摘要:Vascular smooth muscle cells have attracted considerable interest as a model for a flexible program of gene expression. This cell type arises throughout the embryo body plan via poorly understood signaling cascades that direct the expression of transcription factors and microRNAs which, in turn, orchestrate the activation of contractile genes collectively defining this cell lineage. The discovery of myocardin and its close association with serum response factor has represented a major break-through for the molecular understanding of vascular smooth muscle cell differentiation. Retinoids have been shown to improve the outcome of vessel wall remodeling following injury and have provided further insights into the molecular circuitry that defines the vascular smooth muscle cell phenotype. This review summarizes the progress to date in each of these areas of vascular smooth muscle cell biology.

摘要:司法道德作为适用法律的德性保证,其对法治建设具有不可或缺的意义-在向度上,司法道德表现为追求司法公正-远离司法腐败和忠于法律-唯法是从两个层面-就实现条件而言,司法道德既需要司法工作者个人良好职业操守和外在机制作为保障,也需要学界和司法部门以具有共识性的价值体系对司法活动的引领,还需要司法工作者将司法智慧作为更高层次的价值追求-

摘要:Calcium channel blockers (CCBs) are widely used to treat cardiovascular diseases such as hypertension, angina pectoris, hypertrophic cardiomyopathy, and supraventricular tachycardia. CCBs selectively inhibit the inward flow of calcium ions through voltage-gated calcium channels, particularly Cav1.2, that are expressed in the cardiovascular system. Changes to the molecular structure of Cav1.2 channels could affect sensitivity of the channels to blockade by CCBs. Recently, extensive alternative splicing was found in Cav1.2 channels that generated wide phenotypic variations. Cardiac and smooth muscles express slightly different, but functionally important Cav1.2 splice variants. Alternative splicing could also modulate the gating properties of the channels and giving rise to different responses to inhibition by CCBs. Importantly, alternative splicing of Cav1.2 channels may play an important role to influence the outcome of many cardiovascular disorders. Therefore, the understanding of how alternative splicing impacts Cav1.2 channels pharmacology in various diseases and different organs may provide the possibility for individualized therapy with minimal side effects.

摘要:通过探讨现代生命科技发展造成的伦理问题,挖掘伦理问题形成的根源,对生命科技和生命伦理在一定界域中相对协调-均衡发展进行可行性论证,找到两者动态平衡的契合点,对生命科技的发展实行伦理化管理有针对性地提出了方法论-

摘要:当代社会,大众传媒凭借其无所不在-无所不包和无所不能的嵌入特征,已经成为影响社会和人们生活的重要力量-在现代医学模式下,借助大众传媒的特性,加强医德社会教育,对解决医患矛盾,维护社会稳定,促进社会和谐有着重要作用-具体来说,社会既要利用媒介建设良好的医德信息环境,为医德社会教育提供有力的支撑工具;也要加强医务人员的媒介素养,增强辨别媒介信息的能力和判断信息价值的能力;同时通过行业与大众传媒的合作,坚持正面教育,透过媒介信息显示榜样行为,形成示范效应,便于广大医务人员的选择与仿效-

摘要:Objective: MicroRNA (miRNA) expression is deregulated in many types of human cancers. We sought to investigate the expression patterns of the miRNAs, miR-21, miR-145 and miR-155 in sporadic gastric cancer in a Chinese population. Methods: Total RNA was extracted from archived gastric cancer tissues and adjacent non-cancerous tissues from 20 pairs of paraffin-embedded specimens. Expression levels of miR-21, miR-145 and miR-155 were detected by quantitative reverse transcriptase PCR using a specific stem-loop primer, with U6 as the internal reference gene. Results: The expression of miR-21 and miR-155 in gastric cancer samples was significantly higher than in paired non-cancerous samples (P < 0.05). There was no statistically significant difference in expression levels of miR-145 between cancerous and non-cancerous tissues (P > 0.05). Conclusion: In Chinese sporadic gastric cancer tissues, the expressions of the oncogenic miR-21 and miR-155 were significantly up-regulated, while the expression of the tumor suppressor miR-145 was decreased, although this decrease was not statistically significant. Thus there is specificity in the miRNA expression pattern in gastric cancers in the Chinese population.

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摘要:基于知识创造理论与复杂适应系统理论,剖析医院知识共享的机制;并结合医院管理存在问题,提出医院知识共享的协同管理模式-以促进医院知识共享,提高医院医疗服务水平,实现医院竞争力的提升-

摘要:目的:了解收支两条线管理实施前后上海市松江区公立医疗机构经济运行情况-方法:采用收集公立医疗机构财务报表,再由松江区卫生局统一填写自行设计调查表的方法开展调查-结果:收支两条线管理实施后,上海市松江区公立医疗机构财政补助收入和门急诊人次数逐年增加,业务收入占比和每门急诊人次费用明显下降,但公用支出居高不下,且医疗服务收支赤字持续存在-结论:收支两条线管理保证了公立医疗机构的良好发展,并且促进了其公益性的回归,这种政府为主导的卫生投入机制使基本药物制度的实施有了可能,但公立医疗机构仍需加强预算管理-控制运行成本-

摘要:Objective: To investigate the effect of cardiomyocyte proliferation induced by human hepatocyte growth factor (HGF) in pigs with chronic myocardial infarction (CMI). Methods: A steerable, deflectable 7F catheter incorporating a 27-guage needle was advanced percutaneously to the left ventricular myocardium of 18 pigs with CMI. Pigs were randomized (1:1:1) to receive adenoviral vector HGF (total dose, 1×1010 genome copies), which was administered as five injections into the infarcted myocardium (total, 1.0 mL), or saline, or Ad-null (control groups). Injections were guided by Ensite NavX left ventricular electroanatomical mapping. HGF and cyclin proteins were detected by western blot and immunoprecipitation analysis. Histological and immunohistochemical analysis determined proliferating cardiomyocytes. Myocardial perfusion and cardiac function were estimated by Gated-Single Photon Emission Computed Tomography (G-SPECT). Results: Western blot analyses showed that HGF were predominantly expressed in the infarct core and border in the myocardium of the infarcted heart. G-SPECT analysis indicated that the HGF group had better cardiac function and myocardial perfusion four weeks after the injection of Ad-HGF than before the injection of Ad-HGF. After treatment there were more proliferating cardiomyocytes in the HGF group compared to either of the control groups. Furthermore, the HGF group myocardial samples expressed higher levels of p-Akt, cyclin A, cyclin E, cyclin D1, cdk2, cdk4 than those in the control groups. Conclusion: The over-expression of HGF activates pro-survival pathways, induces cardiomyocyte proliferation, and improves the perfusion and function of the porcine CMI heart.

摘要:随着生活节奏的加快和就业压力的增大,社会已越来越关注职业倦怠,而医护人员因为其职业特点又是职业倦怠的高发群体-本文介绍了我国医护人员职业倦怠的表现,分析其产生的原因,提出了预防医护人员产生职业倦怠的对策-

摘要:本文旨在思考医院文化与核心竞争力的涵义及特征,并阐述了两者之间的关系,进而对文化力建设作为提升核心竞争力的途径进行了探讨-

摘要:This paper first applies the sequential cluster method to set up the classification standard of infectious disease incidence state based on the fact that there are many uncertainty characteristics in the incidence course. Then the paper presents a weighted Markov chain, a method which is used to predict the future incidence state. This method assumes the standardized self-coefficients as weights based on the special characteristics of infectious disease incidence being a dependent stochastic variable. It also analyzes the characteristics of infectious diseases incidence via the Markov chain Monte Carlo method to make the long-term benefit of decision optimal. Our method is successfully validated using existing incidents data of infectious diseases in Jiangsu Province. In summation, this paper proposes ways to improve the accuracy of the weighted Markov chain, specifically in the field of infection epidemiology.

