文章摘要
Fang Chen,Xiaohua Zhou,Yan Lin,Changwen Jing,Tao Yang,Yong Ji,Yujie Sun,Xiao Han.[J].南京医科大学学报,2010,(5):381~388
Resveratrol prevents interleukin-1β-induced dysfunction of pancreatic β-cells
投稿时间:2010-01-25  
DOI:10.7655
中文关键词: 
英文关键词: resveratrol, interleukin-1β, peroxisome proliferator-activated receptor-γ, nitric oxide, nuclear factor-κB.
基金项目:
作者单位
Fang Chen Key Laboratory of Human Functional Genomics of Jiangsu Province, Clinical Diabetes Centre of Jiangsu Province, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. 
Xiaohua Zhou Key Laboratory of Human Functional Genomics of Jiangsu Province, Clinical Diabetes Centre of Jiangsu Province, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. 
Yan Lin Key Laboratory of Human Functional Genomics of Jiangsu Province, Clinical Diabetes Centre of Jiangsu Province, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. 
Changwen Jing Key Laboratory of Human Functional Genomics of Jiangsu Province, Clinical Diabetes Centre of Jiangsu Province, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. 
Tao Yang Department of Endocrinology, Jiangsu Diabetes Center, the First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. 
Yong Ji Atherosclerosis Research Centre, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. 
Yujie Sun Key Laboratory of Human Functional Genomics of Jiangsu Province, Clinical Diabetes Centre of Jiangsu Province, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. 
Xiao Han Key Laboratory of Human Functional Genomics of Jiangsu Province, Clinical Diabetes Centre of Jiangsu Province, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. 
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中文摘要:
      
英文摘要:
      Objective: Interleukin-1β (IL-1β) plays an important role in the development of type 1 and type 2 diabetes mellitus. Resveratrol, a polyphenol, is known to have a wide range of pharmacological properties in vitro. In this research, we examined the effects of resveratrol on IL-1β-induced β-cell dysfunction. Methods: We first evalu-ated the effect of resveratrol on nitric oxide (NO) formation in RINm5F cells stimulated with IL-1β using the Griess method. Next, we performed transient transfection and reporter assays to measure the transcriptional activ-ity of peroxisome proliferator-activated receptor-γ (PPAR-γ). We also used Western blotting analysis to assess the effect of resveratrol on inducible nitric oxide synthase (iNOS) expression and nuclear factor-κB (NF-κB) translo-cation to the nuclei in cells treated with IL-1β. In addition, we assessed the transcriptional activity of NF-κB using an electrophoretic mobility shift assay (EMSA). Finally, we evaluated the effect of resveratrol on IL-1β–induced inhibition of glucose-stimulated insulin secretion in freshly isolated rat pancreatic islets. Results: Resveratrol significantly suppressed IL-1β-induced NO production, a finding that correlated well with reduced levels of iNOS mRNA and protein. The molecular mechanism by which resveratrol inhibited iNOS gene expression appeared to involve increased PPAR-γ activity, which resulted in the inhibition of NF-κB activation. Further analysis showed that resveratrol could prevent IL-1β-induced inhibition of glucose-stimulated insulin secretion in rat islets. Con-clusion: In this study, we demonstrated that resveratrol could protect against pancreatic β-cell dysfunction caused by IL-1β.
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