文章摘要
姚 欣,戴山林,黄 茂,殷凯生.早期T细胞激活过程中NF-κB活化的研究[J].南京医科大学学报,2006,(11):1015~1017
早期T细胞激活过程中NF-κB活化的研究
Research of NF-?资B in early phase of T cell activation
投稿时间:2006-05-18  
DOI:10.7655
中文关键词: 共刺激  信号转导  核因子-κB  三氧化二砷
英文关键词: co-stimulation  signal transduction  NF-κB  arsenic troxide
基金项目:江苏省自然科学基金创新人才项目(BK2005437),江苏省教育厅自然科学基金项目资助(02KJD320012)
作者单位
姚 欣 南京医科大学第一附属医院呼吸科江苏 南京 210029 
戴山林  
黄 茂  
殷凯生  
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中文摘要:
      目的:研究T细胞在CD3/CD28双信号通路共刺激活化早期,细胞中NF-κB的活化以及三氧化二砷对该活化作用的影响。方法:用CD3/CD28单克隆抗体(浓度分别为10 μg/ml和5 μg/ml)双信号通路对Hut-78细胞进行共刺激30 min,采用TransAM的方法观察Hut-78细胞内p65的表达状况,并研究三氧化二砷对该表达的影响。结果:Hut-78细胞在CD3/CD28双信号通路共刺激30 min后,细胞核中p65的表达显著下降(P < 0.05)。三氧化二砷组p65表达要高于单纯CD3/CD28作用组。全细胞蛋白提取液中的p65表达水平的趋势与细胞核提取液类似。结论:T细胞经过CD3/CD28共刺激,细胞内NF-κB不能得到迅速活化,反而有下降的趋势。三氧化二砷能够促进NF-κB的早期活化。
英文摘要:
      Objective: To study NF-κB activation during the early phase of T cell reaction due to CD3/CD28 co-stimulation and the effect of arsenic trioxide on NF-κB. Methods: Hut-78 cells were stimulated with CD3/CD28 monoclonal antibodies(10 μg/ml and 5 μg/ml, respectively) in 30 min. TransAM was employed to investigate p65 expression in nuclear extracts and the effect of arsenic troxide. Results: The level of p65 in nuclear extracts of Hut-78 cells was much lower during the CD3/CD28 co-stimulation in 30 min, however arsenic trioxide seemed to inhibit the effect of CD3/CD28 co-stimulation. And the trend was nearly the same in whole cell lysates. Conclusion: NF-κB is suppressed during the early phase of CD3/CD28 co-stimulation in human T cells.
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