摘要:发展社区卫生服务是政府提供公共服务的一项重要职能,社区卫生人力资源对社区卫生服务的质量有着至关重要的作用-本文主要对徐州市社区卫生服务人力资源现状进行分析,为徐州市社区卫生服务发展提供参考-

摘要:门诊是医院的“窗口”,是接待患者完成一般医疗工作和急救处置的第一线,是患者接触最早-最广泛的场所-门诊公共区域的安全隐患问题关系到医院及医疗公益性事业在人民心中的形象和地位-导医护士是门诊的“窗口”,其在应对门诊公共区域安全隐患中发挥着最直接的和不可替代的作用-

摘要:Objective: To knockdown the C-erbB2 gene in salivary gland adenoid cystic carcinoma SACC-83 cells using RNA interference, and determine the effect of silencing C-erbB2 on cell proliferation. Methods: C-erbB2-siRNA was transfected into SACC-83 cells. RT-PCR and immunohistochemistry were used to detect C-erbB2 expression in SACC-83 cells. Cell proliferation was measured by the MTT assay and gene knockdown was achieved by RNA interference. Apoptosis was analyzed by flow cytometry. Results: Compared with the control, C-erbB2 mRNA expression was decreased in the C-erbB2-siRNA transfection group, and immunohistochemical analysis indicated that C-erbB2 protein expression was decreased. After C-erbB2-siRNA was transfected for 48 h, absorbance at 570 nm (MTT) was 0.185±0.021 compared with 0.354±0.034, 0.299±0.053, and 0.314±0.049 in the blank control, liposome control and negative control siRNA groups, respectively. The differences were statistically significant (P < 0.05) between the C-erbB2-siRNA group and the control groups. Following the C-erbB2 knockdown, the percentage of apoptotic cells was 5.63% compared with 2.04%, 2.85%, and 2.98% in the three control groups, respectively. Proliferation of SACC-83 cells was inhibited, and early apoptotic cells were increased. Conclusion: RNA interference can effectively silence C-erbB2 gene expression and inhibit growth of SACC-83 cells, which indicates the potential of targeting this gene as a novel gene therapy approach for the treatment of salivary gland adenoid cystic carcinoma.

摘要:高等教育体制改革在带给高校办学自主权的同时,也相应地增加了高校的财务风险-在激烈的市场竞争中,一旦财务状况恶化,就会影响高校可持续发展-本文论述了内部控制对防范财务风险的重要作用,分析了现阶段高校财务风险的内部成因,提出了建立和完善高校财务内部控制制度的具体措施-

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摘要:Objective: To investigate the metabolites of polybrominated diphenyl ether 99 (BDE-99) and its related cytochrome P450s in an in vitro system. Methods: Rat primary hepatocytes were isolated and treated with BDE-99 for 24-72 h. Metabolites were then extracted from the hepatocytes and media, and detected by GC/MS. Several mRNAs of metabolic enzymes were also extracted from the same cells and the gene expression levels were determined using quantitative real-time PCR. In addition, selected recombinant cytochrome P450s (CYPs) were expressed in a bacurovirus/sf9 system, and these were further used to explore the metabolism of BDE-99 in vitro. The parent depletion approach was used for screening the ability of CYPs to eliminate BDE-99. Results: A reductively debrominated metabolite, BDE-47, and three oxidative metabolites, 2, 4, 5-tribromophenol, 5-OH-BDE-47, and 5'-OH-BDE-99, were identified from the BDE-99-treated rat hepatocytes, whereas no MeO metabolite was detected in the system. RT-PCR analysis showed that CYP 3A23/3A1, 1A2, and 2B1/2 were induced by BDE-99. Furthermore, using the heterological expressed CYP proteins in in vitro BDE-99 metabolism experiments we found that CYP1A2 and CYP3A4 showed the highest metabolic efficiency for BDE-99, with the metabolic clearance rates of CYP1A2 and CYP3A4 being 30.3% and 27.7%, respectively. CYP1A1 and CYP2A6 displayed relatively low clearance rates, while CYP2E1 seemed not to be associated with the BDE-99 metabolism. Conclusions: In our in vitro rat primary hepatocyte metabolism system, four metabolites of BDE-99 were identified, and CYP3A4 and CYP1A2 were demonstrated to be involved in the BDE-99 metabolism.

摘要:近年来,随着高校科技发展及校办经济产业规模的不断扩大,校办产业存在的问题越来越突出,限制了高校校办产业的发展-文章从校办产业的运行机制与管理体制等方面进行分析,期冀寻求产生问题的根源,并提出相应的对策-

摘要:目的:了解医学院校贫困生的心理健康状况-方法:采用症状自评量表SCL-90和自制“贫困生心理健康状况问卷”作为测试工具,对某医学院校650名贫困生进行问卷调查,与各种常模进行比较,并进行不同类别间的组间差异比较和相关分析-结果:阳性标准≥180分的有68人,占10.6%-医学院校贫困生心理健康状况低于全国成年人水平,优于江苏省大学生水平,心理问题排在前5位的因子是强迫-人际关系敏感-抑郁-精神病性-敌对-不同年级贫困生之间存在显著性差异-年级-经济来源-和周围同学关系-学习成绩的满意度与医学院校贫困生心理健康有显著性相关-结论:医学院校贫困生整体心理健康水平尚属理想,但也有小部分贫困生存在较明显的心理问题-教育工作者应充分考虑年级-成绩等特点,有层次-有区别地进行心理健康教育,以提高医学院校贫困生的综合素质-

摘要:Objective: The cytochrome P450 17α-hydroxylase (CYP17) plays a vital role in androgen biosynthesis. A T-to-C polymorphism in the 5' promoter region of CYP17 has been implicated as a risk factor for prostate cancer, but the results of individual studies are inconclusive or controversial. To derive a more precise estimation of the relationship, we performed an updated meta-analysis from 31 studies based on 27 publications. Methods: A comprehensive search was conducted to examine all the eligible studies of CYP17 polymorphism and prostate cancer risk. We used odds ratios (ORs) with 95% confidence intervals (CIs) to assess the strength of the association. Results: Overall, individuals with CC/CT genotype were not associated with prostate cancer risk (CC vs. TT: OR = 1.03, 95% CI = 0.86-1.24, P = 0.72, Pheterogeneity < 0.0001; CT vs. TT: OR = 0.99, 95% CI = 0.87-1.12, P = 0.88, Pheterogeneity = 0.0006). In the stratified analysis by ethnicity, there was a significantly increased risk of prostate cancer among individuals of African descent under the recessive model (OR = 1.56, 95% CI = 1.01-2.39, P = 0.04, Pheterogeneity = 0.65). Conclusion: This meta-analysis suggested that CYP17 polymorphism might be associated with prostate cancer risk among individuals of African descent.

