PNPLA7在脂肪组织中的表达及调控的初步研究
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国家重点研发计划(2018YFA0506904);国家自然科学基金(31271268);江苏省自然科学基金(BK20150991)


The expression and regulation of PNPLA7 in adipocyte
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    摘要:

    目的:明确PNPLA7在小鼠不同组织及营养状态下的表达水平;探索不同因素对PNPLA7表达的调节作用。方法:利用生物信息学初步分析PNPLA7在正常组织器官及细胞系中的表达,荧光定量PCR的方法进一步验证PNPLA7在小鼠不同组织及不同营养状态下的表达情况。免疫印迹法及荧光定量PCR法检测脂肪细胞分化过程中和脂肪细胞在胰岛素及脂肪酸处理下PNPLA7表达水平的变化。结果:PNPLA7主要表达于代谢旺盛的组织或细胞中。在饥饿条件下,小鼠脂肪组织PNPLA7的表达水平比正常饮食条件下高,但小鼠饥饿后又喂食,其脂肪组织PNPLA7的表达水平又降低。脂肪细胞中PNPLA7表达在胰岛素处理时下调,油酸处理时上调。且在脂肪细胞分化过程中,PNPLA7表达前期下降,后期逐渐升高。结论:PNPLA7在脂肪组织中的表达水平较高,脂肪细胞分化过程中其表达水平先下降后升高,且受胰岛素及不饱和脂肪酸调控。

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    Objective:To explore the distributions of PNPLA7 in different tissues of mouse under different nutritional status and its expression levels in the process of adipocyte differentiation as well as insulin or fatty acids treatment on adipocytes. Methods:The distributions of PNPLA7 in different tissues and cell lines were preliminarily analyzed using bioinformatics,and the expressions of PNPLA7 in different tissues of mouse under different nutritional status were further identified by real time PCR. In addition,3T3-L1 cells were induced for differentiation and then the PNPLA7 expressions in the process of differentiation and in the mature adipocytes treated with insulin or fatty acids were detected by real time PCR and Western blot. Results:PNPLA7 was mainly expressed in active metabolic tissues or cells. The expression level of PNPLA7 in adipocytes under starvation was higher than that in normal diet,but became lower when re-fed after starvation status. The expression level was down-regulated in insulin treatment and up-regulated in oleic acids treatment. Besides,the expression of PNPLA7 was down-regulated in the early stage and up-regulated in the later stage of 3T3-L1 cells differentiation. Conclusion:PNPLA7 is enriched in adipocytes and its expression level changes from down-regulation to up-regulation during the adipocyte differentiation. Besides,its expression is regulated by insulin and oil acids.

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蔡丽娥,张 许,季学涛,潘锦堃,厉璐帆,李 仲. PNPLA7在脂肪组织中的表达及调控的初步研究[J].南京医科大学学报(自然科学版),2020,(1):4-9

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  • 收稿日期:2019-06-13
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  • 在线发布日期: 2020-02-09
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