To prepare an animal model which is stable on the pathological changes. The preparation of the animal model is easy and rapid, and is close to the human's glomerulosclerosis in pathology.Methods After excise a kidney, daunomycin was injected into rat's tail vein twice. The changes of their serum, urine and pathological changes was evaluated. Results The rats had edema, heavy proteinuria, hypoproteinemia, hypercholesterolemia and typical glomerular segmental sclerosis in pathology in the fourth week. in the ninth week, the BUN and creatinine lev el significantly increased (P<0.05) ,and the rate of ginmerulosclerosis raised to 80%Conclusion That daunomycin animal model can be used as an experimental mode[ of glomerulosclerosis, and the rapid preparation is one of its best advantages.