摘要:医学生临床实习是医学教育过程中的重要组成部分-文章结合临床实际,指出了加强医学生法制观念与实践能力的必要性和重要性,分析了加强其法制观念与实践能力的方法和途径,并提出了合理建议,这对培养高素质医学人才-构建和谐医患关系具有重要意义-

摘要:对于附属医院中的临床医学教育者而言,寻找和开展医学生人文素质教育的有效途径已成为当前的一项重要任务-作者在所在医院的医疗环境和医疗教学条件下,积极探索科学有效的医学生人文素质培养方法和措施,并尝试建立科学的评估标准,在实践中获得了现代临床教学中医学生人文素质教育的有效经验和方法,对今后在临床教学中把医学生人文素质培养变成常规的教学工作有较强的指导意义,同时也为今后建立更加科学化-标准化的医学生人文素质评估方法提供参考-

摘要:面对未来知识社会和终身学习的大趋势,如何指导大学生有效学习逐渐成为世界各国高等学校教育改革的一项重要内容,这也是我国深化高等教育改革的一项重要课题-本文通过对比国内外大学生学习指导的实践与做法,分析了我国大学生学习指导中存在的问题,提出了我国大学生学习指导的实施构想-

摘要:Objective: CYP1A2 and NADPH-CYP450 oxidoreductase (POR) were expressed in the baculovirus/Spodoptera frugiperda (sf9) system. The aim of this study was to investigate the effects of heme precursors on the expression of CYP1A2 and POR. Methods: The heme precursors [δ-Aminolaevulinic Acid (5-ALA), Fe3+ and hemin] were introduced into the system to evaluate their effects on the expression of CYP1A2, POR and their co-expression. All the proteins were identified using immunoblotting, CO-difference spectroscopy, or cytochrome c assay. Results: In the present study, functional CYP1A2 and POR were successfully expressed in the baculovirus/sf9 system, and both of them showed high activities. Co-addition of 5-ALA and Fe3+ significantly improved expression of CYP1A2 by about 50% compared with the addition of 5-ALA, Fe3+ or hemin alone. Either co-addition of 5-ALA and Fe3+ or addition of 5-ALA or Fe3+ alone improved the POR expression level 2 fold and its activity 7-10 fold compared with control (no addition). However, unlike CYP1A2, there was no difference between the co-addition and addition of these heme precursors alone. Different ratios of BvCYP1A2 to BvPOR also affected the co-expression of CYP1A2 and POR, with a 3:1 ratio of BvCYP1A2 / BvPOR significantly increasing their co-expression. Surprisingly, the addition of 0.1 mM 5-ALA or Fe3+ alone, but not their co-addition, could significantly improve the CYP1A2 and POR co-expression (P < 0.05). Conclusion: 5-ALA and Fe3+ increased the expression of CYP1A2 and POR in a baculovirus/sf9 system, but the pattern of their expression was different between their expression alone and co-expression.

摘要:转型时期社会变革的潮流和由此导致的强烈的社会震荡,必然通过社会-学校-家庭等多种渠道向大学生辐射,并使大学生价值取向发生深刻的变化-本次调查以本院大学生的价值取向的现状为切入点,通过对本院大学生价值取向特点及变化原因的探析,提出解决问题的办法和途径,从而达到使本院大学生树立正确的价值观,建立与社会主义核心价值体系相适应的价值取向的目的-

摘要:党内民主是党的生命-加强执政党自身建设必须高度重视扩大党内民主,提高党的基层组织建设科学化水平-在党内民主视野下,协调党内人际关系是党的基层组织建设面临的新课题-协调人际关系要遵循客观规律和基本原则,要掌握和运用协调人际关系的艺术,增强基层党组织服务大局,推动发展,维护和谐的能力-

摘要:Objective: To explore the nature of deficiency in spleen-yin syndrome, which could provide scientific theoretical support and practical guidance for clinical Traditional Chinese Medicine (TCM) syndrome differentiation based on biology, and had a strong clinical significance. Methods: Serum Cu and Zn were detected by atomic absorption spectrophotometer, serum vitamin E by high performance liquid chromatography, serum vitamin C by 2,4-Dinitrophenylhydrazine Colorimetry, total superoxide dismutase (SOD) and Cu and Zn-SOD by the xanthine oxidase method, and malondialdehyde (MDA) by the 2-thiobarbituric acid method (TBA). Total antioxidant capacity was detected by a colorimetry kit. Amylase Activity was detected by an automatic biochemical analyzer. Lysozyme was detected by lysozyme detection plate, the diameter of bacteriolysis circle was measured and the corresponding content of lysozyme was obtained from a table of standard curve values. Results: No significant difference in total SOD and Cu, Zn-SOD was found between deficiency in spleen-yin group and normal group. However, such factors in deficiency in kidney-yin group were significantly lower than the other groups (P < 0.05). The MDA content in both deficiency in spleen-yin group and deficiency in kidney-yin group were significantly higher than that of normal group (P < 0.05), while the total antioxidant capacity was significantly lower than normal group (P < 0.05). The vitamin E content in deficiency in kidney-yin group was significantly lower than that in the other two groups (P < 0.05). No significant difference in the contents of vitamin C, Cu and Zn were observed in these groups. The Zn/Cu level in deficiency in kidney-yin group and the vitamin E level in deficiency in spleen-yin group decreased, but with no significant difference. Amylase activity in unit time in cases with deficiency in spleen-yin was lower than and had significant differences with that in normal cases, and higher than that in cases with deficiency in kidney-yin. The sectional velocity of saliva and the ratio of lysozyme in normal case group were significantly higher than other two groups, while deficiency in the spleen-yin group was significantly higher than the deficiency in kidney-yin group. Conclusion: All the results indicated that the objective pathological mechanism between the deficiency in spleen-yin and deficiency in kidney-yin was different.

摘要:《思想道德修养与法律基础》课案例教学在内容上具有思想性和政治导向性;在结论上具有不同层次的一元性;在形式上具有高度的选择性-其教学环节包括教师选定案例-学生研读案例-学生总结案例-教师点评案例-案例选择应坚持思想性-方向性-感染力三个原则-教师的主导作用-案例自身的说服力和呈现形式的多样性以及学生的主动性和创造性是制约案例教学效果的因素-体现先进的“研究型教学”理念-创建网络案例教学平台-完善教学综合考评体系是提高案例教学效果的有效途径-

摘要:通过我院一年来的医学课程考试方法改革实践,分析了考试改革方案特点:注重课堂表现-见习表现-临床案例分析-过程性评价-总结了成效及问题,最后提出三项解决途径:教师培训-与教学改革同步-长期制度化-

摘要:素质教育是以提高人的综合素质为宗旨的教育,充分发挥解剖专业课基础医学主干课程的作用,全面提高医学生综合能力和素质,培养更多面向现代化-面向世界-面向未来的医学人才-

摘要:目的:研究人类疱疹病毒6型(HHV-6)感染人星形胶质瘤细胞U251,及感染后对其生物学功能的影响-方法:HHV-6感染U251细胞建立HHV-6体外感染模型,倒置显微镜观察细胞病变-PCR法检测HHV-6 u22基因-间接免疫荧光(IFA)检测HHV-6即刻早期蛋白(IE1)和晚期蛋白(gB)的表达-MTT法检测U251感染HHV-6后细胞增殖的改变-流式细胞术(FCM)检测U251细胞周期改变-结果:在感染3天后U251出现典型细胞病变,细胞肿胀增大数倍,透亮,呈多形性-PCR检测到HHV-6 u22基因-IFA检测到HHV-6 IE1蛋白和gB蛋白的表达-MTT法显示HHV-6能增强U251细胞的增殖能力-FCM检测表明,HHV-6感染后U251细胞周期发生改变,感染组和对照组相比,前者G1期的细胞减少,S期和G2期细胞增多-结论:HHV-6能感染U251细胞引起细胞病变,促进细胞的增殖,使细胞周期发生改变-本研究结果提示人类疱疹病毒6型可能参与人神经胶质瘤的发生-发展,对其机制尚不清楚-

