白血病WT1基因表达及其临床意义
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Expression of the Wilms′ Tumor Gene (WT1) in Leukemia Patients and Its Clinical Significance
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    目的 通过体外实验研究人体甲状腺乳头状癌细胞对牛-促甲状腺激素(TSH)、去甲肾上腺素的反应性,阐述它们之间的关系,观察甲状腺癌细胞生长的影响因素,探讨基内分泌治疗的机理。方法 实验标本来自手术病例,病理类型为乳头状癌。修理组织、剪碎,胰酶和胶原酶Ⅰ型双酶消化,96孔板置于37℃、5%CO2、95%湿度孵箱中培养。用不同浓度的bTSH和去甲肾上腺素分别刺激72h;非放射细胞增殖法测定细胞生长。结果

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    Objective To evaluate the relationship between WT1 gene expression and leukemia, as well as its significance. Methods Expression of WT1 gene was measured with RT-PCR in 68 cases of leukemia at different disease-developing stage. Results Expression of WT1 gene was revealed in 65%newly diagnosed and relapsed acute leukemia and 44%chronic myelogenous leukemia (CML). All of 4 patients with CML at blast crisis stage had positive expresion of WT1. As contrast, neither 10 volunteers, nor 10 patients with no-hematological disease were WT1 positive. Conclusion Expression of WT1 gene was revealed in all kinds of leukemia patients and associated with quantity of leukemic cells. RT-PCR was so far the most sensitive method for minimum residual disease (MRD) detection after chemotherapy and bone marrow transplantation. And it was also useful in pathogenesis study and prognotic evaluation in leukemia.

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胡卫星,耿晓增,李麟荪,施海彬,庄振武,王杰,盛瑞兰,夏薇,朱广荣,钱思轩.白血病WT1基因表达及其临床意义[J].南京医科大学学报(自然科学版),2000,20(01):

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