恶性胸/腹水游离TS、β-tubulin Ⅲ mRNA的表达及其与化疗药物敏感性的关系
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江苏省卫生科研项目(H201235);南京市医学重点科技发展项目(ZKX12012)


Cell-free TS and β-Tubulin III mRNA expression in malignant effusions and its’ association with chemosensitivity in vitro
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    目的:探索恶性胸腹水上清中游离胸苷酸合成酶(TS)-β-tubulin Ⅲ mRNA的表达,以及游离TS-β-tubulin Ⅲ表达和患者5-氟尿嘧啶(5-FU)-多西紫杉醇药物敏感性的关系-方法:收集43例进展期肿瘤患者的恶性胸/腹水,分离-提取游离核酸,分离肿瘤细胞-实时荧光定量RT-PCR分别检测游离上清和肿瘤细胞中TS和β-tubulin Ⅲ mRNA表达;ATP-TCA方法检测肿瘤细胞5-FU-多西紫杉醇的敏感性-结果:TS和β-tubulin Ⅲ mRNA在恶性胸/腹水游离上清中的检出率为98%-与配对的肿瘤细胞相比,游离上清中TS mRNA表达水平高(P = 0.02)-在胃肠肿瘤患者中,游离上清中TS mRNA表达水平与5-FU药物敏感性相关(P = 0.002)-尽管观察到游离上清中β-tubulin Ⅲ mRNA 高表达者多西紫杉醇药物敏感性高的趋势,但差异没有统计学意义-结论:晚期肿瘤患者恶性胸腹水游离上清中可检测到游离TS-β-tubulin III mRNA的表达-在胃肠肿瘤患者中,游离上清TS mRNA表达水平与5-FU药物敏感相关-

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    Objective:Genetic assessment of biomarkers in appropriate tumor samples remains to be hallmarks of the real-time individualized therapy in malignant diseases. Malignant effusion is one of the most common presentations in advanced solid cancer. The aim of this study was to characterize cell-free mRNA in malignant effusions and evaluate its association with the sensitivity of primary tumor cells to docetaxel and 5-fluorouracil (5-FU). Methods:Forty-three Malignant effusions samples were prospectively collected from 42 patients. After cell-free RNA extraction and separation of tumor cells,TS and β-tubulin Ⅲ mRNA transcripts in both supernatants and tumor cells were characterized by real-time quantitative PCR. ATP-TCA assay was used to analyze the sensitivity of primary tumor cells to docetaxel and 5-FU. Results:Cell-free TS and β-tubulin IIItranscripts were observed in 42 of 43 specimens. Compared with gene expression patterns in paired-tumor cells,TS mRNA was significantly higher in supernatant whereas β-tubulin Ⅲ mRNA remained the same(P = 0.02). Cell-free TS mRNA was inversely associated with in vitro sensitivity of cancer cells to 5-FU in the gastrointestinal cohort(P = 0.002). Although high β-tubulin III mRNA expression in supernatantsappeared is positively related with cancer cells’ sensitivity to docetaxel,the result didn’t reach the statistical significantce. Conclusion:The cell-free mRNA transcripts in malignant effusions were highly detectable and cell-free TS mRNA levels in gastrointestinal patients were correlated with 5-FU sensitivity.

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王立峰,胡 静,李茹恬,孟 谊,禹立霞,钱晓萍,刘宝瑞.恶性胸/腹水游离TS、β-tubulin Ⅲ mRNA的表达及其与化疗药物敏感性的关系[J].南京医科大学学报(自然科学版),2014,(12):1671-1676

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  • 收稿日期:2014-05-29
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  • 在线发布日期: 2014-12-26
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