摘要:目的:利用慢病毒表达载体将HIV-1 Vif基因导入原发性渗出性淋巴瘤(PEL)细胞BCBL-1中使之持续表达目的蛋白Vif,并检测Vif对其中潜伏KSHV裂解性周期复制的影响-方法:自表达质粒pCI-neo-Vif中扩增出Vif基因,插入到pHAGE-CMV-MCS-IzsGreen中构建成慢病毒载体pHAGE-Vif,利用脂质体将其与包装质粒psPAX2和包膜质粒pMD2.G共转染293T细胞-荧光显微镜观察293T细胞中绿色荧光蛋白(GFP)表达情况;收集培养上清经0.45 μm滤器过滤后即获得病毒悬液-梯度稀释法测定病毒滴度,感染293T细胞,48 h或72 h后进行RT-PCR和Western blot检测Vif基因的转录和表达情况-以MOI为1.0的病毒量感染靶细胞BCBL-1,利用Western blot技术检测Vif蛋白的表达,同时检测KSHV vIL-6和Rta的蛋白表达水平,初步探讨Vif对KSHV复制的影响-结果:限制性内切酶检测和基因测序证实成功构建了携带Vif基因的慢病毒表达载体,滴度为4×107 efu/ml-以MOI为1.0的重组慢病毒感染靶细胞BCBL-1 72 h后,能够检测到外源基因Vif的蛋白表达-Western blot结果初步显示,Vif能够下调vIL-6和Rta的蛋白表达水平-结论:成功构建了含Vif基因的慢病毒表达载体,获得的病毒能够有效感染BCBL-1细胞,并在其中大量表达目的蛋白-初步结果提示,Vif能够下调vIL-6和Rta的蛋白表达水平,对KSHV裂解性周期复制可能起到抑制作用-

摘要:目的:观察重组逆转录病毒人血管内皮生长因子165(retrovirus pLXSN/hVEGF165)基因转染对人骨髓基质细胞(hMSCs)成骨能力的影响-方法:从健康志愿者全骨髓中分离培养人hMSCs,体外扩增纯化后随机分为4组:①retrovirus pLXSN/hVEGF165组:培养液中加入人血管内皮生长因子基因重组逆转录病毒1×1010OPU/ml,孵育24h后换普通培养液继续培养;②retrovirus pLXSN组:培养液中加入逆转录病毒空载体,其余处理与retrovirus pLXSN/hVEGF165组相同;③阳性对照组:培养液中添加地塞米松-抗坏血酸和β-甘油磷酸钠;④空白对照组:不给予特殊处理-各组细胞处理后2周,免疫组化观察VEGF表达,Von Kossa染色检测人hMSCs中骨胶原结节形成,全自动生化分析仪检测培养上清碱性磷酸酶活性-结果:经多次换液传代,人hMSCs呈均一梭形形态-处理后retrovirus pLXSN/hVEGF165组与阳性对照组细胞形态逐渐趋于扁平,突起减少-retrovirus pLXSN组和空白对照组形态无明显变化-免疫组化染色见VEGF主要在胞浆内表达,retrovirus pLXSN/hVEGF165组VEGF表达明显强于其他3组-Von Kossa染色retrovirus pLXSN/hVEGF165组与阳性对照组可见大量红色骨胶原结节形成,显著多于retrovirus pLXSN组和空白对照组-retrovirus pLXSN/hVEGF165组与阳性对照组培养上清液碱性磷酸酶活性显著高于retrovirus pLXSN组和空白对照组-结论:基因重组逆转录病毒人血管内皮生长因子转染对人hMSCs成骨能力具有促进作用-

摘要:目的:齐墩果酸(oleanolic acid,OA)预处理可使大鼠肝糖原增加并使肝脏缺血再灌注损伤(hepatic ischemic reperfusion injury,HIRI)减轻,但机制不详-本研究拟探讨OA预处理对大鼠HIRI前后的PI3K-AKT-GSK-3β信号传导通路的影响-方法:128只SD大鼠随机分为假手术组(SH组)-缺血再灌注组(IR组)-0.5%羧甲基纤维素钠组(CM组)和齐墩果酸预处理组(OA组)-OA组以100 mg/kg的齐墩果酸混悬液,SH和IR组以相同容积的水,CM组以相同容积的0.5% CMC-Na分别每日灌胃1次,连续7天-第8天建立70%肝脏缺血再灌注模型,肝脏缺血60 min后行再灌注-于术前(第8天)-再灌注0-3-6 h取肝组织-用Western blot法测定肝组织的p-PI3K(p85)-p-AKT(ser473)-AKT-p-GSK-3β(ser9)-GSK-3β的蛋白表达量-结果:术前-再灌注0 h时,OA组的p-PI3K(p85)-p-AKT(ser473)-p-GSK-3β(ser9)的蛋白表达量分别比其他3组明显增加(P < 0.05);此时SH组-IR组-CM组的各蛋白表达量之间比较未见统计学差异(P > 0.05)-再灌注3-6 h时,OA组的p-PI3K(p85)-p-AKT(ser473)-p-GSK-3β(ser9)蛋白含量分别比其他3组显著增加(P < 0.05),SH组相应时点的各蛋白含量均比其他3组的含量明显降低(P < 0.05),IR组与CM组相应时点的各蛋白含量之间比较未见差异(P > 0.05);各组的AKT与GSK-3β蛋白含量在各个时间点之间均未见统计学差异(P > 0.05)-结论:OA预处理可使HIRI前后的p-PI3K(p85)-p-AKT(ser473)和p-GSK-3β(ser9)蛋白含量增加,激活肝脏PI3K-AKT-GSK-3β信号通路,此可能是OA减轻HIRI的机制之一-

摘要:目的:探讨PGE2对人肝癌细胞HuH7 VEGF表达的影响及其可能涉及的信号转导通路-方法:用PGE2-EP1-4四种受体激动剂-SQ22536+PGE2-H89+PGE2处理HuH7细胞,Real-time PCR-Western blot-ELISA等检测VEGFmRNA-蛋白表达的变化-结果:10 μmol/L PGE2处理HuH7细胞24 h后,VEGFmRNA的表达水平上升了264%(P < 0.01)-细胞培养上清中VEGF蛋白表达水平上升了24%(P < 0.01);10 μmol/L PGE2处理HuH7细胞6-12-24 h后,胞浆中VEGF蛋白的表达水平分别上升了13.6%-25.7%-39.6% (P < 0.01);10 μmol/L EP2受体激动剂处理HuH7细胞24 h后,胞浆-细胞培养上清中VEGF蛋白表达水平与对照组相比分别上升了32.3%-20.1%(P < 0.01),而10 μmol/L EP1-EP3-EP4受体激动剂处理HuH7细胞24 h后,VEGF的表达水平无明显改变;30 μmol/L PKA抑制剂(H89)-500 μmol/L腺苷酸环化酶抑制剂(SQ22536)处理HuH7细胞24 h后,胞浆VEGF的表达水平分别为对照组的91.6%-89.6%,细胞培养上清中VEGF的表达水平分别为对照组的98.3%-91.9%,与PGE2组相比均有统计学差异(P < 0.01)-结论:PGE2可上调HuH7细胞VEGF的表达,其作用机制可能是通过EP2受体激活cAMP-PKA信号转导通路所致-

摘要:目的:研究肝细胞癌(hepatocelluar carcinoma,HCC)中高迁移率族蛋白A1(high mobility group protein A1,HMGA1)的表达情况,探讨HMGA1基因在HCC中的表达与肝内转移以及临床病理参数之间的关系-方法:采用实时定量荧光聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,QRT-PCR)检测52例肝细胞癌组织和配对的癌旁组织-10例正常肝脏组织中HMGA1 mRNA的表达,用免疫组化法(immunohistochemistry,IHC)检测HMGA1蛋白在肝细胞癌中的表达,评价HMGA1的表达与HCC临床病理学因素的相关性-结果:肝癌组织中HMGA1 mRNA的表达较正常肝脏中升高6.82倍,其中有-无肝内转移组较正常肝组织分别升高13.36倍和4.63倍(P < 0.05)-HMGA1 mRNA的表达与TNM分期-Edmonson分级-肝内转移有关(P < 0.05)-肝细胞癌中有肝内转移组较无肝内转移组HMGA1蛋白表达明显增强-结论:HMGA1基因在肝细胞癌中的表达较正常肝脏组织中明显升高,并与肿瘤的肝内转移相关,有望成为肝细胞癌诊断和治疗的新靶点-

