11β-类固醇脱氢酶Ⅰ型基因微卫星多态性与2型糖尿病的相关性研究
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江苏省卫生厅医学科技发展基金重点课题资助(H200128)


Relationship between microsatellite marker within the gene encoding human 11-hydroxysteroid dehydrogenases type 1(11β-HSD1)and type 2 diabetes
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    目的: 探讨南京地区汉族人群中11β-类固醇脱氢酶Ⅰ型基因多态性与2型糖尿病的相关性。方法: 选择11β-类固醇脱氢酶Ⅰ型基因内含子4内高杂合度微卫星DNA多态标记,应用聚合酶链反应及变性聚丙烯酰胺凝胶电泳技术,探讨这一微卫星多态在中国南京汉族人群121例正常健康者和150例2型糖尿病患者之间等位基因频率分布差异。结果: 该多态位点存在8种等位基因,CA重复序列分别重复13、14、15、16、17、18、19和20次,病例-对照群体分析结果表明,两组间等位基因频率总体分布差异无显著性(χ2 = 8.9944, Ρ = 0.253)。但(CA)15等位基因糖尿病组频率高于正常组,有统计学意义(χ2 = 4.990,P = 0.025),可以初步认为(CA)15等位基因可能是糖尿病发生的危险因素。结论: 南京地区汉族人群中11β-类固醇脱氢酶Ⅰ型基因多态性可能与2型糖尿病有一定关系。

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    Objective: To explore the relationship between microsatellite DNA polymorphism of human 11beta-hydroxysteroid dehydrogenases type 1 and type 2 diabetes within a Chinese population. Methods:150 patients with type 2 diabetes and 121 nondiabetic subjects were collected randomly among Han Chinese in Nanjing. Polymorphism of microsatellite marker within intron 4 of 11β-HSD1 was detected by polymerase chain reaction, polyacrylamide gel electrophoresis and silver staining to investigate the frequency distributions of 11beta-HSD1 microsatellite(CA)n in two groups. Results: In two groups, CA-repeat numbers in eight types of alleles were 13,14,15,16,17,18,19 and 20, respectively. The prevalence of 11beta-HSD gene allele frequencies in patients with type 2 diabetes was not significantly different from that in control subjects(χ2 = 8.9944, P = 0.253). However, the prevalence of (CA)15 allele gene frequencies in patients with type 2 diabetes was significantly different from that in control subjects(χ2 = 4.990,P = 0.025). Conclusion: Polymorphism of microsatellite markers in 11beta-HSD1 may be associated with type 2 diabetes in Han Chinese.

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王继荣,李慧敏,张 鹏,鲁一兵,蒋秀琴,缪 珩.11β-类固醇脱氢酶Ⅰ型基因微卫星多态性与2型糖尿病的相关性研究[J].南京医科大学学报(自然科学版),2006,(11):1038-1042

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  • 收稿日期:2006-05-24
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