原菌及耐药性分析

      2009, 29(6):904-905. DOI: 10.7655

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      摘要:

    • Mollaret脑膜炎2例报告并文献复习

      2009, 29(6):906-907. DOI: 10.7655

      摘要 (1720) HTML (58) PDF 0.00 Byte (150) 评论 (0) 收藏

      摘要:

    • 彩色多普勒超声诊断动-静脉瘘型KTS综合征1例

      2009, 29(6):907-908. DOI: 10.7655

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      摘要:

    • 数量特征敏感问题分层两阶段整群抽样的统计方法

      2009, 29(6):909-912. DOI: 10.7655

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      摘要:目的:寻求在分层两阶段整群抽样下,数量特征敏感问题的科学调查方法及相应统计公式-方法:数量特征敏感问题无关联随机应答技术(RRT)模型,分层两阶段整群抽样调查方法,均值的性质-抽样理论等统计理论和方法被使用-结果:推导出无关联RRT模型在分层两阶段整群抽样下,总体均值估计量及其估计方差的计算公式;成功应用该方法及相关公式调查得苏州大学学生近两个学期考试作弊平均次数为1.034 8-结论:数量特征敏感问题无关联RRT模型分层两阶段整群抽样调查方法及相应统计计算公式科学可行-