摘要:目的:探讨Fascin蛋白在人肺癌组织中的表达及临床意义-方法:收集63例肺癌标本及相关临床病理资料,以Western blot方法检测肺癌组织-癌旁组织及正常组织中Fascin蛋白的表达情况-结果:Fascin蛋白在肺癌组织中的表达明显高于其在肺正常组织中的表达(P < 0.05),而其表达与肺癌的临床分期(P < 0.05)-淋巴结转移情况(P < 0.05)-组织分化程度(P < 0.05)有关;在肺癌的分型中,Fascin蛋白在鳞癌与腺癌之间的表达水平有显著性差异(P < 0.05);Fascin蛋白的表达与患者的性别-年龄-吸烟史无关(P > 0.05)-结论:Fascin蛋白在肺癌中的高表达,是肺癌分化差-恶性程度高的标志,并与淋巴结转移有关,以Fascin蛋白为靶点可能为肺癌的治疗提供新的思路-

摘要:目的:从临床乳腺癌组织中扩增肿瘤相关抗原Trop-2胞外肽段基因,在大肠杆菌中表达重组蛋白,并对该蛋白的生物学特性进行鉴定-方法:RT-PCR扩增Trop-2胞外肽段基因并克隆于pMD18-T载体中进行测序分析,用NcoⅠ和EcoRⅠ将含Trop-2 胞外区重组基因的质粒双酶切后,克隆到pBAD-gⅢ中-重组质粒经酶切鉴定-核酸序列分析后转化大肠杆菌TOP10,以L-阿拉伯糖诱导表达融合蛋白,表达的蛋白经后His柱亲和层析纯化后,用Western blot-ELISA检测鉴定-结果:重组表达质粒经酶切鉴定为阳性,核酸序列分析正确-SDS-PAGE 和Western blot 显示,表达的重组蛋白分子质量约为38 ku,此抗原能够与商业化抗体特异性结合-结论:成功制备Trop-2胞外区重组基因表达的蛋白,并证明原核表达的Trop-2胞外肽段保持了原有的抗原性-

摘要:目的:探讨外周血单个核细胞(PBMCs)经CD3-CD28双信号通路活化后白细胞介素17A(IL-17A)的分泌以及免疫调节剂雷公藤甲素对该细胞因子分泌的影响-方法:抗CD3单克隆抗体(浓度10 μg/ml)和抗CD28单克隆抗体(浓度5 μg/ml)共刺激PBMCs,模拟T细胞在体内受双信号免疫活化的过程,应用酶联免疫吸附方法(ELISA)检测细胞培养液上清IL-17A水平-结果:外周血单个核细胞经CD3/CD28双信号通路刺激后,其分泌的IL-17A在6 h内即出现明显增加,并在24 h内呈继续增高的趋势;100 nmol/L和500 nmol/L浓度的雷公藤甲素均能够显著抑制该细胞分泌IL-17A-结论:PBMCs经双信号免疫活化后,其分泌的IL-17A明显增加,可能是哮喘气道变应性炎症的形成和发展机制之一;雷公藤甲素能够通过抑制PBMCs对IL-17A分泌,从而有可能减轻哮喘气道炎症-

摘要:目的:观察白细胞介素6(IL-6)对人单核/巨噬细胞系(THP-1)细胞表面Toll样受体4(TLR4)表达的影响-方法:流式细胞仪检测不同浓度IL-6(0 ng/L-10 ng/L-100 ng/L-1 μg/L-10 μg/L-100 μg/L)干预THP-1细胞24 h,以及10 μg/L的IL-6干预THP-1细胞不同的时间(0-3-6-12-24-48 h)后细胞表面TLR4的表达水平-结果:各浓度IL-6均能不同程度的促进细胞表面TLR4的表达(P < 0.05),以10 μg/L 组干预最为显著(P < 0.01);10 μg/L IL-6干预不同的时间后均可促进其表达,以干预24 h后的差异最显著(P < 0.01)-结论:IL-6可以促进人THP-1细胞表面TLR4表达,且具有一定的浓度和时间依赖性-

摘要:目的:探讨在不同成熟状态下树突状细胞(dendritic cell,DC)分泌的外泌体(exosomes,Dex)的制备-提取及体外功能鉴定-方法:F344大鼠骨髓来源DC培养6后分为2组,一组为未成熟树突状细胞组(immature DC,imDC):直接收集上清;一组为成熟树突状细胞组(mature DC,mDC):每孔加入终浓度为1 μg/ml的LPS培养48 h后,收集上清-然后用多步离心法分离得到Dex,流式细胞术检测两组Dex的表型,酶联免疫吸附测定(ELISA)检测两组Dex与Wistar大鼠骨髓来源的imDC共培养上清的白细胞介素10(IL-10)- 白细胞介素12(IL-12p70)的含量,混合淋巴细胞实验(MLR)检测共培养DCs刺激T 细胞增殖能力-结果:与mDC分泌的exosomes相比,imDC分泌的exosomes表面中度表达MHC-Ⅱ类分子-低表达CD80-CD86-CD40共刺激分子,而且与Wistar大鼠骨髓来源的imDC共培养后刺激IL-12p70分泌能力较低(P < 0.05),刺激T淋巴细胞增殖的能力也较mDC分泌的exosomes组弱(P < 0.05)-结论:不同成熟状态下的DC分泌的exosomes功能各异,可能在宿主的免疫反应中发挥不同的作用-

摘要:目的:观察嗅鞘细胞(olfactory ensheathing cells,OECs)条件培养液对PC12细胞神经丝蛋白(neurofilament protein,NF)-突触蛋白(synapsin)和突触素蛋白(synaptophysin)表达的影响,探讨嗅鞘细胞条件培养液促神经细胞分化生长的作用机制-方法:原代培养并纯化OECs,收集培养上清制备成嗅鞘细胞培养液(OEC culture medium,OECCM),与PC12细胞培养24-48-72 h,观察细胞形态学变化;通过免疫荧光法检测PC12细胞NF的表达及分布;蛋白质印迹法检测NF-突触蛋白和突触素的相对含量-结果:OECCM培养的PC12细胞长有突起,并随着培养时间的延长,细胞形态酷似神经元;免疫荧光染色显示NF起初在核周分布,OECCM作用后NF的分布范围逐渐增加,其分布面积与β-tubulin分布面积的比值显著增高(P < 0.05)-蛋白质印迹法显示OECCM组NF-突触蛋白(synapsin)和突触素蛋白(synaptophysin)的表达水平明显高于对照组(P < 0.05)-结论:嗅鞘细胞能分泌一些促PC12细胞分化的因子,这些因子能促进PC12细胞NF骨架的组装,并诱导神经元功能蛋白的表达,使该细胞在结构和功能上向神经元分化-

摘要:目的:对比改进的“天花板”组织块培养法与消化培养法培养前脂肪细胞的优劣-方法:两种方法获得的前脂肪细胞,培养3天,显微镜下检测自分化情况,MTT-流式细胞仪检测其增殖情况和细胞周期-培养9天,绘制生长曲线-使用含胰岛素-甲状腺素T3和转铁蛋白(ITT)的无血清培养基诱导获得的前脂肪细胞5天,拍照测细胞直径,油红染色法检测其分化情况-结果:改进的“天花板”组织块培养法培养的第一代自分化存在,但是发生自分化的细胞很少-增殖和分化结果均无显著性差异-但是此法在同等情况下获得细胞较多,所需经费较少-结论:与消化培养法相比较而言,改进的“天花板”组织块培养法是一种更加简单-经济的获得大鼠前脂肪细胞的方法-

