Objective：To investigate the production of drug antibodies in tumor patients treated with cefoperazone and meropenem， and to provide the basis for rational use of antibiotics and accurate medical treatment. Methods：Microcolumn gel method was used to detect the production of antibodies in 501 cases of tumor patients after using cefoperazone and meropenem，and influencing factors were analyzed. Results：The positive rate of cefoperazone drug antibody in tumor patients was 30.54%(124/406)，and the positive rate of meropenem drug antibody was 40.00%(38/95). The cefoperazone and meropenem drug antibody were mainly detected in patients with blood system diseases，of which the positive rates was 36.43% and 40.96% respectively. The results of different age groups showed that the positive rate of cefoperazone drug antibody was the highest in 31~40 years old group，reaching 70.37%. The positive rate of meropenem drug antibody was the highest in 41~50 years old group，reaching 86.67% . Conclusion：The use of cefoperazone and meropenem in tumor patients could induce drug antibodies and the production of drug antibodies is related to disease，gender，and age.