摘要:目的:探讨ERRα在脱垂子宫主骶韧带的表达及17-β雌二醇(17-βE2)对其成纤维细胞周期影响-方法:取10例未脱垂子宫主骶韧带组织和10例脱垂子宫主骶韧带组织,使用流式细胞仪定量检测ERRα表达情况-对10例脱垂子宫主骶韧带组织进行原代细胞培养,传代细胞分别用浓度0.1-1.0-10.0-100.0 nmol/L 17-βE2作用48 h,流式细胞仪术分析刺激后细胞的细胞周期,采用增殖指数(PI)进行比较-结果:ERRα在子宫主骶韧带有表达,且未脱垂子宫主骶韧带ERRα表达量高于脱垂子宫主骶韧带的表达量(P < 0.05)-10.0 nmol/L的17-βE2作用组的PI高于对照组(P < 0.05)-而100.0 nmol/L的17-βE2作用组的PI低于对照组(P < 0.05)-结论:脱垂子宫主骶韧带ERRα表达量降低-10.0 nmol/L 17-βE2能促进韧带成纤维细胞生长,100.0 nmol/L 17-βE2能抑制韧带成纤维细胞生长-

摘要:目的:探讨氧化应激在高糖诱导乳鼠心肌细胞凋亡中的作用-方法:分离培养SD乳鼠心肌细胞,分别用10~50 mmol/L终浓度的葡萄糖进行干预,MTT测定各组心肌细胞的生长活力;激光共聚焦显微镜-脱氧核糖核酸转移酶原位缺口末端标记法(TUNEL)和流式细胞术用于观察和检测心肌细胞凋亡;将心肌细胞分为3组:对照组-高糖组和抗氧化剂组,分别检测各组上清液中乳酸脱氢酶(LDH)-丙二醛(MDA)-超氧化物歧化酶(SOD)的含量以反映心肌细胞的氧化应激水平-结果:MTT结果显示高糖能浓度依赖性地抑制心肌细胞的生长活力;激光共聚焦显微镜-TUNEL染色和流式细胞术均表明高糖能诱导心肌细胞凋亡;与对照组相比,高糖组LDH-MDA含量明显增高,SOD含量显著下降,与高糖组相比,抗氧化剂组LDH,MDA含量 明显下降,SOD含量显著升高;流式细胞检测显示高糖组细胞凋亡率较对照组明显增加,而抗氧化剂组细胞凋亡率较高糖组则显著下降-结论:氧化应激是高糖诱导心肌细胞凋亡的一个重要机制-

摘要:目的:观察大豆异黄酮对血管紧张素Ⅱ(AngⅡ)诱导大鼠胸主动脉内皮细胞(RAECs)增殖的影响及机制-方法:组织贴块法培养RAECs,AngⅡ刺激RAECs建立细胞增殖模型-采用MTT法检测细胞增殖,观察不同浓度大豆异黄酮-亚硝基-精氨酸甲酯(L-NAME,100 μmol/L)对AngⅡ诱导RAECs增殖的影响-硝酸还原酶及化学比色法检测细胞上清液中一氧化氮(NO)-一氧化氮合酶(NOS)-诱导型一氧化氮合酶(iNOS)水平-半定量逆转录聚合酶链反应(RT-PCR)检测RAECs iNOS mRNA 的表达-结果:大豆异黄酮在0.1~1.0 μmol/L呈剂量及时间依赖性抑制RAECs增殖,但可被L-NAME部分抵消;大豆异黄酮能升高NO-NOS和iNOS水平,增加iNOS mRNA的表达-结论:大豆异黄酮对AngⅡ诱导的RAECs增殖有抑制作用;而上调iNOS基因表达,升高NO水平可能是其发挥作用的机制-

摘要:目的:探讨迷迭香酸(rosmarinic acid,RA)对醛固酮(aldosterone,ALD)诱导的人肾小管上皮细胞转分化的影响及其作用机制-方法:将体外培养的人肾小管上皮细胞(HK-2),分为5组:正常对照组-ALD (100 nmol/L)诱导组-ALD(100 nmol/L)+RA(5 μg/ml)干预组-ALD (100 nmol/L)+RA(25 μg/ml)干预组-ALD(100 nmol/L)+线粒体呼吸链酶抑制剂rotenone (ROT,10 μmol/L)干预组-应用倒置相差显微镜观察细胞形态学的变化;应用RT-PCR-Western blot检测波形蛋白(Vimentin)-平滑肌肌动蛋白(α-SMA)及钙粘蛋白(E-cadherin)的表达水平;采用Western blot检测细胞胞外信号调节激酶(ERK1/2)的磷酸化水平;DCFDA荧光法定量检测活性氧(ROS)表达情况-结果:①与对照组相比,醛固酮诱导肾小管上皮细胞从原有典型的上皮细胞形态转变为长梭形肌成纤维细胞样形态;Vimentin和α-SMA表达显著上调,E-cadherin表达下降;ERK1/2磷酸化水平增高;活性氧族(ROS)释放显著增高-②不同浓度的RA(5 μg/ml,25 μg/ml)及ROT(10 μmol/L)干预组与ALD (100 nmol/L)诱导组相比,Vimentin mRNA和α-SMA蛋白表达下调,E-cadherin mRNA及蛋白表达均上调;ERK1/2磷酸化水平下降;活性氧族(ROS)表达显著下降-结论:RA能够抑制醛固酮诱导的肾小管上皮细胞-间充质转分化(EMT),RA对EMT的负性调节作用可能是通过抑制ROS引起的ERK1/2信号转导途径实现的-

摘要:目的:探讨邻苯二甲酸二丁酯(DBP)孕期暴露对仔鼠阴茎组织乳酸脱氢酶B(L-lactate dehydrogenase B,LDH-B)的影响,以进一步探讨LDH-B在DBP导致的尿道下裂中的作用机制-方法:将20只受孕大鼠随机分成2组,实验组10只,对照组10只-在大鼠怀孕第14~18天(GD14~18)2组分别给予灌胃,实验组给予DBP每天800 mg/kg染毒孕鼠,对照组给予大豆油每天5 ml,妊娠19天(GD19)分别取出仔鼠阴茎组织提取总蛋白,通过Western-blot及免疫组化检测LDH-B的表达-结果:实验组及对照组阴茎组织中均有LDH-B的表达,实验组的相对表达量为0.078±0.02 (n=10),对照组的相对表达量为0.137±0.03 (n=10),实验组中表达明显减少(P < 0.05)-LDH-B主要定位于大鼠阴茎组织细胞的胞浆中-结论:孕期大鼠暴露邻苯二甲酸二丁酯后仔鼠阴茎组织中LDH-B表达明显减少,可能与DBP造成机体缺氧有关-

摘要:目的:比较生后给予不同剂量地塞米松(dexamethasone,DEX)对大鼠肺组织形态发育的影响-方法:120只新生大鼠随机平均分为3组-小剂量DEX组:生后第5天起连续5天腹腔注射DEX 0.2 mg/(kg·d)-大剂量DEX组:生后第5天起连续5天腹腔注射DEX 0.5 mg/(kg·d)-生理盐水组:生后第5天起连续5天腹腔注射等量生理盐水-各组大鼠分别在生后第10-14-21-45天随机选取10只处死,分离肺组织,通过光镜观察,分析比较各组用药对肺形态发育的影响-结果:两DEX治疗组在生后各时间点每视野肺泡计数均低于生理盐水组(P﹤0.01),平均肺泡表面积均高于生理盐水组(P﹤0.05)-两DEX治疗组平均肺泡间隔厚度在生后第10-14天均低于生理盐水组(P﹤0.01),在生后第21天均高于生理盐水组(P﹤0.05)-结论:生后给DEX能显著促进肺部早期发育,但随着给药剂量的增多,肺泡化受阻现象逐渐明显,最终导致肺泡大而少的“气肿样”改变出现,其影响可持续至成年-

摘要:目的:观察大鼠肺血栓栓塞症中血清-肺血管内皮细胞上P-选择素(P-selectin,Ps)的变化,并探讨血必净注射液对其的影响-方法:颈静脉注入自体血凝块制备动物模型-将105只健康雄性SD大鼠按随机数字表法分为对照组-栓塞组和血必净组-每组又分为术后1 h-3 h-1天-3天-7天 5个时间段,每个时间段7只-留取肺组织和血清,观察各组肺组织病理变化,Western blot法检测肺血管内皮细胞上Ps的蛋白表达水平,ELISA法测血清中可溶性P-选择素(sP-selectin,sPs)含量-结果:肺栓塞后病理可见肺动脉中血栓栓塞,炎症反应明显-肺血管内皮上Ps的蛋白表达水平及血清中sPs含量在栓塞后1 h开始显著增高(P < 0.01)-血必净组经治疗后,病理损伤减轻,肺血管内皮上Ps的蛋白表达水平于1 h后下降,与栓塞组比较,有显著性差异(P < 0.01),血清sPs含量也下降,与栓塞组比较,1天后差异均有统计学意义(P < 0.01或P < 0.05)-结论:血必净注射液可能通过降低肺血栓栓塞时Ps表达,从而减轻炎症和凝血纤溶失衡对肺组织的损害,对肺组织具有一定的保护作用-

摘要:目的:探讨兔急性肺动脉血栓栓塞时血清炎症因子血栓素A2(TXA2)-前列环素(PGI2)和肺血管内皮-支气管和肺泡上皮等部位内皮素1(ET-1)变化和血流动力学指标变化及尿激酶(UK)溶栓的影响-方法:24只大耳白兔随机分为对照组-急性肺栓塞(acute pulmonary thromboembolism,APTE) 模型组-尿激酶(UK)治疗组,每组8只-采用自体血栓回输法建立动物模型-常规于造模前-造模后1-2-4-8 h放免法测定血清TXA2-PGI2,漂浮导管监测血流动力学指标,最后免疫组化法检测肺血管内皮-支气管和肺泡上皮各部位ET-1的表达水平-结果:①放免法测定结果:对照组各时间点无显著差异-模型组1 h升高,4 h达高峰,其后开始降低,1-2-4 h点与对照组比较都有统计学意义(P < 0.01)-UK组1 h升高,2 h达高峰,4 h后开始下降,UK组高峰值较模型组低,1-2-4 h UK组与模型组两者有统计学意义 (P < 0.01)-各组术前和8 h无统计学差异(P > 0.05);②血流动力学监测结果:对照组各时间点肺动脉平均压(PAMP)-中心静脉压(CVP)都无明显变化,模型组及UK组PAMP-CVP栓塞后立即升高,2 h升至最高,后有所下降,1-2-4-8 h模型组和UK组两组与对照组均有统计学差异(P < 0.01);UK组与模型组1-2 h有统计学差异(P < 0.01),4-8 h无统计学差异(P > 0.05);③免疫组化检测显示:模型组ET-1蛋白表达的相对含量显著高于对照组,UK组与对照组比较无统计学差异-结论:细胞因子TXA2-PGI2-ET-1参与了APTE早期肺血管阻力增加,APTE早期溶栓可明显降低TXA2- PGI2- ET-1以及减轻肺血管阻力的增加-

摘要:目的:探讨氯化锂预处理对老年大鼠腹部探查术后空间记忆能力的影响-方法:24只18月龄SD大鼠随机分为3组,即对照组(C)-手术组(O)和氯化锂预处理手术组(L/O),每组8只-L/O 组大鼠腹腔注射氯化锂2 mmol/kg,每天1次,连续7天,O组和C组大鼠给予等量生理盐水,除C组外全部大鼠于第8天建立腹部探查手术模型,术后24 h用Morris水迷宫测试大鼠的空间记忆能力-结果:虽然O组或L/O组大鼠的逃避潜伏期比C组大鼠显著增加(P < 0.05),但是L/O组大鼠的逃避潜伏期较O组大鼠明显改善(P < 0.05);C组大鼠的游泳距离显著短于O组或L/O组(P < 0.05),其中L/O组大鼠的游泳距离显著短于O组(P < 0.05)-结论:老年大鼠行腹部探查术后可发生空间记忆能力的下降,氯化锂预处理后可明显减缓这些变化,提示氯化锂预处理具有防治老年大鼠术后空间记忆能力下降的效应-

摘要:帕金森病动物模型是研究帕金森病病因-病机-病理-症状体征-神经保护-药物治疗的必备工具-在众多帕金森病动物模型中目前应用最广泛的是6-OHDA损毁帕金森大鼠模型和MPTP诱导帕金森小鼠模型-为了明确各自特点,作者将两者制备原理-制作方式-检测实验-稳定性-难易度等考虑因素进行对比及评价,以期在帕金森病研究中更好选择和充分利用-

摘要:目的:研究亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性与甲氨蝶呤对急性淋巴细胞白血病患儿所致毒副反应的关系-方法:筛选急性淋巴细胞白血病患儿98例,运用PCR-RFLP方法分析亚甲基四氢叶酸还原酶基因型,分析此基因型与甲氨蝶呤临床毒副作用的关系-结果:携有突变基因型的患者甲氨蝶呤血药浓度高,发生毒副反应的风险大-结论:MTHFR C677T多态性与ALL患儿大剂量甲氨蝶呤化疗后的毒副作用有关,主要表现为胃肠道反应和骨髓抑制-

摘要:目的:对遗传性非综合征型耳聋患者进行中国人常见的耳聋相关基因的突变分析,以明确其分子病因-方法:门诊收集非综合征耳聋患者22名的外周血样本,常规方法提取基因组DNA,PCR扩增GJB2基因和线粒体12SrRNA基因全序列,以及SLC26A4基因7+8外显子和19外显子序列-所有PCR扩增产物经DNA测序分析,测序结果提交NCBI GenBank数据库进行比对,从而对耳聋相关基因的突变进行分析-结果:22名非综合征耳聋患者中共检测到3种GJB2基因的突变:235delC纯合突变2例;235delC杂合突变3例及176del16bp和299delAT复合突变1例;mtDNA 12SrRNA基因检出有2种已知致病突变:A1555G 2例和C1494T 2例;SLC26A4基因有IVS7-2A>G杂合突变2例,纯合突变1例-结论:PCR扩增结合DNA测序是耳聋基因诊断的经典和有效的方法,能确诊非综合征型耳聋患者的分子病因,为临床诊断和治疗提供依据-但该方法存在不能定性-耗时费力-所需成本昂贵且不能同时对不同基因的多个突变位点进行检测等缺点-因此,临床的耳聋基因诊断,需要操作简便-结果准确-通量高-价格低廉的新手段-

摘要:目的:分析1例甲状腺激素抵抗综合征伴桥本甲状腺炎患者及家系的甲状腺激素β受体(thyroid hormone receptor-β,TRβ)基因的突变情况-方法:收集患者-9例家系成员及随机抽取的9名健康对照者的外周血标本,检测甲状腺功能并提取基因组DNA,PCR扩增TRβ基因的第1~10个外显子,PCR产物纯化后直接测序检测TRβ基因是否发生突变-结果:TRβ基因的10个外显子未发现碱基替换-插入-缺失等突变情况-结论:TRβ基因突变不是RTH致病的唯一原因-

摘要:目的:探讨性激素浓度及其受体和雌激素受体β(ERβ)基因多态性与青年人胃腺癌发生发展的关系-方法:以原发性青年胃腺癌患者男女各108例为实验组,随机抽取住院手术胃溃疡患者男女各80例为对照组,用放射免疫法(RIA)检测两组血清性激素水平;用免疫组织化学法(SP法)检测两组雌激素受体(estrogen receptor,ER)表达状况,并用聚合酶链反应-限制性片断长度多态性技术(PCR-RFLP)检测ERβ基因多态性-结果:实验组血清雄激素高于对照组,但雌激素低于对照组,差异均有统计学意义(P < 0.000 1)-实验组男女性患者,在TNM分期之间差异无统计学意义(P > 0.05),但雄激素递减,雌激素递增;而随分化程度降低-淋巴结转移阴性和阳性之间,差异均有统计学意义(P < 0.01)-对照组ERα和ERβ均不表达,而实验组ERα和ERβ阳性率分别为57.4%(124/216)和15.7% (34/216),经统计学处理具有显著性差异(P < 0.000 1),其中分化程度低-有淋巴结转移者-浸润程度深的阳性率高于分化程度高-无淋巴结转移者-浸润程度浅的,且TNM Ⅲ期高于Ⅰ-Ⅱ期(P < 0.001)-PCR-RFLP检测中,用Rsa I酶切显示实验组RR和Rr基因型显著高于rr型,并以RR基因型最高(P < 0.000 1),且分化程度低-有淋巴结转移和浸润越深者RR高,TNM分期Ⅲ期高于Ⅰ-Ⅱ期(P < 0.000 1);Alu I酶切显示各基因型差异无统计学意义 (P > 0.05)-结论:血清性激素水平改变是影响胃腺癌变和易感性的重要因素之一,ERβ基因多态性可能与胃癌的发生发展密切相关-

摘要:目的:探讨血浆B型钠尿肽(brain natriuretic peptide,BNP)-单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)水平对不稳定型心绞痛(unstable angina,UA)近期预后判断的价值-方法:按Braunwald标准,85例心功能正常的UA患者分为ⅠB组(25例)-ⅡB组(30例)及ⅢB组(30例),并取20例稳定型心绞痛(SAP)患者作为对照组-入院后24 h内抽肘前静脉血,分别行BNP和MCP-1检测-随访30天内主要不良心脏事件(MACE)-结果:UA组BNP及MCP-1均显著高于对照组(P均< 0.05);ⅢB组显著高于ⅠB组和ⅡB组(P均< 0.05)-冠状动脉3支与2支病变组BNP明显高于单支病变组(P均< 0.05);3支病变组MCP-1明显高于2支及单支病变组(P均< 0.05)-Logistic回归分析显示BNP和MCP-1是30天MACE的独立预测因素-结论:血浆BNP-MCP-1水平对心功能正常的UA近期不良预后的评估具有重要意义-

摘要:目的:探讨维持性血液透析患者心血管并发症与炎症反应及氧化应激的关系-方法:选取60例常规血液透析患者(非心血管病变28例,心血管病变42例),采用可见光分光光度法测定MDA-SOD,使用酶标仪检测晚期蛋白质氧化产物(AOPP),选取C反应蛋白(CRP)-白介素-6(IL-6)-白介素-10(IL-10)及肿瘤坏死因子-α(TNF-α)作为炎症指标,分析心血管并发症与氧化应激指标及炎症反应指标的关系-结果:Pearson相关分析表明,CRP与MDA-AOPP呈正相关(r=0.514,r=0.372,P < 0.05),与SOD呈负相关(r=-0.391,P < 0.05)-Logistic回归分析显示,对心血管事件发生与否具影响力的指标是CRP(回归系数为2.032)-结论:①MHD心血管并发症患者存在明显的炎症反应及氧化应激状态;②对心血管事件发生与否具影响力的指标是CRP-

摘要:目的:研究双肺大容量灌洗治疗对煤工尘肺患者肺功能及6分钟步行试验的影响-方法:入选29例已明确诊断为煤工尘肺的患者,行双肺大容量灌洗,并于灌洗前-灌洗后2周分别询问其症状,行6分钟步行试验及肺功能检查-结果:29例煤工尘肺患者在行双肺大容量灌洗后,与灌洗前相比胸闷-呼吸困难明显改善(P < 0.05),但是咳嗽-咳痰等症状的改善无统计学意义(P > 0.05)-患者在行双肺大容量灌洗前肺功能表现为小气道功能受损或阻塞性通气功能障碍者有17人-和灌洗前相比,这组患者最大通气量(MVV)和6分钟步行试验明显改善,分别为(96.8±14.1)% vs (91.2±15.2)%,(448.8±60.7)m vs (422.6±46.2)m,而肺总量(TLC)较灌洗前降低(119.4±15.0% vs 115.8±14.7%,P < 0.05)-结论:双肺大容量灌洗治疗在短时间内可以改善症状,提高患者的肺功能及运动耐量,提高患者的生活质量-

摘要:目的:测定髓过氧化物酶(MPO)在子痫前期患者中的表达,探讨MPO在子痫前期发病中的可能作用-方法:应用生化比色法测定正常妊娠组(31例)-子痫前期轻度组(33例)和重度组(30例)子宫静脉血和外周血中MPO含量,同时应用免疫组化EnVision法测定上述患者胎盘组织中MPO表达水平-结果:①在子宫静脉血中,轻度和重度子痫前期MPO含量明显高于正常妊娠组(P < 0.01),子痫前期重度组高于轻度组(P < 0.01);②在外周血中,轻度和重度子痫前期MPO含量虽高于正常妊娠组,但统计学处理显示无显著性差异(P > 0.05);③在胎盘组织中,轻度和重度子痫前期胎盘组织MPO表达水平明显高于正常妊娠组(P < 0.01),重度组高于轻度组(P < 0.05)-结论:MPO在子宫静脉血和胎盘中的表达水平与子痫前期患者的病情严重程度相关,提示MPO可能在局部通过氧化应激参与了子痫前期的发病-

摘要:目的:探讨妊娠期糖尿病(gestational diabetes mellitus,GDM)妇女在孕晚期的血糖值对基础胎心率等各项参数的影响-方法:选取50例GDM妇女进行前瞻性研究,研究对象均为单胎-妊娠36~40周-无胎儿发育异常-既往无糖尿病史-在妊娠晚期用系统胎心监护仪进行60 min以上的监护,同时在监护前即刻-监护进行后30 min和60 min分别抽取外周血检测血糖值,取3点的血糖平均值-结果:18例GDM妇女(36%)平均血糖值 ≥ 6.7 mmol/L,平均基础胎心率为(141.7±17.6)bpm;32例GDM妇女(64%)平均血糖值<6.7 mmol/L,平均基础胎心率为(128.2±19.1)bpm,两者之间有显著差异(P=0.015)-研究发现胎儿基础胎心率与孕妇血糖值呈明显正相关(P=0.000 1,r=0.574);系统胎心监护所示的短程变异与孕妇血糖值呈明显负相关(P=0.003,r=-0.466)-系统胎心监护所示的其它指标与孕妇血糖值没有明显相关性-结论:GDM妇女孕晚期的血糖升高可使胎儿基础胎心率加快-

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摘要:目的:探讨肺癌骨转移发病特点及骨显像的诊断价值-方法:对231例肺癌疑有骨转移的患者99mTc-MDP 全身骨显像进行回顾性分析-结果:231例肺癌患者中骨转移122例,发生率为52.81%-其中肺腺癌的骨转移发生率为64.91 %,肺鳞癌为41.44%,两者骨转移发生率差异有统计学意义(P < 0.01)-骨转移的部位以胸部多见,其次为脊柱-骨盆-四肢和颅骨-结论:全身骨显像灵敏度高,适于对肺癌骨转移瘤进行筛查;肺腺癌较鳞癌易发生骨转移,骨转移的部位以胸部多见-